Deficiency of MIWI2 (Piwil4) induces mouse erythroleukemia cell differentiation, but has no effect on hematopoiesis in vivo
Piwi proteins and their small non-coding RNA partners are involved in the maintenance of stem cell character and genome integrity in the male germ cells of mammals. MIWI2, one of the mouse Piwi-like proteins, is expressed in the prepachytene phase of spermatogenesis during the period of de novo meth...
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| description | Piwi proteins and their small non-coding RNA partners are involved in the maintenance of stem cell character and genome integrity in the male germ cells of mammals. MIWI2, one of the mouse Piwi-like proteins, is expressed in the prepachytene phase of spermatogenesis during the period of de novo methylation. Absence of this protein leads to meiotic defects and a progressive loss of germ cells. There is an accumulation of evidence that Piwi proteins may be active in hematopoietic tissues. Thus, MIWI2 may have a role in hematopoietic stem and/or progenitor cell self-renewal and differentiation, and defects in MIWI2 may lead to abnormal hematopoiesis. MIWI2 mRNA can be detected in a mouse erythroblast cell line by RNA-seq, and shRNA-mediated knockdown of this mRNA causes the cells to take on characteristics of differentiated erythroid precursors. However, there are no detectable hematopoietic abnormalities in a MIWI2-deficient mouse model. While subtle, non-statistically significant changes were noted in the hematopoietic function of mice without a functional MIWI2 gene when compared to wild type mice, our results show that MIWI2 is not solely necessary for hematopoiesis within the normal life span of a mouse. |
| doi_str_mv | 10.1371/journal.pone.0082573 |
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MIWI2, one of the mouse Piwi-like proteins, is expressed in the prepachytene phase of spermatogenesis during the period of de novo methylation. Absence of this protein leads to meiotic defects and a progressive loss of germ cells. There is an accumulation of evidence that Piwi proteins may be active in hematopoietic tissues. Thus, MIWI2 may have a role in hematopoietic stem and/or progenitor cell self-renewal and differentiation, and defects in MIWI2 may lead to abnormal hematopoiesis. MIWI2 mRNA can be detected in a mouse erythroblast cell line by RNA-seq, and shRNA-mediated knockdown of this mRNA causes the cells to take on characteristics of differentiated erythroid precursors. However, there are no detectable hematopoietic abnormalities in a MIWI2-deficient mouse model. While subtle, non-statistically significant changes were noted in the hematopoietic function of mice without a functional MIWI2 gene when compared to wild type mice, our results show that MIWI2 is not solely necessary for hematopoiesis within the normal life span of a mouse.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0082573</identifier><identifier>PMID: 24376547</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abnormalities ; Acids ; Aging - pathology ; Animal tissues ; Animals ; Argonaute Proteins - deficiency ; Argonaute Proteins - metabolism ; Blood Cells - metabolism ; Cell cycle ; Cell Differentiation ; Cell Line, Tumor ; Cell self-renewal ; Children & youth ; Defects ; Differentiation (biology) ; DNA methylation ; Drosophila ; Erythroleukemia ; Gene Knockdown Techniques ; Genes ; Genomes ; Genomics ; Germ cells ; Hematopoiesis ; Hematopoietic stem cells ; Hemoglobins - metabolism ; House mouse ; Insects ; Leukemia, Erythroblastic, Acute - genetics ; Leukemia, Erythroblastic, Acute - pathology ; Life span ; Meiosis ; Methylation ; Mice ; Mice, Inbred C57BL ; mRNA ; Non-coding RNA ; Organ Specificity - genetics ; Progenitor cells ; Proteins ; Ribonucleic acid ; RNA ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA, Small Interfering - metabolism ; Rodents ; Sequence Analysis, RNA ; Spermatogenesis ; Spleen - metabolism ; Statistical analysis ; Stem cells ; Studies ; Whole-Body Irradiation</subject><ispartof>PloS one, 2013-12, Vol.8 (12), p.e82573-e82573</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Jacobs et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Jacobs et al 2013 Jacobs et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c593t-85e58ce55e6ff99a156bde403861de8db4ea106d83232afd2ddbeb95b874fff43</citedby><cites>FETCH-LOGICAL-c593t-85e58ce55e6ff99a156bde403861de8db4ea106d83232afd2ddbeb95b874fff43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871168/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871168/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23864,27922,27923,53789,53791,79370,79371</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24376547$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Suter, Catherine M.