Selection and validation of reference genes for real-time quantitative PCR in hyperaccumulating ecotype of Sedum alfredii under different heavy metals stresses

Real-time Quantitative PCR (RT-qPCR) has become an effective method for accurate analysis of gene expression in several biological systems as well as under different experimental conditions. Although with high sensitivity, specificity and broad dynamic range, this method requires suitable reference...

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Veröffentlicht in:	PloS one 2013-12, Vol.8 (12), p.e82927
Hauptverfasser:	Sang, Jian, Han, Xiaojiao, Liu, Mingying, Qiao, Guirong, Jiang, Jing, Zhuo, Renying
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Analysis Base Sequence Bemisia tabaci Biodegradation, Environmental Biological effects Bioremediation Cadmium Cadmium - chemistry Copper Copper - chemistry DNA Primers - genetics Ecotype Efficiency Gene expression Gene Expression Profiling - methods Gene Expression Regulation, Plant Genes Genes, Essential Genes, Plant Genomics Heavy metals Laboratories Lead Lead - chemistry Metals, Heavy - chemistry Molecular Sequence Data Phytoremediation Plant Proteins - genetics Plants (botany) Polymerase chain reaction Proteins Real time Real-Time Polymerase Chain Reaction - methods Reference Standards Reproducibility of Results Sedum - drug effects Sedum - genetics Sedum alfredii Stresses Studies System effectiveness Transcription Trees Zinc Zinc - chemistry
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