A trio of viral proteins tunes aphid-plant interactions in Arabidopsis thaliana
Virus-induced deterrence to aphid feeding is believed to promote plant virus transmission by encouraging migration of virus-bearing insects away from infected plants. We investigated the effects of infection by an aphid-transmitted virus, cucumber mosaic virus (CMV), on the interaction of Arabidopsi...
Gespeichert in:
| Veröffentlicht in: | PloS one 2013-12, Vol.8 (12), p.e83066 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 12 |
| container_start_page | e83066 |
| container_title | PloS one |
| container_volume | 8 |
| creator | Westwood, Jack H Groen, Simon C Du, Zhiyou Murphy, Alex M Anggoro, Damar Tri Tungadi, Trisna Luang-In, Vijitra Lewsey, Mathew G Rossiter, John T Powell, Glen Smith, Alison G Carr, John P |
| description | Virus-induced deterrence to aphid feeding is believed to promote plant virus transmission by encouraging migration of virus-bearing insects away from infected plants. We investigated the effects of infection by an aphid-transmitted virus, cucumber mosaic virus (CMV), on the interaction of Arabidopsis thaliana, one of the natural hosts for CMV, with Myzus persicae (common names: 'peach-potato aphid', 'green peach aphid').
Infection of Arabidopsis (ecotype Col-0) with CMV strain Fny (Fny-CMV) induced biosynthesis of the aphid feeding-deterrent 4-methoxy-indol-3-yl-methylglucosinolate (4MI3M). 4MI3M inhibited phloem ingestion by aphids and consequently discouraged aphid settling. The CMV 2b protein is a suppressor of antiviral RNA silencing, which has previously been implicated in altering plant-aphid interactions. Its presence in infected hosts enhances the accumulation of CMV and the other four viral proteins. Another viral gene product, the 2a protein (an RNA-dependent RNA polymerase), triggers defensive signaling, leading to increased 4MI3M accumulation. The 2b protein can inhibit ARGONAUTE1 (AGO1), a host factor that both positively-regulates 4MI3M biosynthesis and negatively-regulates accumulation of substance(s) toxic to aphids. However, the 1a replicase protein moderated 2b-mediated inhibition of AGO1, ensuring that aphids were deterred from feeding but not poisoned. The LS strain of CMV did not induce feeding deterrence in Arabidopsis ecotype Col-0.
Inhibition of AGO1 by the 2b protein could act as a booby trap since this will trigger antibiosis against aphids. However, for Fny-CMV the interplay of three viral proteins (1a, 2a and 2b) appears to balance the need of the virus to inhibit antiviral silencing, while inducing a mild resistance (antixenosis) that is thought to promote transmission. The strain-specific effects of CMV on Arabidopsis-aphid interactions, and differences between the effects of Fny-CMV on this plant and those seen previously in tobacco (inhibition of resistance to aphids) may have important epidemiological consequences. |
