Distribution of endophytic bacteria in Alopecurus aequalis Sobol and Oxalis corniculata L. from soils contaminated by polycyclic aromatic hydrocarbons
The distributions of endophytic bacteria in Alopecurus aequalis Sobol and Oxalis corniculata L. grown in soils contaminated with different levels of polycyclic aromatic hydrocarbons (PAHs) were investigated with polymerase chain reaction followed by denaturing gradient gel electrophoresis technology...
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| description | The distributions of endophytic bacteria in Alopecurus aequalis Sobol and Oxalis corniculata L. grown in soils contaminated with different levels of polycyclic aromatic hydrocarbons (PAHs) were investigated with polymerase chain reaction followed by denaturing gradient gel electrophoresis technology (PCR-DGGE) and cultivation methods. Twelve types of PAHs, at concentrations varying from 0.16 to 180 mg·kg(-1), were observed in the roots and shoots of the two plants. The total PAH concentrations in Alopecurus aequalis Sobol obtained from three different PAH-contaminated stations were 184, 197, and 304 mg·kg(-1), and the total PAH concentrations in Oxalis corniculata L. were 251, 346, and 600 mg·kg(-1), respectively. The PCR-DGGE results showed that the endophytic bacterial communities in the roots and shoots of the two plants were quite different, although most bacteria belonged to Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes. A total of 68 endophytic bacterial strains were isolated from different tissues of the two plants and classified into three phyla: Firmicutes, Proteobacteria and Bacteroidetes. In both plants, Bacillus spp. and Pseudomonas spp. were the dominant cultivable populations. With an increase in the PAH pollution level, the diversity and distribution of endophytic bacteria in the two plants changed correspondingly, and the number of cultivable endophytic bacterial strains decreased rapidly. Testing of the isolated endophytic bacteria for tolerance to each type of PAH showed that most isolates could grow well on Luria-Bertani media in the presence of different PAHs, and some isolates were able to grow rapidly on a mineral salt medium with a single PAH as the sole carbon and energy source, indicating that these strains may have the potential to degrade PAHs in plants. This research provides the first insight into the characteristics of endophytic bacterial populations under different PAH pollution levels and provides a species resource for the isolation of PAH-degrading endophytic bacteria. |
| doi_str_mv | 10.1371/journal.pone.0083054 |
| format | Article |
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Twelve types of PAHs, at concentrations varying from 0.16 to 180 mg·kg(-1), were observed in the roots and shoots of the two plants. The total PAH concentrations in Alopecurus aequalis Sobol obtained from three different PAH-contaminated stations were 184, 197, and 304 mg·kg(-1), and the total PAH concentrations in Oxalis corniculata L. were 251, 346, and 600 mg·kg(-1), respectively. The PCR-DGGE results showed that the endophytic bacterial communities in the roots and shoots of the two plants were quite different, although most bacteria belonged to Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes. A total of 68 endophytic bacterial strains were isolated from different tissues of the two plants and classified into three phyla: Firmicutes, Proteobacteria and Bacteroidetes. In both plants, Bacillus spp. and Pseudomonas spp. were the dominant cultivable populations. With an increase in the PAH pollution level, the diversity and distribution of endophytic bacteria in the two plants changed correspondingly, and the number of cultivable endophytic bacterial strains decreased rapidly. Testing of the isolated endophytic bacteria for tolerance to each type of PAH showed that most isolates could grow well on Luria-Bertani media in the presence of different PAHs, and some isolates were able to grow rapidly on a mineral salt medium with a single PAH as the sole carbon and energy source, indicating that these strains may have the potential to degrade PAHs in plants. This research provides the first insight into the characteristics of endophytic bacterial populations under different PAH pollution levels and provides a species resource for the isolation of PAH-degrading endophytic bacteria.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0083054</identifier><identifier>PMID: 24358247</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Alopecurus aequalis ; Bacteria ; Bacteria - drug effects ; Bacteria - genetics ; Bacteria - growth & development ; Bacteria - isolation & purification ; Bacteriological Techniques ; Biodiversity ; Chemicals ; Cultivation ; Culture media ; DNA, Bacterial - analysis ; DNA, Bacterial - genetics ; Electrophoresis - methods ; Endophytes ; Endophytes - drug effects ; Endophytes - genetics ; Endophytes - growth & development ; Endophytes - isolation & purification ; Environmental science ; Gel electrophoresis ; Magnoliopsida - microbiology ; Media (culture) ; Media (isolation) ; Metabolism ; Microorganisms ; Organic contaminants ; Oxalis corniculata ; Plant communities ; Plant resistance ; Poaceae - microbiology ; Pollution ; Pollution levels ; Polycyclic aromatic