</contributor><creatorcontrib>Jacobs, James E</creatorcontrib><creatorcontrib>Wagner, Mark</creatorcontrib><creatorcontrib>Dhahbi, Joseph</creatorcontrib><creatorcontrib>Boffelli, Dario</creatorcontrib><creatorcontrib>Martin, David I K</creatorcontrib><title>Deficiency of MIWI2 (Piwil4) induces mouse erythroleukemia cell differentiation, but has no effect on hematopoiesis in vivo</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Piwi proteins and their small non-coding RNA partners are involved in the maintenance of stem cell character and genome integrity in the male germ cells of mammals. MIWI2, one of the mouse Piwi-like proteins, is expressed in the prepachytene phase of spermatogenesis during the period of de novo methylation. Absence of this protein leads to meiotic defects and a progressive loss of germ cells. There is an accumulation of evidence that Piwi proteins may be active in hematopoietic tissues. Thus, MIWI2 may have a role in hematopoietic stem and/or progenitor cell self-renewal and differentiation, and defects in MIWI2 may lead to abnormal hematopoiesis. MIWI2 mRNA can be detected in a mouse erythroblast cell line by RNA-seq, and shRNA-mediated knockdown of this mRNA causes the cells to take on characteristics of differentiated erythroid precursors. However, there are no detectable hematopoietic abnormalities in a MIWI2-deficient mouse model. While subtle, non-statistically significant changes were noted in the hematopoietic function of mice without a functional MIWI2 gene when compared to wild type mice, our results show that MIWI2 is not solely necessary for hematopoiesis within the normal life span of a mouse.</description><subject>Abnormalities</subject><subject>Acids</subject><subject>Aging - pathology</subject><subject>Animal tissues</subject><subject>Animals</subject><subject>Argonaute Proteins - deficiency</subject><subject>Argonaute Proteins - metabolism</subject><subject>Blood Cells - metabolism</subject><subject>Cell cycle</subject><subject>Cell Differentiation</subject><subject>Cell Line, Tumor</subject><subject>Cell self-renewal</subject><subject>Children & youth</subject><subject>Defects</subject><subject>Differentiation (biology)</subject><subject>DNA methylation</subject><subject>Drosophila</subject><subject>Erythroleukemia</subject><subject>Gene Knockdown Techniques</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Germ cells</subject><subject>Hematopoiesis</subject><subject>Hematopoietic stem cells</subject><subject>Hemoglobins - metabolism</subject><subject>House mouse</subject><subject>Insects</subject><subject>Leukemia, Erythroblastic, Acute - genetics</subject><subject>Leukemia, Erythroblastic, Acute - pathology</subject><subject>Life span</subject><subject>Meiosis</subject><subject>Methylation</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>mRNA</subject><subject>Non-coding RNA</subject><subject>Organ Specificity - genetics</subject><subject>Progenitor cells</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Small Interfering - metabolism</subject><subject>Rodents</subject><subject>Sequence Analysis, RNA</subject><subject>Spermatogenesis</subject><subject>Spleen - metabolism</subject><subject>Statistical analysis</subject><subject>Stem cells</subject><subject>Studies</subject><subject>Whole-Body Irradiation</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUk1vEzEQXSEQLYV_gMASlyKRYK_ttfeCVJWvSEVwAHG0vPY4cdhdp_ZuUMSfxyHbqkGVD7Zm3ryZN35F8ZzgOaGCvF2HMfa6nW9CD3OMZckFfVCckpqWs6rE9OGd90nxJKU1xpzKqnpcnJSMioozcVr8eQ_OGw-92aHg0JfFz0WJzr_5375lr5Hv7WggoS6MCRDE3bCKoYXxF3ReIwNti6x3DiL0g9eDD_0b1IwDWumE-oAgp8yAQo9W0OkhbIKH5FOmRVu_DU-LR063CZ5N91nx4-OH75efZ1dfPy0uL65mhtd0mEkOXBrgHCrn6loTXjUWGM5aiAVpGwaa4MpKWtJSO1ta20BT80YK5pxj9Kx4eeDdtCGpaW9JESYw57JiPCMWB4QNeq020Xc67lTQXv0LhLhUOg7etKAY1w3l2BKsK0YYkbXFFoC70uT-YDPXu6nb2HRgTV5N1O0R6XGm9yu1DFtFpSCkkpngfCKI4XqENKjOp_2udQ_5H_LcNRZYEiEy9NV_0PvVTailzgJ870Lua_ak6oIJSZiUjGbU_B5UPjZ_tskmcz7HjwrYocDEkFIEd6uRYLW36M0wam9RNVk0l724u5_bohtP0r9WT-Tj</recordid><startdate>20131223</startdate><enddate>20131223</enddate><creator>Jacobs, James E</creator><creator>Wagner, Mark</creator><creator>Dhahbi, Joseph</creator><creator>Boffelli, Dario</creator><creator>Martin, David I K</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131223</creationdate><title>Deficiency of MIWI2 (Piwil4) induces