Engineering the Saccharomyces cerevisiae β-oxidation pathway to increase medium chain fatty acid production as potential biofuel
Fatty acid-derived biofuels and biochemicals can be produced in microbes using β-oxidation pathway engineering. In this study, the β-oxidation pathway of Saccharomyces cerevisiae was engineered to accumulate a higher ratio of medium chain fatty acids (MCFAs) when cells were grown on fatty acid-rich...
Gespeichert in:
| Veröffentlicht in: | PloS one 2014, Vol.9 (1), p.e84853-e84853 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e84853 |
|---|---|
| container_issue | 1 |
| container_start_page | e84853 |
| container_title | PloS one |
| container_volume | 9 |
| creator | Chen, Liwei Zhang, Jianhua Chen, Wei Ning |
| description | Fatty acid-derived biofuels and biochemicals can be produced in microbes using β-oxidation pathway engineering. In this study, the β-oxidation pathway of Saccharomyces cerevisiae was engineered to accumulate a higher ratio of medium chain fatty acids (MCFAs) when cells were grown on fatty acid-rich feedstock. For this purpose, the haploid deletion strain Δpox1 was obtained, in which the sole acyl-CoA oxidase encoded by POX1 was deleted. Next, the POX2 gene from Yarrowia lipolytica, which encodes an acyl-CoA oxidase with a preference for long chain acyl-CoAs, was expressed in the Δpox1 strain. The resulting Δpox1 [pox2+] strain exhibited a growth defect because the β-oxidation pathway was blocked in peroxisomes. To unblock the β-oxidation pathway, the gene CROT, which encodes carnitine O-octanoyltransferase, was expressed in the Δpox1 [pox2+] strain to transport the accumulated medium chain acyl-coAs out of the peroxisomes. The obtained Δpox1 [pox2+, crot+] strain grew at a normal rate. The effect of these genetic modifications on fatty acid accumulation and profile was investigated when the strains were grown on oleic acids-containing medium. It was determined that the engineered strains Δpox1 [pox2+] and Δpox1 [pox2+, crot+] had increased fatty acid accumulation and an increased ratio of MCFAs. Compared to the wild-type (WT) strain, the total fatty acid production of the strains Δpox1 [pox2+] and Δpox1 [pox2+, crot+] were increased 29.5% and 15.6%, respectively. The intracellular level of MCFAs in Δpox1 [pox2+] and Δpox1 [pox2+, crot+] increased 2.26- and 1.87-fold compared to the WT strain, respectively. In addition, MCFAs in the culture medium increased 3.29-fold and 3.34-fold compared to the WT strain. These results suggested that fatty acids with an increased MCFAs ratio accumulate in the engineered strains with a modified β-oxidation pathway. Our approach exhibits great potential for transforming low value fatty acid-rich feedstock into high value fatty acid-derived products. |
| doi_str_mv | 10.1371/journal.pone.0084853 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_1490992841</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_da6e70f64df642bb9e8ac66ed41a8a0c</doaj_id><sourcerecordid>1492687221</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-e3e65888becb860c6d0f2489edcb4146aad7f19bab8386409f481cc5d223a0c13</originalsourceid><addsrcrecordid>eNptUsFu1DAQjRCIlsIfILDEhcsutuM4zgWpqgpUqsQBOFsTe7LrVdYOtlPYI7_Eh_BNZHfTqkUcLFvj997MG72ieMnokpU1e7cJY_TQL4fgcUmpEqoqHxWnrCn5QnJaPr73PimepbShtCqVlE-LEy6ErISgp8WvS79yHjE6vyJ5jeQLGLOGGLY7g4kYjHjjkgMkf34vwk9nIbvgyQB5_QN2JAfivIkICckWrRu3ZGI7TzrIeUfAOEuGGOxoDjRIZAgZfXbQk9aFbsT-efGkgz7hi_k-K759uPx68Wlx_fnj1cX59cJUXOYFligrpVSLplWSGmlpx4Vq0JpWMCEBbN2xpoVWTSYFbTqhmDGV5bwEalh5Vrw-6g59SHreXtJMNLRpuBJ7xNURYQNs9BDdFuJOB3D6UAhxpSFmZ3rUFiTWtJPCToe3bYMKjJRoBQM1tZu03s_dxnZajJk8R-gfiD788W6tV-FGl6qpBeWTwNtZIIbvI6asty4Z7HvwGMbD3FyqmvP93G_-gf7fnTiiTAwpRezuhmFU7xN1y9L7ROk5URPt1X0jd6TbCJV_AdBAznI</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1490992841</pqid></control><display><type>article</type><title>Engineering