| doi_str_mv | 10.1371/journal.pone.0083066 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1467274330</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478330513</galeid><doaj_id>oai_doaj_org_article_ad1084b05b7c496cb8b89c80c29d260c</doaj_id><sourcerecordid>A478330513</sourcerecordid><originalsourceid>FETCH-LOGICAL-c6076-c16f978d985dc10d0e4a25afd61ebca4d76813c76b2be8bcbaa634f1763dbf593</originalsourceid><addsrcrecordid>eNqNkl2L1DAUhoso7rr6D0QLguDFjEnTJunNwrD4MbAw4NdtOPnoTIZOUpN00X9vdqe7TEFBcpFw8pw3Jy9vUbzEaIkJw-_3fgwO-uXgnVkixAmi9FFxjltSLWiFyOOT81nxLMY9Qg3hlD4tzqqa1G1NyHmxWZUpWF_6rryxAfpyCD4Z62KZRmdiCcPO6sXQg0uldckEUMn6fG1duQogrfZDtJneQW_BwfPiSQd9NC-m_aL4_vHDt6vPi-vNp_XV6nqhKGJ0oTDtWsZ1yxutMNLI1FA10GmKjVRQa0Y5JopRWUnDpZIAlNQdZpRo2TUtuSheH3WH3kcxeREFrimrWP4ZysT6SGgPezEEe4DwW3iw4q7gw1ZASFb1RoDGiNcSNZKpuqVKcslbxZGqWl1RpLLW5fTaKA9GK-NS9momOr9xdie2_kYQ3rS0YVngzSQQ_M_RxPSPkSdqC3kq6zqfxdTBRiVWNeMZaTDJ1PIvVF7aHKzKaehsrs8a3s0aMpPMr7SFMUax_vrl_9nNjzn79oTdGejTLvp-vAvIHKyPoAo-xmC6B-cwErdhvndD3IZZTGHOba9OXX9ouk8v-QMoTe-H</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1467274330</pqid></control><display><type>article</type><title>A trio of viral proteins tunes aphid-plant interactions in Arabidopsis thaliana</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Westwood, Jack H ; Groen, Simon C ; Du, Zhiyou ; Murphy, Alex M ; Anggoro, Damar Tri ; Tungadi, Trisna ; Luang-In, Vijitra ; Lewsey, Mathew G ; Rossiter, John T ; Powell, Glen ; Smith, Alison G ; Carr, John P</creator><contributor>Rao, A.L.N.</contributor><creatorcontrib>Westwood, Jack H ; Groen, Simon C ; Du, Zhiyou ; Murphy, Alex M ; Anggoro, Damar Tri ; Tungadi, Trisna ; Luang-In, Vijitra ; Lewsey, Mathew G ; Rossiter, John T ; Powell, Glen ; Smith, Alison G ; Carr, John P ; Rao, A.L.N.</creatorcontrib><description>Virus-induced deterrence to aphid feeding is believed to promote plant virus transmission by encouraging migration of virus-bearing insects away from infected plants. We investigated the effects of infection by an aphid-transmitted virus, cucumber mosaic virus (CMV), on the interaction of Arabidopsis thaliana, one of the natural hosts for CMV, with Myzus persicae (common names: 'peach-potato aphid', 'green peach aphid').
Infection of Arabidopsis (ecotype Col-0) with CMV strain Fny (Fny-CMV) induced biosynthesis of the aphid feeding-deterrent 4-methoxy-indol-3-yl-methylglucosinolate (4MI3M). 4MI3M inhibited phloem ingestion by aphids and consequently discouraged aphid settling. The CMV 2b protein is a suppressor of antiviral RNA silencing, which has previously been implicated in altering plant-aphid interactions. Its presence in infected hosts enhances the accumulation of CMV and the other four viral proteins. Another viral gene product, the 2a protein (an RNA-dependent RNA polymerase), triggers defensive signaling, leading to increased 4MI3M accumulation. The 2b protein can inhibit ARGONAUTE1 (AGO1), a host factor that both positively-regulates 4MI3M biosynthesis and negatively-regulates accumulation of substance(s) toxic to aphids. However, the 1a replicase protein moderated 2b-mediated inhibition of AGO1, ensuring that aphids were deterred from feeding but not poisoned. The LS strain of CMV did not induce feeding deterrence in Arabidopsis ecotype Col-0.
