Characterization and interactome study of white spot syndrome virus envelope protein VP11
White spot syndrome virus (WSSV) is a large enveloped virus. The WSSV viral particle consists of three structural layers that surround its core DNA: an outer envelope, a tegument and a nucleocapsid. Here we characterize the WSSV structural protein VP11 (WSSV394, GenBank accession number AF440570), a...
Gespeichert in:
| Veröffentlicht in: | PloS one 2014-01, Vol.9 (1), p.e85779-e85779 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e85779 |
|---|---|
| container_issue | 1 |
| container_start_page | e85779 |
| container_title | PloS one |
| container_volume | 9 |
| creator | Liu, Wang-Jing Shiung, Hui-Jui Lo, Chu-Fang Leu, Jiann-Horng Lai, Ying-Jang Lee, Tai-Lin Huang, Wei-Tung Kou, Guang-Hsiung Chang, Yun-Shiang |
| description | White spot syndrome virus (WSSV) is a large enveloped virus. The WSSV viral particle consists of three structural layers that surround its core DNA: an outer envelope, a tegument and a nucleocapsid. Here we characterize the WSSV structural protein VP11 (WSSV394, GenBank accession number AF440570), and use an interactome approach to analyze the possible associations between this protein and an array of other WSSV and host proteins. Temporal transcription analysis showed that vp11 is an early gene. Western blot hybridization of the intact viral particles and fractionation of the viral components, and immunoelectron microscopy showed that VP11 is an envelope protein. Membrane topology software predicted VP11 to be a type of transmembrane protein with a highly hydrophobic transmembrane domain at its N-terminal. Based on an immunofluorescence assay performed on VP11-transfected Sf9 cells and a trypsin digestion analysis of the virion, we conclude that, contrary to topology software prediction, the C-terminal of this protein is in fact inside the virion. Yeast two-hybrid screening combined with co-immunoprecipitation assays found that VP11 directly interacted with at least 12 other WSSV structural proteins as well as itself. An oligomerization assay further showed that VP11 could form dimers. VP11 is also the first reported WSSV structural protein to interact with the major nucleocapsid protein VP664. |
| doi_str_mv | 10.1371/journal.pone.0085779 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1490992850</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478855616</galeid><doaj_id>oai_doaj_org_article_f5a620d467924b7b97333b5f3850537c</doaj_id><sourcerecordid>A478855616</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-398f94198177cd47d0b45bcc5cac36956518acde8886dc95224e20abc17c95343</originalsourceid><addsrcrecordid>eNqNk1uLEzEUxwdR3HX1G4gOCKIPrcnk_iIsxUthYcXLgk8hk8m0KdNkNslU66c33c4ureyD5CHJye_8k3NyTlE8h2AKEYPvVn4ITnXT3jszBYATxsSD4hQKVE1oBdDDg_VJ8STGFQAEcUofFycVxpQwAE-Ln7OlCkonE-wflax3pXJNaV02ZKtfmzKmodmWvi1_LW3K296nMm5dE3aHGxuGWBq3MZ3vTdkHn4x15dUXCJ8Wj1rVRfNsnM-KHx8_fJ99nlxcfprPzi8mmooqTZDgrcBQcMiYbjBrQI1JrTXRSiMqCCWQK90YzjlttCBVhU0FVK0hyzuE0Vnxcq_bdz7KMStRQiyAEBUnIBPzPdF4tZJ9sGsVttIrK28MPiykCsnqzsiWqJyvBlMmKlyzWjCEUE1alHUIYjprvR9vG-q1abRxKajuSPT4xNmlXPiNRFywHEoWeDMKBH89mJjk2kZtuk4544ebd1eUC45JRl_9g94f3UgtVA7Autbne_VOVJ5jxjkhFNJMTe-h8mjM2upcQq3N9iOHt0cOmUnmd1qoIUY5__b1_9nLq2P29QG7NKpLy-i7YVd78RjEe1AHH2Mw7V2SIZC7DrjNhtx1gBw7ILu9OPygO6fbkkd_AZr0AC4</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1490992850</pqid></control><display><type>article</type><title>Characterization and interactome study of white spot syndrome virus envelope protein VP11</title><source>MEDLINE</source><source>Full-Text Journals in Chemistry (Open access)</source><source>Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>PubMed (Medline)</source><source>EZB Electronic Journals Library</source><creator>Liu, Wang-Jing ; Shiung, Hui-Jui ; Lo, Chu-Fang ; Leu, Jiann-Horng ; Lai, Ying-Jang ; Lee, Tai-Lin ; Huang, Wei-Tung ; Kou, Guang-Hsiung ; Chang, Yun-Shiang</creator><contributor>Söderhäll, Irene</contributor><creatorcontrib>Liu, Wang-Jing ; Shiung, Hui-Jui ; Lo, Chu-Fang ; Leu, Jiann-Horng ; Lai, Ying-Jang ; Lee, Tai-Lin ; Huang, Wei-Tung ; Kou, Guang-Hsiung ; Chang, Yun-Shiang ; Söderhäll, Irene</creatorcontrib><description>White spot syndrome virus (WSSV) is a large enveloped virus. The WSSV viral particle consists of three structural layers that surround its core DNA: an outer envelope, a tegument and a nucleocapsid. Here we characterize the WSSV structural protein VP11 (WSSV394, GenBank accession number AF440570), and use an interactome approach to analyze the possible associations between this protein and an array of other WSSV and host proteins. Temporal transcription analysis showed that vp11 is an early gene. Western blot hybridization of the intact viral particles and fractionation of the viral components, and immunoelectron microscopy showed that VP11 is an envelope protein. Membrane topology software predicted VP11 to be a type of transmembrane protein with a highly hydrophobic transmembrane domain at its N-terminal. Based on an immunofluorescence assay performed on VP11-transfected Sf9 cells and a trypsin digestion analysis of the virion, we conclude that, contrary to topology software prediction, the C-terminal of this protein is in fact inside the virion. Yeast two-hybrid screening combined with co-immunoprecipitation assays found that VP11 directly interacted with at least 12 other WSSV structural proteins as well as itself. An oligomerization assay further showed that VP11 could form dimers. VP11 is also the first reported WSSV structural protein to interact with the major nucleocapsid protein VP664.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0085779</identifier><identifier>PMID: 24465701</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agriculture ; Analysis ; Assaying ; Biology ; Biotechnology ; Deoxyribonucleic acid ; Dimers ; DNA ; Fractionation ; Gene Expression Regulation, Viral ; Genes ; Herpes viruses ; Hybridization ; Hydrophobicity ; Immunoelectron microscopy ; Immunofluorescence ; Immunoprecipitation ; Infections ; Medical research ; Medical screening ; Membrane proteins ; Microscopy ; Molecular Sequence Data ; Nucleocapsids ; Oligomerization ; Penaeus monodon ; Predictions ; Protein arrays ; Protein Binding ; Protein Multimerization ; Proteins ; Reproducibility of Results ; Shellfish ; Structural proteins ; Tegument ; Time Factors ; Topology ; Transcription ; Transcription (Genetics) ; Transcription, Genetic ; Trypsin ; Two-Hybrid System Techniques ; Viral envelope proteins ; Viral Envelope Proteins - chemistry ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - metabolism ; Viral Envelope Proteins - ultrastructure ; Virion - metabolism ; Virions ; Virology ; Viruses ; White spot syndrome ; White spot syndrome virus 1 - genetics ; White spot syndrome virus 1 - metabolism ; White spot syndrome virus 1 - ultrastructure ; Yeast</subject><ispartof>PloS one, 2014-01, Vol.9 (1), p.e85779-e85779</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Liu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Liu et al 2014 Liu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-398f94198177cd47d0b45bcc5cac36956518acde8886dc95224e20abc17c95343</citedby><cites>FETCH-LOGICAL-c692t-398f94198177cd47d0b45bcc5cac36956518acde8886dc95224e20abc17c95343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897518/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897518/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24465701$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Söderhäll, Irene</contributor><creatorcontrib>Liu, Wang-Jing</creatorcontrib><creatorcontrib>Shiung, Hui-Jui</creatorcontrib><creatorcontrib>Lo, Chu-Fang</creatorcontrib><creatorcontrib>Leu, Jiann-Horng</creatorcontrib><creatorcontrib>Lai, Ying-Jang</creatorcontrib><creatorcontrib>Lee, Tai-Lin</creatorcontrib><creatorcontrib>Huang, Wei-Tung</creatorcontrib><creatorcontrib>Kou, Guang-Hsiung</creatorcontrib><creatorcontrib>Chang, Yun-Shiang</creatorcontrib><title>Characterization