the Saccharomyces cerevisiae β-oxidation pathway to increase medium chain fatty acid production as potential biofuel</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>MEDLINE</source><source>Full-Text Journals in Chemistry (Open access)</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central</source><source>EZB Electronic Journals Library</source><creator>Chen, Liwei ; Zhang, Jianhua ; Chen, Wei Ning</creator><creatorcontrib>Chen, Liwei ; Zhang, Jianhua ; Chen, Wei Ning</creatorcontrib><description>Fatty acid-derived biofuels and biochemicals can be produced in microbes using β-oxidation pathway engineering. In this study, the β-oxidation pathway of Saccharomyces cerevisiae was engineered to accumulate a higher ratio of medium chain fatty acids (MCFAs) when cells were grown on fatty acid-rich feedstock. For this purpose, the haploid deletion strain Δpox1 was obtained, in which the sole acyl-CoA oxidase encoded by POX1 was deleted. Next, the POX2 gene from Yarrowia lipolytica, which encodes an acyl-CoA oxidase with a preference for long chain acyl-CoAs, was expressed in the Δpox1 strain. The resulting Δpox1 [pox2+] strain exhibited a growth defect because the β-oxidation pathway was blocked in peroxisomes. To unblock the β-oxidation pathway, the gene CROT, which encodes carnitine O-octanoyltransferase, was expressed in the Δpox1 [pox2+] strain to transport the accumulated medium chain acyl-coAs out of the peroxisomes. The obtained Δpox1 [pox2+, crot+] strain grew at a normal rate. The effect of these genetic modifications on fatty acid accumulation and profile was investigated when the strains were grown on oleic acids-containing medium. It was determined that the engineered strains Δpox1 [pox2+] and Δpox1 [pox2+, crot+] had increased fatty acid accumulation and an increased ratio of MCFAs. Compared to the wild-type (WT) strain, the total fatty acid production of the strains Δpox1 [pox2+] and Δpox1 [pox2+, crot+] were increased 29.5% and 15.6%, respectively. The intracellular level of MCFAs in Δpox1 [pox2+] and Δpox1 [pox2+, crot+] increased 2.26- and 1.87-fold compared to the WT strain, respectively. In addition, MCFAs in the culture medium increased 3.29-fold and 3.34-fold compared to the WT strain. These results suggested that fatty acids with an increased MCFAs ratio accumulate in the engineered strains with a modified β-oxidation pathway. Our approach exhibits great potential for transforming low value fatty acid-rich feedstock into high value fatty acid-derived products.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0084853</identifier><identifier>PMID: 24465440</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accumulation ; Acid production ; Acyl Coenzyme A - metabolism ; Acyl-CoA oxidase ; Acyl-CoA Oxidase - deficiency ; Acyl-CoA Oxidase - genetics ; Biodiesel fuels ; Biofuels ; Biology ; Carnitine ; Carnitine Acyltransferases - genetics ; Carnitine Acyltransferases - metabolism ; Carnitine O-octanoyltransferase ; Cell culture ; Chains ; Clonal deletion ; Cytoplasm - enzymology ; Defects ; Engineering ; Fatty acids ; Fatty Acids - biosynthesis ; Isoenzymes - genetics ; Isoenzymes - metabolism ; Lipids ; Metabolic Engineering ; Mus musculus ; Oxidase ; Oxidation ; Oxidation-Reduction ; Peroxisomes ; Peroxisomes - enzymology ; Raw materials ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins - genetics ; Transgenes ; Yarrowia - chemistry ; Yarrowia - enzymology ; Yarrowia lipolytica ; Yeast</subject><ispartof>PloS one, 2014, Vol.9 (1), p.e84853-e84853</ispartof><rights>2014 Chen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Chen et al 2014 Chen