hydrocarbons ; Polycyclic Aromatic Hydrocarbons - pharmacology ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Populations ; Prairies ; Roots ; Salts ; Sediment pollution ; Shoots ; Soil contamination ; Soil investigations ; Soil Microbiology ; Soil Pollutants - pharmacology ; Soil sciences ; Soils ; Strains (organisms) ; Studies ; Tissues</subject><ispartof>PloS one, 2013-12, Vol.8 (12), p.e83054</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Peng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Peng et al 2013 Peng et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-d51ded2f001e2fa628de93c399acd5730a0b4efae45e63b884c6b4fd74df87933</citedby><cites>FETCH-LOGICAL-c692t-d51ded2f001e2fa628de93c399acd5730a0b4efae45e63b884c6b4fd74df87933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3866203/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3866203/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24358247$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Balestrini, Raffaella</contributor><creatorcontrib>Peng, Anping</creatorcontrib><creatorcontrib>Liu, Juan</creatorcontrib><creatorcontrib>Gao, Yanzheng</creatorcontrib><creatorcontrib>Chen, Zeyou</creatorcontrib><title>Distribution of endophytic bacteria in Alopecurus aequalis Sobol and Oxalis corniculata L. from soils contaminated by polycyclic aromatic hydrocarbons</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The distributions of endophytic bacteria in Alopecurus aequalis Sobol and Oxalis corniculata L. grown in soils contaminated with different levels of polycyclic aromatic hydrocarbons (PAHs) were investigated with polymerase chain reaction followed by denaturing gradient gel electrophoresis technology (PCR-DGGE) and cultivation methods. Twelve types of PAHs, at concentrations varying from 0.16 to 180 mg·kg(-1), were observed in the roots and shoots of the two plants. The total PAH concentrations in Alopecurus aequalis Sobol obtained from three different PAH-contaminated stations were 184, 197, and 304 mg·kg(-1), and the total PAH concentrations in Oxalis corniculata L. were 251, 346, and 600 mg·kg(-1), respectively. The PCR-DGGE results showed that the endophytic bacterial communities in the roots and shoots of the two plants were quite different, although most bacteria belonged to Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes. A total of 68 endophytic bacterial strains were isolated from different tissues of the two plants and classified into three phyla: Firmicutes, Proteobacteria and Bacteroidetes. In both plants, Bacillus spp. and Pseudomonas spp. were the dominant cultivable populations. With an increase in the PAH pollution level, the diversity and distribution of endophytic bacteria in the two plants changed correspondingly, and the number of cultivable endophytic bacterial strains decreased rapidly. Testing of the isolated endophytic bacteria for tolerance to each type of PAH showed that most isolates could grow well on Luria-Bertani media in the presence of different PAHs, and some isolates were able to grow rapidly on a mineral salt medium with a single PAH as the sole carbon and energy source, indicating that these strains may have the potential to degrade PAHs in plants. This research provides the first insight into the characteristics of endophytic bacterial populations under different PAH pollution levels and provides a species resource for the isolation of PAH-degrading endophytic bacteria.</description><subject>Alopecurus aequalis</subject><subject>Bacteria</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - genetics</subject><subject>Bacteria - growth & development</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteriological Techniques</subject><subject>Biodiversity</subject><subject>Chemicals</subject><subject>Cultivation</subject><subject>Culture media</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>Electrophoresis - methods</subject><subject>Endophytes</subject><subject>Endophytes - drug effects</subject><subject>Endophytes - genetics</subject><subject>Endophytes - growth & development</subject><subject>Endophytes - isolation & purification</subject><subject>Environmental science</subject><subject>Gel electrophoresis</subject><subject>Magnoliopsida - microbiology</subject><subject>Media (culture)</subject><subject>Media (isolation)</subject><subject>Metabolism</subject><subject>Microorganisms</subject><subject>Organic contaminants</subject><subject>Oxalis corniculata</subject><subject>Plant communities</subject><subject>Plant resistance</subject><subject>Poaceae - microbiology</subject><subject>Pollution</subject><subject>Pollution levels</subject><subject>Polycyclic aromatic hydrocarbons</subject><subject>Polycyclic Aromatic Hydrocarbons - pharmacology</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Populations</subject><subject>Prairies</subject><subject>Roots</subject><subject>Salts</subject><subject>Sediment pollution</subject><subject>Shoots</subject><subject>Soil contamination</subject><subject>Soil investigations</subject><subject>Soil Microbiology</subject><subject>Soil Pollutants - pharmacology</subject><subject>Soil sciences</subject><subject>Soils</subject><subject>Strains (organisms)</subject><subject>Studies</subject><subject>Tissues</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12LEzEUhgdR3HX1H4gGBMGL1sxkPjI3Qlm_CoWCq96Gk682JU26yYxs_4i_10w7u7SgIHOR4eQ57zmcNyfLXuZ4mpMmf7_xfXBgpzvv1BRjSnBVPsou85YUk7rA5PHJ_0X2LMYNxhWhdf00uyhKUtGibC6z3x9N7ILhfWe8Q14j5aTfrfedEYiD6FQwgIxDM-t3SvShjwjUbQ_WRHTjubcInETLu0NA-OCM6C10gBZTpIPfouiNHW5cB1vjoFMS8T3aebsXe2FTFUgUDOXWexm8gMC9i8-zJxpsVC_G8yr78fnT9-uvk8Xyy_x6tpiIui26iaxyqWShMc5VoaEuqFQtEaRtQciqIRgwL5UGVVaqJpzSUtS81LIppaZNS8hV9vqou7M-snGkkeVlTSmtCakTMT8S0sOG7YLZQtgzD4YdAj6sGITUvlUMeJG3jdaqplUpheRVC5wTKnldtSQftD6M1Xq-VVIo1wWwZ6LnN86s2cr_YoNtycYk8GYUCP62V7H7R8sjtYLUlXHaJzGxNVGwWdkk3wkmNFHTv1Dpk2prkl9KmxQ_S3h3ljB4qu66FfQxsvnNt_9nlz_P2bcn7FqB7dbR28OLjOdgeQRF8DEGpR8ml2M27MT9NNiwE2zciZT26nTqD0n3S0D-AIZwC5s</recordid><startdate>20131217</startdate><enddate>20131217</enddate><creator>Peng, Anping</creator><creator>Liu, Juan</creator><creator>Gao, Yanzheng</creator><creator>Chen, Zeyou</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131217</creationdate><title>Distribution of endophytic bacteria in Alopecurus aequalis Sobol and Oxalis corniculata L. from soils contaminated by polycyclic aromatic hydrocarbons</title><author>Peng, Anping ; Liu, Juan ; Gao, Yanzheng ; Chen, Zeyou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-d51ded2f001e2fa628de93c399acd5730a0b4efae45e63b884c6b4fd74df87933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Alopecurus aequalis</topic><topic>Bacteria</topic><topic>Bacteria - 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Twelve types of PAHs, at concentrations varying from 0.16 to 180 mg·kg(-1), were observed in the roots and shoots of the two plants. The total PAH concentrations in Alopecurus aequalis Sobol obtained from three different PAH-contaminated stations were 184, 197, and 304 mg·kg(-1), and the total PAH concentrations in Oxalis corniculata L. were 251, 346, and 600 mg·kg(-1), respectively. The PCR-DGGE results showed that the endophytic bacterial communities in the roots and shoots of the two plants were quite different, although most bacteria belonged to Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes. A total of 68 endophytic bacterial strains were isolated from different tissues of the two plants and classified into three phyla: Firmicutes, Proteobacteria and Bacteroidetes. In both plants, Bacillus spp. and Pseudomonas spp. were the dominant cultivable populations. With an increase in the PAH pollution level, the diversity and distribution of endophytic bacteria in the two plants changed correspondingly, and the number of cultivable endophytic bacterial strains decreased rapidly. Testing of the isolated endophytic bacteria for tolerance to each type of PAH showed that most isolates could grow well on Luria-Bertani media in the presence of different PAHs, and some isolates were able to grow rapidly on a mineral salt medium with a single PAH as the sole carbon and energy source, indicating that these strains may have the potential to degrade PAHs in plants. This research provides the first insight into the characteristics of endophytic bacterial populations under different PAH pollution levels and provides a species resource for the isolation of PAH-degrading endophytic bacteria.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24358247</pmid><doi>10.1371/journal.pone.0083054</doi><tpages>e83054</tpages><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1468886336 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Alopecurus aequalis Bacteria Bacteria - drug effects Bacteria - genetics Bacteria - growth & development Bacteria - isolation & purification Bacteriological Techniques Biodiversity Chemicals Cultivation Culture media DNA, Bacterial - analysis DNA, Bacterial - genetics Electrophoresis - methods Endophytes Endophytes - drug effects Endophytes - genetics Endophytes - growth & development Endophytes - isolation & purification Environmental science Gel electrophoresis Magnoliopsida - microbiology Media (culture) Media (isolation) Metabolism Microorganisms Organic contaminants Oxalis corniculata Plant communities Plant resistance Poaceae - microbiology Pollution Pollution levels Polycyclic aromatic hydrocarbons Polycyclic Aromatic Hydrocarbons - pharmacology Polymerase chain reaction Polymerase Chain Reaction - methods Populations Prairies Roots Salts Sediment pollution Shoots Soil contamination Soil investigations Soil Microbiology Soil Pollutants - pharmacology Soil sciences Soils Strains (organisms) Studies Tissues |
| title | Distribution of endophytic bacteria in Alopecurus aequalis Sobol and Oxalis corniculata L. from soils contaminated by polycyclic aromatic hydrocarbons |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T05%3A28%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Distribution%20of%20endophytic%20bacteria%20in%20Alopecurus%20aequalis%20Sobol%20and%20Oxalis%20corniculata%20L.%20from%20soils%20contaminated%20by%20polycyclic%20aromatic%20hydrocarbons&rft.jtitle=PloS%20one&rft.au=Peng,%20Anping&rft.date=2013-12-17&rft.volume=8&rft.issue=12&rft.spage=e83054&rft.pages=e83054-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0083054&rft_dat=%3Cgale_plos_%3EA478243038%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1468886336&rft_id=info:pmid/24358247&rft_galeid=A478243038&rft_doaj_id=oai_doaj_org_article_ab2197ffe6854dcdb59abb38db659316&rfr_iscdi=true |