Characterization of neuronal populations in the human trigeminal ganglion and their association with latent herpes simplex virus-1 infection

Following primary infection Herpes simplex virus-1 (HSV-1) establishes lifelong latency in the neurons of human sensory ganglia. Upon reactivation HSV-1 can cause neurological diseases such as facial palsy, vestibular neuritis or encephalitis. Certain populations of sensory neurons have been shown t...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:PloS one 2013-12, Vol.8 (12), p.e83603-e83603
Hauptverfasser: Flowerdew, Sarah E, Wick, Desiree, Himmelein, Susanne, Horn, Anja K E, Sinicina, Inga, Strupp, Michael, Brandt, Thomas, Theil, Diethilde, Hüfner, Katharina
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page e83603
container_issue 12
container_start_page e83603
container_title PloS one
container_volume 8
creator Flowerdew, Sarah E
Wick, Desiree
Himmelein, Susanne
Horn, Anja K E
Sinicina, Inga
Strupp, Michael
Brandt, Thomas
Theil, Diethilde
Hüfner, Katharina
description Following primary infection Herpes simplex virus-1 (HSV-1) establishes lifelong latency in the neurons of human sensory ganglia. Upon reactivation HSV-1 can cause neurological diseases such as facial palsy, vestibular neuritis or encephalitis. Certain populations of sensory neurons have been shown to be more susceptible to latent infection in the animal model, but this has not been addressed in human tissue. In the present study, trigeminal ganglion (TG) neurons expressing six neuronal marker proteins were characterized, based on staining with antibodies against the GDNF family ligand receptor Ret, the high-affinity nerve growth factor receptor TrkA, neuronal nitric oxide synthase (nNOS), the antibody RT97 against 200 kDa neurofilament, calcitonin gene-related peptide and peripherin. The frequencies of marker-positive neurons and their average neuronal sizes were assessed, with TrkA-positive (61.82%) neurons being the most abundant, and Ret-positive (26.93%) the least prevalent. Neurons positive with the antibody RT97 (1253 µm(2)) were the largest, and those stained against peripherin (884 µm(2)) were the smallest. Dual immunofluorescence revealed at least a 4.5% overlap for every tested marker combination, with overlap for the combinations TrkA/Ret, TrkA/RT97 and Ret/nNOS lower, and the overlap between Ret/CGRP being higher than would be expected by chance. With respect to latent HSV-1 infection, latency associated transcripts (LAT) were detected using in situ hybridization (ISH) in neurons expressing each of the marker proteins. In contrast to the mouse model, co-localization with neuronal markers Ret or CGRP mirrored the magnitude of these neuron populations, whereas for the other four neuronal markers fewer marker-positive cells were also LAT-ISH+. Ret and CGRP are both known to label neurons related to pain signaling.
doi_str_mv 10.1371/journal.pone.0083603
format Article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1469701698</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478214990</galeid><doaj_id>oai_doaj_org_article_381fa89e65f348aebd7058f8449e0b03</doaj_id><sourcerecordid>A478214990</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-7420f5b6f736f0118c6ee76f7842c18240da6f5947ed9eacc5af524eddbb13a3</originalsourceid><addsrcrecordid>eNqNk92K1DAUgIso7rr6BqIFQfRixqRJ0_RmYRn8GVhY0MXbkKYnbYa2qUm7rj6DD206012mshfSiyan3_nSnORE0UuM1phk-MPOjq6Tzbq3HawR4oQh8ig6xTlJVixB5PHR-CR65v0OoZRwxp5GJwklLAv8afRnU0sn1QDO_JaDsV1sddzB6Gxwx73tx2Yf9rHp4qGGuB5bGUbOVNCaialkVzVTouzKiTAult5bZQ66n2ao4-CAbohrcD342Ju2b-A2vjFu9CsczBrUBD-PnmjZeHgxv8-i608frzdfVpdXn7ebi8uVYnkyrDKaIJ0WTGeEaYQxVwwgC1NOE4V5QlEpmU5zmkGZg1QqlTpNKJRlUWAiyVn0-qDtG-vFXEcvMGV5hjDLeSC2B6K0cid6Z1rpfgkrjdgHrKuEdINRDQjCsZY8B5ZqQrmEosxQyjWnNAdUIBJc5_NqY9FCqUIhnGwW0uWXztSisjfBzHia4yB4Nwuc_TGCH0RrvIKmkR3YcfrvHGVJTkkS0Df_oA_vbqYqGTYQqm_DumqSigua8SQIcxSo9QNUeMpw8CpcOm1CfJHwfpEQmAFuh0qO3ovtt6__z159X7Jvj9gaZDPU3jbj_louQXoAlbPeO9D3RcZITD1zVw0x9YyYeyakvTo-oPukuyYhfwGY-hTO</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1469701698</pqid></control><display><type>article</type><title>Characterization of neuronal populations in the human trigeminal ganglion and their association with latent herpes simplex virus-1 infection</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Flowerdew, Sarah E ; Wick, Desiree ; Himmelein, Susanne ; Horn, Anja K E ; Sinicina, Inga ; Strupp, Michael ; Brandt, Thomas ; Theil, Diethilde ; Hüfner, Katharina</creator><contributor>Efstathiou, Stacey</contributor><creatorcontrib>Flowerdew, Sarah E ; Wick, Desiree ; Himmelein, Susanne ; Horn, Anja K E ; Sinicina, Inga ; Strupp, Michael ; Brandt, Thomas ; Theil, Diethilde ; Hüfner, Katharina ; Efstathiou, Stacey</creatorcontrib><description>Following primary infection Herpes simplex virus-1 (HSV-1) establishes lifelong latency in the neurons of human sensory ganglia. Upon reactivation HSV-1 can cause neurological diseases such as facial palsy, vestibular neuritis or encephalitis. Certain populations of sensory neurons have been shown to be more susceptible to latent infection in the animal model, but this has not been addressed in human tissue. In the present study, trigeminal ganglion (TG) neurons expressing six neuronal marker proteins were characterized, based on staining with antibodies against the GDNF family ligand receptor Ret, the high-affinity nerve growth factor receptor TrkA, neuronal nitric oxide synthase (nNOS), the antibody RT97 against 200 kDa neurofilament, calcitonin gene-related peptide and peripherin. The frequencies of marker-positive neurons and their average neuronal sizes were assessed, with TrkA-positive (61.82%) neurons being the most abundant, and Ret-positive (26.93%) the least prevalent. Neurons positive with the antibody RT97 (1253 µm(2)) were the largest, and those stained against peripherin (884 µm(2)) were the smallest. Dual immunofluorescence revealed at least a 4.5% overlap for every tested marker combination, with overlap for the combinations TrkA/Ret, TrkA/RT97 and Ret/nNOS lower, and the overlap between Ret/CGRP being higher than would be expected by chance. With respect to latent HSV-1 infection, latency associated transcripts (LAT) were detected using in situ hybridization (ISH) in neurons expressing each of the marker proteins. In contrast to the mouse model, co-localization with neuronal markers Ret or CGRP mirrored the magnitude of these neuron populations, whereas for the other four neuronal markers fewer marker-positive cells were also LAT-ISH+. Ret and CGRP are both known to label neurons related to pain signaling.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0083603</identifier><identifier>PMID: 24367603</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Activation ; Adult ; Aged ; Animals ; Antibodies ; Biomarkers ; Biomarkers - metabolism ; Calcitonin ; Calcitonin gene-related peptide ; Cell Size ; Deoxyribonucleic acid ; Diseases ; DNA ; Encephalitis ; Ethics ; Female ; Ganglia ; Ganglion cysts ; Gene Expression Regulation ; Glial cell line-derived neurotrophic factor ; Growth factors ; Health aspects ; Herpes simplex ; Herpes simplex virus ; Herpes viruses ; Herpesvirus 1, Human - metabolism ; Herpesvirus 1, Human - physiology ; Humans ; Immunofluorescence ; Infections ; Intermediate filament proteins ; Latency ; Latent infection ; Legal medicine ; Localization ; Male ; Middle Aged ; Nerve growth factor ; Neuritis ; Neurological diseases ; Neurology ; Neurons ; Neurons - cytology ; Neurons - virology ; Nitric oxide ; Nitric-oxide synthase ; Pain ; Paralysis ; Peripherin ; Populations ; Protein Transport ; Proteins ; Risk factors ; Sensory neurons ; Signaling ; Trigeminal ganglion ; Trigeminal Ganglion - cytology ; TrkA protein ; TrkA receptors ; Vestibular system ; Viral Proteins - genetics ; Viral Proteins - metabolism ; Viruses ; Young Adult</subject><ispartof>PloS one, 2013-12, Vol.8 (12), p.e83603-e83603</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Flowerdew et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Flowerdew et al 2013 Flowerdew et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-7420f5b6f736f0118c6ee76f7842c18240da6f5947ed9eacc5af524eddbb13a3</citedby><cites>FETCH-LOGICAL-c692t-7420f5b6f736f0118c6ee76f7842c18240da6f5947ed9eacc5af524eddbb13a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868591/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868591/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24367603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Efstathiou, Stacey</contributor><creatorcontrib>Flowerdew, Sarah E</creatorcontrib><creatorcontrib>Wick, Desiree</creatorcontrib><creatorcontrib>Himmelein, Susanne</creatorcontrib><creatorcontrib>Horn, Anja