et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-e3e65888becb860c6d0f2489edcb4146aad7f19bab8386409f481cc5d223a0c13</citedby><cites>FETCH-LOGICAL-c526t-e3e65888becb860c6d0f2489edcb4146aad7f19bab8386409f481cc5d223a0c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897402/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897402/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,4010,23845,27900,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24465440$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Liwei</creatorcontrib><creatorcontrib>Zhang, Jianhua</creatorcontrib><creatorcontrib>Chen, Wei Ning</creatorcontrib><title>Engineering the Saccharomyces cerevisiae β-oxidation pathway to increase medium chain fatty acid production as potential biofuel</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Fatty acid-derived biofuels and biochemicals can be produced in microbes using β-oxidation pathway engineering. In this study, the β-oxidation pathway of Saccharomyces cerevisiae was engineered to accumulate a higher ratio of medium chain fatty acids (MCFAs) when cells were grown on fatty acid-rich feedstock. For this purpose, the haploid deletion strain Δpox1 was obtained, in which the sole acyl-CoA oxidase encoded by POX1 was deleted. Next, the POX2 gene from Yarrowia lipolytica, which encodes an acyl-CoA oxidase with a preference for long chain acyl-CoAs, was expressed in the Δpox1 strain. The resulting Δpox1 [pox2+] strain exhibited a growth defect because the β-oxidation pathway was blocked in peroxisomes. To unblock the β-oxidation pathway, the gene CROT, which encodes carnitine O-octanoyltransferase, was expressed in the Δpox1 [pox2+] strain to transport the accumulated medium chain acyl-coAs out of the peroxisomes. The obtained Δpox1 [pox2+, crot+] strain grew at a normal rate. The effect of these genetic modifications on fatty acid accumulation and profile was investigated when the strains were grown on oleic acids-containing medium. It was determined that the engineered strains Δpox1 [pox2+] and Δpox1 [pox2+, crot+] had increased fatty acid accumulation and an increased ratio of MCFAs. Compared to the wild-type (WT) strain, the total fatty acid production of the strains Δpox1 [pox2+] and Δpox1 [pox2+, crot+] were increased 29.5% and 15.6%, respectively. The intracellular level of MCFAs in Δpox1 [pox2+] and Δpox1 [pox2+, crot+] increased 2.26- and 1.87-fold compared to the WT strain, respectively. In addition, MCFAs in the culture medium increased 3.29-fold and 3.34-fold compared to the WT strain. These results suggested that fatty acids with an increased MCFAs ratio accumulate in the engineered strains with a modified β-oxidation pathway. Our approach exhibits great potential for transforming low value fatty acid-rich feedstock into high value fatty acid-derived products.</description><subject>Accumulation</subject><subject>Acid production</subject><subject>Acyl Coenzyme A - metabolism</subject><subject>Acyl-CoA oxidase</subject><subject>Acyl-CoA Oxidase - deficiency</subject><subject>Acyl-CoA Oxidase - genetics</subject><subject>Biodiesel fuels</subject><subject>Biofuels</subject><subject>Biology</subject><subject>Carnitine</subject><subject>Carnitine Acyltransferases - genetics</subject><subject>Carnitine Acyltransferases - metabolism</subject><subject>Carnitine O-octanoyltransferase</subject><subject>Cell culture</subject><subject>Chains</subject><subject>Clonal deletion</subject><subject>Cytoplasm - enzymology</subject><subject>Defects</subject><subject>Engineering</subject><subject>Fatty acids</subject><subject>Fatty Acids - biosynthesis</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - metabolism</subject><subject>Lipids</subject><subject>Metabolic Engineering</subject><subject>Mus musculus</subject><subject>Oxidase</subject><subject>Oxidation</subject><subject>Oxidation-Reduction</subject><subject>Peroxisomes</subject><subject>Peroxisomes - enzymology</subject><subject>Raw materials</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Transgenes</subject><subject>Yarrowia - chemistry</subject><subject>Yarrowia - enzymology</subject><subject>Yarrowia lipolytica</subject><subject>Yeast</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptUsFu1DAQjRCIlsIfILDEhcsutuM4zgWpqgpUqsQBOFsTe7LrVdYOtlPYI7_Eh_BNZHfTqkUcLFvj997MG72ieMnokpU1e7cJY_TQL4fgcUmpEqoqHxWnrCn5QnJaPr73PimepbShtCqVlE-LEy6ErISgp8WvS79yHjE6vyJ5jeQLGLOGGLY7g4kYjHjjkgMkf34vwk9nIbvgyQB5_QN2JAfivIkICckWrRu3ZGI7TzrIeUfAOEuGGOxoDjRIZAgZfXbQk9aFbsT-efGkgz7hi_k-K759uPx68Wlx_fnj1cX59cJUXOYFligrpVSLplWSGmlpx4Vq0JpWMCEBbN2xpoVWTSYFbTqhmDGV5bwEalh5Vrw-6g59SHreXtJMNLRpuBJ7xNURYQNs9BDdFuJOB3D6UAhxpSFmZ3rUFiTWtJPCToe3bYMKjJRoBQM1tZu03s_dxnZajJk8R-gfiD788W6tV-FGl6qpBeWTwNtZIIbvI6asty4Z7HvwGMbD3FyqmvP93G_-gf7fnTiiTAwpRezuhmFU7xN1y9L7ROk5URPt1X0jd6TbCJV_AdBAznI</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Chen, Liwei</creator><creator>Zhang, Jianhua</creator><creator>Chen, Wei Ning</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>2014</creationdate><title>Engineering the Saccharomyces cerevisiae β-oxidation pathway to increase medium chain fatty acid production as potential biofuel</title><author>Chen, Liwei ; Zhang, Jianhua ; Chen, Wei Ning</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-e3e65888becb860c6d0f2489edcb4146aad7f19bab8386409f481cc5d223a0c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Accumulation</topic><topic>Acid production</topic><topic>Acyl Coenzyme A - metabolism</topic><topic>Acyl-CoA oxidase</topic><topic>Acyl-CoA Oxidase - deficiency</topic><topic>Acyl-CoA Oxidase - genetics</topic><topic>Biodiesel fuels</topic><topic>Biofuels</topic><topic>Biology</topic><topic>Carnitine</topic><topic>Carnitine Acyltransferases - genetics</topic><topic>Carnitine Acyltransferases - metabolism</topic><topic>Carnitine O-octanoyltransferase</topic><topic>Cell culture</topic><topic>Chains</topic><topic>Clonal deletion</topic><topic>Cytoplasm - enzymology</topic><topic>Defects</topic><topic>Engineering</topic><topic>Fatty acids</topic><topic>Fatty Acids - biosynthesis</topic><topic>Isoenzymes - genetics</topic><topic>Isoenzymes - metabolism</topic><topic>Lipids</topic><topic>Metabolic Engineering</topic><topic>Mus musculus</topic><topic>Oxidase</topic><topic>Oxidation</topic><topic>Oxidation-Reduction</topic><topic>Peroxisomes</topic><topic>Peroxisomes - enzymology</topic><topic>Raw materials</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Transgenes</topic><topic>Yarrowia - chemistry</topic><topic>Yarrowia - enzymology</topic><topic>Yarrowia lipolytica</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Liwei</creatorcontrib><creatorcontrib>Zhang, Jianhua</creatorcontrib><creatorcontrib>Chen, Wei Ning</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Database (1962 - current)</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>https://resources.nclive.org/materials</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Liwei</au><au>Zhang, Jianhua</au><au>Chen, Wei Ning</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Engineering the Saccharomyces cerevisiae β-oxidation pathway to increase medium chain fatty acid production as potential biofuel</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014</date><risdate>2014</risdate><volume>9</volume><issue>1</issue><spage>e84853</spage><epage>e84853</epage><pages>e84853-e84853</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Fatty acid-derived biofuels and biochemicals can be produced in microbes using β-oxidation pathway engineering. In this study, the β-oxidation pathway of Saccharomyces cerevisiae was engineered to accumulate a higher ratio of medium