Expression of the fatty acid receptor GPR120 in the gut of diet-induced-obese rats and its role in GLP-1 secretion
Stimulation of the G protein coupled receptor GPR120 has been shown to have anti-inflammatory and insulin-sensitizing effects, to promote glucagon like peptide-1 (GLP-1) secretion, and to play a key role in sensing dietary fat and control energy balance. In a search for differentially expressed gene...
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| description | Stimulation of the G protein coupled receptor GPR120 has been shown to have anti-inflammatory and insulin-sensitizing effects, to promote glucagon like peptide-1 (GLP-1) secretion, and to play a key role in sensing dietary fat and control energy balance. In a search for differentially expressed genes potentially involved in food intake and body-weight regulation we identified GPR120 to be differentially regulated in the intestine of selectively bred diet induced obese (DIO) and diet resistant (DR) rats. Subsequently we investigated the effect of GPR120 receptor stimulation with the long chain fatty acid alpha linolenic acid (ALA) on GLP-1 secretion in rats. Independent of diet (high or low fat), GPR120 expression showed a two-fold increase in the intestine of DIO compared to DR rats. In situ hybridization revealed a broad expression of GPR120 in the gut mucosa in both intestinal epithelial and endocrine cells. Using double in situ hybridization GPR120 mRNA did not appear to be enriched in preproglucagon expressing L-cells. In line with the anatomical data, ALA administration did not increase circulating GLP-1 levels. Our data shows a widespread expression of GPR120 in the gut epithelium and can not confirm a major role for GPR120 in the regulation of GLP-1 secretion. The broad expression of GPR120 in the gut epithelium supports reports indicating a putative role of GPR120 as a sensor of dietary fat. |
| doi_str_mv | 10.1371/journal.pone.0088227 |
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In a search for differentially expressed genes potentially involved in food intake and body-weight regulation we identified GPR120 to be differentially regulated in the intestine of selectively bred diet induced obese (DIO) and diet resistant (DR) rats. Subsequently we investigated the effect of GPR120 receptor stimulation with the long chain fatty acid alpha linolenic acid (ALA) on GLP-1 secretion in rats. Independent of diet (high or low fat), GPR120 expression showed a two-fold increase in the intestine of DIO compared to DR rats. In situ hybridization revealed a broad expression of GPR120 in the gut mucosa in both intestinal epithelial and endocrine cells. Using double in situ hybridization GPR120 mRNA did not appear to be enriched in preproglucagon expressing L-cells. In line with the anatomical data, ALA administration did not increase circulating GLP-1 levels. Our data shows a widespread expression of GPR120 in the gut epithelium and can not confirm a major role for GPR120 in the regulation of GLP-1 secretion. The broad expression of GPR120 in the gut epithelium supports reports indicating a putative role of GPR120 as a sensor of dietary fat.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0088227</identifier><identifier>PMID: 24520357</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Administration, Oral ; Animals ; Biology ; Caprylates - administration & dosage ; Caprylates - pharmacology ; Diabetes ; Diet ; Dietary fat ; Digestive system ; Digestive tract ; Energy balance ; Epithelium ; Fatty acids ; Food intake ; Gastrointestinal tract ; Gastrointestinal Tract - drug effects ; Gastrointestinal Tract - metabolism ; Gene expression ; Genes ; Glucagon ; Glucagon-Like Peptide 1 - blood ; Glucagon-Like Peptide 1 - metabolism ; Glucose - administration & dosage ; Glucose - pharmacology ; Histology ; Hybridization ; Inflammation ; Insulin ; Insulin - blood ; Insulin resistance ; Intestine ; Laboratory animals ; Linoleic Acid - administration & dosage ; Linoleic Acid - pharmacology ; Linolenic acid ; Linolenic acids ; Lipids ; Low fat ; Male ; Medicine ; mRNA ; Mucosa ; Multiplex Polymerase Chain Reaction ; Obesity ; Obesity - blood ; Obesity - metabolism ; Oils & fats ; Proglucagon - metabolism ; Protein Transport - drug effects ; Proteins ; Rats ; Rats, Sprague-Dawley ; Rats, Wistar ; Receptors, G-Protein-Coupled - metabolism ; RNA ; Rodents ; Secretion ; Sensitizing ; Sensors ; Stimulation</subject><ispartof>PloS one, 2014-02, Vol.9 (2), p.e88227-e88227</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Paulsen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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In a search for differentially expressed genes potentially involved in food intake and body-weight regulation we identified GPR120 to be differentially regulated in the intestine of selectively bred diet induced obese (DIO) and diet resistant (DR) rats. Subsequently we investigated the effect of GPR120 receptor stimulation with the long chain fatty acid