Autophagy is involved in the cardioprotection effect of remote limb ischemic postconditioning on myocardial ischemia/reperfusion injury in normal mice, but not diabetic mice
Recent animal study and clinical trial data suggested that remote limb ischemic postconditioning (RIPostC) can invoke potent cardioprotection. However, during ischemia reperfusion injury (IR), the effect and mechanism of RIPostC on myocardium in subjects with or without diabetes mellitus (DM) are po...
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| creator | Han, Zhihua Cao, Jiatian Song, Dongqiang Tian, Lei Chen, Kan Wang, Yue Gao, Lin Yin, Zhaofang Fan, Yuqi Wang, Changqian |
| description | Recent animal study and clinical trial data suggested that remote limb ischemic postconditioning (RIPostC) can invoke potent cardioprotection. However, during ischemia reperfusion injury (IR), the effect and mechanism of RIPostC on myocardium in subjects with or without diabetes mellitus (DM) are poorly understood. Autophagy plays a crucial role in alleviating myocardial IR injury. The aim of this study was to determine the effect of RIPostC on mice myocardial IR injury model with or without DM, and investigate the role of autophagy in this process.
Streptozocin (STZ) induced DM mice model and myocardial IR model were established. Using a noninvasive technique, RIPostC was induced in normal mice (ND) and DM mice by three cycles of ischemia (5 min) and reperfusion (5 min) in the left hindlimb. In ND group, RIPostC significantly reduced infarct size (32.6±3.0% in ND-RIPostC vs. 50.6±2.4% in ND-IR, p |
| doi_str_mv | 10.1371/journal.pone.0086838 |
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Streptozocin (STZ) induced DM mice model and myocardial IR model were established. Using a noninvasive technique, RIPostC was induced in normal mice (ND) and DM mice by three cycles of ischemia (5 min) and reperfusion (5 min) in the left hindlimb. In ND group, RIPostC significantly reduced infarct size (32.6±3.0% in ND-RIPostC vs. 50.6±2.4% in ND-IR, p<0.05) and improved cardiac ejection fraction (49.70±3.46% in ND-RIPostC vs. 31.30±3.95% in ND-IR, p<0.05). However, in DM group, no RIPostC mediated cardioprotetion effect was observed. To analyze the role of autophagy, western blot and immunohistochemistry was performed. Our data showed that a decreased sequestosome 1 (SQSTM1/p62) level, an increased Beclin-1 level, and higher ratio of LC3-II/LC3-I were observed in ND RIPostC group, but not DM RIPostC group.
The current study suggested that RIPostC exerts cardioprotection effect on IR in normal mice, but not DM mice, and this difference is via, at least in part, the up-regulation of autophagy.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0086838</identifier><identifier>PMID: 24466263</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Autophagy ; Biology ; Blotting, Western ; Cardiology ; Cardiotonic Agents ; Cell death ; Clinical trials ; Diabetes ; Diabetes mellitus ; Diabetes Mellitus, Experimental - mortality ; Diabetes Mellitus, Experimental - pathology ; Disease Models, Animal ; Echocardiography ; Glucose ; Heart attacks ; Heart diseases ; Hindlimb - blood supply ; Hospitals ; Immunoenzyme Techniques ; Immunohistochemistry ; Injuries ; Ischemia ; Ischemic Preconditioning, Myocardial ; Kinases ; Laboratory animals ; Male ; Medical research ; Medical schools ; Medicine ; Mice ; Mice, Inbred C57BL ; Mortality ; Myocardial ischemia ; Myocardial Reperfusion Injury - mortality ; Myocardial Reperfusion Injury - pathology ; Myocardial Reperfusion Injury - prevention & control ; Myocardium ; Phagocytosis ; Proteins ; Reperfusion ; Rodents ; Streptozocin ; Survival Rate ; Veins & arteries ; Veterinary Science</subject><ispartof>PloS one, 2014-01, Vol.9 (1), p.e86838-e86838</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Han et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Han et al 2014 Han