Corneal alterations induced by topical application of commercial latanoprost, travoprost and bimatoprost in rabbit
Prostaglandin (PG) analogs, including latanoprost, travoprost, and bimatoprost, are currently the most commonly used topical ocular hypotensive medications. The purpose of this study was to investigate the corneal alterations in rabbits following exposure to commercial solution of latanoprost, travo...
Gespeichert in:
| Veröffentlicht in: | PloS one 2014-03, Vol.9 (3), p.e89205-e89205 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e89205 |
|---|---|
| container_issue | 3 |
| container_start_page | e89205 |
| container_title | PloS one |
| container_volume | 9 |
| creator | Chen, Wensheng Dong, Nuo Huang, Caihong Zhang, Zhenhao Hu, Jiaoyue Xie, Hui Pan, Juxin Liu, Zuguo |
| description | Prostaglandin (PG) analogs, including latanoprost, travoprost, and bimatoprost, are currently the most commonly used topical ocular hypotensive medications. The purpose of this study was to investigate the corneal alterations in rabbits following exposure to commercial solution of latanoprost, travoprost and bimatoprost. A total of 64 New Zealand albino rabbits were used and four groups of treatments were constituted. Commercial latanoprost, travoprost, bimatoprost or 0.02% benzalkonium chloride (BAK) was applied once daily to one eye each of rabbits for 30 days. The contralateral untreated eyes used as controls. Schirmer test, tear break-up time (BUT), rose Bengal and fluorescein staining were performed on days 5, 10, 20, and 30. Central corneal changes were analyzed by in vivo confocal microscopy, and the corneal barrier function was evaluated by measurement of corneal transepithelial electrical resistance on day 5. Whole mount corneas were analyzed by using fluorescence confocal microscopy for the presence of tight-junction (ZO-1, occludin) and adherens-junction (E-cadherin, β-catenin) proteins, actin cytoskeleton, proliferative marker Ki67 and cell apoptosis in the epithelium. Topical application of commercial PG analogs resulted in significant corneal epithelial and stromal defects while no significant changes in aqueous tear production, BUT, rose bengal and fluorescein staining scores on day 5. Commercial PG analogs induced dislocation of ZO-1 and occludin from their normal locus, disorganization of cortical actin cytoskeleton at the superficial layer, and disruption of epithelial barrier function. The eyes treated with 0.02% BAK and latanoprost exhibited significantly reduced Schirmer scores, BUT, and increased fluorescein staining scores on days 10 and 30, respectively. Topical application of commercial PG analogs can quickly impair the corneal epithelium and stroma without tear deficiency. Commercial PG analogs break down the barrier integrity of corneal epithelium, concomitant with the disruption of cell junction and actin cytoskeleton between superficial cells in the corneal epithelium in vivo. |
| doi_str_mv | 10.1371/journal.pone.0089205 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1507595595</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478763104</galeid><doaj_id>oai_doaj_org_article_a350fb14fa684d03b938a77046ae73cd</doaj_id><sourcerecordid>A478763104</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-b49fe3b8ea1ff2fa5677bc4de1016048021e58f8432ef4be3a513c479f5242c63</originalsourceid><addsrcrecordid>eNqNk9tq3DAQhk1padK0b1BaQ6G00N3qaMs3hbD0sBAI9HQrZFnaVdBKjiSH5u0r7zphXXJRbPBI882v0XimKF5CsIS4hh-v_BCcsMveO7UEgDUI0EfFKWwwWlQI4MdH9knxLMYrAChmVfW0OEGkwohSdlqElQ9OCVsKm1QQyXgXS-O6QaqubG_L5HsjR3ff22yM_tLrUvrdTgVpsseKJJzvg4_pQ5mCuDnYpXBZwOxEmtbGlUG0rUnPiyda2KheTN-z4teXzz9X3xYXl1_Xq_OLhawpS4uWNFrhlikBtUZa0KquW0k6BQGsAGEAQUWZZgQjpUmrsKAQS1I3miKCZIXPitcH3d76yKdyRQ4pqGlD85uJ9YHovLjifcjZhlvuheH7DR82XIRkpFVcYAp0C4kWFSMdwG2DmahrQCqhaiy7rPVpOm1od6qTyuVa2Jno3OPMlm_8DccNJZSOybybBIK_HlRMfGeiVNYKp_ywz5sRRBqCMvrmH_Th203URuQLGKd9PleOovyc1KyuMAQkU8sHqPx0amdk7i1t8v4s4P0sIDNJ_UkbMcTI1z--_z97-XvOvj1it7kn0zZ6O-xbcg6SAyhzX8Wg9H2RIeDjaNxVg4-jwafRyGGvjn_QfdDdLOC_vSQK4g</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1507595595</pqid></control><display><type>article</type><title>Corneal