Germline transmission of an embryonic stem cell line derived from BALB/c cataract mice
Mice embryonic stem (ES) cells have enabled the generation of mouse strains with defined mutation(s) in their genome for putative disease loci analysis. In the study of cataract, the complex genetic background of this disease and lack of long-term self-renewal ES cells have hampered the functional r...
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| Veröffentlicht in: | PloS one 2014-03, Vol.9 (3), p.e90707-e90707 |
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| creator | Peng, Xinrong Liu, Tao Shi, Chuanyin Zhang, Liqing Wang, Ying Zhao, Wuyang Jiang, Lihua Wu, Mengchao Zhang, Yong Qian, Qijun |
| description | Mice embryonic stem (ES) cells have enabled the generation of mouse strains with defined mutation(s) in their genome for putative disease loci analysis. In the study of cataract, the complex genetic background of this disease and lack of long-term self-renewal ES cells have hampered the functional researches of cataract-related genes. In this study, we aimed to establish ES cells from inherited cataract mice (BALB/CCat/Cat). Embryos of cataract mice were cultured in chemical-defined N2B27 medium with the presence of two small molecules PD0325901 and CHIR99021 (2i) and an ES cell line (named EH-BES) was successfully established. EH-BES showed long-term self-renewal in 2i medium and maintained capacity of germline transmission. Most importantly, the produced chimera and offspring developed congenital cataract as well. Flow cytometry assay revealed that EH-BES are homogeneous in expression of Oct4 and Rex1in 2i medium, which may account for their self-renewal ability. With long-term self-renewal ability and germline-competent, EH-BES cell line can facilitate genetic and functional researches of cataract-related genes and better address mechanisms of cataract. |
| doi_str_mv | 10.1371/journal.pone.0090707 |
| format | Article |
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In the study of cataract, the complex genetic background of this disease and lack of long-term self-renewal ES cells have hampered the functional researches of cataract-related genes. In this study, we aimed to establish ES cells from inherited cataract mice (BALB/CCat/Cat). Embryos of cataract mice were cultured in chemical-defined N2B27 medium with the presence of two small molecules PD0325901 and CHIR99021 (2i) and an ES cell line (named EH-BES) was successfully established. EH-BES showed long-term self-renewal in 2i medium and maintained capacity of germline transmission. Most importantly, the produced chimera and offspring developed congenital cataract as well. Flow cytometry assay revealed that EH-BES are homogeneous in expression of Oct4 and Rex1in 2i medium, which may account for their self-renewal ability. With long-term self-renewal ability and germline-competent, EH-BES cell line can facilitate genetic and functional researches of cataract-related genes and better address mechanisms of cataract.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0090707</identifier><identifier>PMID: 24595217</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Benzamides - metabolism ; Biology ; Cataract - genetics ; Cataracts ; Cell Line ; Chimera - metabolism ; Chimeras ; Cytometry ; Diphenylamine - analogs & derivatives ; Diphenylamine - metabolism ; Embryo cells ; Embryo Culture Techniques ; Embryonic stem cells ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - metabolism ; Embryos ; Female ; Flow cytometry ; Genes ; Genetic aspects ; Genetic disorders ; Genomes ; Kinases ; Mice ; Mice, Inbred BALB C ; Mutation ; Oct-4 protein ; Offspring ; Pyridines - metabolism ; Pyrimidines - metabolism ; Stem cells ; Veterinary Science</subject><ispartof>PloS one, 2014-03, Vol.9 (3), p.e90707-e90707</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Peng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Peng et al 2014 Peng et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c641t-6ce30e30cf6aae83060afc36735cbefcc2808f721e3517eec58a86e2e26f5d683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3942454/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3942454/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24595217$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peng, Xinrong</creatorcontrib><creatorcontrib>Liu, Tao</creatorcontrib><creatorcontrib>Shi, Chuanyin</creatorcontrib><creatorcontrib>Zhang, Liqing</creatorcontrib><creatorcontrib>Wang, Ying</creatorcontrib><creatorcontrib>Zhao, Wuyang</creatorcontrib><creatorcontrib>Jiang, Lihua</creatorcontrib><creatorcontrib>Wu, Mengchao</creatorcontrib><creatorcontrib>Zhang, Yong</creatorcontrib><creatorcontrib>Qian, Qijun</creatorcontrib><title>Germline transmission of an embryonic stem