Genetic variability and evolutionary implications of RNA silencing suppressor genes in RNA1 of sweet potato chlorotic stunt virus isolates infecting sweetpotato and related wild species
The bipartite single-stranded RNA genome of Sweet potato chlorotic stunt virus (SPCSV, genus Crinivirus; Closteroviridae) encodes a Class 1 RNase III (RNase3), a putative hydrophobic protein (p7) and a 22-kDa protein (p22) from genes located in RNA1. RNase3 and p22 suppress RNA silencing, the basal...
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| description | The bipartite single-stranded RNA genome of Sweet potato chlorotic stunt virus (SPCSV, genus Crinivirus; Closteroviridae) encodes a Class 1 RNase III (RNase3), a putative hydrophobic protein (p7) and a 22-kDa protein (p22) from genes located in RNA1. RNase3 and p22 suppress RNA silencing, the basal antiviral defence mechanism in plants. RNase3 is sufficient to render sweetpotato (Ipomoea batatas) virus-susceptible and predisposes it to development of severe diseases following infection with unrelated virus. The incidence, strains and gene content of SPCSV infecting wild plant species have not been studied.
Thirty SPCSV isolates were characterized from 10 wild Ipomoea species, Hewittia sublobata or Lepistemon owariensis (family Convolvulaceae) in Uganda and compared with 34 local SPCSV isolates infecting sweetpotatoes. All isolates belonged to the East African (EA) strain of SPCSV and contained RNase3 and p7, but p22 was not detected in six isolates. The three genes showed only limited genetic variability and the proteins were under purifying selection. SPCSV isolates lacking p22 synergized with Sweet potato feathery mottle virus (SPFMV, genus potyvirus; Potyviridae) and caused severe symptoms in co-infected sweetpotato plants. One SPCSV isolate enhanced accumulation of SPFMV, but no severe symptoms developed. A new whitefly-transmitted virus (KML33b) encoding an RNase3 homolog ( |
| doi_str_mv | 10.1371/journal.pone.0081479 |
| format | Article |
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Thirty SPCSV isolates were characterized from 10 wild Ipomoea species, Hewittia sublobata or Lepistemon owariensis (family Convolvulaceae) in Uganda and compared with 34 local SPCSV isolates infecting sweetpotatoes. All isolates belonged to the East African (EA) strain of SPCSV and contained RNase3 and p7, but p22 was not detected in six isolates. The three genes showed only limited genetic variability and the proteins were under purifying selection. SPCSV isolates lacking p22 synergized with Sweet potato feathery mottle virus (SPFMV, genus potyvirus; Potyviridae) and caused severe symptoms in co-infected sweetpotato plants. One SPCSV isolate enhanced accumulation of SPFMV, but no severe symptoms developed. A new whitefly-transmitted virus (KML33b) encoding an RNase3 homolog (<56% identity to SPCSV RNase3) able to suppresses sense-mediated RNA silencing was detected in I. sinensis.
SPCSV isolates infecting wild species and sweetpotato in Uganda were genetically undifferentiated, suggesting inter-species transmission of SPCSV. Most isolates in Uganda contained p22, unlike SPCSV isolates characterized from other countries and continents. Enhanced accumulation of SPFMV and increased disease severity were found to be uncoupled phenotypic outcomes of RNase3-mediated viral synergism in sweetpotato. A second virus encoding an RNase3-like RNA silencing suppressor was detected. Overall, results provided many novel and important insights into evolutionary biology of SPCSV.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0081479</identifier><identifier>PMID: 24278443</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accumulation ; Agriculture ; Amino Acid Sequence ; Bioinformatics ; Biological effects ; Biological evolution ; Closterovirus ; Continents ; Crinivirus - genetics ; Development and progression ; Environmental science ; Evolution & development ; Evolution, Molecular ; Gene silencing ; Genes ; Genes, Suppressor ; Genetic engineering ; Genetic research ; Genetic variability ; Genetic Variation ; Genomes ; Genomics ; Health aspects ; Homology ; Hydrolases ; Hydrophobicity ; Infection ; Ipomoea ; Ipomoea batatas ; Ipomoea batatas - classification ; Ipomoea batatas - virology ; Molecular Sequence Data ; Phenotype ; Phylogenetics ; Phylogeny ; Plant Diseases - virology ; Plant species ; Plant virus diseases ; Plant viruses ; Plants (botany) ; Plants (Organisms) ; Potatoes ; Potyvirus ; Proteins ; Ribonuclease ; Ribonuclease III ; Ribonucleic acid ; RNA ; RNA viruses ; RNA, Viral - genetics ; RNA-mediated interference ; Selection, Genetic ; Sequence Alignment ; Serotyping ; Sweet potatoes ; Synergism ; Taste (Sense) ; Variability ; Viral Proteins - genetics ; Viruses</subject><ispartof>PloS one, 2013-11, Vol.8 (11), p.e81479-e81479</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Tugume et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/3.