alterations induced by topical application of commercial latanoprost, travoprost and bimatoprost in rabbit</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Chen, Wensheng ; Dong, Nuo ; Huang, Caihong ; Zhang, Zhenhao ; Hu, Jiaoyue ; Xie, Hui ; Pan, Juxin ; Liu, Zuguo</creator><contributor>Ljubimov, Alexander V.</contributor><creatorcontrib>Chen, Wensheng ; Dong, Nuo ; Huang, Caihong ; Zhang, Zhenhao ; Hu, Jiaoyue ; Xie, Hui ; Pan, Juxin ; Liu, Zuguo ; Ljubimov, Alexander V.</creatorcontrib><description>Prostaglandin (PG) analogs, including latanoprost, travoprost, and bimatoprost, are currently the most commonly used topical ocular hypotensive medications. The purpose of this study was to investigate the corneal alterations in rabbits following exposure to commercial solution of latanoprost, travoprost and bimatoprost. A total of 64 New Zealand albino rabbits were used and four groups of treatments were constituted. Commercial latanoprost, travoprost, bimatoprost or 0.02% benzalkonium chloride (BAK) was applied once daily to one eye each of rabbits for 30 days. The contralateral untreated eyes used as controls. Schirmer test, tear break-up time (BUT), rose Bengal and fluorescein staining were performed on days 5, 10, 20, and 30. Central corneal changes were analyzed by in vivo confocal microscopy, and the corneal barrier function was evaluated by measurement of corneal transepithelial electrical resistance on day 5. Whole mount corneas were analyzed by using fluorescence confocal microscopy for the presence of tight-junction (ZO-1, occludin) and adherens-junction (E-cadherin, β-catenin) proteins, actin cytoskeleton, proliferative marker Ki67 and cell apoptosis in the epithelium. Topical application of commercial PG analogs resulted in significant corneal epithelial and stromal defects while no significant changes in aqueous tear production, BUT, rose bengal and fluorescein staining scores on day 5. Commercial PG analogs induced dislocation of ZO-1 and occludin from their normal locus, disorganization of cortical actin cytoskeleton at the superficial layer, and disruption of epithelial barrier function. The eyes treated with 0.02% BAK and latanoprost exhibited significantly reduced Schirmer scores, BUT, and increased fluorescein staining scores on days 10 and 30, respectively. Topical application of commercial PG analogs can quickly impair the corneal epithelium and stroma without tear deficiency. Commercial PG analogs break down the barrier integrity of corneal epithelium, concomitant with the disruption of cell junction and actin cytoskeleton between superficial cells in the corneal epithelium in vivo.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0089205</identifier><identifier>PMID: 24632558</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Administration, Topical ; Amides - administration & dosage ; Amides - pharmacology ; Analogs ; Animals ; Apoptosis ; Bimatoprost ; Biology ; Cloprostenol - administration & dosage ; Cloprostenol - analogs & derivatives ; Cloprostenol - pharmacology ; Cornea - drug effects ; E-cadherin ; Fluorescein ; Fluorescence microscopy ; Latanoprost ; Male ; Medicine ; Muscle proteins ; Prostaglandins ; Prostaglandins F, Synthetic - administration & dosage ; Prostaglandins F, Synthetic - pharmacology ; Rabbits ; Travoprost ; Veterinary Science</subject><ispartof>PloS one, 2014-03, Vol.9 (3), p.e89205-e89205</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Chen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Chen et al 2014 Chen et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-b49fe3b8ea1ff2fa5677bc4de1016048021e58f8432ef4be3a513c479f5242c63</citedby><cites>FETCH-LOGICAL-c758t-b49fe3b8ea1ff2fa5677bc4de1016048021e58f8432ef4be3a513c479f5242c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3954555/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3954555/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24632558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ljubimov, Alexander V.</contributor><creatorcontrib>Chen, Wensheng</creatorcontrib><creatorcontrib>Dong, Nuo</creatorcontrib><creatorcontrib>Huang, Caihong</creatorcontrib><creatorcontrib>Zhang, Zhenhao</creatorcontrib><creatorcontrib>Hu, Jiaoyue</creatorcontrib><creatorcontrib>Xie, Hui</creatorcontrib><creatorcontrib>Pan, Juxin</creatorcontrib><creatorcontrib>Liu, Zuguo</creatorcontrib><title>Corneal alterations induced by topical application of commercial latanoprost, travoprost and bimatoprost in rabbit</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Prostaglandin (PG) analogs, including latanoprost, travoprost, and bimatoprost, are currently the most commonly used topical ocular hypotensive medications. The purpose of this study was to investigate the corneal alterations in rabbits following exposure to commercial solution of latanoprost, travoprost and bimatoprost. A total of 64 New Zealand albino rabbits were used and four groups of treatments were constituted. Commercial latanoprost, travoprost, bimatoprost or 0.02% benzalkonium chloride (BAK) was applied once daily to one eye each of rabbits for 30 days. The contralateral untreated eyes used as controls. Schirmer test, tear break-up time (BUT), rose Bengal and fluorescein staining were performed on days 5, 10, 20, and 30. Central corneal changes were analyzed by in vivo confocal microscopy, and the corneal barrier function was evaluated by measurement of corneal transepithelial electrical resistance on day 5. Whole mount corneas were analyzed by using fluorescence confocal microscopy for the presence of tight-junction (ZO-1, occludin) and adherens-junction (E-cadherin, β-catenin) proteins, actin cytoskeleton, proliferative marker Ki67 and cell apoptosis in the epithelium. Topical application of commercial PG analogs resulted in significant corneal epithelial and stromal defects while no significant changes in aqueous tear production, BUT, rose bengal and fluorescein staining scores on day 5. Commercial PG analogs induced dislocation of ZO-1 and occludin from their normal locus, disorganization of cortical actin cytoskeleton at the superficial layer, and disruption of epithelial barrier function. The eyes treated with 0.02% BAK and latanoprost exhibited significantly reduced Schirmer scores, BUT, and increased fluorescein staining scores on days 10 and 30, respectively. Topical application of commercial PG analogs can quickly impair the corneal epithelium and stroma without tear deficiency. Commercial PG analogs break down the barrier integrity of corneal epithelium, concomitant with the disruption of cell junction and actin cytoskeleton between superficial cells in the corneal epithelium in vivo.