and interactome study of white spot syndrome virus envelope protein VP11</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>White spot syndrome virus (WSSV) is a large enveloped virus. The WSSV viral particle consists of three structural layers that surround its core DNA: an outer envelope, a tegument and a nucleocapsid. Here we characterize the WSSV structural protein VP11 (WSSV394, GenBank accession number AF440570), and use an interactome approach to analyze the possible associations between this protein and an array of other WSSV and host proteins. Temporal transcription analysis showed that vp11 is an early gene. Western blot hybridization of the intact viral particles and fractionation of the viral components, and immunoelectron microscopy showed that VP11 is an envelope protein. Membrane topology software predicted VP11 to be a type of transmembrane protein with a highly hydrophobic transmembrane domain at its N-terminal. Based on an immunofluorescence assay performed on VP11-transfected Sf9 cells and a trypsin digestion analysis of the virion, we conclude that, contrary to topology software prediction, the C-terminal of this protein is in fact inside the virion. Yeast two-hybrid screening combined with co-immunoprecipitation assays found that VP11 directly interacted with at least 12 other WSSV structural proteins as well as itself. An oligomerization assay further showed that VP11 could form dimers. VP11 is also the first reported WSSV structural protein to interact with the major nucleocapsid protein VP664.</description><subject>Agriculture</subject><subject>Analysis</subject><subject>Assaying</subject><subject>Biology</subject><subject>Biotechnology</subject><subject>Deoxyribonucleic acid</subject><subject>Dimers</subject><subject>DNA</subject><subject>Fractionation</subject><subject>Gene Expression Regulation, Viral</subject><subject>Genes</subject><subject>Herpes viruses</subject><subject>Hybridization</subject><subject>Hydrophobicity</subject><subject>Immunoelectron microscopy</subject><subject>Immunofluorescence</subject><subject>Immunoprecipitation</subject><subject>Infections</subject><subject>Medical research</subject><subject>Medical screening</subject><subject>Membrane proteins</subject><subject>Microscopy</subject><subject>Molecular Sequence Data</subject><subject>Nucleocapsids</subject><subject>Oligomerization</subject><subject>Penaeus monodon</subject><subject>Predictions</subject><subject>Protein arrays</subject><subject>Protein Binding</subject><subject>Protein Multimerization</subject><subject>Proteins</subject><subject>Reproducibility of Results</subject><subject>Shellfish</subject><subject>Structural proteins</subject><subject>Tegument</subject><subject>Time Factors</subject><subject>Topology</subject><subject>Transcription</subject><subject>Transcription (Genetics)</subject><subject>Transcription, Genetic</subject><subject>Trypsin</subject><subject>Two-Hybrid System Techniques</subject><subject>Viral envelope proteins</subject><subject>Viral Envelope Proteins - chemistry</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - metabolism</subject><subject>Viral Envelope Proteins - ultrastructure</subject><subject>Virion - metabolism</subject><subject>Virions</subject><subject>Virology</subject><subject>Viruses</subject><subject>White spot syndrome</subject><subject>White spot syndrome virus 1 - genetics</subject><subject>White spot syndrome virus 1 - metabolism</subject><subject>White spot syndrome virus 1 - ultrastructure</subject><subject>Yeast</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1uLEzEUxwdR3HX1G4gOCKIPrcnk_iIsxUthYcXLgk8hk8m0KdNkNslU66c33c4ureyD5CHJye_8k3NyTlE8h2AKEYPvVn4ITnXT3jszBYATxsSD4hQKVE1oBdDDg_VJ8STGFQAEcUofFycVxpQwAE-Ln7OlCkonE-wflax3pXJNaV02ZKtfmzKmodmWvi1_LW3K296nMm5dE3aHGxuGWBq3MZ3vTdkHn4x15dUXCJ8Wj1rVRfNsnM-KHx8_fJ99nlxcfprPzi8mmooqTZDgrcBQcMiYbjBrQI1JrTXRSiMqCCWQK90YzjlttCBVhU0FVK0hyzuE0Vnxcq_bdz7KMStRQiyAEBUnIBPzPdF4tZJ9sGsVttIrK28MPiykCsnqzsiWqJyvBlMmKlyzWjCEUE1alHUIYjprvR9vG-q1abRxKajuSPT4xNmlXPiNRFywHEoWeDMKBH89mJjk2kZtuk4544ebd1eUC45JRl_9g94f3UgtVA7Autbne_VOVJ5jxjkhFNJMTe-h8mjM2upcQq3N9iOHt0cOmUnmd1qoIUY5__b1_9nLq2P29QG7NKpLy-i7YVd78RjEe1AHH2Mw7V2SIZC7DrjNhtx1gBw7ILu9OPygO6fbkkd_AZr0AC4</recordid><startdate>20140121</startdate><enddate>20140121</enddate><creator>Liu, Wang-Jing</creator><creator>Shiung, Hui-Jui</creator><creator>Lo, Chu-Fang</creator><creator>Leu, Jiann-Horng</creator><creator>Lai, Ying-Jang</creator><creator>Lee, Tai-Lin</creator><creator>Huang, Wei-Tung</creator><creator>Kou, Guang-Hsiung</creator><creator>Chang, Yun-Shiang</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140121</creationdate><title>Characterization and interactome study of white spot syndrome virus envelope protein VP11</title><author>Liu, Wang-Jing ; Shiung, Hui-Jui ; Lo, Chu-Fang ; Leu, Jiann-Horng ; Lai, Ying-Jang ; Lee, Tai-Lin ; Huang, Wei-Tung ; Kou, Guang-Hsiung ; Chang, Yun-Shiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-398f94198177cd47d0b45bcc5cac36956518acde8886dc95224e20abc17c95343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Agriculture</topic><topic>Analysis</topic><topic>Assaying</topic><topic>Biology</topic><topic>Biotechnology</topic><topic>Deoxyribonucleic acid</topic><topic>Dimers</topic><topic>DNA</topic><topic>Fractionation</topic><topic>Gene Expression Regulation, Viral</topic><topic>Genes</topic><topic>Herpes viruses</topic><topic>Hybridization</topic><topic>Hydrophobicity</topic><topic>Immunoelectron microscopy</topic><topic>Immunofluorescence</topic><topic>Immunoprecipitation</topic><topic>Infections</topic><topic>Medical research</topic><topic>Medical screening</topic><topic>Membrane proteins</topic><topic>Microscopy</topic><topic>Molecular Sequence Data</topic><topic>Nucleocapsids</topic><topic>Oligomerization</topic><topic>Penaeus monodon</topic><topic>Predictions</topic><topic>Protein arrays</topic><topic>Protein Binding</topic><topic>Protein Multimerization</topic><topic>Proteins</topic><topic>Reproducibility of Results</topic><topic>Shellfish</topic><topic>Structural proteins</topic><topic>Tegument</topic><topic>Time Factors</topic><topic>Topology</topic><topic>Transcription</topic><topic>Transcription (Genetics)</topic><topic>Transcription, Genetic</topic><topic>Trypsin</topic><topic>Two-Hybrid System Techniques</topic><topic>Viral envelope proteins</topic><topic>Viral Envelope Proteins - chemistry</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - metabolism</topic><topic>Viral Envelope Proteins - ultrastructure</topic><topic>Virion - metabolism</topic><topic>Virions</topic><topic>Virology</topic><topic>Viruses</topic><topic>White spot syndrome</topic><topic>White spot syndrome virus 1 - genetics</topic><topic>White spot syndrome virus 1 - metabolism</topic><topic>White spot syndrome virus 1 - ultrastructure</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Wang-Jing</creatorcontrib><creatorcontrib>Shiung, Hui-Jui</creatorcontrib><creatorcontrib>Lo, Chu-Fang</creatorcontrib><creatorcontrib>Leu, Jiann-Horng</creatorcontrib><creatorcontrib>Lai, Ying-Jang</creatorcontrib><creatorcontrib>Lee, Tai-Lin</creatorcontrib><creatorcontrib>Huang, Wei-Tung</creatorcontrib><creatorcontrib>Kou, Guang-Hsiung</creatorcontrib><creatorcontrib>Chang, Yun-Shiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Wang-Jing</au><au>Shiung, Hui-Jui</au><au>Lo, Chu-Fang</au><au>Leu, Jiann-Horng</au><au>Lai, Ying-Jang</au><au>Lee, Tai-Lin</au><au>Huang, Wei-Tung</au><au>Kou, Guang-Hsiung</au><au>Chang, Yun-Shiang</au><au>Söderhäll, Irene</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and interactome study of white spot syndrome