Expression of miR-29c, miR-93, and miR-429 as potential biomarkers for detection of early stage non-small lung cancer
Altered expression of miRNA expression contributes to human carcinogenesis. This study was designed to detect aberrant miRNA expressions as a potential biomarker for early detection and prognosis prediction of non-small cell lung cancer (NSCLC). miRNA array was used to profile differentially express...
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| Veröffentlicht in: | PloS one 2014-02, Vol.9 (2), p.e87780 |
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| creator | Zhu, Wangyu He, Jianying Chen, Dongdong Zhang, Bingjie Xu, Liyun Ma, Haijie Liu, Xiaoguang Zhang, Yongkui Le, Hanbo |
| description | Altered expression of miRNA expression contributes to human carcinogenesis. This study was designed to detect aberrant miRNA expressions as a potential biomarker for early detection and prognosis prediction of non-small cell lung cancer (NSCLC).
miRNA array was used to profile differentially expressed miRNAs and Taqman-based quantitative RT-PCR assays were used to analyze levels of miR-29c, miR-93, and miR-429 expression in NSCLC tissue samples, corresponding normal tissue samples, and serum samples from 70 NSCLC patients as well as in serum samples from 48 healthy controls.
Levels of miR-29c and miR-93 expression were upregulated in NSCLC tissues, while serum levels of miR-29c were also upregulated, but levels of serum miR-429 were decreased in NSCLC. Moreover, the levels of miR-429 expression in NSCLC tissues were associated with those in serum samples. Receiver operating characteristic (ROC) curve analysis showed that at the optimal cut-off point, the areas under the ROC curve for serum levels of miR-29c and miR-429 were 0.723 and 0.727, respectively, levels which are higher than that of carcinoma embryonic antigen (0.534) in diagnosis of stage I NSCLC. In addition, serum levels of miR-429 were associated with poor overall survival of NSCLC patients. Both univariate and multivariate analyses showed that serum miR-429 level was an independent prognostic predictor for NSCLC.
The results of the current study suggest that detection of serum miR-29c and miR-429 expression should be further evaluated as a novel, non-invasive biomarker for early stage NSCLC. |
| doi_str_mv | 10.1371/journal.pone.0087780 |
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miRNA array was used to profile differentially expressed miRNAs and Taqman-based quantitative RT-PCR assays were used to analyze levels of miR-29c, miR-93, and miR-429 expression in NSCLC tissue samples, corresponding normal tissue samples, and serum samples from 70 NSCLC patients as well as in serum samples from 48 healthy controls.
Levels of miR-29c and miR-93 expression were upregulated in NSCLC tissues, while serum levels of miR-29c were also upregulated, but levels of serum miR-429 were decreased in NSCLC. Moreover, the levels of miR-429 expression in NSCLC tissues were associated with those in serum samples. Receiver operating characteristic (ROC) curve analysis showed that at the optimal cut-off point, the areas under the ROC curve for serum levels of miR-29c and miR-429 were 0.723 and 0.727, respectively, levels which are higher than that of carcinoma embryonic antigen (0.534) in diagnosis of stage I NSCLC. In addition, serum levels of miR-429 were associated with poor overall survival of NSCLC patients. Both univariate and multivariate analyses showed that serum miR-429 level was an independent prognostic predictor for NSCLC.
The results of the current study suggest that detection of serum miR-29c and miR-429 expression should be further evaluated as a novel, non-invasive biomarker for early stage NSCLC.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0087780</identifier><identifier>PMID: 24523873</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aberration ; Adult ; Aged ; Analysis ; Area Under Curve ; Bioindicators ; Biology ; Biomarkers ; Biomarkers, Tumor - metabolism ; Cancer ; Cancer therapies ; Carcinogenesis ; Carcinogens ; Carcinoma, Non-Small-Cell Lung - genetics ; Carcinoma, Non-Small-Cell Lung - metabolism ; Case-Control Studies ; Cell cycle ; Cohort Studies ; Development and progression ; Embryos ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Laboratories ; Lung cancer ; Lung diseases ; Lung Neoplasms - genetics ; Lung Neoplasms - metabolism ; Male ; Medical diagnosis ; Medical prognosis ; Medicine ; Metastasis ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; miRNA ; Non-small cell lung cancer ; Non-small cell lung carcinoma ; Patients ; Polymerase chain reaction ; Prognosis ; Prostate ; ROC Curve ; Serum levels ; Studies ; Surgery ; Thoracic surgery ; Tissues ; Tumors</subject><ispartof>PloS one, 2014-02, Vol.9 (2), p.e87780</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 zhu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 zhu et al 2014 zhu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-4650a5153ff10efe2cfe35a47daa496aa23f236ab5934c0bea7fdb878457c59a3</citedby><cites>FETCH-LOGICAL-c758t-4650a5153ff10efe2cfe35a47daa496aa23f236ab5934c0bea7fdb878457c59a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921142/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921142/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24523873$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Wangyu</creatorcontrib><creatorcontrib>He, Jianying</creatorcontrib><creatorcontrib>Chen, Dongdong</creatorcontrib><creatorcontrib>Zhang, Bingjie</creatorcontrib><creatorcontrib>Xu, Liyun</creatorcontrib><creatorcontrib>Ma, Haijie</creatorcontrib><creatorcontrib>Liu, Xiaoguang</creatorcontrib><creatorcontrib>Zhang, Yongkui</creatorcontrib><creatorcontrib>Le, Hanbo</creatorcontrib><title>Expression of miR-29c, miR-93, and miR-429 as potential biomarkers for detection of early stage non-small lung cancer</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Altered expression of miRNA expression contributes to human carcinogenesis. This study was designed to detect aberrant miRNA expressions as a potential biomarker for early detection and prognosis prediction of non-small cell lung cancer (NSCLC).
