Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight
Fetal growth involves highly complex molecular pathways. IGF2 is a key paternally expressed growth hormone that is critical for in utero growth in mice. Its role in human fetal growth has remained ambiguous, as it has only been studied in term tissues. Conversely the maternally expressed growth supp...
Gespeichert in:
| Veröffentlicht in: | PloS one 2014-01, Vol.9 (1), p.e85454-e85454 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e85454 |
|---|---|
| container_issue | 1 |
| container_start_page | e85454 |
| container_title | PloS one |
| container_volume | 9 |
| creator | Demetriou, Charalambos Abu-Amero, Sayeda Thomas, Anna C Ishida, Miho Aggarwal, Reena Al-Olabi, Lara Leon, Lydia J Stafford, Jaime L Syngelaki, Argyro Peebles, Donald Nicolaides, Kypros H Regan, Lesley Stanier, Philip Moore, Gudrun E |
| description | Fetal growth involves highly complex molecular pathways. IGF2 is a key paternally expressed growth hormone that is critical for in utero growth in mice. Its role in human fetal growth has remained ambiguous, as it has only been studied in term tissues. Conversely the maternally expressed growth suppressor, PHLDA2, has a significant negative correlation between its term placental expression and birth weight.
The aim of this study is to address the role in early gestation of expression of IGF1, IGF2, their receptors IGF1R and IGF2R, and PHLDA2 on term birth weight.
Real-time quantitative PCR was used to investigate mRNA expression of IGF1, IGF2, IGF1R, IGF2R and PHLDA2 in chorionic villus samples (CVS) (n = 260) collected at 11-13 weeks' gestation. Expression was correlated with term birth weight using statistical package R including correction for several confounding factors.
Transcript levels of IGF2 and IGF2R revealed a significant positive correlation with birth weight (0.009 and 0.04, respectively). No effect was observed for IGF1, IGF1R or PHLDA2 and birth weight. Critically, small for gestational age (SGA) neonates had significantly lower IGF2 levels than appropriate for gestational age neonates (p = 3.6 × 10(-7)).
Our findings show that IGF2 mRNA levels at 12 weeks gestation could provide a useful predictor of future fetal growth to term, potentially predicting SGA babies. SGA babies are known to be at a higher risk for type 2 diabetes. This research reveals an imprinted, parentally driven rheostat for in utero growth. |
| doi_str_mv | 10.1371/journal.pone.0085454 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1477787467</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478862819</galeid><doaj_id>oai_doaj_org_article_08749f3843fc44aa87d216e6ca42c13d</doaj_id><sourcerecordid>A478862819</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-54d5f6a14f75eff26719a7b95969c73d2b8c6cc6067813ccda0be38dc817b08f3</originalsourceid><addsrcrecordid>eNqNk12L1DAUhoso7rr6D0QLgijYsWnSJL0RlsWPgYUVv25DmiZtxkwyJunOrr_e1OkuM7IXUkhD8rzvyTnJybKnoFwASMDblRu95WaxcVYuypLWqEb3smPQwKrAVQnv782PskchrMqyhhTjh9lRhRJMCTjOfn_mUU4-5jqXVxsvQ5Ddm1yvN17bKLtc2zAabQujf8q8924bh1xxEZ0vqrSZi8F57awW-aU2RufCeS9NMg150L3VSgtuY3Lf6qRstU_jVup-iI-zB4qbIJ_M_5Ps-4f3384-FecXH5dnp-eFwE0Vixp1tcIcIEVqqVSFCWg4aZu6wY0gsKtaKrAQuMSEAihEx8tWQtoJCkhbUgVPsuc7341xgc1lCwwgQgglCJNELHdE5_iKpczX3F8zxzX7u-B8z7iPWhjJyqRoFKQIKoEQ55R0FcASC44qAWCXvN7N0cZ2LTshbfTcHJge7lg9sN5dMkgbCJomGbyaDbz7NcoQ2VoHIY3hVrpxOndT4QZDAhP64h_07uxmqucpAW2VS3HFZMpOEaEUVxRMYRd3UOnr5FqL9MaUTusHgtcHgsREeRV7PobAll-__D978eOQfbnHDpKbOARnxpheWTgE0Q4U3oXgpbotMijZ1CI31WBTi7C5RZLs2f4F3YpuegL-AdwDDlA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1477787467</pqid></control><display><type>article</type><title>Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Demetriou, Charalambos ; Abu-Amero, Sayeda ; Thomas, Anna C ; Ishida, Miho ; Aggarwal, Reena ; Al-Olabi, Lara ; Leon, Lydia J ; Stafford, Jaime L ; Syngelaki, Argyro ; Peebles, Donald ; Nicolaides, Kypros H ; Regan, Lesley ; Stanier, Philip ; Moore, Gudrun E</creator><contributor>Oudejans, Cees</contributor><creatorcontrib>Demetriou, Charalambos ; Abu-Amero, Sayeda ; Thomas, Anna C ; Ishida, Miho ; Aggarwal, Reena ; Al-Olabi, Lara ; Leon, Lydia J ; Stafford, Jaime L ; Syngelaki, Argyro ; Peebles, Donald ; Nicolaides, Kypros H ; Regan, Lesley ; Stanier, Philip ; Moore, Gudrun E ; Oudejans, Cees</creatorcontrib><description>Fetal growth involves highly complex molecular pathways. IGF2 is a key paternally expressed growth hormone that is critical for in utero growth in mice. Its role in human fetal growth has remained ambiguous, as it has only been studied in term tissues. Conversely the maternally expressed growth suppressor, PHLDA2, has a significant negative correlation between its term placental expression and birth weight.