K E</creatorcontrib><creatorcontrib>Sinicina, Inga</creatorcontrib><creatorcontrib>Strupp, Michael</creatorcontrib><creatorcontrib>Brandt, Thomas</creatorcontrib><creatorcontrib>Theil, Diethilde</creatorcontrib><creatorcontrib>Hüfner, Katharina</creatorcontrib><title>Characterization of neuronal populations in the human trigeminal ganglion and their association with latent herpes simplex virus-1 infection</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Following primary infection Herpes simplex virus-1 (HSV-1) establishes lifelong latency in the neurons of human sensory ganglia. Upon reactivation HSV-1 can cause neurological diseases such as facial palsy, vestibular neuritis or encephalitis. Certain populations of sensory neurons have been shown to be more susceptible to latent infection in the animal model, but this has not been addressed in human tissue. In the present study, trigeminal ganglion (TG) neurons expressing six neuronal marker proteins were characterized, based on staining with antibodies against the GDNF family ligand receptor Ret, the high-affinity nerve growth factor receptor TrkA, neuronal nitric oxide synthase (nNOS), the antibody RT97 against 200 kDa neurofilament, calcitonin gene-related peptide and peripherin. The frequencies of marker-positive neurons and their average neuronal sizes were assessed, with TrkA-positive (61.82%) neurons being the most abundant, and Ret-positive (26.93%) the least prevalent. Neurons positive with the antibody RT97 (1253 µm(2)) were the largest, and those stained against peripherin (884 µm(2)) were the smallest. Dual immunofluorescence revealed at least a 4.5% overlap for every tested marker combination, with overlap for the combinations TrkA/Ret, TrkA/RT97 and Ret/nNOS lower, and the overlap between Ret/CGRP being higher than would be expected by chance. With respect to latent HSV-1 infection, latency associated transcripts (LAT) were detected using in situ hybridization (ISH) in neurons expressing each of the marker proteins. In contrast to the mouse model, co-localization with neuronal markers Ret or CGRP mirrored the magnitude of these neuron populations, whereas for the other four neuronal markers fewer marker-positive cells were also LAT-ISH+. Ret and CGRP are both known to label neurons related to pain signaling.</description><subject>Activation</subject><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Biomarkers</subject><subject>Biomarkers - metabolism</subject><subject>Calcitonin</subject><subject>Calcitonin gene-related peptide</subject><subject>Cell Size</subject><subject>Deoxyribonucleic acid</subject><subject>Diseases</subject><subject>DNA</subject><subject>Encephalitis</subject><subject>Ethics</subject><subject>Female</subject><subject>Ganglia</subject><subject>Ganglion cysts</subject><subject>Gene Expression Regulation</subject><subject>Glial cell line-derived neurotrophic factor</subject><subject>Growth factors</subject><subject>Health aspects</subject><subject>Herpes simplex</subject><subject>Herpes simplex virus</subject><subject>Herpes viruses</subject><subject>Herpesvirus 1, Human - metabolism</subject><subject>Herpesvirus 1, Human - physiology</subject><subject>Humans</subject><subject>Immunofluorescence</subject><subject>Infections</subject><subject>Intermediate filament proteins</subject><subject>Latency</subject><subject>Latent infection</subject><subject>Legal medicine</subject><subject>Localization</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Nerve growth factor</subject><subject>Neuritis</subject><subject>Neurological diseases</subject><subject>Neurology</subject><subject>Neurons</subject><subject>Neurons - cytology</subject><subject>Neurons - virology</subject><subject>Nitric oxide</subject><subject>Nitric-oxide synthase</subject><subject>Pain</subject><subject>Paralysis</subject><subject>Peripherin</subject><subject>Populations</subject><subject>Protein Transport</subject><subject>Proteins</subject><subject>Risk factors</subject><subject>Sensory neurons</subject><subject>Signaling</subject><subject>Trigeminal ganglion</subject><subject>Trigeminal Ganglion - cytology</subject><subject>TrkA protein</subject><subject>TrkA receptors</subject><subject>Vestibular system</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - metabolism</subject><subject>Viruses</subject><subject>Young