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Tugume et al 2013 Tugume et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-b49ee0ca7b34a5923ac5be081a5196329be624164d88eee32456d493d11953423</citedby><cites>FETCH-LOGICAL-c692t-b49ee0ca7b34a5923ac5be081a5196329be624164d88eee32456d493d11953423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838340/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838340/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24278443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Rao, A.L.N.</contributor><creatorcontrib>Tugume, Arthur K</creatorcontrib><creatorcontrib>Amayo, Robert</creatorcontrib><creatorcontrib>Weinheimer, Isabel</creatorcontrib><creatorcontrib>Mukasa, Settumba B</creatorcontrib><creatorcontrib>Rubaihayo, Patrick R</creatorcontrib><creatorcontrib>Valkonen, Jari P T</creatorcontrib><title>Genetic variability and evolutionary implications of RNA silencing suppressor genes in RNA1 of sweet potato chlorotic stunt virus isolates infecting sweetpotato and related wild species</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The bipartite single-stranded RNA genome of Sweet potato chlorotic stunt virus (SPCSV, genus Crinivirus; Closteroviridae) encodes a Class 1 RNase III (RNase3), a putative hydrophobic protein (p7) and a 22-kDa protein (p22) from genes located in RNA1. RNase3 and p22 suppress RNA silencing, the basal antiviral defence mechanism in plants. RNase3 is sufficient to render sweetpotato (Ipomoea batatas) virus-susceptible and predisposes it to development of severe diseases following infection with unrelated virus. The incidence, strains and gene content of SPCSV infecting wild plant species have not been studied.
Thirty SPCSV isolates were characterized from 10 wild Ipomoea species, Hewittia sublobata or Lepistemon owariensis (family Convolvulaceae) in Uganda and compared with 34 local SPCSV isolates infecting sweetpotatoes. All isolates belonged to the East African (EA) strain of SPCSV and contained RNase3 and p7, but p22 was not detected in six isolates. The three genes showed only limited genetic variability and the proteins were under purifying selection. SPCSV isolates lacking p22 synergized with Sweet potato feathery mottle virus (SPFMV, genus potyvirus; Potyviridae) and caused severe symptoms in co-infected sweetpotato plants. One SPCSV isolate enhanced accumulation of SPFMV, but no severe symptoms developed. A new whitefly-transmitted virus (KML33b) encoding an RNase3 homolog (<56% identity to SPCSV RNase3) able to suppresses sense-mediated RNA silencing was detected in I. sinensis.
SPCSV isolates infecting wild species and sweetpotato in Uganda were genetically undifferentiated, suggesting inter-species transmission of SPCSV. Most isolates in Uganda contained p22, unlike SPCSV isolates characterized from other countries and continents. Enhanced accumulation of SPFMV and increased disease severity were found to be uncoupled phenotypic outcomes of RNase3-mediated viral synergism in sweetpotato. A second virus encoding an RNase3-like RNA silencing suppressor was detected. Overall, results provided many novel and important insights into evolutionary biology of SPCSV.</description><subject>Accumulation</subject><subject>Agriculture</subject><subject>Amino Acid Sequence</subject><subject>Bioinformatics</subject><subject>Biological effects</subject><subject>Biological evolution</subject><subject>Closterovirus</subject><subject>Continents</subject><subject>Crinivirus - genetics</subject><subject>Development and progression</subject><subject>Environmental science</subject><subject>Evolution & development</subject><subject>Evolution, Molecular</subject><subject>Gene silencing</subject><subject>Genes</subject><subject>Genes, Suppressor</subject><subject>Genetic engineering</subject><subject>Genetic research</subject><subject>Genetic variability</subject><subject>Genetic Variation</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Health aspects</subject><subject>Homology</subject><subject>Hydrolases</subject><subject>Hydrophobicity</subject><subject>Infection</subject><subject>Ipomoea</subject><subject>Ipomoea batatas</subject><subject>Ipomoea batatas - classification</subject><subject>Ipomoea batatas - virology</subject><subject>Molecular Sequence Data</subject><subject>Phenotype</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant Diseases - virology</subject><subject>Plant species</subject><subject>Plant virus diseases</subject><subject>Plant viruses</subject><subject>Plants (botany)</subject><subject>Plants (Organisms)</subject><subject>Potatoes</subject><subject>Potyvirus</subject><subject>Proteins</subject><subject>Ribonuclease</subject><subject>Ribonuclease III</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA viruses</subject><subject>RNA, Viral - genetics</subject><subject>RNA-mediated interference</subject><subject>Selection, Genetic</subject><subject>Sequence Alignment</subject><subject>Serotyping</subject><subject>Sweet potatoes</subject><subject>Synergism</subject><subject>Taste (Sense)</subject><subject>Variability</subject><subject>Viral Proteins - genetics</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99u0zAUxiMEYmPwBggsISG4aLFjx4lvkKoJRqWJSePPreU4p60rNw6207FH4-1w2mxq0S6QLxInv-87PsfnZNlLgqeEluTD2vW-VXbauRamGFeEleJRdkoEzSc8x_TxwftJ9iyENcYFrTh_mp3kLC8rxuhp9ucCWohGo63yRtXGmniLVNsg2DrbR-Na5W-R2XTWaDVsA3ILdP11hoKx0GrTLlHou85DCM6jZXILyLQDQQYy3ABE1LmookN6ZZ13Q7QQ-zairfF9ooOzKu5kC9Bx5zioRtFwGA8D0aAbYxsUOtAGwvPsyULZAC_G51n24_On7-dfJpdXF_Pz2eVEc5HHSc0EANaqrClThcip0kUNqVyqIILTXNTAc0Y4a6oKAGjOCt4wQRtCREFZTs-y13vfzrogx6oHSRivBBUlYYmY74nGqbXsvNmkmkmnjNx9cH4plU9ZW5CYC6iFKjVThBGGFQYhmK6g4bzhIJLXxzFaX2-g0dBGr-yR6fGf1qzk0m0lrdJiOBm8Gw28-9VDiHJjggZrVQuu3507JwKTasjszT_ow9mN1FKlBNIduRRXD6ZyxsqK8CLfeU0foNJqYGN0atFFapdjwfsjQWIi_I5L1Ycg59-u_5-9-nnMvj1gV6BsXIWxlcMxyPag9i4ED4v7IhMshwm7q4YcJkyOE5Zkrw4v6F50N1L0L5i7JNc</recordid><startdate>20131122</startdate><enddate>20131122</enddate><creator>Tugume, Arthur K</creator><creator>Amayo, Robert</creator><creator>Weinheimer, Isabel</creator><creator>Mukasa, Settumba B</creator><creator>Rubaihayo, Patrick R</creator><creator>Valkonen, Jari P T</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131122</creationdate><title>Genetic variability and evolutionary implications of RNA silencing suppressor genes in RNA1 of sweet potato chlorotic stunt virus isolates infecting sweetpotato and related wild species</title><author>Tugume, Arthur K ; Amayo, Robert ; Weinheimer, Isabel ; Mukasa, Settumba B ; Rubaihayo, Patrick R ; Valkonen, Jari P T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-b49ee0ca7b34a5923ac5be081a5196329be624164d88eee32456d493d11953423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Accumulation</topic><topic>Agriculture</topic><topic>Amino Acid Sequence</topic><topic>Bioinformatics</topic><topic>Biological effects</topic><topic>Biological evolution</topic><topic>Closterovirus</topic><topic>Continents</topic><topic>Crinivirus - genetics</topic><topic>Development and progression</topic><topic>Environmental science</topic><topic>Evolution & development</topic><topic>Evolution, Molecular</topic><topic>Gene silencing</topic><topic>Genes</topic><topic>Genes, Suppressor</topic><topic>Genetic engineering</topic><topic>Genetic research</topic><topic>Genetic variability</topic><topic>Genetic Variation</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Health aspects</topic><topic>Homology</topic><topic>Hydrolases</topic><topic>Hydrophobicity</topic><topic>Infection</topic><topic>Ipomoea</topic><topic>Ipomoea batatas</topic><topic>Ipomoea batatas - classification</topic><topic>Ipomoea batatas - virology</topic><topic>Molecular Sequence Data</topic><topic>Phenotype</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Plant Diseases - virology</topic><topic>Plant species</topic><topic>Plant virus diseases</topic><topic>Plant viruses</topic><topic>Plants (botany)</topic><topic>Plants (Organisms)</topic><topic>Potatoes</topic><topic>Potyvirus</topic><topic>Proteins</topic><topic>Ribonuclease</topic><topic>Ribonuclease III</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA viruses</topic><topic>RNA, Viral - genetics</topic><topic>RNA-mediated interference</topic><topic>Selection, Genetic</topic><topic>Sequence Alignment</topic><topic>Serotyping</topic><topic>Sweet potatoes</topic><topic>Synergism</topic><topic>Taste (Sense)</topic><topic>Variability</topic><topic>Viral Proteins - genetics</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tugume, Arthur K</creatorcontrib><creatorcontrib>Amayo, Robert</creatorcontrib><creatorcontrib>Weinheimer, Isabel</creatorcontrib><creatorcontrib>Mukasa, Settumba B</creatorcontrib><creatorcontrib>Rubaihayo, Patrick R</creatorcontrib><creatorcontrib>Valkonen, Jari P T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tugume, Arthur K</au><au>Amayo, Robert</au><au>Weinheimer, Isabel</au><au>Mukasa, Settumba B</au><au>Rubaihayo, Patrick R</au><au>Valkonen, Jari P T</au><au>Rao, A.L.N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic variability and evolutionary implications of RNA silencing suppressor genes in RNA1 of sweet potato chlorotic stunt virus isolates infecting sweetpotato and related wild species</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-11-22</date><risdate>2013</risdate><volume>8</volume><issue>11</issue><spage>e81479</spage><epage>e81479</epage><pages>e81479-e81479</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The bipartite single-stranded RNA genome of Sweet potato chlorotic stunt virus (SPCSV, genus Crinivirus; Closteroviridae) encodes a Class 1 RNase III (RNase3), a putative hydrophobic protein (p7) and a 22-kDa protein (p22) from genes located in RNA1. RNase3 and p22 suppress RNA silencing, the basal antiviral defence mechanism in plants. RNase3 is sufficient to render sweetpotato (Ipomoea batatas) virus-susceptible and predisposes it to development of severe diseases following infection with unrelated virus. The incidence, strains and gene content of SPCSV infecting wild plant species have not been studied.