mouse erythroleukemia cell differentiation, but has no effect on hematopoiesis in vivo</title><author>Jacobs, James E ; Wagner, Mark ; Dhahbi, Joseph ; Boffelli, Dario ; Martin, David I K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c593t-85e58ce55e6ff99a156bde403861de8db4ea106d83232afd2ddbeb95b874fff43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Abnormalities</topic><topic>Acids</topic><topic>Aging - pathology</topic><topic>Animal tissues</topic><topic>Animals</topic><topic>Argonaute Proteins - deficiency</topic><topic>Argonaute Proteins - metabolism</topic><topic>Blood Cells - metabolism</topic><topic>Cell cycle</topic><topic>Cell Differentiation</topic><topic>Cell Line, Tumor</topic><topic>Cell self-renewal</topic><topic>Children & youth</topic><topic>Defects</topic><topic>Differentiation (biology)</topic><topic>DNA methylation</topic><topic>Drosophila</topic><topic>Erythroleukemia</topic><topic>Gene Knockdown Techniques</topic><topic>Genes</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Germ cells</topic><topic>Hematopoiesis</topic><topic>Hematopoietic stem cells</topic><topic>Hemoglobins - metabolism</topic><topic>House mouse</topic><topic>Insects</topic><topic>Leukemia, Erythroblastic, Acute - genetics</topic><topic>Leukemia, Erythroblastic, Acute - pathology</topic><topic>Life span</topic><topic>Meiosis</topic><topic>Methylation</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>mRNA</topic><topic>Non-coding RNA</topic><topic>Organ Specificity - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jacobs, James E</au><au>Wagner, Mark</au><au>Dhahbi, Joseph</au><au>Boffelli, Dario</au><au>Martin, David I K</au><au>Suter, Catherine M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deficiency of MIWI2 (Piwil4) induces mouse erythroleukemia cell differentiation, but has no effect on hematopoiesis in vivo</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-12-23</date><risdate>2013</risdate><volume>8</volume><issue>12</issue><spage>e82573</spage><epage>e82573</epage><pages>e82573-e82573</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Piwi proteins and their small non-coding RNA partners are involved in the maintenance of stem cell character and genome integrity in the male germ cells of mammals. MIWI2, one of the mouse Piwi-like proteins, is expressed in the prepachytene phase of spermatogenesis during the period of de novo methylation. Absence of this protein leads to meiotic defects and a progressive loss of germ cells. There is an accumulation of evidence that Piwi proteins may be active in hematopoietic tissues. Thus, MIWI2 may have a role in hematopoietic stem and/or progenitor cell self-renewal and differentiation, and defects in MIWI2 may lead to abnormal hematopoiesis. MIWI2 mRNA can be detected in a mouse erythroblast cell line by RNA-seq, and shRNA-mediated knockdown of this mRNA causes the cells to take on characteristics of differentiated erythroid precursors. However, there are no detectable hematopoietic abnormalities in a MIWI2-deficient mouse model. While subtle, non-statistically significant changes were noted in the hematopoietic function of mice without a functional MIWI2 gene when compared to wild type mice, our results show that MIWI2 is not solely necessary for hematopoiesis within the normal life span of a mouse.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24376547</pmid><doi>10.1371/journal.pone.0082573</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Abnormalities Acids Aging - pathology Animal tissues Animals Argonaute Proteins - deficiency Argonaute Proteins - metabolism Blood Cells - metabolism Cell cycle Cell Differentiation Cell Line, Tumor Cell self-renewal Children & youth Defects Differentiation (biology) DNA methylation Drosophila Erythroleukemia Gene Knockdown Techniques Genes Genomes Genomics Germ cells Hematopoiesis Hematopoietic stem cells Hemoglobins - metabolism House mouse Insects Leukemia, Erythroblastic, Acute - genetics Leukemia, Erythroblastic, Acute - pathology Life span Meiosis Methylation Mice Mice, Inbred C57BL mRNA Non-coding RNA Organ Specificity - genetics Progenitor cells Proteins Ribonucleic acid RNA RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Small Interfering - metabolism Rodents Sequence Analysis, RNA Spermatogenesis Spleen - metabolism Statistical analysis Stem cells Studies Whole-Body Irradiation |
| title | Deficiency of MIWI2 (Piwil4) induces mouse erythroleukemia cell differentiation, but has no effect on hematopoiesis in vivo |
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