The aim of this study is to address the role in early gestation of expression of IGF1, IGF2, their receptors IGF1R and IGF2R, and PHLDA2 on term birth weight.
Real-time quantitative PCR was used to investigate mRNA expression of IGF1, IGF2, IGF1R, IGF2R and PHLDA2 in chorionic villus samples (CVS) (n = 260) collected at 11-13 weeks' gestation. Expression was correlated with term birth weight using statistical package R including correction for several confounding factors.
Transcript levels of IGF2 and IGF2R revealed a significant positive correlation with birth weight (0.009 and 0.04, respectively). No effect was observed for IGF1, IGF1R or PHLDA2 and birth weight. Critically, small for gestational age (SGA) neonates had significantly lower IGF2 levels than appropriate for gestational age neonates (p = 3.6 × 10(-7)).
Our findings show that IGF2 mRNA levels at 12 weeks gestation could provide a useful predictor of future fetal growth to term, potentially predicting SGA babies. SGA babies are known to be at a higher risk for type 2 diabetes. This research reveals an imprinted, parentally driven rheostat for in utero growth.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0085454</identifier><identifier>PMID: 24454871</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Age ; Animal tissues ; Babies ; Base Sequence ; Biology ; Birth Weight ; Childbirth & labor ; Childrens health ; Chorionic Villi - metabolism ; College campuses ; Correlation ; Diabetes ; Diabetes mellitus ; DNA Primers ; Fetal development ; Fetuses ; Gene expression ; Genomes ; Genomic Imprinting ; Gestation ; Gestational age ; Growth hormone ; Growth hormones ; Gynecology ; Health risks ; Humans ; Insulin ; Insulin resistance ; Insulin-like growth factor I ; Insulin-like growth factor II ; Insulin-Like Growth Factor II - genetics ; Insulin-like growth factor II receptors ; Insulin-like growth factors ; Male ; Mathematics ; Medicine ; Neonates ; Newborn babies ; Obstetrics ; Placenta ; Pregnancy ; Premature birth ; Proteins ; Real-Time Polymerase Chain Reaction ; Receptors ; Reverse Transcriptase Polymerase Chain Reaction ; RNA ; Small for gestational age ; Somatotropin ; Statistical analysis ; Statistical methods ; Studies ; Systematic review ; Transcription ; Type 2 diabetes ; Villus ; Womens health</subject><ispartof>PloS one, 2014-01, Vol.9 (1), p.e85454-e85454</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Demetriou et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Demetriou et al 2014 Demetriou et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-54d5f6a14f75eff26719a7b95969c73d2b8c6cc6067813ccda0be38dc817b08f3</citedby><cites>FETCH-LOGICAL-c692t-54d5f6a14f75eff26719a7b95969c73d2b8c6cc6067813ccda0be38dc817b08f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893199/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893199/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24454871$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Oudejans, Cees</contributor><creatorcontrib>Demetriou, Charalambos</creatorcontrib><creatorcontrib>Abu-Amero, Sayeda</creatorcontrib><creatorcontrib>Thomas, Anna C</creatorcontrib><creatorcontrib>Ishida, Miho</creatorcontrib><creatorcontrib>Aggarwal, Reena</creatorcontrib><creatorcontrib>Al-Olabi, Lara</creatorcontrib><creatorcontrib>Leon, Lydia J</creatorcontrib><creatorcontrib>Stafford, Jaime L</creatorcontrib><creatorcontrib>Syngelaki, Argyro</creatorcontrib><creatorcontrib>Peebles, Donald</creatorcontrib><creatorcontrib>Nicolaides, Kypros H</creatorcontrib><creatorcontrib>Regan, Lesley</creatorcontrib><creatorcontrib>Stanier, Philip</creatorcontrib><creatorcontrib>Moore, Gudrun E</creatorcontrib><title>Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Fetal growth involves highly complex molecular pathways. IGF2 is a key paternally expressed growth hormone that is critical for in utero growth in mice. Its role in human fetal growth has remained ambiguous, as it has only been studied in term tissues. Conversely the maternally expressed growth suppressor, PHLDA2, has a significant negative correlation between its term placental expression and birth weight.
