Ecological adaption analysis of the cotton aphid (Aphis gossypii) in different phenotypes by transcriptome comparison
The cotton aphid, Aphis gossypii Glover, is a destructive insect pest worldwide; it directly or indirectly damages (virus transmission) 300 species of host plants. Knowledge of their ecologically adaptive mechanisms at the molecular level may provide an essential and urgent method to effectively con...
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| Veröffentlicht in: | PloS one 2013-12, Vol.8 (12), p.e83180-e83180 |
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| creator | Li, Zhao-Qun Zhang, Shuai Luo, Jun-Yu Wang, Chun-Yi Lv, Li-Min Dong, Shuang-Lin Cui, Jin-Jie |
| description | The cotton aphid, Aphis gossypii Glover, is a destructive insect pest worldwide; it directly or indirectly damages (virus transmission) 300 species of host plants. Knowledge of their ecologically adaptive mechanisms at the molecular level may provide an essential and urgent method to effectively control this pest. However, no transcriptome information is available for the cotton aphid and sequence data are scarce. Therefore, we obtained transcriptome data.
To facilitate such a study, two cotton aphid transcriptomes at different growth stages of cotton, seedling and summer, were sequenced. A total of 161,396 and 66,668 contigs were obtained and assembled into 83,671 and 42,438 transcripts, respectively. After combining the raw date for both transcriptomes, the sequences were reassembled into 66,695 transcripts, and 52,160 were annotated based on BLASTX analyses. Comparison of the transcriptomes revealed that summer presented less challenges for the cotton aphids than the seedling stage of cotton. In total, 58 putative heat shock protein genes and 66 candidate cytochrome p450 genes were identified with BLASTX.
Our results form a basis for exploring the molecular mechanisms of ecological adaption in the cotton aphid. Our study also provides a baseline for the exploration of abiotic stress responses. In addition, it provides large-scale sequence information for further studies on this species. |
| doi_str_mv | 10.1371/journal.pone.0083180 |
| format | Article |
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To facilitate such a study, two cotton aphid transcriptomes at different growth stages of cotton, seedling and summer, were sequenced. A total of 161,396 and 66,668 contigs were obtained and assembled into 83,671 and 42,438 transcripts, respectively. After combining the raw date for both transcriptomes, the sequences were reassembled into 66,695 transcripts, and 52,160 were annotated based on BLASTX analyses. Comparison of the transcriptomes revealed that summer presented less challenges for the cotton aphids than the seedling stage of cotton. In total, 58 putative heat shock protein genes and 66 candidate cytochrome p450 genes were identified with BLASTX.
Our results form a basis for exploring the molecular mechanisms of ecological adaption in the cotton aphid. Our study also provides a baseline for the exploration of abiotic stress responses. In addition, it provides large-scale sequence information for further studies on this species.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0083180</identifier><identifier>PMID: 24376660</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Adaptation, Physiological - genetics ; Analysis ; Animals ; Aphidoidea ; Aphids ; Aphids - classification ; Aphids - genetics ; Aphis gossypii ; Biology ; Cotton ; Crop diseases ; Cytochrome ; Cytochrome P-450 Enzyme System - classification ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P450 ; Disease transmission ; Ecological effects ; Ecological monitoring ; Ecosystem ; Gene expression ; Genes ; Genetic aspects ; Genomes ; Gossypium - growth & development ; Gossypium - parasitology ; Heat shock ; Heat shock proteins ; Heat-Shock Proteins - classification ; Heat-Shock Proteins - genetics ; Host plants ; Insect pests ; Insect