Adult</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk92K1DAUgIso7rr6BqIFQfRixqRJ0_RmYRn8GVhY0MXbkKYnbYa2qUm7rj6DD206012mshfSiyan3_nSnORE0UuM1phk-MPOjq6Tzbq3HawR4oQh8ig6xTlJVixB5PHR-CR65v0OoZRwxp5GJwklLAv8afRnU0sn1QDO_JaDsV1sddzB6Gxwx73tx2Yf9rHp4qGGuB5bGUbOVNCaialkVzVTouzKiTAult5bZQ66n2ao4-CAbohrcD342Ju2b-A2vjFu9CsczBrUBD-PnmjZeHgxv8-i608frzdfVpdXn7ebi8uVYnkyrDKaIJ0WTGeEaYQxVwwgC1NOE4V5QlEpmU5zmkGZg1QqlTpNKJRlUWAiyVn0-qDtG-vFXEcvMGV5hjDLeSC2B6K0cid6Z1rpfgkrjdgHrKuEdINRDQjCsZY8B5ZqQrmEosxQyjWnNAdUIBJc5_NqY9FCqUIhnGwW0uWXztSisjfBzHia4yB4Nwuc_TGCH0RrvIKmkR3YcfrvHGVJTkkS0Df_oA_vbqYqGTYQqm_DumqSigua8SQIcxSo9QNUeMpw8CpcOm1CfJHwfpEQmAFuh0qO3ovtt6__z159X7Jvj9gaZDPU3jbj_louQXoAlbPeO9D3RcZITD1zVw0x9YyYeyakvTo-oPukuyYhfwGY-hTO</recordid><startdate>20131219</startdate><enddate>20131219</enddate><creator>Flowerdew, Sarah E</creator><creator>Wick, Desiree</creator><creator>Himmelein, Susanne</creator><creator>Horn, Anja K E</creator><creator>Sinicina, Inga</creator><creator>Strupp, Michael</creator><creator>Brandt, Thomas</creator><creator>Theil, Diethilde</creator><creator>Hüfner, Katharina</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131219</creationdate><title>Characterization of neuronal populations in the human trigeminal ganglion and their association with latent herpes simplex virus-1 infection</title><author>Flowerdew, Sarah E ; Wick, Desiree ; Himmelein, Susanne ; Horn, Anja K E ; Sinicina, Inga ; Strupp, Michael ; Brandt, Thomas ; Theil, Diethilde ; Hüfner, Katharina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-7420f5b6f736f0118c6ee76f7842c18240da6f5947ed9eacc5af524eddbb13a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Activation</topic><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Biomarkers</topic><topic>Biomarkers - metabolism</topic><topic>Calcitonin</topic><topic>Calcitonin gene-related peptide</topic><topic>Cell Size</topic><topic>Deoxyribonucleic acid</topic><topic>Diseases</topic><topic>DNA</topic><topic>Encephalitis</topic><topic>Ethics</topic><topic>Female</topic><topic>Ganglia</topic><topic>Ganglion cysts</topic><topic>Gene Expression Regulation</topic><topic>Glial cell line-derived neurotrophic factor</topic><topic>Growth factors</topic><topic>Health aspects</topic><topic>Herpes simplex</topic><topic>Herpes simplex virus</topic><topic>Herpes viruses</topic><topic>Herpesvirus 1, Human - metabolism</topic><topic>Herpesvirus 1, Human - physiology</topic><topic>Humans</topic><topic>Immunofluorescence</topic><topic>Infections</topic><topic>Intermediate filament proteins</topic><topic>Latency</topic><topic>Latent infection</topic><topic>Legal medicine</topic><topic>Localization</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Nerve growth factor</topic><topic>Neuritis</topic><topic>Neurological diseases</topic><topic>Neurology</topic><topic>Neurons</topic><topic>Neurons - cytology</topic><topic>Neurons - virology</topic><topic>Nitric oxide</topic><topic>Nitric-oxide synthase</topic><topic>Pain</topic><topic>Paralysis</topic><topic>Peripherin</topic><topic>Populations</topic><topic>Protein Transport</topic><topic>Proteins</topic><topic>Risk factors</topic><topic>Sensory neurons</topic><topic>Signaling</topic><topic>Trigeminal ganglion</topic><topic>Trigeminal Ganglion - cytology</topic><topic>TrkA protein</topic><topic>TrkA receptors</topic><topic>Vestibular system</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - metabolism</topic><topic>Viruses</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Flowerdew, Sarah E</creatorcontrib><creatorcontrib>Wick, Desiree</creatorcontrib><creatorcontrib>Himmelein, Susanne</creatorcontrib><creatorcontrib>Horn, Anja K E</creatorcontrib><creatorcontrib>Sinicina, Inga</creatorcontrib><creatorcontrib>Strupp, Michael</creatorcontrib><creatorcontrib>Brandt, Thomas</creatorcontrib><creatorcontrib>Theil, Diethilde</creatorcontrib><creatorcontrib>Hüfner, Katharina</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Flowerdew, Sarah E</au><au>Wick, Desiree</au><au>Himmelein, Susanne</au><au>Horn, Anja K E</au><au>Sinicina, Inga</au><au>Strupp, Michael</au><au>Brandt, Thomas</au><au>Theil, Diethilde</au><au>Hüfner, Katharina</au><au>Efstathiou, Stacey</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of neuronal populations in the human trigeminal ganglion