Combining transcriptome assemblies from multiple de novo assemblers in the allo-tetraploid plant Nicotiana benthamiana

Nicotiana benthamiana is an allo-tetraploid plant, which can be challenging for de novo transcriptome assemblies due to homeologous and duplicated gene copies. Transcripts generated from such genes can be distinct yet highly similar in sequence, with markedly differing expression levels. This can le...

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Veröffentlicht in:	PloS one 2014-03, Vol.9 (3), p.e91776-e91776
Hauptverfasser:	Nakasugi, Kenlee, Crowhurst, Ross, Bally, Julia, Waterhouse, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Biology Cluster Analysis Databases, Protein High-Throughput Nucleotide Sequencing - instrumentation High-Throughput Nucleotide Sequencing - methods Molecular Sequence Annotation Nicotiana - genetics Nicotiana benthamiana Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism RNA RNA Interference RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Homology, Amino Acid Tetraploidy Transcriptome - genetics
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