Proteins - classification ; Insect Proteins - genetics ; Insects ; Isoenzymes - classification ; Isoenzymes - genetics ; Laboratories ; Metabolism ; Molecular modelling ; Molecular Sequence Annotation ; Pest control ; Pests ; Phenotype ; Phylogeny ; Plant viruses ; Seedlings ; Seedlings - growth & development ; Seedlings - parasitology ; Studies ; Summer ; Transcriptome ; Viruses</subject><ispartof>PloS one, 2013-12, Vol.8 (12), p.e83180-e83180</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Li et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Li et al 2013 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c593t-43cbf461ebff9931f50a5070d74114e1d0dee8d11f18a9f045f8bd9fc3cfae0a3</citedby><cites>FETCH-LOGICAL-c593t-43cbf461ebff9931f50a5070d74114e1d0dee8d11f18a9f045f8bd9fc3cfae0a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871566/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871566/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24376660$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mills, Ken</contributor><creatorcontrib>Li, Zhao-Qun</creatorcontrib><creatorcontrib>Zhang, Shuai</creatorcontrib><creatorcontrib>Luo, Jun-Yu</creatorcontrib><creatorcontrib>Wang, Chun-Yi</creatorcontrib><creatorcontrib>Lv, Li-Min</creatorcontrib><creatorcontrib>Dong, Shuang-Lin</creatorcontrib><creatorcontrib>Cui, Jin-Jie</creatorcontrib><title>Ecological adaption analysis of the cotton aphid (Aphis gossypii) in different phenotypes by transcriptome comparison</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The cotton aphid, Aphis gossypii Glover, is a destructive insect pest worldwide; it directly or indirectly damages (virus transmission) 300 species of host plants. Knowledge of their ecologically adaptive mechanisms at the molecular level may provide an essential and urgent method to effectively control this pest. However, no transcriptome information is available for the cotton aphid and sequence data are scarce. Therefore, we obtained transcriptome data.
To facilitate such a study, two cotton aphid transcriptomes at different growth stages of cotton, seedling and summer, were sequenced. A total of 161,396 and 66,668 contigs were obtained and assembled into 83,671 and 42,438 transcripts, respectively. After combining the raw date for both transcriptomes, the sequences were reassembled into 66,695 transcripts, and 52,160 were annotated based on BLASTX analyses. Comparison of the transcriptomes revealed that summer presented less challenges for the cotton aphids than the seedling stage of cotton. In total, 58 putative heat shock protein genes and 66 candidate cytochrome p450 genes were identified with BLASTX.
Our results form a basis for exploring the molecular mechanisms of ecological adaption in the cotton aphid. Our study also provides a baseline for the exploration of abiotic stress responses. In addition, it provides large-scale sequence information for further studies on this species.</description><subject>Acids</subject><subject>Adaptation, Physiological - genetics</subject><subject>Analysis</subject><subject>Animals</subject><subject>Aphidoidea</subject><subject>Aphids</subject><subject>Aphids - classification</subject><subject>Aphids - genetics</subject><subject>Aphis gossypii</subject><subject>Biology</subject><subject>Cotton</subject><subject>Crop diseases</subject><subject>Cytochrome</subject><subject>Cytochrome P-450 Enzyme System - classification</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochrome P450</subject><subject>Disease transmission</subject><subject>Ecological effects</subject><subject>Ecological monitoring</subject><subject>Ecosystem</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Gossypium - growth & development</subject><subject>Gossypium - parasitology</subject><subject>Heat shock</subject><subject>Heat shock proteins</subject><subject>Heat-Shock Proteins - classification</subject><subject>Heat-Shock Proteins - genetics</subject><subject>Host