Live birth from slow-frozen rabbit oocytes after in vivo fertilisation
In vivo fertilisation techniques such as intraoviductal oocyte transfer have been considered as alternatives to bypass the inadequacy of conventional in vitro fertilisation in rabbit. There is only one study in the literature, published in 1989, that reports live offspring from cryopreserved rabbit...
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| description | In vivo fertilisation techniques such as intraoviductal oocyte transfer have been considered as alternatives to bypass the inadequacy of conventional in vitro fertilisation in rabbit. There is only one study in the literature, published in 1989, that reports live offspring from cryopreserved rabbit oocytes. The aim of the present study was to establish the in vivo fertilisation procedure to generate live offspring with frozen oocytes. First, the effect of two recipient models (i) ovariectomised or (ii) oviduct ligated immediately after transfer on the ability of fresh oocytes to fertilise were compared. Second, generation of live offspring from slow-frozen oocytes was carried out using the ligated oviduct recipient model. Throughout the experiment, recipients were artificially inseminated 9 hours prior to oocyte transfer. In the first experiment, two days after unilateral transfer of fresh oocytes, oviducts and uterine horns were flushed to assess embryo recovery rates. The embryo recovery rates were low compared to control in both ovariectomised and ligated oviduct groups. However, ligated oviduct recipient showed significantly (P |
| doi_str_mv | 10.1371/journal.pone.0083399 |
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There is only one study in the literature, published in 1989, that reports live offspring from cryopreserved rabbit oocytes. The aim of the present study was to establish the in vivo fertilisation procedure to generate live offspring with frozen oocytes. First, the effect of two recipient models (i) ovariectomised or (ii) oviduct ligated immediately after transfer on the ability of fresh oocytes to fertilise were compared. Second, generation of live offspring from slow-frozen oocytes was carried out using the ligated oviduct recipient model. Throughout the experiment, recipients were artificially inseminated 9 hours prior to oocyte transfer. In the first experiment, two days after unilateral transfer of fresh oocytes, oviducts and uterine horns were flushed to assess embryo recovery rates. The embryo recovery rates were low compared to control in both ovariectomised and ligated oviduct groups. However, ligated oviduct recipient showed significantly (P<0.05) higher embryo recovery rates compared to ovariectomised and control-transferred. In the second experiment, using bilateral oviduct ligation model, all females that received slow-frozen oocytes became pregnant and delivered a total of 4 live young naturally. Thus, in vivo fertilisation is an effective technique to generate live offspring using slow-frozen oocytes in rabbits.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0083399</identifier><identifier>PMID: 24358281</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animal models ; Animals ; Cryopreservation ; Cryopreservation - methods ; Embryo Transfer - veterinary ; Embryonic development ; Embryos ; Female ; Females ; Fertilization ; Fertilization in Vitro - methods ; Fertilization in Vitro - veterinary ; Freezing ; In vitro fertilization ; In vivo methods and tests ; Laparoscopy ; Live Birth - veterinary ; Offspring ; Oocyte Retrieval - veterinary ; Oocytes ; Ovariectomy - veterinary ; Oviduct ; Pregnancy ; Rabbits ; Recovery ; Reproducibility of Results ; Sperm ; Sterilization, Tubal - veterinary ; Uterus</subject><ispartof>PloS one, 2013-12, Vol.8 (12), p.e83399-e83399</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Jiménez-Trigos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Jiménez-Trigos et al 2013 Jiménez-Trigos et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-9ae6b604b3adf29bed6cb378198f4fc4e8e76014deb123532adb7305bc3c42863</citedby><cites>FETCH-LOGICAL-c692t-9ae6b604b3adf29bed6cb378198f4fc4e8e76014deb123532adb7305bc3c42863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3866232/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3866232/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24358281$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sun, Qing-Yuan</contributor><creatorcontrib>Jiménez-Trigos, Estrella</creatorcontrib><creatorcontrib>Vicente, José S</creatorcontrib><creatorcontrib>Marco-Jiménez, Francisco</creatorcontrib><title>Live birth from slow-frozen rabbit oocytes after in vivo fertilisation</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In vivo fertilisation techniques such as intraoviductal oocyte transfer have been considered as alternatives to bypass the inadequacy of conventional in vitro fertilisation in rabbit. There is only one study in the literature, published in 1989, that reports live offspring from cryopreserved rabbit