A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserine
Aging of biological systems is controlled by various processes which have a potential impact on gene expression. Here we report a genome-wide transcriptome analysis of the fungal aging model Podospora anserina. Total RNA of three individuals of defined age were pooled and analyzed by SuperSAGE (seri...
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| Veröffentlicht in: | PloS one 2013-12, Vol.8 (12), p.e83109 |
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| creator | Philipp, Oliver Hamann, Andrea Servos, Jörg Werner, Alexandra Koch, Ina Osiewacz, Heinz D |
| description | Aging of biological systems is controlled by various processes which have a potential impact on gene expression. Here we report a genome-wide transcriptome analysis of the fungal aging model Podospora anserina. Total RNA of three individuals of defined age were pooled and analyzed by SuperSAGE (serial analysis of gene expression). A bioinformatics analysis identified different molecular pathways to be affected during aging. While the abundance of transcripts linked to ribosomes and to the proteasome quality control system were found to decrease during aging, those associated with autophagy increase, suggesting that autophagy may act as a compensatory quality control pathway. Transcript profiles associated with the energy metabolism including mitochondrial functions were identified to fluctuate during aging. Comparison of wild-type transcripts, which are continuously down-regulated during aging, with those down-regulated in the long-lived, copper-uptake mutant grisea, validated the relevance of age-related changes in cellular copper metabolism. Overall, we (i) present a unique age-related data set of a longitudinal study of the experimental aging model P. anserina which represents a reference resource for future investigations in a variety of organisms, (ii) suggest autophagy to be a key quality control pathway that becomes active once other pathways fail, and (iii) present testable predictions for subsequent experimental investigations. |
| doi_str_mv | 10.1371/journal.pone.0083109 |
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Here we report a genome-wide transcriptome analysis of the fungal aging model Podospora anserina. Total RNA of three individuals of defined age were pooled and analyzed by SuperSAGE (serial analysis of gene expression). A bioinformatics analysis identified different molecular pathways to be affected during aging. While the abundance of transcripts linked to ribosomes and to the proteasome quality control system were found to decrease during aging, those associated with autophagy increase, suggesting that autophagy may act as a compensatory quality control pathway. Transcript profiles associated with the energy metabolism including mitochondrial functions were identified to fluctuate during aging. Comparison of wild-type transcripts, which are continuously down-regulated during aging, with those down-regulated in the long-lived, copper-uptake mutant grisea, validated the relevance of age-related changes in cellular copper metabolism. Overall, we (i) present a unique age-related data set of a longitudinal study of the experimental aging model P. anserina which represents a reference resource for future investigations in a variety of organisms, (ii) suggest autophagy to be a key quality control pathway that becomes active once other pathways fail, and (iii) present testable predictions for subsequent experimental investigations.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0083109</identifier><identifier>PMID: 24376646</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Active control ; Age ; Aging ; Analysis ; Autophagy ; Bioinformatics ; Cell death ; Computer science ; Control systems ; Copper ; Correlation analysis ; Developmental biology ; Disease ; Energy metabolism ; Fungi ; Gene expression ; Genes ; Genetic engineering ; Genomes ; Genomics ; Kinases ; Metabolism ; Mitochondria ; Neurodegeneration ; Phagocytosis ; Physiological aspects ; Proteasomes ; Quality control ; Quality management ; Ribonucleic acid ; Ribosomes ; RNA ; Serial analysis of gene expression ; Transcription</subject><ispartof>PloS one, 2013-12, Vol.8 (12), p.e83109</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Philipp et al. 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Here we report a genome-wide transcriptome analysis of the fungal aging model Podospora anserina. Total RNA of three individuals of defined age were pooled and analyzed by SuperSAGE (serial analysis of gene expression). A bioinformatics analysis identified different molecular pathways to be affected during aging. While the abundance of transcripts linked to ribosomes and to the proteasome quality control system were found to decrease during aging, those associated with autophagy increase, suggesting that autophagy may act as a compensatory quality control pathway. Transcript profiles associated with the energy metabolism including mitochondrial functions were identified to fluctuate during aging. Comparison of wild-type transcripts, which are continuously down-regulated during aging, with those down-regulated in the long-lived, copper-uptake mutant grisea, validated the relevance of age-related changes in cellular copper metabolism. 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Here we report a genome-wide transcriptome analysis of the fungal aging model Podospora anserina. Total RNA of three individuals of defined age were pooled and analyzed by SuperSAGE (serial analysis of gene expression). A bioinformatics analysis identified different molecular pathways to be affected during aging. While the abundance of transcripts linked to ribosomes and to the proteasome quality control system were found to decrease during aging, those associated with autophagy increase, suggesting that autophagy may act as a compensatory quality control pathway. Transcript profiles associated with the energy metabolism including mitochondrial functions were identified to fluctuate during aging. Comparison of wild-type transcripts, which are continuously down-regulated during aging, with those down-regulated in the long-lived, copper-uptake mutant grisea, validated the relevance of age-related changes in cellular copper metabolism. 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| subjects | Active control Age Aging Analysis Autophagy Bioinformatics Cell death Computer science Control systems Copper Correlation analysis Developmental biology Disease Energy metabolism Fungi Gene expression Genes Genetic engineering Genomes Genomics Kinases Metabolism Mitochondria Neurodegeneration Phagocytosis Physiological aspects Proteasomes Quality control Quality management Ribonucleic acid Ribosomes RNA Serial analysis of gene expression Transcription |
| title | A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserine |
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