Highly efficient generation of GGTA1 biallelic knockout inbred mini-pigs with TALENs

Inbred mini-pigs are ideal organ donors for future human xenotransplantations because of their clear genetic background, high homozygosity, and high inbreeding endurance. In this study, we chose fibroblast cells from a highly inbred pig line called Banna mini-pig inbred line (BMI) as donor nuclei fo...

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Veröffentlicht in:	PloS one 2013-12, Vol.8 (12), p.e84250-e84250
Hauptverfasser:	Xin, Jige, Yang, Huaqiang, Fan, Nana, Zhao, Bentian, Ouyang, Zhen, Liu, Zhaoming, Zhao, Yu, Li, Xiaoping, Song, Jun, Yang, Yi, Zou, Qingjian, Yan, Quanmei, Zeng, Yangzhi, Lai, Liangxue
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Animal sciences Animals Animals, Genetically Modified Base Sequence Biology Blastocysts Body mass Cell lines Chief executive officers Cloning Colonies Deoxyribonucleic acid DNA DNA binding proteins Efficiency Embryonic development Embryos Endurance Epitopes Fetuses Fibroblasts Fibroblasts - metabolism Galactosyltransferases - chemistry Galactosyltransferases - genetics Gene Knockout Techniques Gene Targeting Genetic engineering Genomes GGTA1 gene Hogs Homozygosity Inbreeding Laboratories Lysis Medicine Mutation Neomycin Nuclear transfer Nuclease Nucleases Nuclei Organ donors Organ transplantation Phenotype Rodents Somatic cell nuclear transfer Stem cells Suidae Swine Swine, Miniature - genetics Transcription Transcription activator-like effector nucleases Xenografts Xenopus Xenotransplantation Zoology
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