Non-enzymatic DNA cleavage reaction induced by 5-ethynyluracil in methylamine aqueous solution and application to DNA concatenation

DNA can be concatenated by hybridization of DNA fragments with protruding single-stranded termini. DNA cleavage occurring at a nucleotide containing a DNA base analogue is a useful method to obtain DNA with designed protruding termini. Here, we report a novel non-enzymatic DNA cleavage reaction for...

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Veröffentlicht in:	PloS one 2014-03, Vol.9 (3), p.e92369-e92369
Hauptverfasser:	Ikeda, Shuji, Tainaka, Kazuki, Matsumoto, Katsuhiko, Shinohara, Yuta, Ode, Koji L, Susaki, Etsuo A, Ueda, Hiroki R
Format:	Artikel
Sprache:	eng
Schlagworte:	Aqueous solutions Biology and life sciences Cleavage Deoxyribonucleic acid DNA DNA Cleavage DNA, Concatenated - chemistry Enzymes Fragmentation Fragments Genetic research Hybridization Laboratories Medicine Methylamine Pharmacology Physical Sciences Single-stranded DNA Synthetic biology Uracil - analogs & derivatives Uracil - chemistry
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