plants</subject><subject>Insect pests</subject><subject>Insect Proteins - classification</subject><subject>Insect Proteins - genetics</subject><subject>Insects</subject><subject>Isoenzymes - classification</subject><subject>Isoenzymes - genetics</subject><subject>Laboratories</subject><subject>Metabolism</subject><subject>Molecular modelling</subject><subject>Molecular Sequence Annotation</subject><subject>Pest control</subject><subject>Pests</subject><subject>Phenotype</subject><subject>Phylogeny</subject><subject>Plant viruses</subject><subject>Seedlings</subject><subject>Seedlings - growth & development</subject><subject>Seedlings - parasitology</subject><subject>Studies</subject><subject>Summer</subject><subject>Transcriptome</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptkl1rFDEUhgdRbF39B6IBb-rFrskkk2RuhKVULRS80euQycdulplkTDKF_fdm3GnpSslFwsl7nvPBW1XvEdwgzNCXQ5iil_1mDN5sIOQYcfiiukQtrte0hvjlk_dF9SalA4QN5pS-ri5qghmlFF5W040Kfdg5JXsgtRyzCx7Iwj0ml0CwIO8NUCHnOTzunQZX23IlsAspHUfnPgPngXbWmmh8BuPe-JCPo0mgO4IcpU8qujGHYcYMo4wuBf-2emVln8y75V5Vv7_d_Lr-sb77-f32enu3Vk2L85pg1VlCkemsbVuMbANlAxnUjCBEDNJQG8M1QhZx2VpIGss73VqFlZUGSryqPp64Yx-SWDaWBCIMNpTUhbmqbk8KHeRBjNENMh5FkE78C4S4EzJmp3ojFGqY7HDhEkMowbwrFZRihGvVtd3M-rpUm7rBaFX2EWV_Bj3_8W4vduFeYM5QQ2kBXC2AGP5MJmUxuKRM30tvwjT33UKGakKaIv30n_T56RbVTpYBnLeh1FUzVGwJ44jwFrOi2jyjKkebwaliL-tK_CyBnBJULC6Ixj7OiKCYzfnQjJjNKRZzlrQPT_fzmPTgRvwXNAvkfQ</recordid><startdate>20131223</startdate><enddate>20131223</enddate><creator>Li, Zhao-Qun</creator><creator>Zhang, Shuai</creator><creator>Luo, Jun-Yu</creator><creator>Wang, Chun-Yi</creator><creator>Lv, Li-Min</creator><creator>Dong, Shuang-Lin</creator><creator>Cui, Jin-Jie</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131223</creationdate><title>Ecological adaption analysis of the cotton aphid (Aphis gossypii) in different phenotypes by transcriptome comparison</title><author>Li, Zhao-Qun ; Zhang, Shuai ; Luo, Jun-Yu ; Wang, Chun-Yi ; Lv, Li-Min ; Dong, Shuang-Lin ; Cui, Jin-Jie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c593t-43cbf461ebff9931f50a5070d74114e1d0dee8d11f18a9f045f8bd9fc3cfae0a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acids</topic><topic>Adaptation, Physiological - genetics</topic><topic>Analysis</topic><topic>Animals</topic><topic>Aphidoidea</topic><topic>Aphids</topic><topic>Aphids - classification</topic><topic>Aphids - genetics</topic><topic>Aphis gossypii</topic><topic>Biology</topic><topic>Cotton</topic><topic>Crop diseases</topic><topic>Cytochrome</topic><topic>Cytochrome P-450 Enzyme System - classification</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>Cytochrome P450</topic><topic>Disease transmission</topic><topic>Ecological effects</topic><topic>Ecological monitoring</topic><topic>Ecosystem</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Gossypium - growth & development</topic><topic>Gossypium - parasitology</topic><topic>Heat shock</topic><topic>Heat shock proteins</topic><topic>Heat-Shock Proteins - classification</topic><topic>Heat-Shock Proteins - genetics</topic><topic>Host plants</topic><topic>Insect pests</topic><topic>Insect Proteins - classification</topic><topic>Insect Proteins - genetics</topic><topic>Insects</topic><topic>Isoenzymes - classification</topic><topic>Isoenzymes - genetics</topic><topic>Laboratories</topic><topic>Metabolism</topic><topic>Molecular modelling</topic><topic>Molecular Sequence Annotation</topic><topic>Pest control</topic><topic>Pests</topic><topic>Phenotype</topic><topic>Phylogeny</topic><topic>Plant viruses</topic><topic>Seedlings</topic><topic>Seedlings - growth & development</topic><topic>Seedlings - parasitology</topic><topic>Studies</topic><topic>Summer</topic><topic>Transcriptome</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Zhao-Qun</creatorcontrib><creatorcontrib>Zhang, Shuai</creatorcontrib><creatorcontrib>Luo, Jun-Yu</creatorcontrib><creatorcontrib>Wang, Chun-Yi</creatorcontrib><creatorcontrib>Lv, Li-Min</creatorcontrib><creatorcontrib>Dong, Shuang-Lin</creatorcontrib><creatorcontrib>Cui, Jin-Jie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Zhao-Qun</au><au>Zhang, Shuai</au><au>Luo, Jun-Yu</au><au>Wang, Chun-Yi</au><au>Lv, Li-Min</au><au>Dong, Shuang-Lin</au><au>Cui, Jin-Jie</au><au>Mills, Ken</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ecological