The aim of this study is to address the role in early gestation of expression of IGF1, IGF2, their receptors IGF1R and IGF2R, and PHLDA2 on term birth weight.
Real-time quantitative PCR was used to investigate mRNA expression of IGF1, IGF2, IGF1R, IGF2R and PHLDA2 in chorionic villus samples (CVS) (n = 260) collected at 11-13 weeks' gestation. Expression was correlated with term birth weight using statistical package R including correction for several confounding factors.
Transcript levels of IGF2 and IGF2R revealed a significant positive correlation with birth weight (0.009 and 0.04, respectively). No effect was observed for IGF1, IGF1R or PHLDA2 and birth weight. Critically, small for gestational age (SGA) neonates had significantly lower IGF2 levels than appropriate for gestational age neonates (p = 3.6 × 10(-7)).
Our findings show that IGF2 mRNA levels at 12 weeks gestation could provide a useful predictor of future fetal growth to term, potentially predicting SGA babies. SGA babies are known to be at a higher risk for type 2 diabetes. This research reveals an imprinted, parentally driven rheostat for in utero growth.</description><subject>Age</subject><subject>Animal tissues</subject><subject>Babies</subject><subject>Base Sequence</subject><subject>Biology</subject><subject>Birth Weight</subject><subject>Childbirth & labor</subject><subject>Childrens health</subject><subject>Chorionic Villi - metabolism</subject><subject>College campuses</subject><subject>Correlation</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>DNA Primers</subject><subject>Fetal development</subject><subject>Fetuses</subject><subject>Gene expression</subject><subject>Genomes</subject><subject>Genomic Imprinting</subject><subject>Gestation</subject><subject>Gestational age</subject><subject>Growth hormone</subject><subject>Growth hormones</subject><subject>Gynecology</subject><subject>Health risks</subject><subject>Humans</subject><subject>Insulin</subject><subject>Insulin resistance</subject><subject>Insulin-like growth factor I</subject><subject>Insulin-like growth factor II</subject><subject>Insulin-Like Growth Factor II - genetics</subject><subject>Insulin-like growth factor II receptors</subject><subject>Insulin-like growth factors</subject><subject>Male</subject><subject>Mathematics</subject><subject>Medicine</subject><subject>Neonates</subject><subject>Newborn babies</subject><subject>Obstetrics</subject><subject>Placenta</subject><subject>Pregnancy</subject><subject>Premature birth</subject><subject>Proteins</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptors</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA</subject><subject>Small for gestational age</subject><subject>Somatotropin</subject><subject>Statistical analysis</subject><subject>Statistical methods</subject><subject>Studies</subject><subject>Systematic review</subject><subject>Transcription</subject><subject>Type 2 diabetes</subject><subject>Villus</subject><subject>Womens health</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLgijYsWnSJL0RlsWPgYUVv25DmiZtxkwyJunOrr_e1OkuM7IXUkhD8rzvyTnJybKnoFwASMDblRu95WaxcVYuypLWqEb3smPQwKrAVQnv782PskchrMqyhhTjh9lRhRJMCTjOfn_mUU4-5jqXVxsvQ5Ddm1yvN17bKLtc2zAabQujf8q8924bh1xxEZ0vqrSZi8F57awW-aU2RufCeS9NMg150L3VSgtuY3Lf6qRstU_jVup-iI-zB4qbIJ_M_5Ps-4f3384-FecXH5dnp-eFwE0Vixp1tcIcIEVqqVSFCWg4aZu6wY0gsKtaKrAQuMSEAihEx8tWQtoJCkhbUgVPsuc7341xgc1lCwwgQgglCJNELHdE5_iKpczX3F8zxzX7u-B8z7iPWhjJyqRoFKQIKoEQ55R0FcASC44qAWCXvN7N0cZ2LTshbfTcHJge7lg9sN5dMkgbCJomGbyaDbz7NcoQ2VoHIY3hVrpxOndT4QZDAhP64h_07uxmqucpAW2VS3HFZMpOEaEUVxRMYRd3UOnr5FqL9MaUTusHgtcHgsREeRV7PobAll-__D978eOQfbnHDpKbOARnxpheWTgE0Q4U3oXgpbotMijZ1CI31WBTi7C5RZLs2f4F3YpuegL-AdwDDlA</recordid><startdate>20140115</startdate><enddate>20140115</enddate><creator>Demetriou, Charalambos</creator><creator>Abu-Amero, Sayeda</creator><creator>Thomas, Anna