Inhibition of AGO1 by the 2b protein could act as a booby trap since this will trigger antibiosis against aphids. However, for Fny-CMV the interplay of three viral proteins (1a, 2a and 2b) appears to balance the need of the virus to inhibit antiviral silencing, while inducing a mild resistance (antixenosis) that is thought to promote transmission. The strain-specific effects of CMV on Arabidopsis-aphid interactions, and differences between the effects of Fny-CMV on this plant and those seen previously in tobacco (inhibition of resistance to aphids) may have important epidemiological consequences.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0083066</identifier><identifier>PMID: 24349433</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accumulation ; Animals ; Antibiosis ; Antiviral agents ; Aphidoidea ; Aphids ; Aphids - physiology ; Aphids - virology ; Arabidopsis ; Arabidopsis - parasitology ; Arabidopsis - virology ; Arabidopsis thaliana ; Bioinformatics ; Biosynthesis ; Cucumovirus - metabolism ; Disease resistance ; Disease transmission ; DNA-directed RNA polymerase ; Epidemiology ; Feeding ; Feeding Behavior ; Flowers & plants ; Health aspects ; Host-Parasite Interactions ; Infection ; Infections ; Ingestion ; Inhibition ; Insects ; Migration ; Myzus persicae ; Physiological aspects ; Plant sciences ; Plant virus diseases ; Plant viruses ; Plants (botany) ; Plants (Organisms) ; Potatoes ; Process controls ; Proteins ; Replicase ; Ribonucleic acid ; RNA ; RNA-directed RNA polymerase ; RNA-mediated interference ; Signaling ; Studies ; Tobacco ; Viral proteins ; Viral Proteins - metabolism ; Virology ; Viruses</subject><ispartof>PloS one, 2013-12, Vol.8 (12), p.e83066</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Westwood et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Westwood et al 2013 Westwood et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6076-c16f978d985dc10d0e4a25afd61ebca4d76813c76b2be8bcbaa634f1763dbf593</citedby><cites>FETCH-LOGICAL-c6076-c16f978d985dc10d0e4a25afd61ebca4d76813c76b2be8bcbaa634f1763dbf593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3859657/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3859657/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24349433$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Rao, A.L.N.</contributor><creatorcontrib>Westwood, Jack H</creatorcontrib><creatorcontrib>Groen, Simon C</creatorcontrib><creatorcontrib>Du, Zhiyou</creatorcontrib><creatorcontrib>Murphy, Alex M</creatorcontrib><creatorcontrib>Anggoro, Damar Tri</creatorcontrib><creatorcontrib>Tungadi, Trisna</creatorcontrib><creatorcontrib>Luang-In, Vijitra</creatorcontrib><creatorcontrib>Lewsey, Mathew G</creatorcontrib><creatorcontrib>Rossiter, John T</creatorcontrib><creatorcontrib>Powell, Glen</creatorcontrib><creatorcontrib>Smith, Alison G</creatorcontrib><creatorcontrib>Carr, John P</creatorcontrib><title>A trio of viral proteins tunes aphid-plant interactions in Arabidopsis thaliana</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Virus-induced deterrence to aphid feeding is believed to promote plant virus transmission by encouraging migration of virus-bearing insects away from infected plants. We investigated the effects of infection by an aphid-transmitted virus, cucumber mosaic virus (CMV), on the interaction of Arabidopsis thaliana, one of the natural hosts for CMV, with Myzus persicae (common names: 'peach-potato aphid', 'green peach aphid').
Infection of Arabidopsis (ecotype Col-0) with CMV strain Fny (Fny-CMV) induced biosynthesis of the aphid feeding-deterrent 4-methoxy-indol-3-yl-methylglucosinolate (4MI3M). 4MI3M inhibited phloem ingestion by aphids and consequently discouraged aphid settling. The CMV 2b protein is a suppressor of antiviral RNA silencing, which has previously been implicated in altering plant-aphid interactions. Its presence in infected hosts enhances the accumulation of CMV and the other four viral proteins. Another viral gene product, the 2a protein (an RNA-dependent RNA polymerase), triggers defensive signaling, leading to increased 4MI3M accumulation. The 2b protein can inhibit ARGONAUTE1 (AGO1), a host factor that both positively-regulates 4MI3M biosynthesis and negatively-regulates accumulation of substance(s) toxic to aphids. However, the 1a replicase protein moderated 2b-mediated inhibition of AGO1, ensuring that aphids were deterred from feeding but not poisoned. The LS strain of CMV did not induce feeding deterrence in Arabidopsis ecotype Col-0.
