Urinary nucleosides as biomarkers of breast, colon, lung, and gastric cancer in Taiwanese
Urinary nucleosides are associated with many types of cancer. In this study, six targeted urinary nucleosides, namely adenosine, cytidine, 3-methylcytidine, 1-methyladenosine, inosine, and 2-deoxyguanosine, were chosen to evaluate their role as biomarkers of four different types of cancer: lung canc...
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| creator | Hsu, Wei-Yi Chen, Chao-Jung Huang, Yu-Chuen Tsai, Fuu-Jen Jeng, Long-Bin Lai, Chien-Chen |
| description | Urinary nucleosides are associated with many types of cancer. In this study, six targeted urinary nucleosides, namely adenosine, cytidine, 3-methylcytidine, 1-methyladenosine, inosine, and 2-deoxyguanosine, were chosen to evaluate their role as biomarkers of four different types of cancer: lung cancer, gastric cancer, colon cancer, and breast cancer. Urine samples were purified using solid-phase extraction (SPE) and then analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The Mann-Whitney U test and Principal Component Analysis (PCA) were used to compare differences in urinary nucleosides between patients with one of four types of cancer and healthy controls. The diagnostic sensitivity of single nucleosides for different types of cancer ranged from 14% to 69%. In contrast, the diagnostic sensitivity of a set of six nucleosides ranged from 37% to 69%. The false-positive identification rate associated with the set of six nucleosides in urine was less than 2% compared with that of less than 5% for a single nucleoside. Furthermore, combining the set of six urinary nucleosides with carcinoembryonic antigen improved the diagnostic sensitivity for colon cancer. In summary, the study show that a set of six targeted nucleosides is a good diagnostic marker for breast and colon cancers but not for lung and gastric cancers. |
| doi_str_mv | 10.1371/journal.pone.0081701 |
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In this study, six targeted urinary nucleosides, namely adenosine, cytidine, 3-methylcytidine, 1-methyladenosine, inosine, and 2-deoxyguanosine, were chosen to evaluate their role as biomarkers of four different types of cancer: lung cancer, gastric cancer, colon cancer, and breast cancer. Urine samples were purified using solid-phase extraction (SPE) and then analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The Mann-Whitney U test and Principal Component Analysis (PCA) were used to compare differences in urinary nucleosides between patients with one of four types of cancer and healthy controls. The diagnostic sensitivity of single nucleosides for different types of cancer ranged from 14% to 69%. In contrast, the diagnostic sensitivity of a set of six nucleosides ranged from 37% to 69%. The false-positive identification rate associated with the set of six nucleosides in urine was less than 2% compared with that of less than 5% for a single nucleoside. Furthermore, combining the set of six urinary nucleosides with carcinoembryonic antigen improved the diagnostic sensitivity for colon cancer. In summary, the study show that a set of six targeted nucleosides is a good diagnostic marker for breast and colon cancers but not for lung and gastric cancers.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0081701</identifier><identifier>PMID: 24367489</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adenosine ; Analysis ; Biological markers ; Biomarkers ; Biomarkers, Tumor - urine ; Breast cancer ; Breast Neoplasms - urine ; Cancer ; Capillary electrophoresis ; Carcinoembryonic antigen ; Chromatography ; Colon ; Colon cancer ; Colonic Neoplasms - urine ; Colorectal cancer ; Deoxyguanosine ; Diagnosis ; Diagnostic systems ; Gastric cancer ; High performance liquid chromatography ; Hospitals ; Humans ; Ions ; Liquid chromatography ; Lung cancer ; Lung diseases ; Lung Neoplasms - urine ; Mass spectrometry ; Mass spectroscopy ; Medical diagnosis ; Medical research ; Nucleosides ; Nucleosides - urine ; Principal components analysis ; Scientific imaging ; Sensitivity ; Stomach cancer ; Stomach Neoplasms - urine ; Studies ; Taiwan ; Transfer RNA ; Urine</subject><ispartof>PloS one, 2013-12, Vol.8 (12), p.e81701-e81701</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Hsu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Hsu et al 2013 Hsu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c688t-dd7b732d3bb872bdbc0e8958926d88d36796f97ef2f5bc46439549bce9451b063</citedby><cites>FETCH-LOGICAL-c688t-dd7b732d3bb872bdbc0e8958926d88d36796f97ef2f5bc46439549bce9451b063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868621/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868621/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24367489$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Aziz, Syed A.