et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-a1c9cea09e66e11f808f78d4c05505767262bf7be4f26f5ed933cb2317c515763</citedby><cites>FETCH-LOGICAL-c692t-a1c9cea09e66e11f808f78d4c05505767262bf7be4f26f5ed933cb2317c515763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900658/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900658/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24466263$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mohanraj, Rajesh</contributor><creatorcontrib>Han, Zhihua</creatorcontrib><creatorcontrib>Cao, Jiatian</creatorcontrib><creatorcontrib>Song, Dongqiang</creatorcontrib><creatorcontrib>Tian, Lei</creatorcontrib><creatorcontrib>Chen, Kan</creatorcontrib><creatorcontrib>Wang, Yue</creatorcontrib><creatorcontrib>Gao, Lin</creatorcontrib><creatorcontrib>Yin, Zhaofang</creatorcontrib><creatorcontrib>Fan, Yuqi</creatorcontrib><creatorcontrib>Wang, Changqian</creatorcontrib><title>Autophagy is involved in the cardioprotection effect of remote limb ischemic postconditioning on myocardial ischemia/reperfusion injury in normal mice, but not diabetic mice</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Recent animal study and clinical trial data suggested that remote limb ischemic postconditioning (RIPostC) can invoke potent cardioprotection. However, during ischemia reperfusion injury (IR), the effect and mechanism of RIPostC on myocardium in subjects with or without diabetes mellitus (DM) are poorly understood. Autophagy plays a crucial role in alleviating myocardial IR injury. The aim of this study was to determine the effect of RIPostC on mice myocardial IR injury model with or without DM, and investigate the role of autophagy in this process.
Streptozocin (STZ) induced DM mice model and myocardial IR model were established. Using a noninvasive technique, RIPostC was induced in normal mice (ND) and DM mice by three cycles of ischemia (5 min) and reperfusion (5 min) in the left hindlimb. In ND group, RIPostC significantly reduced infarct size (32.6±3.0% in ND-RIPostC vs. 50.6±2.4% in ND-IR, p<0.05) and improved cardiac ejection fraction (49.70±3.46% in ND-RIPostC vs. 31.30±3.95% in ND-IR, p<0.05). However, in DM group, no RIPostC mediated cardioprotetion effect was observed. To analyze the role of autophagy, western blot and immunohistochemistry was performed. Our data showed that a decreased sequestosome 1 (SQSTM1/p62) level, an increased Beclin-1 level, and higher ratio of LC3-II/LC3-I were observed in ND RIPostC group, but not DM RIPostC group.
The current study suggested that RIPostC exerts cardioprotection effect on IR in normal mice, but not DM mice, and this difference is via, at least in part, the up-regulation of autophagy.</description><subject>Analysis</subject><subject>Animals</subject><subject>Autophagy</subject><subject>Biology</subject><subject>Blotting, Western</subject><subject>Cardiology</subject><subject>Cardiotonic Agents</subject><subject>Cell death</subject><subject>Clinical trials</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes Mellitus, Experimental - mortality</subject><subject>Diabetes Mellitus, Experimental - pathology</subject><subject>Disease Models, Animal</subject><subject>Echocardiography</subject><subject>Glucose</subject><subject>Heart attacks</subject><subject>Heart diseases</subject><subject>Hindlimb - blood supply</subject><subject>Hospitals</subject><subject>Immunoenzyme Techniques</subject><subject>Immunohistochemistry</subject><subject>Injuries</subject><subject>Ischemia</subject><subject>Ischemic Preconditioning, Myocardial</subject><subject>Kinases</subject><subject>Laboratory animals</subject><subject>Male</subject><subject>Medical research</subject><subject>Medical schools</subject><subject>Medicine</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mortality</subject><subject>Myocardial ischemia</subject><subject>Myocardial Reperfusion Injury - mortality</subject><subject>Myocardial Reperfusion Injury - pathology</subject><subject>Myocardial Reperfusion Injury - prevention & control</subject><subject>Myocardium</subject><subject>Phagocytosis</subject><subject>Proteins</subject><subject>Reperfusion</subject><subject>Rodents</subject><subject>Streptozocin</subject><subject>Survival Rate</subject><subject>Veins & arteries</subject><subject>Veterinary