Inhibition of AGO1 by the 2b protein could act as a booby trap since this will trigger antibiosis against aphids. However, for Fny-CMV the interplay of three viral proteins (1a, 2a and 2b) appears to balance the need of the virus to inhibit antiviral silencing, while inducing a mild resistance (antixenosis) that is thought to promote transmission. The strain-specific effects of CMV on Arabidopsis-aphid interactions, and differences between the effects of Fny-CMV on this plant and those seen previously in tobacco (inhibition of resistance to aphids) may have important epidemiological consequences.</description><subject>Accumulation</subject><subject>Animals</subject><subject>Antibiosis</subject><subject>Antiviral agents</subject><subject>Aphidoidea</subject><subject>Aphids</subject><subject>Aphids - physiology</subject><subject>Aphids - virology</subject><subject>Arabidopsis</subject><subject>Arabidopsis - parasitology</subject><subject>Arabidopsis - virology</subject><subject>Arabidopsis thaliana</subject><subject>Bioinformatics</subject><subject>Biosynthesis</subject><subject>Cucumovirus - metabolism</subject><subject>Disease resistance</subject><subject>Disease transmission</subject><subject>DNA-directed RNA polymerase</subject><subject>Epidemiology</subject><subject>Feeding</subject><subject>Feeding Behavior</subject><subject>Flowers & plants</subject><subject>Health aspects</subject><subject>Host-Parasite Interactions</subject><subject>Infection</subject><subject>Infections</subject><subject>Ingestion</subject><subject>Inhibition</subject><subject>Insects</subject><subject>Migration</subject><subject>Myzus persicae</subject><subject>Physiological aspects</subject><subject>Plant sciences</subject><subject>Plant virus diseases</subject><subject>Plant viruses</subject><subject>Plants (botany)</subject><subject>Plants (Organisms)</subject><subject>Potatoes</subject><subject>Process controls</subject><subject>Proteins</subject><subject>Replicase</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA-directed RNA polymerase</subject><subject>RNA-mediated interference</subject><subject>Signaling</subject><subject>Studies</subject><subject>Tobacco</subject><subject>Viral proteins</subject><subject>Viral Proteins - metabolism</subject><subject>Virology</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7rr6D0QLguDFjEnTJunNwrD4MbAw4NdtOPnoTIZOUpN00X9vdqe7TEFBcpFw8pw3Jy9vUbzEaIkJw-_3fgwO-uXgnVkixAmi9FFxjltSLWiFyOOT81nxLMY9Qg3hlD4tzqqa1G1NyHmxWZUpWF_6rryxAfpyCD4Z62KZRmdiCcPO6sXQg0uldckEUMn6fG1duQogrfZDtJneQW_BwfPiSQd9NC-m_aL4_vHDt6vPi-vNp_XV6nqhKGJ0oTDtWsZ1yxutMNLI1FA10GmKjVRQa0Y5JopRWUnDpZIAlNQdZpRo2TUtuSheH3WH3kcxeREFrimrWP4ZysT6SGgPezEEe4DwW3iw4q7gw1ZASFb1RoDGiNcSNZKpuqVKcslbxZGqWl1RpLLW5fTaKA9GK-NS9momOr9xdie2_kYQ3rS0YVngzSQQ_M_RxPSPkSdqC3kq6zqfxdTBRiVWNeMZaTDJ1PIvVF7aHKzKaehsrs8a3s0aMpPMr7SFMUax_vrl_9nNjzn79oTdGejTLvp-vAvIHKyPoAo-xmC6B-cwErdhvndD3IZZTGHOba9OXX9ouk8v-QMoTe-H</recordid><startdate>20131211</startdate><enddate>20131211</enddate><creator>Westwood, Jack H</creator><creator>Groen, Simon C</creator><creator>Du, Zhiyou</creator><creator>Murphy, Alex M</creator><creator>Anggoro, Damar Tri</creator><creator>Tungadi, Trisna</creator><creator>Luang-In, Vijitra</creator><creator>Lewsey, Mathew G</creator><creator>Rossiter, John T</creator><creator>Powell, Glen</creator><creator>Smith, Alison G</creator><creator>Carr, John P</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131211</creationdate><title>A trio of viral proteins tunes aphid-plant interactions in Arabidopsis thaliana</title><author>Westwood, Jack H ; Groen, Simon C ; Du, Zhiyou ; Murphy, Alex M ; Anggoro, Damar