</contributor><creatorcontrib>Hsu, Wei-Yi</creatorcontrib><creatorcontrib>Chen, Chao-Jung</creatorcontrib><creatorcontrib>Huang, Yu-Chuen</creatorcontrib><creatorcontrib>Tsai, Fuu-Jen</creatorcontrib><creatorcontrib>Jeng, Long-Bin</creatorcontrib><creatorcontrib>Lai, Chien-Chen</creatorcontrib><title>Urinary nucleosides as biomarkers of breast, colon, lung, and gastric cancer in Taiwanese</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Urinary nucleosides are associated with many types of cancer. In this study, six targeted urinary nucleosides, namely adenosine, cytidine, 3-methylcytidine, 1-methyladenosine, inosine, and 2-deoxyguanosine, were chosen to evaluate their role as biomarkers of four different types of cancer: lung cancer, gastric cancer, colon cancer, and breast cancer. Urine samples were purified using solid-phase extraction (SPE) and then analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The Mann-Whitney U test and Principal Component Analysis (PCA) were used to compare differences in urinary nucleosides between patients with one of four types of cancer and healthy controls. The diagnostic sensitivity of single nucleosides for different types of cancer ranged from 14% to 69%. In contrast, the diagnostic sensitivity of a set of six nucleosides ranged from 37% to 69%. The false-positive identification rate associated with the set of six nucleosides in urine was less than 2% compared with that of less than 5% for a single nucleoside. Furthermore, combining the set of six urinary nucleosides with carcinoembryonic antigen improved the diagnostic sensitivity for colon cancer. In summary, the study show that a set of six targeted nucleosides is a good diagnostic marker for breast and colon cancers but not for lung and gastric cancers.</description><subject>Adenosine</subject><subject>Analysis</subject><subject>Biological markers</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - urine</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - urine</subject><subject>Cancer</subject><subject>Capillary electrophoresis</subject><subject>Carcinoembryonic antigen</subject><subject>Chromatography</subject><subject>Colon</subject><subject>Colon cancer</subject><subject>Colonic Neoplasms - urine</subject><subject>Colorectal cancer</subject><subject>Deoxyguanosine</subject><subject>Diagnosis</subject><subject>Diagnostic systems</subject><subject>Gastric cancer</subject><subject>High performance liquid chromatography</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Ions</subject><subject>Liquid chromatography</subject><subject>Lung cancer</subject><subject>Lung diseases</subject><subject>Lung Neoplasms - urine</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical diagnosis</subject><subject>Medical research</subject><subject>Nucleosides</subject><subject>Nucleosides - urine</subject><subject>Principal components analysis</subject><subject>Scientific imaging</subject><subject>Sensitivity</subject><subject>Stomach cancer</subject><subject>Stomach Neoplasms - urine</subject><subject>Studies</subject><subject>Taiwan</subject><subject>Transfer RNA</subject><subject>Urine</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11rFDEUhgdRbK3-A9GAIAq76-RjZpIboRQ_FgoFbQWvQr5mNms22SYzav-9GXdadqUXMhcZTp7z5pw3OUXxHJYLiBv4bh2G6IVbbIM3i7KksCnhg-IYMozmNSrxw73_o-JJSuuyrDCt68fFESK4bghlx8X3q2i9iDfAD8qZkKw2CYgEpA0bEX-YmEBogYxGpH4GVHDBz4AbfDcDwmvQ5XC0CijhlYnAenAp7C_hTTJPi0etcMk8m9aT4urjh8uzz_Pzi0_Ls9Pzuaop7edaN7LBSGMpaYOklqo0lFWUoVpTqnOdrG5ZY1rUVlKRmmBWESaVYaSCsqzxSfFyp7t1IfHJlcQhqVl2hDKSieWO0EGs-Tba3NkND8Lyv4EQOy5ib3P_XLetxNDkCiQkKpchkYa4JQzCRlUGZ63302mD3BitjO-jcAeihzverngXfvLsPK0RzAJvJoEYrgeTer6xSRnnsmlhGOtmZYMYasbOXv2D3t_dRHUiN2B9G_K5ahTlp6ShKAvSUWtxD5U_bTZW5SfU2hw_SHh7kJCZ3vzuOzGkxJdfv_w_e_HtkH29x66McP0qBTf0Nvh0CJIdqGJIKZr2zmRY8nECbt3g4wTwaQJy2ov9C7pLun3y-A8OwgBH</recordid><startdate>20131219</startdate><enddate>20131219</enddate><creator>Hsu, Wei-Yi</creator><creator>Chen, Chao-Jung</creator><creator>Huang, Yu-Chuen</creator><creator>Tsai, Fuu-Jen</creator><creator>Jeng, Long-Bin</creator><creator>Lai, Chien-Chen</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131219</creationdate><title>Urinary