In site bioimaging of hydrogen sulfide uncovers its pivotal role in regulating nitric oxide-induced lateral root formation
Hydrogen sulfide (H2S) is an important gasotransmitter in mammals. Despite physiological changes induced by exogenous H2S donor NaHS to plants, whether and how H2S works as a true cellular signal in plants need to be examined. A self-developed specific fluorescent probe (WSP-1) was applied to track...
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| Veröffentlicht in: | PloS one 2014-02, Vol.9 (2), p.e90340 |
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| creator | Li, Yan-Jun Chen, Jian Xian, Ming Zhou, Li-Gang Han, Fengxiang X Gan, Li-Jun Shi, Zhi-Qi |
| description | Hydrogen sulfide (H2S) is an important gasotransmitter in mammals. Despite physiological changes induced by exogenous H2S donor NaHS to plants, whether and how H2S works as a true cellular signal in plants need to be examined. A self-developed specific fluorescent probe (WSP-1) was applied to track endogenous H2S in tomato (Solanum lycopersicum) roots in site. Bioimaging combined with pharmacological and biochemical approaches were used to investigate the cross-talk among H2S, nitric oxide (NO), and Ca(2+) in regulating lateral root formation. Endogenous H2S accumulation was clearly associated with primordium initiation and lateral root emergence. NO donor SNP stimulated the generation of endogenous H2S and the expression of the gene coding for the enzyme responsible for endogenous H2S synthesis. Scavenging H2S or inhibiting H2S synthesis partially blocked SNP-induced lateral root formation and the expression of lateral root-related genes. The stimulatory effect of SNP on Ca(2+) accumulation and CaM1 (calmodulin 1) expression could be abolished by inhibiting H2S synthesis. Ca(2+) chelator or Ca(2+) channel blocker attenuated NaHS-induced lateral root formation. Our study confirmed the role of H2S as a cellular signal in plants being a mediator between NO and Ca(2+) in regulating lateral root formation. |
| doi_str_mv | 10.1371/journal.pone.0090340 |
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Despite physiological changes induced by exogenous H2S donor NaHS to plants, whether and how H2S works as a true cellular signal in plants need to be examined. A self-developed specific fluorescent probe (WSP-1) was applied to track endogenous H2S in tomato (Solanum lycopersicum) roots in site. Bioimaging combined with pharmacological and biochemical approaches were used to investigate the cross-talk among H2S, nitric oxide (NO), and Ca(2+) in regulating lateral root formation. Endogenous H2S accumulation was clearly associated with primordium initiation and lateral root emergence. NO donor SNP stimulated the generation of endogenous H2S and the expression of the gene coding for the enzyme responsible for endogenous H2S synthesis. Scavenging H2S or inhibiting H2S synthesis partially blocked SNP-induced lateral root formation and the expression of lateral root-related genes. The stimulatory effect of SNP on Ca(2+) accumulation and CaM1 (calmodulin 1) expression could be abolished by inhibiting H2S synthesis. Ca(2+) chelator or Ca(2+) channel blocker attenuated NaHS-induced lateral root formation. Our study confirmed the role of H2S as a cellular signal in plants being a mediator between NO and Ca(2+) in regulating lateral root formation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0090340</identifier><identifier>PMID: 24587333</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accumulation ; Biology ; Calcium - metabolism ; Calcium binding proteins ; Calcium oxide ; Calcium-binding protein ; Calmodulin ; Calmodulin - metabolism ; Carbon monoxide ; Cell cycle ; Dose-Response Relationship, Drug ; Engineering ; Fluorescence ; Food quality ; Gene expression ; Genes ; Hydrogen ; Hydrogen ion concentration ; Hydrogen sulfide ; Hydrogen Sulfide - metabolism ; Hydrogen Sulfide - pharmacology ; Inhibition ; Life sciences ; Lycopersicon esculentum - genetics ; Lycopersicon esculentum - metabolism ; Mammals ; Medical imaging ; Molecular Imaging ; Nitric oxide ; Nitric Oxide - metabolism ; Pharmacology ; Physiological aspects ; Plant Roots - drug effects ; Plant Roots - genetics ; Plant Roots - growth & development ; Plant Roots - metabolism ; Plants (botany) ; Regulation ; Seeds ; Single nucleotide polymorphisms ; Single-nucleotide polymorphism ; Solanum lycopersicum ; Synthesis ; Tomatoes</subject><ispartof>PloS one, 2014-02, Vol.9 (2), p.e90340</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Li et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Li et al 2014 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-2cea54e9d239eafb65d6cf6ed867ad8e2b631c09496beaf4095723e4926d3a193</citedby><cites>FETCH-LOGICAL-c692t-2cea54e9d239eafb65d6cf6ed867ad8e2b631c09496beaf4095723e4926d3a193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937356/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937356/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24587333$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Yan-Jun</creatorcontrib><creatorcontrib>Chen, Jian</creatorcontrib><creatorcontrib>Xian, Ming</creatorcontrib><creatorcontrib>Zhou, Li-Gang</creatorcontrib><creatorcontrib>Han, Fengxiang X</creatorcontrib><creatorcontrib>Gan, Li-Jun</creatorcontrib><creatorcontrib>Shi, Zhi-Qi</creatorcontrib><title>In site bioimaging of hydrogen sulfide uncovers its pivotal role in regulating nitric oxide-induced lateral root formation</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Hydrogen sulfide (H2S) is an important gasotransmitter in mammals. Despite physiological changes induced by exogenous H2S donor NaHS to plants, whether and how H2S works as a true cellular signal in plants need to be examined. A self-developed specific fluorescent probe (WSP-1) was applied to track endogenous H2S in tomato (Solanum lycopersicum) roots in site. Bioimaging combined with pharmacological and biochemical approaches were used to investigate the cross-talk among H2S, nitric oxide (NO), and Ca(2+) in regulating lateral root formation. Endogenous H2S accumulation was clearly associated with primordium initiation and lateral root emergence. NO donor SNP stimulated the generation of endogenous H2S and the expression of the gene coding for the enzyme responsible for endogenous H2S synthesis. Scavenging H2S or inhibiting H2S synthesis partially blocked SNP-induced lateral root formation and the expression of lateral root-related genes. The stimulatory effect of SNP on Ca(2+) accumulation and CaM1 (calmodulin 1) expression could be abolished by inhibiting H2S synthesis. Ca(2+) chelator or Ca(2+) channel blocker attenuated NaHS-induced lateral root formation. Our study confirmed the role of H2S as a cellular signal in plants being a mediator between NO and Ca(2+) in regulating lateral root formation.</description><subject>Accumulation</subject><subject>Biology</subject><subject>Calcium - metabolism</subject><subject>Calcium binding proteins</subject><subject>Calcium oxide</subject><subject>Calcium-binding protein</subject><subject>Calmodulin</subject><subject>Calmodulin - metabolism</subject><subject>Carbon monoxide</subject><subject>Cell cycle</subject><subject>Dose-Response Relationship, Drug</subject><subject>Engineering</subject><subject>Fluorescence</subject><subject>Food quality</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Hydrogen</subject><subject>Hydrogen ion concentration</subject><subject>Hydrogen sulfide</subject><subject>Hydrogen Sulfide - metabolism</subject><subject>Hydrogen Sulfide - pharmacology</subject><subject>Inhibition</subject><subject>Life sciences</subject><subject>Lycopersicon esculentum - genetics</subject><subject>Lycopersicon esculentum - metabolism</subject><subject>Mammals</subject><subject>Medical imaging</subject><subject>Molecular Imaging</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - metabolism</subject><subject>Pharmacology</subject><subject>Physiological aspects</subject><subject>Plant Roots - drug effects</subject><subject>Plant Roots - genetics</subject><subject>Plant Roots - growth & development</subject><subject>Plant Roots - metabolism</subject><subject>Plants (botany)</subject><subject>Regulation</subject><subject>Seeds</subject><subject>Single nucleotide polymorphisms</subject><subject>Single-nucleotide polymorphism</subject><subject>Solanum lycopersicum</subject><subject>Synthesis</subject><subject>Tomatoes</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAYhYso7jr6D0QDguBFxzRp0-ZGWBY_BhYW_LoNafK2k6GTjEk67Prrzex0lyko2Fy05H3OaXI4WfaywMuC1sX7jRu9lcNy5ywsMeaYlvhRdl5wSnJGMH188n2WPQthg3FFG8aeZmekrJqaUnqe_V5ZFEwE1BpntrI3tkeuQ-tb7V0PaTYOndGARqvcHnxAJga0M3sX5YC8GwAZizz04yDjQWtN9EYhd5NEubF6VKBRmoG_411EnfPbxDr7PHvSySHAi-m9yH58-vj98kt-df15dXlxlSvGScyJAlmVwDWhHGTXskoz1THQDaulboC0jBYK85KzNs1LzKuaUCg5YZrKFMEie3303Q0uiCm2IIoKU8waTqpErI6EdnIjdj4F4W-Fk0bcbTjfC-mjUQMIokmhoUj-Upctb5q6bGRdQ0OLolMNS14fpr-N7Ra0AhvT1Wem84k1a9G7vaCc1rQ6GLyZDLz7NUKI_zjyRPUyncrYziUztTVBiYuybupUh_QssuVfqLQ0bI1KxelM2p8J3s0EiYlwE3s5hiBW377-P3v9c86-PWHXIIe4Dm4YDz0Ic7A8gsq7EDx0D8kVWBx6f5-GOPReTL1PslenqT-I7otO_wBsswAN</recordid><startdate>20140227</startdate><enddate>20140227</enddate><creator>Li, Yan-Jun</creator><creator>Chen, Jian</creator><creator>Xian, Ming</creator><creator>Zhou, Li-Gang</creator><creator>Han, Fengxiang