virus envelope protein VP11</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-01-21</date><risdate>2014</risdate><volume>9</volume><issue>1</issue><spage>e85779</spage><epage>e85779</epage><pages>e85779-e85779</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>White spot syndrome virus (WSSV) is a large enveloped virus. The WSSV viral particle consists of three structural layers that surround its core DNA: an outer envelope, a tegument and a nucleocapsid. Here we characterize the WSSV structural protein VP11 (WSSV394, GenBank accession number AF440570), and use an interactome approach to analyze the possible associations between this protein and an array of other WSSV and host proteins. Temporal transcription analysis showed that vp11 is an early gene. Western blot hybridization of the intact viral particles and fractionation of the viral components, and immunoelectron microscopy showed that VP11 is an envelope protein. Membrane topology software predicted VP11 to be a type of transmembrane protein with a highly hydrophobic transmembrane domain at its N-terminal. Based on an immunofluorescence assay performed on VP11-transfected Sf9 cells and a trypsin digestion analysis of the virion, we conclude that, contrary to topology software prediction, the C-terminal of this protein is in fact inside the virion. Yeast two-hybrid screening combined with co-immunoprecipitation assays found that VP11 directly interacted with at least 12 other WSSV structural proteins as well as itself. An oligomerization assay further showed that VP11 could form dimers. VP11 is also the first reported WSSV structural protein to interact with the major nucleocapsid protein VP664.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24465701</pmid><doi>10.1371/journal.pone.0085779</doi><tpages>e85779</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-01, Vol.9 (1), p.e85779-e85779 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1490992850 |
| source | MEDLINE; Full-Text Journals in Chemistry (Open access); Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; PubMed (Medline); EZB Electronic Journals Library |
| subjects | Agriculture Analysis Assaying Biology Biotechnology Deoxyribonucleic acid Dimers DNA Fractionation Gene Expression Regulation, Viral Genes Herpes viruses Hybridization Hydrophobicity Immunoelectron microscopy Immunofluorescence Immunoprecipitation Infections Medical research Medical screening Membrane proteins Microscopy Molecular Sequence Data Nucleocapsids Oligomerization Penaeus monodon Predictions Protein arrays Protein Binding Protein Multimerization Proteins Reproducibility of Results Shellfish Structural proteins Tegument Time Factors Topology Transcription Transcription (Genetics) Transcription, Genetic Trypsin Two-Hybrid System Techniques Viral envelope proteins Viral Envelope Proteins - chemistry Viral Envelope Proteins - genetics Viral Envelope Proteins - metabolism Viral Envelope Proteins - ultrastructure Virion - metabolism Virions Virology Viruses White spot syndrome White spot syndrome virus 1 - genetics White spot syndrome virus 1 - metabolism White spot syndrome virus 1 - ultrastructure Yeast |
| title | Characterization and interactome study of white spot syndrome virus envelope protein VP11 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T17%3A21%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20and%20interactome%20study%20of%20white%20spot%20syndrome%20virus%20envelope%20protein%20VP11&rft.jtitle=PloS%20one&rft.au=Liu,%20Wang-Jing&rft.date=2014-01-21&rft.volume=9&rft.issue=1&rft.spage=e85779&rft.epage=e85779&rft.pages=e85779-e85779&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0085779&rft_dat=%3Cgale_plos_%3EA478855616%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1490992850&rft_id=info:pmid/24465701&rft_galeid=A478855616&rft_doaj_id=oai_doaj_org_article_f5a620d467924b7b97333b5f3850537c&rfr_iscdi=true |