Novel microarrays for simultaneous serodiagnosis of multiple antiviral antibodies
We developed an automated diagnostic system for the detection of virus-specific immunoglobulin Gs (IgGs) that was based on a microarray platform. We compared efficacies of our automated system with conventional enzyme immunoassays (EIAs). Viruses were immobilized to microarrays using a radical cross...
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| Veröffentlicht in: | PloS one 2013-12, Vol.8 (12), p.e81726-e81726 |
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| creator | Sivakumar, Ponnurengam Malliappan Moritsugu, Nozomi Obuse, Sei Isoshima, Takashi Tashiro, Hideo Ito, Yoshihiro |
| description | We developed an automated diagnostic system for the detection of virus-specific immunoglobulin Gs (IgGs) that was based on a microarray platform. We compared efficacies of our automated system with conventional enzyme immunoassays (EIAs). Viruses were immobilized to microarrays using a radical cross-linking reaction that was induced by photo-irradiation. A new photoreactive polymer containing perfluorophenyl azide (PFPA) and poly(ethylene glycol) methacrylate was prepared and coated on plates. Inactivated measles, rubella, mumps, Varicella-Zoster and recombinant Epstein-Barr viruse antigen were added to coated plates, and irradiated with ultraviolet light to facilitate immobilization. Virus-specific IgGs in healthy human sera were assayed using these prepared microarrays and the results obtained compared with those from conventional EIAs. We observed high correlation (0.79-0.96) in the results between the automated microarray technique and EIAs. The microarray-based assay was more rapid, involved less reagents and sample, and was easier to conduct compared with conventional EIA techniques. The automated microarray system was further improved by introducing reagent storage reservoirs inside the chamber, thereby conserving the use of expensive reagents and antibodies. We considered the microarray format to be suitable for rapid and multiple serological diagnoses of viral diseases that could be developed further for clinical applications. |
| doi_str_mv | 10.1371/journal.pone.0081726 |
| format | Article |
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We compared efficacies of our automated system with conventional enzyme immunoassays (EIAs). Viruses were immobilized to microarrays using a radical cross-linking reaction that was induced by photo-irradiation. A new photoreactive polymer containing perfluorophenyl azide (PFPA) and poly(ethylene glycol) methacrylate was prepared and coated on plates. Inactivated measles, rubella, mumps, Varicella-Zoster and recombinant Epstein-Barr viruse antigen were added to coated plates, and irradiated with ultraviolet light to facilitate immobilization. Virus-specific IgGs in healthy human sera were assayed using these prepared microarrays and the results obtained compared with those from conventional EIAs. We observed high correlation (0.79-0.96) in the results between the automated microarray technique and EIAs. The microarray-based assay was more rapid, involved less reagents and sample, and was easier to conduct compared with conventional EIA techniques. The automated microarray system was further improved by introducing reagent storage reservoirs inside the chamber, thereby conserving the use of expensive reagents and antibodies. We considered the microarray format to be suitable for rapid and multiple serological diagnoses of viral diseases that could be developed further for clinical applications.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0081726</identifier><identifier>PMID: 24367491</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antibodies ; Antibodies, Viral - blood ; Antigens ; Antiviral agents ; Automation ; Azide ; Azides ; Biochemistry ; Biomedical materials ; Comparative analysis ; Crosslinking ; Diagnostic systems ; Engineering ; Enzymes ; Epstein-Barr virus ; Herpesvirus 4, Human - immunology ; Humans ; Hydrocarbons, Fluorinated ; Immobilization ; Immunoassay ; Immunoassays ; Immunoenzyme Techniques ; Immunoglobulin G - blood ; Immunoglobulins ; Irradiation ; Labeling ; Laboratories ; Measles ; Medical research ; Mumps ; Mumps virus - immunology ; Oligonucleotide Array Sequence Analysis ; Polyethylene glycol ; Polymers ; Reagents ; Rubella ; Rubella virus - immunology ; Serologic Tests - methods ; Sodium ; Storage reservoirs ; Therapeutic applications ; U.V. radiation ; Ultraviolet radiation ; Varicella ; Viral diseases ; Viruses</subject><ispartof>PloS one, 2013-12, Vol.8 (12), p.e81726-e81726</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Sivakumar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Sivakumar