adaption analysis of the cotton aphid (Aphis gossypii) in different phenotypes by transcriptome comparison</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-12-23</date><risdate>2013</risdate><volume>8</volume><issue>12</issue><spage>e83180</spage><epage>e83180</epage><pages>e83180-e83180</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The cotton aphid, Aphis gossypii Glover, is a destructive insect pest worldwide; it directly or indirectly damages (virus transmission) 300 species of host plants. Knowledge of their ecologically adaptive mechanisms at the molecular level may provide an essential and urgent method to effectively control this pest. However, no transcriptome information is available for the cotton aphid and sequence data are scarce. Therefore, we obtained transcriptome data.
To facilitate such a study, two cotton aphid transcriptomes at different growth stages of cotton, seedling and summer, were sequenced. A total of 161,396 and 66,668 contigs were obtained and assembled into 83,671 and 42,438 transcripts, respectively. After combining the raw date for both transcriptomes, the sequences were reassembled into 66,695 transcripts, and 52,160 were annotated based on BLASTX analyses. Comparison of the transcriptomes revealed that summer presented less challenges for the cotton aphids than the seedling stage of cotton. In total, 58 putative heat shock protein genes and 66 candidate cytochrome p450 genes were identified with BLASTX.
Our results form a basis for exploring the molecular mechanisms of ecological adaption in the cotton aphid. Our study also provides a baseline for the exploration of abiotic stress responses. In addition, it provides large-scale sequence information for further studies on this species.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24376660</pmid><doi>10.1371/journal.pone.0083180</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Adaptation, Physiological - genetics Analysis Animals Aphidoidea Aphids Aphids - classification Aphids - genetics Aphis gossypii Biology Cotton Crop diseases Cytochrome Cytochrome P-450 Enzyme System - classification Cytochrome P-450 Enzyme System - genetics Cytochrome P450 Disease transmission Ecological effects Ecological monitoring Ecosystem Gene expression Genes Genetic aspects Genomes Gossypium - growth & development Gossypium - parasitology Heat shock Heat shock proteins Heat-Shock Proteins - classification Heat-Shock Proteins - genetics Host plants Insect pests Insect Proteins - classification Insect Proteins - genetics Insects Isoenzymes - classification Isoenzymes - genetics Laboratories Metabolism Molecular modelling Molecular Sequence Annotation Pest control Pests Phenotype Phylogeny Plant viruses Seedlings Seedlings - growth & development Seedlings - parasitology Studies Summer Transcriptome Viruses |
| title | Ecological adaption analysis of the cotton aphid (Aphis gossypii) in different phenotypes by transcriptome comparison |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T01%3A05%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ecological%20adaption%20analysis%20of%20the%20cotton%20aphid%20(Aphis%20gossypii)%20in%20different%20phenotypes%20by%20transcriptome%20comparison&rft.jtitle=PloS%20one&rft.au=Li,%20Zhao-Qun&rft.date=2013-12-23&rft.volume=8&rft.issue=12&rft.spage=e83180&rft.epage=e83180&rft.pages=e83180-e83180&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0083180&rft_dat=%3Cgale_plos_%3EA478148937%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1470564299&rft_id=info:pmid/24376660&rft_galeid=A478148937&rft_doaj_id=oai_doaj_org_article_c157ab3e0a4e46438b0a3cc748dcb9b9&rfr_iscdi=true |