C</creator><creator>Ishida, Miho</creator><creator>Aggarwal, Reena</creator><creator>Al-Olabi, Lara</creator><creator>Leon, Lydia J</creator><creator>Stafford, Jaime L</creator><creator>Syngelaki, Argyro</creator><creator>Peebles, Donald</creator><creator>Nicolaides, Kypros H</creator><creator>Regan, Lesley</creator><creator>Stanier, Philip</creator><creator>Moore, Gudrun E</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140115</creationdate><title>Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight</title><author>Demetriou, Charalambos ; Abu-Amero, Sayeda ; Thomas, Anna C ; Ishida, Miho ; Aggarwal, Reena ; Al-Olabi, Lara ; Leon, Lydia J ; Stafford, Jaime L ; Syngelaki, Argyro ; Peebles, Donald ; Nicolaides, Kypros H ; Regan, Lesley ; Stanier, Philip ; Moore, Gudrun E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-54d5f6a14f75eff26719a7b95969c73d2b8c6cc6067813ccda0be38dc817b08f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Age</topic><topic>Animal tissues</topic><topic>Babies</topic><topic>Base Sequence</topic><topic>Biology</topic><topic>Birth Weight</topic><topic>Childbirth & labor</topic><topic>Childrens health</topic><topic>Chorionic Villi - metabolism</topic><topic>College campuses</topic><topic>Correlation</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>DNA Primers</topic><topic>Fetal development</topic><topic>Fetuses</topic><topic>Gene expression</topic><topic>Genomes</topic><topic>Genomic Imprinting</topic><topic>Gestation</topic><topic>Gestational age</topic><topic>Growth hormone</topic><topic>Growth hormones</topic><topic>Gynecology</topic><topic>Health risks</topic><topic>Humans</topic><topic>Insulin</topic><topic>Insulin resistance</topic><topic>Insulin-like growth factor I</topic><topic>Insulin-like growth factor II</topic><topic>Insulin-Like Growth Factor II - genetics</topic><topic>Insulin-like growth factor II receptors</topic><topic>Insulin-like growth factors</topic><topic>Male</topic><topic>Mathematics</topic><topic>Medicine</topic><topic>Neonates</topic><topic>Newborn babies</topic><topic>Obstetrics</topic><topic>Placenta</topic><topic>Pregnancy</topic><topic>Premature birth</topic><topic>Proteins</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptors</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA</topic><topic>Small for gestational age</topic><topic>Somatotropin</topic><topic>Statistical analysis</topic><topic>Statistical methods</topic><topic>Studies</topic><topic>Systematic review</topic><topic>Transcription</topic><topic>Type 2 diabetes</topic><topic>Villus</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Demetriou, Charalambos</creatorcontrib><creatorcontrib>Abu-Amero, Sayeda</creatorcontrib><creatorcontrib>Thomas, Anna C</creatorcontrib><creatorcontrib>Ishida, Miho</creatorcontrib><creatorcontrib>Aggarwal, Reena</creatorcontrib><creatorcontrib>Al-Olabi, Lara</creatorcontrib><creatorcontrib>Leon, Lydia J</creatorcontrib><creatorcontrib>Stafford, Jaime L</creatorcontrib><creatorcontrib>Syngelaki, Argyro</creatorcontrib><creatorcontrib>Peebles, Donald</creatorcontrib><creatorcontrib>Nicolaides, Kypros H</creatorcontrib><creatorcontrib>Regan, Lesley</creatorcontrib><creatorcontrib>Stanier, Philip</creatorcontrib><creatorcontrib>Moore, Gudrun E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Opposing Viewpoints in Context (Gale)</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Demetriou, Charalambos</au><au>Abu-Amero, Sayeda</au><au>Thomas, Anna C</au><au>Ishida, Miho</au><au>Aggarwal, Reena</au><au>Al-Olabi, Lara</au><au>Leon, Lydia J</au><au>Stafford, Jaime L</au><au>Syngelaki, Argyro</au><au>Peebles, Donald</au><au>Nicolaides, Kypros H</au><au>Regan, Lesley</au><au>Stanier, Philip</au><au>Moore, Gudrun