Chromosomal mapping of repetitive DNAs in Triportheus trifurcatus (Characidae, Characiformes): insights into the differentiation of the Z and W chromosomes
Repetitive DNA sequences play an important role in the structural and functional organization of chromosomes, especially in sex chromosome differentiation. The genus Triportheus represents an interesting model for such studies because all of its species analyzed so far contain a ZZ/ZW sex chromosome...
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| description | Repetitive DNA sequences play an important role in the structural and functional organization of chromosomes, especially in sex chromosome differentiation. The genus Triportheus represents an interesting model for such studies because all of its species analyzed so far contain a ZZ/ZW sex chromosome system. A close relationship has been found between the differentiation of the W chromosome and heterochromatinization, with the involvement of different types of repetitive DNA in this process. This study investigated several aspects of this association in the W chromosome of Triportheus trifurcatus (2 n = 52 chromosomes), including the cytogenetic mapping of repetitive DNAs such as telomeric sequences (TTAGGG)n, microsatellites and retrotransposons. A remarkable heterochromatic segment on the W chromosome was observed with a preferential accumulation of (CAC)10, (CAG)10, (CGG)10, (GAA)10 and (TA)15. The retrotransposons Rex1 and Rex3 showed a general distribution pattern in the chromosomes, and Rex6 showed a different distribution on the W chromosome. The telomeric repeat (TTAGGG)n was highly evident in both telomeres of all chromosomes without the occurrence of ITS. Thus, the differentiation of the W chromosome of T. trifurcatus is clearly associated with the formation of heterochromatin and different types of repetitive DNA, suggesting that these elements had a prominent role in this evolutionary process. |
| doi_str_mv | 10.1371/journal.pone.0090946 |
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B.</contributor><creatorcontrib>Yano, Cassia Fernanda ; Poltronieri, Juliana ; Bertollo, Luiz Antonio Carlos ; Artoni, Roberto Ferreira ; Liehr, Thomas ; de Bello Cioffi, Marcelo ; Marais, Gabriel A. B.</creatorcontrib><description>Repetitive DNA sequences play an important role in the structural and functional organization of chromosomes, especially in sex chromosome differentiation. The genus Triportheus represents an interesting model for such studies because all of its species analyzed so far contain a ZZ/ZW sex chromosome system. A close relationship has been found between the differentiation of the W chromosome and heterochromatinization, with the involvement of different types of repetitive DNA in this process. This study investigated several aspects of this association in the W chromosome of Triportheus trifurcatus (2 n = 52 chromosomes), including the cytogenetic mapping of repetitive DNAs such as telomeric sequences (TTAGGG)n, microsatellites and retrotransposons. A remarkable heterochromatic segment on the W chromosome was observed with a preferential accumulation of (CAC)10, (CAG)10, (CGG)10, (GAA)10 and (TA)15. The retrotransposons Rex1 and Rex3 showed a general distribution pattern in the chromosomes, and Rex6 showed a different distribution on the W chromosome. The telomeric repeat (TTAGGG)n was highly evident in both telomeres of all chromosomes without the occurrence of ITS. Thus, the differentiation of the W chromosome of T. trifurcatus is clearly associated with the formation of heterochromatin and different types of repetitive DNA, suggesting that these elements had a prominent role in this evolutionary process.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0090946</identifier><identifier>PMID: 24632562</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Anostomidae ; Biological evolution ; Biology ; Characidae - genetics ; Characiformes ; Chromosome Mapping ; Chromosomes ; Deoxyribonucleic acid ; Differentiation ; DNA ; DNA - genetics ; DNA sequencing ; Evolution ; Fish ; Functional morphology ; Gene mapping ; Gene sequencing ; Genomes ; Heterochromatin ; Leporinus ; Mapping ; Microsatellites ; Nucleotide sequence ; Repetitive Sequences, Nucleic Acid - genetics ; Sex ; Sex Chromosomes - genetics ; Structure-function relationships ; Telomere - genetics ; Telomeres ; Trinucleotide repeats ; Triportheus ; Triportheus trifurcatus ; Vertebrates ; Veterinary Science ; W chromosomes</subject><ispartof>PloS one, 2014-03, Vol.9 (3), p.e90946-e90946</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Yano et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chromosomal mapping of repetitive DNAs in Triportheus trifurcatus (Characidae, Characiformes): insights into the differentiation of the Z and W chromosomes</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-03-14</date><risdate>2014</risdate><volume>9</volume><issue>3</issue><spage>e90946</spage><epage>e90946</epage><pages>e90946-e90946</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Repetitive DNA sequences play an important role in the structural and functional organization of chromosomes, especially in sex chromosome differentiation. The genus Triportheus represents an interesting model for such studies because all of its species analyzed so far contain a ZZ/ZW sex chromosome system. A close relationship has been found between the differentiation of the W chromosome and heterochromatinization, with the involvement of different types of repetitive DNA in this process. This study investigated several aspects of this association in the W chromosome of Triportheus trifurcatus (2 n = 52 chromosomes), including the cytogenetic mapping of repetitive DNAs such as telomeric sequences (TTAGGG)n, microsatellites and retrotransposons. A remarkable heterochromatic segment on the W chromosome was observed with a preferential accumulation of (CAC)10, (CAG)10, (CGG)10, (GAA)10 and (TA)15. The retrotransposons Rex1 and Rex3 showed a general distribution pattern in the chromosomes, and Rex6 showed a different distribution on the W chromosome. The telomeric repeat (TTAGGG)n was highly evident in both telomeres of all chromosomes without the occurrence of ITS. Thus, the differentiation of the W chromosome of T. trifurcatus is clearly associated with the formation of heterochromatin and different types of repetitive DNA, suggesting that these elements had a prominent role in this evolutionary process.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24632562</pmid><doi>10.1371/journal.pone.0090946</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Anostomidae Biological evolution Biology Characidae - genetics Characiformes Chromosome Mapping Chromosomes Deoxyribonucleic acid Differentiation DNA DNA - genetics DNA sequencing Evolution Fish Functional morphology Gene mapping Gene sequencing Genomes Heterochromatin Leporinus Mapping Microsatellites Nucleotide sequence Repetitive Sequences, Nucleic Acid - genetics Sex Sex Chromosomes - genetics Structure-function relationships Telomere - genetics Telomeres Trinucleotide repeats Triportheus Triportheus trifurcatus Vertebrates Veterinary Science W chromosomes |
| title | Chromosomal mapping of repetitive DNAs in Triportheus trifurcatus (Characidae, Characiformes): insights into the differentiation of the Z and W chromosomes |
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