Science</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tq3DAQhk1padK0b1BaQ6G00N3oYEv2TWEJPSwEAj3dClke7WqxLUeSQ_eh-o6V9xDWJRfFFxKj7_9nNNYkyUuM5phyfLmxg-tkM-9tB3OEClbQ4lFyjktKZowg-vhkf5Y8836DUE4Lxp4mZyTLGCOMnid_FkOw_VqutqnxqenubHMHddykYQ2pkq42tnc2gArGdiloHXep1amDNkbTxrRVVKo1tEalvfVB2a42I2y6VRol7dbubGRz5OSlgx6cHvxoabrN4LZjxs66NlLRCD6k1RBiIKRRWEGI3mP4efJEy8bDi8N6kfz8_OnH1dfZ9c2X5dXieqZYScJMYlUqkKgExgBjXaBC86LOFMpzlHPGCSOV5hVkmjCdQ11SqipCMVc5juf0Inm99-0b68Wh017grMSUUI5GYrknais3onemlW4rrDRiF7BuJaSLZTcgqCyRwpLkuawyxKsCeFZVNaOc8KKqIXp9PGQbqhZqBV1wspmYTk86sxYreydoiRDLi2jw7mDg7O0APog2thqaRnZgh13dhCOel2Pdb_5BH77dgVrJeAHTaRvzqtFULDJeFDlGlEZq_gAVv3p8DPFZahPjE8H7iSAyAX6HlRy8F8vv3_6fvfk1Zd-esGuQTVh72wzjK_RTMNuDylnvHej7JmMkxqk6dkOMUyUOUxVlr05_0L3oOEb0L8b4IKU</recordid><startdate>20140123</startdate><enddate>20140123</enddate><creator>Han, Zhihua</creator><creator>Cao, Jiatian</creator><creator>Song, Dongqiang</creator><creator>Tian, Lei</creator><creator>Chen, Kan</creator><creator>Wang, Yue</creator><creator>Gao, Lin</creator><creator>Yin, Zhaofang</creator><creator>Fan, Yuqi</creator><creator>Wang, Changqian</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140123</creationdate><title>Autophagy is involved in the cardioprotection effect of remote limb ischemic postconditioning on myocardial ischemia/reperfusion injury in normal mice, but not diabetic mice</title><author>Han, Zhihua ; Cao, Jiatian ; Song, Dongqiang ; Tian, Lei ; Chen, Kan ; Wang, Yue ; Gao, Lin ; Yin, Zhaofang ; Fan, Yuqi ; Wang, Changqian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-a1c9cea09e66e11f808f78d4c05505767262bf7be4f26f5ed933cb2317c515763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Autophagy</topic><topic>Biology</topic><topic>Blotting, Western</topic><topic>Cardiology</topic><topic>Cardiotonic Agents</topic><topic>Cell death</topic><topic>Clinical trials</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetes Mellitus, Experimental - mortality</topic><topic>Diabetes Mellitus, Experimental - pathology</topic><topic>Disease Models, Animal</topic><topic>Echocardiography</topic><topic>Glucose</topic><topic>Heart attacks</topic><topic>Heart diseases</topic><topic>Hindlimb - blood supply</topic><topic>Hospitals</topic><topic>Immunoenzyme Techniques</topic><topic>Immunohistochemistry</topic><topic>Injuries</topic><topic>Ischemia</topic><topic>Ischemic Preconditioning, Myocardial</topic><topic>Kinases</topic><topic>Laboratory animals</topic><topic>Male</topic><topic>Medical research</topic><topic>Medical schools</topic><topic>Medicine</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mortality</topic><topic>Myocardial ischemia</topic><topic>Myocardial Reperfusion Injury - mortality</topic><topic>Myocardial Reperfusion Injury - pathology</topic><topic>Myocardial Reperfusion Injury - prevention & control</topic><topic>Myocardium</topic><topic>Phagocytosis</topic><topic>Proteins</topic><topic>Reperfusion</topic><topic>Rodents</topic><topic>Streptozocin</topic><topic>Survival Rate</topic><topic>Veins & arteries</topic><topic>Veterinary Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Zhihua</creatorcontrib><creatorcontrib>Cao, Jiatian</creatorcontrib><creatorcontrib>Song, Dongqiang</creatorcontrib><creatorcontrib>Tian, Lei</creatorcontrib><creatorcontrib>Chen, Kan</creatorcontrib><creatorcontrib>Wang, Yue</creatorcontrib><creatorcontrib>Gao, Lin</creatorcontrib><creatorcontrib>Yin, Zhaofang</creatorcontrib><creatorcontrib>Fan, Yuqi</creatorcontrib><creatorcontrib>Wang, Changqian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Zhihua</au><au>Cao, Jiatian</au><au>Song, Dongqiang</au><au>Tian, Lei</au><au>Chen, Kan</au><au>Wang, Yue</au><au>Gao, Lin</au><au>Yin, Zhaofang</au><au>Fan, Yuqi</au><au>Wang, Changqian</au><au>Mohanraj, Rajesh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autophagy is involved in the cardioprotection effect of remote limb ischemic postconditioning on myocardial ischemia/reperfusion injury in normal mice, but not diabetic mice</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-01-23</date><risdate>2014</risdate><volume>9</volume><issue>1</issue><spage>e86838</spage><epage>e86838</epage><pages>e86838-e86838</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Recent animal study and clinical trial data suggested that remote limb ischemic postconditioning (RIPostC) can invoke potent cardioprotection. However, during ischemia reperfusion injury (IR), the effect and mechanism of RIPostC on myocardium in subjects with or without diabetes mellitus (DM) are poorly understood. Autophagy plays a crucial role in alleviating myocardial IR injury. The aim of this study was to determine the effect of RIPostC on mice myocardial IR injury model with or without DM, and investigate the role of autophagy in this process.