oocytes. The aim of the present study was to establish the in vivo fertilisation procedure to generate live offspring with frozen oocytes. First, the effect of two recipient models (i) ovariectomised or (ii) oviduct ligated immediately after transfer on the ability of fresh oocytes to fertilise were compared. Second, generation of live offspring from slow-frozen oocytes was carried out using the ligated oviduct recipient model. Throughout the experiment, recipients were artificially inseminated 9 hours prior to oocyte transfer. In the first experiment, two days after unilateral transfer of fresh oocytes, oviducts and uterine horns were flushed to assess embryo recovery rates. The embryo recovery rates were low compared to control in both ovariectomised and ligated oviduct groups. However, ligated oviduct recipient showed significantly (P<0.05) higher embryo recovery rates compared to ovariectomised and control-transferred. In the second experiment, using bilateral oviduct ligation model, all females that received slow-frozen oocytes became pregnant and delivered a total of 4 live young naturally. Thus, in vivo fertilisation is an effective technique to generate live offspring using slow-frozen oocytes in rabbits.</description><subject>Analysis</subject><subject>Animal models</subject><subject>Animals</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Embryo Transfer - veterinary</subject><subject>Embryonic development</subject><subject>Embryos</subject><subject>Female</subject><subject>Females</subject><subject>Fertilization</subject><subject>Fertilization in Vitro - methods</subject><subject>Fertilization in Vitro - veterinary</subject><subject>Freezing</subject><subject>In vitro fertilization</subject><subject>In vivo methods and tests</subject><subject>Laparoscopy</subject><subject>Live Birth - veterinary</subject><subject>Offspring</subject><subject>Oocyte Retrieval - veterinary</subject><subject>Oocytes</subject><subject>Ovariectomy - veterinary</subject><subject>Oviduct</subject><subject>Pregnancy</subject><subject>Rabbits</subject><subject>Recovery</subject><subject>Reproducibility of Results</subject><subject>Sperm</subject><subject>Sterilization, Tubal - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiménez-Trigos, Estrella</au><au>Vicente, José S</au><au>Marco-Jiménez, Francisco</au><au>Sun, Qing-Yuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Live birth from slow-frozen rabbit oocytes after in vivo fertilisation</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-12-17</date><risdate>2013</risdate><volume>8</volume><issue>12</issue><spage>e83399</spage><epage>e83399</epage><pages>e83399-e83399</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In vivo fertilisation techniques such as intraoviductal oocyte transfer have been considered as alternatives to bypass the inadequacy of conventional in vitro fertilisation in rabbit. There is only one study in the literature, published in 1989, that reports live offspring from cryopreserved rabbit oocytes. The aim of the present study was to establish the in vivo fertilisation procedure to generate live offspring with frozen oocytes. First, the effect of two recipient models (i) ovariectomised or (ii) oviduct ligated immediately after transfer on the ability of fresh oocytes to fertilise were compared. Second, generation of live offspring from slow-frozen oocytes was carried out using the ligated oviduct recipient model. Throughout the experiment, recipients were artificially inseminated 9 hours prior to oocyte transfer. In the first experiment, two days after unilateral transfer of fresh oocytes, oviducts and uterine horns were flushed to assess embryo recovery rates. The embryo recovery rates were low compared to control in both ovariectomised and ligated oviduct groups. However, ligated oviduct recipient showed significantly (P<0.05) higher embryo recovery rates compared to ovariectomised and control-transferred. In the second experiment, using bilateral oviduct ligation model, all females that received slow-frozen oocytes became pregnant and delivered a total of 4 live young naturally. Thus, in vivo fertilisation is an effective technique to generate live offspring using slow-frozen oocytes in rabbits.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24358281</pmid><doi>10.1371/journal.pone.0083399</doi><tpages>e83399</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animal models Animals Cryopreservation Cryopreservation - methods Embryo Transfer - veterinary Embryonic development Embryos Female Females Fertilization Fertilization in Vitro - methods Fertilization in Vitro - veterinary Freezing In vitro fertilization In vivo methods and tests Laparoscopy Live Birth - veterinary Offspring Oocyte Retrieval - veterinary Oocytes Ovariectomy - veterinary Oviduct Pregnancy Rabbits Recovery Reproducibility of Results Sperm Sterilization, Tubal - veterinary Uterus |
| title | Live birth from slow-frozen rabbit oocytes after in vivo fertilisation |
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