et al 2013 Sivakumar et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-bb20d6e8d959f9703bea9eaf5b864df23970536b51226378d454917fb34938043</citedby><cites>FETCH-LOGICAL-c692t-bb20d6e8d959f9703bea9eaf5b864df23970536b51226378d454917fb34938043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867344/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867344/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24367491$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Schildgen, Oliver</contributor><creatorcontrib>Sivakumar, Ponnurengam Malliappan</creatorcontrib><creatorcontrib>Moritsugu, Nozomi</creatorcontrib><creatorcontrib>Obuse, Sei</creatorcontrib><creatorcontrib>Isoshima, Takashi</creatorcontrib><creatorcontrib>Tashiro, Hideo</creatorcontrib><creatorcontrib>Ito, Yoshihiro</creatorcontrib><title>Novel microarrays for simultaneous serodiagnosis of multiple antiviral antibodies</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>We developed an automated diagnostic system for the detection of virus-specific immunoglobulin Gs (IgGs) that was based on a microarray platform. We compared efficacies of our automated system with conventional enzyme immunoassays (EIAs). Viruses were immobilized to microarrays using a radical cross-linking reaction that was induced by photo-irradiation. A new photoreactive polymer containing perfluorophenyl azide (PFPA) and poly(ethylene glycol) methacrylate was prepared and coated on plates. Inactivated measles, rubella, mumps, Varicella-Zoster and recombinant Epstein-Barr viruse antigen were added to coated plates, and irradiated with ultraviolet light to facilitate immobilization. Virus-specific IgGs in healthy human sera were assayed using these prepared microarrays and the results obtained compared with those from conventional EIAs. We observed high correlation (0.79-0.96) in the results between the automated microarray technique and EIAs. The microarray-based assay was more rapid, involved less reagents and sample, and was easier to conduct compared with conventional EIA techniques. The automated microarray system was further improved by introducing reagent storage reservoirs inside the chamber, thereby conserving the use of expensive reagents and antibodies. We considered the microarray format to be suitable for rapid and multiple serological diagnoses of viral diseases that could be developed further for clinical applications.</description><subject>Antibodies</subject><subject>Antibodies, Viral - blood</subject><subject>Antigens</subject><subject>Antiviral agents</subject><subject>Automation</subject><subject>Azide</subject><subject>Azides</subject><subject>Biochemistry</subject><subject>Biomedical materials</subject><subject>Comparative analysis</subject><subject>Crosslinking</subject><subject>Diagnostic systems</subject><subject>Engineering</subject><subject>Enzymes</subject><subject>Epstein-Barr virus</subject><subject>Herpesvirus 4, Human - immunology</subject><subject>Humans</subject><subject>Hydrocarbons, Fluorinated</subject><subject>Immobilization</subject><subject>Immunoassay</subject><subject>Immunoassays</subject><subject>Immunoenzyme Techniques</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulins</subject><subject>Irradiation</subject><subject>Labeling</subject><subject>Laboratories</subject><subject>Measles</subject><subject>Medical research</subject><subject>Mumps</subject><subject>Mumps virus - immunology</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Polyethylene glycol</subject><subject>Polymers</subject><subject>Reagents</subject><subject>Rubella</subject><subject>Rubella virus - immunology</subject><subject>Serologic Tests - methods</subject><subject>Sodium</subject><subject>Storage reservoirs</subject><subject>Therapeutic applications</subject><subject>U.V. radiation</subject><subject>Ultraviolet radiation</subject><subject>Varicella</subject><subject>Viral diseases</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl1v0zAUhiMEYqPwDxBEQkJw0eKv2PEN0jQNqDQx8XlrOclx68qNi51U7N_PWbOpQbtAvrB1_Jzjc16_WfYSowWmAn_Y-D602i12voUFQiUWhD_KTrGkZM4Joo-PzifZsxg3CBW05PxpdkIY5YJJfJp9--r34PKtrYPXIejrmBsf8mi3vet0C76PeYTgG6tXrY825t7kw53dOch129m9DdrdnqpEQXyePTHaRXgx7rPs16eLn-df5pdXn5fnZ5fzmkvSzauKoIZD2chCGikQrUBL0KaoSs4aQ2iKFZRXBSaEU1E2rEgNC1NRJmmJGJ1lrw91d85HNaoRFWZcUsQKLhKxPBCN1xu1C3arw7Xy2qrbgA8rpUNnawfKNKbgnEMtCDBKhU5NIWyM0JjXLCk4yz6Or_XVFpoa2i6NPSk6vWntWq38XiXFBWVDu-_GAsH_6SF2amtjDc4dRE59SySIZLhM6Jt_0IenG6mVTgPY1vj0bj0UVWdMlIQMoiZq8QCVVgPpz5N1jE3xScL7SUJiOvjbrXQfo1r--P7_7NXvKfv2iF2Ddt06etd31rdxCrIDmBwZYwBzLzJGanD-nRpqcL4anZ_SXh1_0H3SndXpDdgo_gk</recordid><startdate>20131218</startdate><enddate>20131218</enddate><creator>Sivakumar, Ponnurengam