alpha linolenic acid (ALA) on GLP-1 secretion in rats. Independent of diet (high or low fat), GPR120 expression showed a two-fold increase in the intestine of DIO compared to DR rats. In situ hybridization revealed a broad expression of GPR120 in the gut mucosa in both intestinal epithelial and endocrine cells. Using double in situ hybridization GPR120 mRNA did not appear to be enriched in preproglucagon expressing L-cells. In line with the anatomical data, ALA administration did not increase circulating GLP-1 levels. Our data shows a widespread expression of GPR120 in the gut epithelium and can not confirm a major role for GPR120 in the regulation of GLP-1 secretion. The broad expression of GPR120 in the gut epithelium supports reports indicating a putative role of GPR120 as a sensor of dietary fat.</description><subject>Administration, Oral</subject><subject>Animals</subject><subject>Biology</subject><subject>Caprylates - administration & dosage</subject><subject>Caprylates - pharmacology</subject><subject>Diabetes</subject><subject>Diet</subject><subject>Dietary fat</subject><subject>Digestive system</subject><subject>Digestive tract</subject><subject>Energy balance</subject><subject>Epithelium</subject><subject>Fatty acids</subject><subject>Food intake</subject><subject>Gastrointestinal tract</subject><subject>Gastrointestinal Tract - drug effects</subject><subject>Gastrointestinal Tract - metabolism</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Glucagon</subject><subject>Glucagon-Like Peptide 1 - blood</subject><subject>Glucagon-Like Peptide 1 - metabolism</subject><subject>Glucose - administration & dosage</subject><subject>Glucose - 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In a search for differentially expressed genes potentially involved in food intake and body-weight regulation we identified GPR120 to be differentially regulated in the intestine of selectively bred diet induced obese (DIO) and diet resistant (DR) rats. Subsequently we investigated the effect of GPR120 receptor stimulation with the long chain fatty acid alpha linolenic acid (ALA) on GLP-1 secretion in rats. Independent of diet (high or low fat), GPR120 expression showed a two-fold increase in the intestine of DIO compared to DR rats. In situ hybridization revealed a broad expression of GPR120 in the gut mucosa in both intestinal epithelial and endocrine cells. Using double in situ hybridization GPR120 mRNA did not appear to be enriched in preproglucagon expressing L-cells. In line with the anatomical data, ALA administration did not increase circulating GLP-1 levels. Our data shows a widespread expression of GPR120 in the gut epithelium and can not confirm a major role for GPR120 in the regulation of GLP-1 secretion. The broad expression of GPR120 in the gut epithelium supports reports indicating a putative role of GPR120 as a sensor of dietary fat.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24520357</pmid><doi>10.1371/journal.pone.0088227</doi><tpages>e88227</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-02, Vol.9 (2), p.e88227-e88227 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1497948449 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Administration, Oral Animals Biology Caprylates - administration & dosage Caprylates - pharmacology Diabetes Diet Dietary fat Digestive system Digestive tract Energy balance Epithelium Fatty acids Food intake Gastrointestinal tract Gastrointestinal Tract - drug effects Gastrointestinal Tract - metabolism Gene expression Genes Glucagon Glucagon-Like Peptide 1 - blood Glucagon-Like Peptide 1 - metabolism Glucose - administration & dosage Glucose - pharmacology Histology Hybridization Inflammation Insulin Insulin - blood Insulin resistance Intestine Laboratory animals Linoleic Acid - administration & dosage Linoleic Acid - pharmacology Linolenic acid Linolenic acids Lipids Low fat Male Medicine mRNA Mucosa Multiplex Polymerase Chain Reaction Obesity Obesity - blood Obesity - metabolism Oils & fats Proglucagon - metabolism Protein Transport - drug effects Proteins Rats Rats, Sprague-Dawley Rats, Wistar Receptors, G-Protein-Coupled - metabolism RNA Rodents Secretion Sensitizing Sensors Stimulation |
| title | Expression of the fatty acid receptor GPR120 in the gut of diet-induced-obese rats and its role in GLP-1 secretion |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T00%3A37%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20the%20fatty%20acid%20receptor%20GPR120%20in%20the%20gut%20of%20diet-induced-obese%20rats%20and%20its%20role%20in%20GLP-1%20secretion&rft.jtitle=PloS%20one&rft.au=Paulsen,%20Sarah%20Juel&rft.date=2014-02-10&rft.volume=9&rft.issue=2&rft.spage=e88227&rft.epage=e88227&rft.pages=e88227-e88227&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0088227&rft_dat=%3Cgale_plos_%3EA478816773%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1497948449&rft_id=info:pmid/24520357&rft_galeid=A478816773&rft_doaj_id=oai_doaj_org_article_27b213cc4d2845e2b6f2ee9334ff1d51&rfr_iscdi=true |