</description><subject>Administration, Topical</subject><subject>Amides - administration & dosage</subject><subject>Amides - pharmacology</subject><subject>Analogs</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Bimatoprost</subject><subject>Biology</subject><subject>Cloprostenol - administration & dosage</subject><subject>Cloprostenol - analogs & derivatives</subject><subject>Cloprostenol - pharmacology</subject><subject>Cornea - drug effects</subject><subject>E-cadherin</subject><subject>Fluorescein</subject><subject>Fluorescence microscopy</subject><subject>Latanoprost</subject><subject>Male</subject><subject>Medicine</subject><subject>Muscle proteins</subject><subject>Prostaglandins</subject><subject>Prostaglandins F, Synthetic - administration & dosage</subject><subject>Prostaglandins F, Synthetic - pharmacology</subject><subject>Rabbits</subject><subject>Travoprost</subject><subject>Veterinary Science</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tq3DAQhk1padK0b1BaQ6G00N3qaMs3hbD0sBAI9HQrZFnaVdBKjiSH5u0r7zphXXJRbPBI882v0XimKF5CsIS4hh-v_BCcsMveO7UEgDUI0EfFKWwwWlQI4MdH9knxLMYrAChmVfW0OEGkwohSdlqElQ9OCVsKm1QQyXgXS-O6QaqubG_L5HsjR3ff22yM_tLrUvrdTgVpsseKJJzvg4_pQ5mCuDnYpXBZwOxEmtbGlUG0rUnPiyda2KheTN-z4teXzz9X3xYXl1_Xq_OLhawpS4uWNFrhlikBtUZa0KquW0k6BQGsAGEAQUWZZgQjpUmrsKAQS1I3miKCZIXPitcH3d76yKdyRQ4pqGlD85uJ9YHovLjifcjZhlvuheH7DR82XIRkpFVcYAp0C4kWFSMdwG2DmahrQCqhaiy7rPVpOm1od6qTyuVa2Jno3OPMlm_8DccNJZSOybybBIK_HlRMfGeiVNYKp_ywz5sRRBqCMvrmH_Th203URuQLGKd9PleOovyc1KyuMAQkU8sHqPx0amdk7i1t8v4s4P0sIDNJ_UkbMcTI1z--_z97-XvOvj1it7kn0zZ6O-xbcg6SAyhzX8Wg9H2RIeDjaNxVg4-jwafRyGGvjn_QfdDdLOC_vSQK4g</recordid><startdate>20140314</startdate><enddate>20140314</enddate><creator>Chen, Wensheng</creator><creator>Dong, Nuo</creator><creator>Huang, Caihong</creator><creator>Zhang, Zhenhao</creator><creator>Hu, Jiaoyue</creator><creator>Xie, Hui</creator><creator>Pan, Juxin</creator><creator>Liu, Zuguo</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140314</creationdate><title>Corneal alterations induced by topical application of commercial latanoprost, travoprost and bimatoprost in rabbit</title><author>Chen, Wensheng ; Dong, Nuo ; Huang, Caihong ; Zhang, Zhenhao ; Hu, Jiaoyue ; Xie, Hui ; Pan, Juxin ; Liu, Zuguo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-b49fe3b8ea1ff2fa5677bc4de1016048021e58f8432ef4be3a513c479f5242c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Administration, Topical</topic><topic>Amides - administration & dosage</topic><topic>Amides - pharmacology</topic><topic>Analogs</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Bimatoprost</topic><topic>Biology</topic><topic>Cloprostenol - administration & dosage</topic><topic>Cloprostenol - analogs & derivatives</topic><topic>Cloprostenol - pharmacology</topic><topic>Cornea - drug effects</topic><topic>E-cadherin</topic><topic>Fluorescein</topic><topic>Fluorescence microscopy</topic><topic>Latanoprost</topic><topic>Male</topic><topic>Medicine</topic><topic>Muscle proteins</topic><topic>Prostaglandins</topic><topic>Prostaglandins F, Synthetic - administration & dosage</topic><topic>Prostaglandins F, Synthetic - pharmacology</topic><topic>Rabbits</topic><topic>Travoprost</topic><topic>Veterinary Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Wensheng</creatorcontrib><creatorcontrib>Dong, Nuo</creatorcontrib><creatorcontrib>Huang, Caihong</creatorcontrib><creatorcontrib>Zhang, Zhenhao</creatorcontrib><creatorcontrib>Hu, Jiaoyue</creatorcontrib><creatorcontrib>Xie, Hui</creatorcontrib><creatorcontrib>Pan, Juxin</creatorcontrib><creatorcontrib>Liu, Zuguo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Wensheng</au><au>Dong, Nuo</au><au>Huang, Caihong</au><au>Zhang, Zhenhao</au><au>Hu, Jiaoyue</au><au>Xie, Hui</au><au>Pan, Juxin</au><au>Liu, Zuguo</au><au>Ljubimov, Alexander V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Corneal alterations induced by topical application of commercial latanoprost, travoprost and bimatoprost in rabbit</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-03-14</date><risdate>2014</risdate><volume>9</volume><issue>3</issue><spage>e89205</spage><epage>e89205</epage><pages>e89205-e89205</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Prostaglandin (PG) analogs, including latanoprost, travoprost, and bimatoprost, are currently the most commonly used topical ocular hypotensive medications. The purpose of this study was to investigate the corneal alterations in rabbits following exposure to commercial solution of latanoprost, travoprost and bimatoprost. A total of 64 New Zealand albino rabbits were used and four groups of treatments were constituted. Commercial latanoprost, travoprost, bimatoprost or 0.02% benzalkonium chloride (BAK) was applied once daily to one eye each of rabbits for 30 days. The contralateral untreated eyes used as controls. Schirmer test, tear break-up time (BUT), rose Bengal and fluorescein staining were performed on days 5, 10, 20, and 30. Central corneal changes were analyzed by in vivo confocal microscopy, and the corneal barrier function was evaluated by measurement of corneal transepithelial electrical resistance on day 5. Whole mount corneas were analyzed by using fluorescence confocal microscopy for the presence of tight-junction (ZO-1, occludin) and adherens-junction (E-cadherin, β-catenin) proteins, actin cytoskeleton, proliferative marker Ki67 and cell apoptosis in the epithelium. Topical application of commercial PG analogs resulted in significant corneal epithelial and stromal defects while no significant changes in aqueous tear production, BUT, rose bengal and fluorescein staining scores on day 5. Commercial PG analogs induced dislocation of ZO-1 and occludin from their normal locus, disorganization of cortical actin cytoskeleton at the superficial layer, and disruption of epithelial barrier function. The eyes treated with 0.02% BAK and latanoprost exhibited significantly reduced Schirmer scores, BUT, and increased fluorescein staining scores on days 10 and 30, respectively. Topical application of commercial PG analogs can quickly impair the corneal epithelium and stroma without tear deficiency. Commercial PG analogs break down the barrier integrity of corneal epithelium, concomitant with the disruption of cell junction and actin cytoskeleton between superficial cells in the corneal epithelium in vivo.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24632558</pmid><doi>10.1371/journal.pone.0089205</doi><tpages>e89205</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-03, Vol.9 (3), p.e89205-e89205 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1507595595 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Administration, Topical Amides - administration & dosage Amides - pharmacology Analogs Animals Apoptosis Bimatoprost Biology Cloprostenol - administration & dosage Cloprostenol - analogs & derivatives Cloprostenol - pharmacology Cornea - drug effects E-cadherin Fluorescein Fluorescence microscopy Latanoprost Male Medicine Muscle proteins Prostaglandins Prostaglandins F, Synthetic - administration & dosage Prostaglandins F, Synthetic - pharmacology Rabbits Travoprost Veterinary Science |
| title | Corneal alterations induced by topical application of commercial latanoprost, travoprost and bimatoprost in rabbit |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T08%3A57%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Corneal%20alterations%20induced%20by%20topical%20application%20of%20commercial%20latanoprost,%20travoprost%20and%20bimatoprost%20in%20rabbit&rft.jtitle=PloS%20one&rft.au=Chen,%20Wensheng&rft.date=2014-03-14&rft.volume=9&rft.issue=3&rft.spage=e89205&rft.epage=e89205&rft.pages=e89205-e89205&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0089205&rft_dat=%3Cgale_plos_%3EA478763104%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1507595595&rft_id=info:pmid/24632558&rft_galeid=A478763104&rft_doaj_id=oai_doaj_org_article_a350fb14fa684d03b938a77046ae73cd&rfr_iscdi=true |