Malliappan</creator><creator>Moritsugu, Nozomi</creator><creator>Obuse, Sei</creator><creator>Isoshima, Takashi</creator><creator>Tashiro, Hideo</creator><creator>Ito, Yoshihiro</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131218</creationdate><title>Novel microarrays for simultaneous serodiagnosis of multiple antiviral antibodies</title><author>Sivakumar, Ponnurengam Malliappan ; Moritsugu, Nozomi ; Obuse, Sei ; Isoshima, Takashi ; Tashiro, Hideo ; Ito, Yoshihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-bb20d6e8d959f9703bea9eaf5b864df23970536b51226378d454917fb34938043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antibodies</topic><topic>Antibodies, Viral - blood</topic><topic>Antigens</topic><topic>Antiviral agents</topic><topic>Automation</topic><topic>Azide</topic><topic>Azides</topic><topic>Biochemistry</topic><topic>Biomedical materials</topic><topic>Comparative analysis</topic><topic>Crosslinking</topic><topic>Diagnostic systems</topic><topic>Engineering</topic><topic>Enzymes</topic><topic>Epstein-Barr virus</topic><topic>Herpesvirus 4, Human - immunology</topic><topic>Humans</topic><topic>Hydrocarbons, Fluorinated</topic><topic>Immobilization</topic><topic>Immunoassay</topic><topic>Immunoassays</topic><topic>Immunoenzyme Techniques</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulins</topic><topic>Irradiation</topic><topic>Labeling</topic><topic>Laboratories</topic><topic>Measles</topic><topic>Medical research</topic><topic>Mumps</topic><topic>Mumps virus - immunology</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Polyethylene glycol</topic><topic>Polymers</topic><topic>Reagents</topic><topic>Rubella</topic><topic>Rubella virus - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sivakumar, Ponnurengam Malliappan</au><au>Moritsugu, Nozomi</au><au>Obuse, Sei</au><au>Isoshima, Takashi</au><au>Tashiro, Hideo</au><au>Ito, Yoshihiro</au><au>Schildgen, Oliver</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel microarrays for simultaneous serodiagnosis of multiple antiviral antibodies</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-12-18</date><risdate>2013</risdate><volume>8</volume><issue>12</issue><spage>e81726</spage><epage>e81726</epage><pages>e81726-e81726</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>We developed an automated diagnostic system for the detection of virus-specific immunoglobulin Gs (IgGs) that was based on a microarray platform. We compared efficacies of our automated system with conventional enzyme immunoassays (EIAs). Viruses were immobilized to microarrays using a radical cross-linking reaction that was induced by photo-irradiation. A new photoreactive polymer containing perfluorophenyl azide (PFPA) and poly(ethylene glycol) methacrylate was prepared and coated on plates. Inactivated measles, rubella, mumps, Varicella-Zoster and recombinant Epstein-Barr viruse antigen were added to coated plates, and irradiated with ultraviolet light to facilitate immobilization. Virus-specific IgGs in healthy human sera were assayed using these prepared microarrays and the results obtained compared with those from conventional EIAs. We observed high correlation (0.79-0.96) in the results between the automated microarray technique and EIAs. The microarray-based assay was more rapid, involved less reagents and sample, and was easier to conduct compared with conventional EIA techniques. The automated microarray system was further improved by introducing reagent storage reservoirs inside the chamber, thereby conserving the use of expensive reagents and antibodies. We considered the microarray format to be suitable for rapid and multiple serological diagnoses of viral diseases that could be developed further for clinical applications.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24367491</pmid><doi>10.1371/journal.pone.0081726</doi><tpages>e81726</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies Antibodies, Viral - blood Antigens Antiviral agents Automation Azide Azides Biochemistry Biomedical materials Comparative analysis Crosslinking Diagnostic systems Engineering Enzymes Epstein-Barr virus Herpesvirus 4, Human - immunology Humans Hydrocarbons, Fluorinated Immobilization Immunoassay Immunoassays Immunoenzyme Techniques Immunoglobulin G - blood Immunoglobulins Irradiation Labeling Laboratories Measles Medical research Mumps Mumps virus - immunology Oligonucleotide Array Sequence Analysis Polyethylene glycol Polymers Reagents Rubella Rubella virus - immunology Serologic Tests - methods Sodium Storage reservoirs Therapeutic applications U.V. radiation Ultraviolet radiation Varicella Viral diseases Viruses |
| title | Novel microarrays for simultaneous serodiagnosis of multiple antiviral antibodies |
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