and their association with latent herpes simplex virus-1 infection</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-12-19</date><risdate>2013</risdate><volume>8</volume><issue>12</issue><spage>e83603</spage><epage>e83603</epage><pages>e83603-e83603</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Following primary infection Herpes simplex virus-1 (HSV-1) establishes lifelong latency in the neurons of human sensory ganglia. Upon reactivation HSV-1 can cause neurological diseases such as facial palsy, vestibular neuritis or encephalitis. Certain populations of sensory neurons have been shown to be more susceptible to latent infection in the animal model, but this has not been addressed in human tissue. In the present study, trigeminal ganglion (TG) neurons expressing six neuronal marker proteins were characterized, based on staining with antibodies against the GDNF family ligand receptor Ret, the high-affinity nerve growth factor receptor TrkA, neuronal nitric oxide synthase (nNOS), the antibody RT97 against 200 kDa neurofilament, calcitonin gene-related peptide and peripherin. The frequencies of marker-positive neurons and their average neuronal sizes were assessed, with TrkA-positive (61.82%) neurons being the most abundant, and Ret-positive (26.93%) the least prevalent. Neurons positive with the antibody RT97 (1253 µm(2)) were the largest, and those stained against peripherin (884 µm(2)) were the smallest. Dual immunofluorescence revealed at least a 4.5% overlap for every tested marker combination, with overlap for the combinations TrkA/Ret, TrkA/RT97 and Ret/nNOS lower, and the overlap between Ret/CGRP being higher than would be expected by chance. With respect to latent HSV-1 infection, latency associated transcripts (LAT) were detected using in situ hybridization (ISH) in neurons expressing each of the marker proteins. In contrast to the mouse model, co-localization with neuronal markers Ret or CGRP mirrored the magnitude of these neuron populations, whereas for the other four neuronal markers fewer marker-positive cells were also LAT-ISH+. Ret and CGRP are both known to label neurons related to pain signaling.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24367603</pmid><doi>10.1371/journal.pone.0083603</doi><tpages>e83603</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2013-12, Vol.8 (12), p.e83603-e83603
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1469701698
source MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry
subjects Activation
Adult
Aged
Animals
Antibodies
Biomarkers
Biomarkers - metabolism
Calcitonin
Calcitonin gene-related peptide
Cell Size
Deoxyribonucleic acid
Diseases
DNA
Encephalitis
Ethics
Female
Ganglia
Ganglion cysts
Gene Expression Regulation
Glial cell line-derived neurotrophic factor
Growth factors
Health aspects
Herpes simplex
Herpes simplex virus
Herpes viruses
Herpesvirus 1, Human - metabolism
Herpesvirus 1, Human - physiology
Humans
Immunofluorescence
Infections
Intermediate filament proteins
Latency
Latent infection
Legal medicine
Localization
Male
Middle Aged
Nerve growth factor
Neuritis
Neurological diseases
Neurology
Neurons
Neurons - cytology
Neurons - virology
Nitric oxide
Nitric-oxide synthase
Pain
Paralysis
Peripherin
Populations
Protein Transport
Proteins
Risk factors
Sensory neurons
Signaling
Trigeminal ganglion
Trigeminal Ganglion - cytology
TrkA protein
TrkA receptors
Vestibular system
Viral Proteins - genetics
Viral Proteins - metabolism
Viruses
Young Adult
title Characterization of neuronal populations in the human trigeminal ganglion and their association with latent herpes simplex virus-1 infection
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T11%3A00%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20neuronal%20populations%20in%20the%20human%20trigeminal%20ganglion%20and%20their%20association%20with%20latent%20herpes%20simplex%20virus-1%20infection&rft.jtitle=PloS%20one&rft.au=Flowerdew,%20Sarah%20E&rft.date=2013-12-19&rft.volume=8&rft.issue=12&rft.spage=e83603&rft.epage=e83603&rft.pages=e83603-e83603&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0083603&rft_dat=%3Cgale_plos_%3EA478214990%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1469701698&rft_id=info:pmid/24367603&rft_galeid=A478214990&rft_doaj_id=oai_doaj_org_article_381fa89e65f348aebd7058f8449e0b03&rfr_iscdi=true