Streptozocin (STZ) induced DM mice model and myocardial IR model were established. Using a noninvasive technique, RIPostC was induced in normal mice (ND) and DM mice by three cycles of ischemia (5 min) and reperfusion (5 min) in the left hindlimb. In ND group, RIPostC significantly reduced infarct size (32.6±3.0% in ND-RIPostC vs. 50.6±2.4% in ND-IR, p<0.05) and improved cardiac ejection fraction (49.70±3.46% in ND-RIPostC vs. 31.30±3.95% in ND-IR, p<0.05). However, in DM group, no RIPostC mediated cardioprotetion effect was observed. To analyze the role of autophagy, western blot and immunohistochemistry was performed. Our data showed that a decreased sequestosome 1 (SQSTM1/p62) level, an increased Beclin-1 level, and higher ratio of LC3-II/LC3-I were observed in ND RIPostC group, but not DM RIPostC group.
The current study suggested that RIPostC exerts cardioprotection effect on IR in normal mice, but not DM mice, and this difference is via, at least in part, the up-regulation of autophagy.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24466263</pmid><doi>10.1371/journal.pone.0086838</doi><tpages>e86838</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2014-01, Vol.9 (1), p.e86838-e86838 |
| issn | 1932-6203 1932-6203 |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Autophagy Biology Blotting, Western Cardiology Cardiotonic Agents Cell death Clinical trials Diabetes Diabetes mellitus Diabetes Mellitus, Experimental - mortality Diabetes Mellitus, Experimental - pathology Disease Models, Animal Echocardiography Glucose Heart attacks Heart diseases Hindlimb - blood supply Hospitals Immunoenzyme Techniques Immunohistochemistry Injuries Ischemia Ischemic Preconditioning, Myocardial Kinases Laboratory animals Male Medical research Medical schools Medicine Mice Mice, Inbred C57BL Mortality Myocardial ischemia Myocardial Reperfusion Injury - mortality Myocardial Reperfusion Injury - pathology Myocardial Reperfusion Injury - prevention & control Myocardium Phagocytosis Proteins Reperfusion Rodents Streptozocin Survival Rate Veins & arteries Veterinary Science |
| title | Autophagy is involved in the cardioprotection effect of remote limb ischemic postconditioning on myocardial ischemia/reperfusion injury in normal mice, but not diabetic mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T09%3A40%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Autophagy%20is%20involved%20in%20the%20cardioprotection%20effect%20of%20remote%20limb%20ischemic%20postconditioning%20on%20myocardial%20ischemia/reperfusion%20injury%20in%20normal%20mice,%20but%20not%20diabetic%20mice&rft.jtitle=PloS%20one&rft.au=Han,%20Zhihua&rft.date=2014-01-23&rft.volume=9&rft.issue=1&rft.spage=e86838&rft.epage=e86838&rft.pages=e86838-e86838&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0086838&rft_dat=%3Cgale_plos_%3EA478851033%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1491323706&rft_id=info:pmid/24466263&rft_galeid=A478851033&rft_doaj_id=oai_doaj_org_article_3a90c1a255ab407b8e74bbd637278bde&rfr_iscdi=true |