X</creator><creator>Gan, Li-Jun</creator><creator>Shi, Zhi-Qi</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140227</creationdate><title>In site bioimaging of hydrogen sulfide uncovers its pivotal role in regulating nitric oxide-induced lateral root formation</title><author>Li, Yan-Jun ; Chen, Jian ; Xian, Ming ; Zhou, Li-Gang ; Han, Fengxiang X ; Gan, Li-Jun ; Shi, Zhi-Qi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-2cea54e9d239eafb65d6cf6ed867ad8e2b631c09496beaf4095723e4926d3a193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Accumulation</topic><topic>Biology</topic><topic>Calcium - metabolism</topic><topic>Calcium binding proteins</topic><topic>Calcium oxide</topic><topic>Calcium-binding protein</topic><topic>Calmodulin</topic><topic>Calmodulin - metabolism</topic><topic>Carbon monoxide</topic><topic>Cell cycle</topic><topic>Dose-Response Relationship, Drug</topic><topic>Engineering</topic><topic>Fluorescence</topic><topic>Food quality</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Hydrogen</topic><topic>Hydrogen ion concentration</topic><topic>Hydrogen sulfide</topic><topic>Hydrogen Sulfide - metabolism</topic><topic>Hydrogen Sulfide - pharmacology</topic><topic>Inhibition</topic><topic>Life sciences</topic><topic>Lycopersicon esculentum - genetics</topic><topic>Lycopersicon esculentum - metabolism</topic><topic>Mammals</topic><topic>Medical imaging</topic><topic>Molecular Imaging</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - metabolism</topic><topic>Pharmacology</topic><topic>Physiological aspects</topic><topic>Plant Roots - drug effects</topic><topic>Plant Roots - 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Despite physiological changes induced by exogenous H2S donor NaHS to plants, whether and how H2S works as a true cellular signal in plants need to be examined. A self-developed specific fluorescent probe (WSP-1) was applied to track endogenous H2S in tomato (Solanum lycopersicum) roots in site. Bioimaging combined with pharmacological and biochemical approaches were used to investigate the cross-talk among H2S, nitric oxide (NO), and Ca(2+) in regulating lateral root formation. Endogenous H2S accumulation was clearly associated with primordium initiation and lateral root emergence. NO donor SNP stimulated the generation of endogenous H2S and the expression of the gene coding for the enzyme responsible for endogenous H2S synthesis. Scavenging H2S or inhibiting H2S synthesis partially blocked SNP-induced lateral root formation and the expression of lateral root-related genes. The stimulatory effect of SNP on Ca(2+) accumulation and CaM1 (calmodulin 1) expression could be abolished by inhibiting H2S synthesis. Ca(2+) chelator or Ca(2+) channel blocker attenuated NaHS-induced lateral root formation. Our study confirmed the role of H2S as a cellular signal in plants being a mediator between NO and Ca(2+) in regulating lateral root formation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24587333</pmid><doi>10.1371/journal.pone.0090340</doi><tpages>e90340</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Accumulation Biology Calcium - metabolism Calcium binding proteins Calcium oxide Calcium-binding protein Calmodulin Calmodulin - metabolism Carbon monoxide Cell cycle Dose-Response Relationship, Drug Engineering Fluorescence Food quality Gene expression Genes Hydrogen Hydrogen ion concentration Hydrogen sulfide Hydrogen Sulfide - metabolism Hydrogen Sulfide - pharmacology Inhibition Life sciences Lycopersicon esculentum - genetics Lycopersicon esculentum - metabolism Mammals Medical imaging Molecular Imaging Nitric oxide Nitric Oxide - metabolism Pharmacology Physiological aspects Plant Roots - drug effects Plant Roots - genetics Plant Roots - growth & development Plant Roots - metabolism Plants (botany) Regulation Seeds Single nucleotide polymorphisms Single-nucleotide polymorphism Solanum lycopersicum Synthesis Tomatoes |
| title | In site bioimaging of hydrogen sulfide uncovers its pivotal role in regulating nitric oxide-induced lateral root formation |
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