Thirty SPCSV isolates were characterized from 10 wild Ipomoea species, Hewittia sublobata or Lepistemon owariensis (family Convolvulaceae) in Uganda and compared with 34 local SPCSV isolates infecting sweetpotatoes. All isolates belonged to the East African (EA) strain of SPCSV and contained RNase3 and p7, but p22 was not detected in six isolates. The three genes showed only limited genetic variability and the proteins were under purifying selection. SPCSV isolates lacking p22 synergized with Sweet potato feathery mottle virus (SPFMV, genus potyvirus; Potyviridae) and caused severe symptoms in co-infected sweetpotato plants. One SPCSV isolate enhanced accumulation of SPFMV, but no severe symptoms developed. A new whitefly-transmitted virus (KML33b) encoding an RNase3 homolog (<56% identity to SPCSV RNase3) able to suppresses sense-mediated RNA silencing was detected in I. sinensis.
SPCSV isolates infecting wild species and sweetpotato in Uganda were genetically undifferentiated, suggesting inter-species transmission of SPCSV. Most isolates in Uganda contained p22, unlike SPCSV isolates characterized from other countries and continents. Enhanced accumulation of SPFMV and increased disease severity were found to be uncoupled phenotypic outcomes of RNase3-mediated viral synergism in sweetpotato. A second virus encoding an RNase3-like RNA silencing suppressor was detected. Overall, results provided many novel and important insights into evolutionary biology of SPCSV.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24278443</pmid><doi>10.1371/journal.pone.0081479</doi><tpages>e81479</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-11, Vol.8 (11), p.e81479-e81479 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1468939714 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Accumulation Agriculture Amino Acid Sequence Bioinformatics Biological effects Biological evolution Closterovirus Continents Crinivirus - genetics Development and progression Environmental science Evolution & development Evolution, Molecular Gene silencing Genes Genes, Suppressor Genetic engineering Genetic research Genetic variability Genetic Variation Genomes Genomics Health aspects Homology Hydrolases Hydrophobicity Infection Ipomoea Ipomoea batatas Ipomoea batatas - classification Ipomoea batatas - virology Molecular Sequence Data Phenotype Phylogenetics Phylogeny Plant Diseases - virology Plant species Plant virus diseases Plant viruses Plants (botany) Plants (Organisms) Potatoes Potyvirus Proteins Ribonuclease Ribonuclease III Ribonucleic acid RNA RNA viruses RNA, Viral - genetics RNA-mediated interference Selection, Genetic Sequence Alignment Serotyping Sweet potatoes Synergism Taste (Sense) Variability Viral Proteins - genetics Viruses |
| title | Genetic variability and evolutionary implications of RNA silencing suppressor genes in RNA1 of sweet potato chlorotic stunt virus isolates infecting sweetpotato and related wild species |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T10%3A21%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20variability%20and%20evolutionary%20implications%20of%20RNA%20silencing%20suppressor%20genes%20in%20RNA1%20of%20sweet%20potato%20chlorotic%20stunt%20virus%20isolates%20infecting%20sweetpotato%20and%20related%20wild%20species&rft.jtitle=PloS%20one&rft.au=Tugume,%20Arthur%20K&rft.date=2013-11-22&rft.volume=8&rft.issue=11&rft.spage=e81479&rft.epage=e81479&rft.pages=e81479-e81479&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0081479&rft_dat=%3Cgale_plos_%3EA478165282%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1468939714&rft_id=info:pmid/24278443&rft_galeid=A478165282&rft_doaj_id=oai_doaj_org_article_069eb9a7c4a14140a0e994c8ed66d6e9&rfr_iscdi=true |