nucleosides as biomarkers of breast, colon, lung, and gastric cancer in Taiwanese</title><author>Hsu, Wei-Yi ; Chen, Chao-Jung ; Huang, Yu-Chuen ; Tsai, Fuu-Jen ; Jeng, Long-Bin ; Lai, Chien-Chen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c688t-dd7b732d3bb872bdbc0e8958926d88d36796f97ef2f5bc46439549bce9451b063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adenosine</topic><topic>Analysis</topic><topic>Biological markers</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - urine</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - urine</topic><topic>Cancer</topic><topic>Capillary electrophoresis</topic><topic>Carcinoembryonic antigen</topic><topic>Chromatography</topic><topic>Colon</topic><topic>Colon cancer</topic><topic>Colonic Neoplasms - urine</topic><topic>Colorectal cancer</topic><topic>Deoxyguanosine</topic><topic>Diagnosis</topic><topic>Diagnostic systems</topic><topic>Gastric cancer</topic><topic>High performance liquid chromatography</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Ions</topic><topic>Liquid chromatography</topic><topic>Lung cancer</topic><topic>Lung diseases</topic><topic>Lung Neoplasms - urine</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medical diagnosis</topic><topic>Medical research</topic><topic>Nucleosides</topic><topic>Nucleosides - urine</topic><topic>Principal components analysis</topic><topic>Scientific imaging</topic><topic>Sensitivity</topic><topic>Stomach cancer</topic><topic>Stomach Neoplasms - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hsu, Wei-Yi</au><au>Chen, Chao-Jung</au><au>Huang, Yu-Chuen</au><au>Tsai, Fuu-Jen</au><au>Jeng, Long-Bin</au><au>Lai, Chien-Chen</au><au>Aziz, Syed A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Urinary nucleosides as biomarkers of breast, colon, lung, and gastric cancer in Taiwanese</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-12-19</date><risdate>2013</risdate><volume>8</volume><issue>12</issue><spage>e81701</spage><epage>e81701</epage><pages>e81701-e81701</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Urinary nucleosides are associated with many types of cancer. In this study, six targeted urinary nucleosides, namely adenosine, cytidine, 3-methylcytidine, 1-methyladenosine, inosine, and 2-deoxyguanosine, were chosen to evaluate their role as biomarkers of four different types of cancer: lung cancer, gastric cancer, colon cancer, and breast cancer. Urine samples were purified using solid-phase extraction (SPE) and then analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The Mann-Whitney U test and Principal Component Analysis (PCA) were used to compare differences in urinary nucleosides between patients with one of four types of cancer and healthy controls. The diagnostic sensitivity of single nucleosides for different types of cancer ranged from 14% to 69%. In contrast, the diagnostic sensitivity of a set of six nucleosides ranged from 37% to 69%. The false-positive identification rate associated with the set of six nucleosides in urine was less than 2% compared with that of less than 5% for a single nucleoside. Furthermore, combining the set of six urinary nucleosides with carcinoembryonic antigen improved the diagnostic sensitivity for colon cancer. In summary, the study show that a set of six targeted nucleosides is a good diagnostic marker for breast and colon cancers but not for lung and gastric cancers.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24367489</pmid><doi>10.1371/journal.pone.0081701</doi><tpages>e81701</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenosine Analysis Biological markers Biomarkers Biomarkers, Tumor - urine Breast cancer Breast Neoplasms - urine Cancer Capillary electrophoresis Carcinoembryonic antigen Chromatography Colon Colon cancer Colonic Neoplasms - urine Colorectal cancer Deoxyguanosine Diagnosis Diagnostic systems Gastric cancer High performance liquid chromatography Hospitals Humans Ions Liquid chromatography Lung cancer Lung diseases Lung Neoplasms - urine Mass spectrometry Mass spectroscopy Medical diagnosis Medical research Nucleosides Nucleosides - urine Principal components analysis Scientific imaging Sensitivity Stomach cancer Stomach Neoplasms - urine Studies Taiwan Transfer RNA Urine |
| title | Urinary nucleosides as biomarkers of breast, colon, lung, and gastric cancer in Taiwanese |
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