E</au><au>Oudejans, Cees</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-01-15</date><risdate>2014</risdate><volume>9</volume><issue>1</issue><spage>e85454</spage><epage>e85454</epage><pages>e85454-e85454</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Fetal growth involves highly complex molecular pathways. IGF2 is a key paternally expressed growth hormone that is critical for in utero growth in mice. Its role in human fetal growth has remained ambiguous, as it has only been studied in term tissues. Conversely the maternally expressed growth suppressor, PHLDA2, has a significant negative correlation between its term placental expression and birth weight.
The aim of this study is to address the role in early gestation of expression of IGF1, IGF2, their receptors IGF1R and IGF2R, and PHLDA2 on term birth weight.
Real-time quantitative PCR was used to investigate mRNA expression of IGF1, IGF2, IGF1R, IGF2R and PHLDA2 in chorionic villus samples (CVS) (n = 260) collected at 11-13 weeks' gestation. Expression was correlated with term birth weight using statistical package R including correction for several confounding factors.
Transcript levels of IGF2 and IGF2R revealed a significant positive correlation with birth weight (0.009 and 0.04, respectively). No effect was observed for IGF1, IGF1R or PHLDA2 and birth weight. Critically, small for gestational age (SGA) neonates had significantly lower IGF2 levels than appropriate for gestational age neonates (p = 3.6 × 10(-7)).
Our findings show that IGF2 mRNA levels at 12 weeks gestation could provide a useful predictor of future fetal growth to term, potentially predicting SGA babies. SGA babies are known to be at a higher risk for type 2 diabetes. This research reveals an imprinted, parentally driven rheostat for in utero growth.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24454871</pmid><doi>10.1371/journal.pone.0085454</doi><tpages>e85454</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-01, Vol.9 (1), p.e85454-e85454 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1477787467 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Age Animal tissues Babies Base Sequence Biology Birth Weight Childbirth & labor Childrens health Chorionic Villi - metabolism College campuses Correlation Diabetes Diabetes mellitus DNA Primers Fetal development Fetuses Gene expression Genomes Genomic Imprinting Gestation Gestational age Growth hormone Growth hormones Gynecology Health risks Humans Insulin Insulin resistance Insulin-like growth factor I Insulin-like growth factor II Insulin-Like Growth Factor II - genetics Insulin-like growth factor II receptors Insulin-like growth factors Male Mathematics Medicine Neonates Newborn babies Obstetrics Placenta Pregnancy Premature birth Proteins Real-Time Polymerase Chain Reaction Receptors Reverse Transcriptase Polymerase Chain Reaction RNA Small for gestational age Somatotropin Statistical analysis Statistical methods Studies Systematic review Transcription Type 2 diabetes Villus Womens health |
| title | Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T04%3A31%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Paternally%20expressed,%20imprinted%20insulin-like%20growth%20factor-2%20in%20chorionic%20villi%20correlates%20significantly%20with%20birth%20weight&rft.jtitle=PloS%20one&rft.au=Demetriou,%20Charalambos&rft.date=2014-01-15&rft.volume=9&rft.issue=1&rft.spage=e85454&rft.epage=e85454&rft.pages=e85454-e85454&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0085454&rft_dat=%3Cgale_plos_%3EA478862819%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1477787467&rft_id=info:pmid/24454871&rft_galeid=A478862819&rft_doaj_id=oai_doaj_org_article_08749f3843fc44aa87d216e6ca42c13d&rfr_iscdi=true |