cell line derived from BALB/c cataract mice</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Mice embryonic stem (ES) cells have enabled the generation of mouse strains with defined mutation(s) in their genome for putative disease loci analysis. In the study of cataract, the complex genetic background of this disease and lack of long-term self-renewal ES cells have hampered the functional researches of cataract-related genes. In this study, we aimed to establish ES cells from inherited cataract mice (BALB/CCat/Cat). Embryos of cataract mice were cultured in chemical-defined N2B27 medium with the presence of two small molecules PD0325901 and CHIR99021 (2i) and an ES cell line (named EH-BES) was successfully established. EH-BES showed long-term self-renewal in 2i medium and maintained capacity of germline transmission. Most importantly, the produced chimera and offspring developed congenital cataract as well. Flow cytometry assay revealed that EH-BES are homogeneous in expression of Oct4 and Rex1in 2i medium, which may account for their self-renewal ability. With long-term self-renewal ability and germline-competent, EH-BES cell line can facilitate genetic and functional researches of cataract-related genes and better address mechanisms of cataract.</description><subject>Analysis</subject><subject>Animals</subject><subject>Benzamides - metabolism</subject><subject>Biology</subject><subject>Cataract - genetics</subject><subject>Cataracts</subject><subject>Cell Line</subject><subject>Chimera - metabolism</subject><subject>Chimeras</subject><subject>Cytometry</subject><subject>Diphenylamine - analogs & derivatives</subject><subject>Diphenylamine - metabolism</subject><subject>Embryo cells</subject><subject>Embryo Culture Techniques</subject><subject>Embryonic stem cells</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - metabolism</subject><subject>Embryos</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic disorders</subject><subject>Genomes</subject><subject>Kinases</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mutation</subject><subject>Oct-4 protein</subject><subject>Offspring</subject><subject>Pyridines - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peng, Xinrong</au><au>Liu, Tao</au><au>Shi, Chuanyin</au><au>Zhang, Liqing</au><au>Wang, Ying</au><au>Zhao, Wuyang</au><au>Jiang, Lihua</au><au>Wu, Mengchao</au><au>Zhang, Yong</au><au>Qian, Qijun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Germline transmission of an embryonic stem cell line derived from BALB/c cataract mice</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-03-04</date><risdate>2014</risdate><volume>9</volume><issue>3</issue><spage>e90707</spage><epage>e90707</epage><pages>e90707-e90707</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Mice embryonic stem (ES) cells have enabled the generation of mouse strains with defined mutation(s) in their genome for putative disease loci analysis. In the study of cataract, the complex genetic background of this disease and lack of long-term self-renewal ES cells have hampered the functional researches of cataract-related genes. In this study, we aimed to establish ES cells from inherited cataract mice (BALB/CCat/Cat). Embryos of cataract mice were cultured in chemical-defined N2B27 medium with the presence of two small molecules PD0325901 and CHIR99021 (2i) and an ES cell line (named EH-BES) was successfully established. EH-BES showed long-term self-renewal in 2i medium and maintained capacity of germline transmission. Most importantly, the produced chimera and offspring developed congenital cataract as well. Flow cytometry assay revealed that EH-BES are homogeneous in expression of Oct4 and Rex1in 2i medium, which may account for their self-renewal ability. With long-term self-renewal ability and germline-competent, EH-BES cell line can facilitate genetic and functional researches of cataract-related genes and better address mechanisms of cataract.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24595217</pmid><doi>10.1371/journal.pone.0090707</doi><tpages>e90707</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Animals Benzamides - metabolism Biology Cataract - genetics Cataracts Cell Line Chimera - metabolism Chimeras Cytometry Diphenylamine - analogs & derivatives Diphenylamine - metabolism Embryo cells Embryo Culture Techniques Embryonic stem cells Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Embryos Female Flow cytometry Genes Genetic aspects Genetic disorders Genomes Kinases Mice Mice, Inbred BALB C Mutation Oct-4 protein Offspring Pyridines - metabolism Pyrimidines - metabolism Stem cells Veterinary Science |
| title | Germline transmission of an embryonic stem cell line derived from BALB/c cataract mice |
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