chain fatty acids (MCFAs) when cells were grown on fatty acid-rich feedstock. For this purpose, the haploid deletion strain Δpox1 was obtained, in which the sole acyl-CoA oxidase encoded by POX1 was deleted. Next, the POX2 gene from Yarrowia lipolytica, which encodes an acyl-CoA oxidase with a preference for long chain acyl-CoAs, was expressed in the Δpox1 strain. The resulting Δpox1 [pox2+] strain exhibited a growth defect because the β-oxidation pathway was blocked in peroxisomes. To unblock the β-oxidation pathway, the gene CROT, which encodes carnitine O-octanoyltransferase, was expressed in the Δpox1 [pox2+] strain to transport the accumulated medium chain acyl-coAs out of the peroxisomes. The obtained Δpox1 [pox2+, crot+] strain grew at a normal rate. The effect of these genetic modifications on fatty acid accumulation and profile was investigated when the strains were grown on oleic acids-containing medium. It was determined that the engineered strains Δpox1 [pox2+] and Δpox1 [pox2+, crot+] had increased fatty acid accumulation and an increased ratio of MCFAs. Compared to the wild-type (WT) strain, the total fatty acid production of the strains Δpox1 [pox2+] and Δpox1 [pox2+, crot+] were increased 29.5% and 15.6%, respectively. The intracellular level of MCFAs in Δpox1 [pox2+] and Δpox1 [pox2+, crot+] increased 2.26- and 1.87-fold compared to the WT strain, respectively. In addition, MCFAs in the culture medium increased 3.29-fold and 3.34-fold compared to the WT strain. These results suggested that fatty acids with an increased MCFAs ratio accumulate in the engineered strains with a modified β-oxidation pathway. Our approach exhibits great potential for transforming low value fatty acid-rich feedstock into high value fatty acid-derived products.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24465440</pmid><doi>10.1371/journal.pone.0084853</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014, Vol.9 (1), p.e84853-e84853 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1490992841 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; Full-Text Journals in Chemistry (Open access); DOAJ Directory of Open Access Journals; PubMed Central; EZB Electronic Journals Library |
| subjects | Accumulation Acid production Acyl Coenzyme A - metabolism Acyl-CoA oxidase Acyl-CoA Oxidase - deficiency Acyl-CoA Oxidase - genetics Biodiesel fuels Biofuels Biology Carnitine Carnitine Acyltransferases - genetics Carnitine Acyltransferases - metabolism Carnitine O-octanoyltransferase Cell culture Chains Clonal deletion Cytoplasm - enzymology Defects Engineering Fatty acids Fatty Acids - biosynthesis Isoenzymes - genetics Isoenzymes - metabolism Lipids Metabolic Engineering Mus musculus Oxidase Oxidation Oxidation-Reduction Peroxisomes Peroxisomes - enzymology Raw materials Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins - genetics Transgenes Yarrowia - chemistry Yarrowia - enzymology Yarrowia lipolytica Yeast |
| title | Engineering the Saccharomyces cerevisiae β-oxidation pathway to increase medium chain fatty acid production as potential biofuel |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T18%3A05%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Engineering%20the%20Saccharomyces%20cerevisiae%20%CE%B2-oxidation%20pathway%20to%20increase%20medium%20chain%20fatty%20acid%20production%20as%20potential%20biofuel&rft.jtitle=PloS%20one&rft.au=Chen,%20Liwei&rft.date=2014&rft.volume=9&rft.issue=1&rft.spage=e84853&rft.epage=e84853&rft.pages=e84853-e84853&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0084853&rft_dat=%3Cproquest_plos_%3E1492687221%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1490992841&rft_id=info:pmid/24465440&rft_doaj_id=oai_doaj_org_article_da6e70f64df642bb9e8ac66ed41a8a0c&rfr_iscdi=true |