miRNA array was used to profile differentially expressed miRNAs and Taqman-based quantitative RT-PCR assays were used to analyze levels of miR-29c, miR-93, and miR-429 expression in NSCLC tissue samples, corresponding normal tissue samples, and serum samples from 70 NSCLC patients as well as in serum samples from 48 healthy controls.
Levels of miR-29c and miR-93 expression were upregulated in NSCLC tissues, while serum levels of miR-29c were also upregulated, but levels of serum miR-429 were decreased in NSCLC. Moreover, the levels of miR-429 expression in NSCLC tissues were associated with those in serum samples. Receiver operating characteristic (ROC) curve analysis showed that at the optimal cut-off point, the areas under the ROC curve for serum levels of miR-29c and miR-429 were 0.723 and 0.727, respectively, levels which are higher than that of carcinoma embryonic antigen (0.534) in diagnosis of stage I NSCLC. In addition, serum levels of miR-429 were associated with poor overall survival of NSCLC patients. Both univariate and multivariate analyses showed that serum miR-429 level was an independent prognostic predictor for NSCLC.
The results of the current study suggest that detection of serum miR-29c and miR-429 expression should be further evaluated as a novel, non-invasive biomarker for early stage NSCLC.</description><subject>Aberration</subject><subject>Adult</subject><subject>Aged</subject><subject>Analysis</subject><subject>Area Under Curve</subject><subject>Bioindicators</subject><subject>Biology</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Carcinogenesis</subject><subject>Carcinogens</subject><subject>Carcinoma, Non-Small-Cell Lung - genetics</subject><subject>Carcinoma, Non-Small-Cell Lung - metabolism</subject><subject>Case-Control Studies</subject><subject>Cell cycle</subject><subject>Cohort Studies</subject><subject>Development and progression</subject><subject>Embryos</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Laboratories</subject><subject>Lung cancer</subject><subject>Lung diseases</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - metabolism</subject><subject>Male</subject><subject>Medical diagnosis</subject><subject>Medical prognosis</subject><subject>Medicine</subject><subject>Metastasis</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>miRNA</subject><subject>Non-small cell lung cancer</subject><subject>Non-small cell lung carcinoma</subject><subject>Patients</subject><subject>Polymerase chain reaction</subject><subject>Prognosis</subject><subject>Prostate</subject><subject>ROC Curve</subject><subject>Serum levels</subject><subject>Studies</subject><subject>Surgery</subject><subject>Thoracic surgery</subject><subject>Tissues</subject><subject>Tumors</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl1rFDEUhgdRbK3-A9EBQRA6azJJJsmNUErVhUKhftyGM_mYnXV2sk1mpP33prvTsgMKkoscTp7zJnl5s-w1RgtMOP649mPooVtsfW8XCAnOBXqSHWNJyqIqEXl6UB9lL2JcI8SIqKrn2VFJWUkEJ8fZeHG7DTbG1ve5d_mmvS5KqU93hSSnOfRmV9NS5hDzrR9sP7TQ5XXrNxB-2RBz50Nu7GD1MKlYCN1dHgdobN77vogb6Lq8G_sm19BrG15mzxx00b6a9pPsx-eL7-dfi8urL8vzs8tCcyaGglYMAcOMOIeRdbbUzhIGlBsAKiuAkriSVFAzSahGtQXuTC24oIxrJoGcZG_3utvORzU5FhWmkmNBsZCJWO4J42GttqFNn7pTHlq1a_jQKAhDqzurRGUEqxGmBhFqOZbOcEclllQbXFKTtD5Nt431xhqdnArQzUTnJ327Uo3_rYgsMaZlEng3CQR_M9o4_OPJE9VAelXbO5_E9KaNWp1RLgQmgqFELf5CpWXsptUpM65N_dnAh9lAYgZ7OzQwxqiW367_n736OWffH7ArC92wir4b78MS5yDdgzr4GIN1j85hpO4j_-CGuo-8miKfxt4cuv449JBx8gdSN_q4</recordid><startdate>20140211</startdate><enddate>20140211</enddate><creator>Zhu, Wangyu</creator><creator>He, Jianying</creator><creator>Chen, Dongdong</creator><creator>Zhang, Bingjie</creator><creator>Xu, Liyun</creator><creator>Ma, Haijie</creator><creator>Liu, Xiaoguang</creator><creator>Zhang, Yongkui</creator><creator>Le, Hanbo</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140211</creationdate><title>Expression of miR-29c, miR-93, and miR-429 as potential biomarkers for detection of early stage non-small lung cancer</title><author>Zhu, Wangyu ; He, Jianying ; Chen, Dongdong ; Zhang, Bingjie ; Xu, Liyun ; Ma, Haijie ; Liu, Xiaoguang ; Zhang, Yongkui ; Le, Hanbo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-4650a5153ff10efe2cfe35a47daa496aa23f236ab5934c0bea7fdb878457c59a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aberration</topic><topic>Adult</topic><topic>Aged</topic><topic>Analysis</topic><topic>Area Under Curve</topic><topic>Bioindicators</topic><topic>Biology</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - 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This study was designed to detect aberrant miRNA expressions as a potential biomarker for early detection and prognosis prediction of non-small cell lung cancer (NSCLC).
miRNA array was used to profile differentially expressed miRNAs and Taqman-based quantitative RT-PCR assays were used to analyze levels of miR-29c, miR-93, and miR-429 expression in NSCLC tissue samples, corresponding normal tissue samples, and serum samples from 70 NSCLC patients as well as in serum samples from 48 healthy controls.
Levels of miR-29c and miR-93 expression were upregulated in NSCLC tissues, while serum levels of miR-29c were also upregulated, but levels of serum miR-429 were decreased in NSCLC. Moreover, the levels of miR-429 expression in NSCLC tissues were associated with those in serum samples. Receiver operating characteristic (ROC) curve analysis showed that at the optimal cut-off point, the areas under the ROC curve for serum levels of miR-29c and miR-429 were 0.723 and 0.727, respectively, levels which are higher than that of carcinoma embryonic antigen (0.534) in diagnosis of stage I NSCLC. In addition, serum levels of miR-429 were associated with poor overall survival of NSCLC patients. Both univariate and multivariate analyses showed that serum miR-429 level was an independent prognostic predictor for NSCLC.
The results of the current study suggest that detection of serum miR-29c and miR-429 expression should be further evaluated as a novel, non-invasive biomarker for early stage NSCLC.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24523873</pmid><doi>10.1371/journal.pone.0087780</doi><tpages>e87780</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2014-02, Vol.9 (2), p.e87780 |
| issn | 1932-6203 1932-6203 |
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| recordid | cdi_plos_journals_1497184189 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Aberration Adult Aged Analysis Area Under Curve Bioindicators Biology Biomarkers Biomarkers, Tumor - metabolism Cancer Cancer therapies Carcinogenesis Carcinogens Carcinoma, Non-Small-Cell Lung - genetics Carcinoma, Non-Small-Cell Lung - metabolism Case-Control Studies Cell cycle Cohort Studies Development and progression Embryos Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Laboratories Lung cancer Lung diseases Lung Neoplasms - genetics Lung Neoplasms - metabolism Male Medical diagnosis Medical prognosis Medicine Metastasis MicroRNA MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism Middle Aged miRNA Non-small cell lung cancer Non-small cell lung carcinoma Patients Polymerase chain reaction Prognosis Prostate ROC Curve Serum levels Studies Surgery Thoracic surgery Tissues Tumors |
| title | Expression of miR-29c, miR-93, and miR-429 as potential biomarkers for detection of early stage non-small lung cancer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T14%3A29%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20miR-29c,%20miR-93,%20and%20miR-429%20as%20potential%20biomarkers%20for%20detection%20of%20early%20stage%20non-small%20lung%20cancer&rft.jtitle=PloS%20one&rft.au=Zhu,%20Wangyu&rft.date=2014-02-11&rft.volume=9&rft.issue=2&rft.spage=e87780&rft.pages=e87780-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0087780&rft_dat=%3Cgale_plos_%3EA478813850%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1497184189&rft_id=info:pmid/24523873&rft_galeid=A478813850&rft_doaj_id=oai_doaj_org_article_86d85b014d034e719fd7f49194cd124d&rfr_iscdi=true |