Tri ; Tungadi, Trisna ; Luang-In, Vijitra ; Lewsey, Mathew G ; Rossiter, John T ; Powell, Glen ; Smith, Alison G ; Carr, John P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6076-c16f978d985dc10d0e4a25afd61ebca4d76813c76b2be8bcbaa634f1763dbf593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Accumulation</topic><topic>Animals</topic><topic>Antibiosis</topic><topic>Antiviral agents</topic><topic>Aphidoidea</topic><topic>Aphids</topic><topic>Aphids - physiology</topic><topic>Aphids - virology</topic><topic>Arabidopsis</topic><topic>Arabidopsis - parasitology</topic><topic>Arabidopsis - virology</topic><topic>Arabidopsis thaliana</topic><topic>Bioinformatics</topic><topic>Biosynthesis</topic><topic>Cucumovirus - metabolism</topic><topic>Disease resistance</topic><topic>Disease transmission</topic><topic>DNA-directed RNA polymerase</topic><topic>Epidemiology</topic><topic>Feeding</topic><topic>Feeding Behavior</topic><topic>Flowers & plants</topic><topic>Health aspects</topic><topic>Host-Parasite Interactions</topic><topic>Infection</topic><topic>Infections</topic><topic>Ingestion</topic><topic>Inhibition</topic><topic>Insects</topic><topic>Migration</topic><topic>Myzus persicae</topic><topic>Physiological aspects</topic><topic>Plant sciences</topic><topic>Plant virus diseases</topic><topic>Plant viruses</topic><topic>Plants (botany)</topic><topic>Plants (Organisms)</topic><topic>Potatoes</topic><topic>Process controls</topic><topic>Proteins</topic><topic>Replicase</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA-directed RNA polymerase</topic><topic>RNA-mediated interference</topic><topic>Signaling</topic><topic>Studies</topic><topic>Tobacco</topic><topic>Viral proteins</topic><topic>Viral Proteins - metabolism</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Westwood, Jack H</creatorcontrib><creatorcontrib>Groen, Simon C</creatorcontrib><creatorcontrib>Du, Zhiyou</creatorcontrib><creatorcontrib>Murphy, Alex M</creatorcontrib><creatorcontrib>Anggoro, Damar Tri</creatorcontrib><creatorcontrib>Tungadi, Trisna</creatorcontrib><creatorcontrib>Luang-In, Vijitra</creatorcontrib><creatorcontrib>Lewsey, Mathew G</creatorcontrib><creatorcontrib>Rossiter, John T</creatorcontrib><creatorcontrib>Powell, Glen</creatorcontrib><creatorcontrib>Smith, Alison G</creatorcontrib><creatorcontrib>Carr, John P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Westwood, Jack H</au><au>Groen, Simon C</au><au>Du, Zhiyou</au><au>Murphy, Alex M</au><au>Anggoro, Damar Tri</au><au>Tungadi, Trisna</au><au>Luang-In, Vijitra</au><au>Lewsey, Mathew G</au><au>Rossiter, John T</au><au>Powell, Glen</au><au>Smith, Alison G</au><au>Carr, John P</au><au>Rao, A.L.N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A trio of viral proteins tunes aphid-plant interactions in Arabidopsis thaliana</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-12-11</date><risdate>2013</risdate><volume>8</volume><issue>12</issue><spage>e83066</spage><pages>e83066-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Virus-induced deterrence to aphid feeding is believed to promote plant virus transmission by encouraging migration of virus-bearing insects away from infected plants. We investigated the effects of infection by an aphid-transmitted virus, cucumber mosaic virus (CMV), on the interaction of Arabidopsis thaliana, one of the natural hosts for CMV, with Myzus persicae (common names: 'peach-potato aphid', 'green peach aphid').
Infection of Arabidopsis (ecotype Col-0) with CMV strain Fny (Fny-CMV) induced biosynthesis of the aphid feeding-deterrent 4-methoxy-indol-3-yl-methylglucosinolate (4MI3M). 4MI3M inhibited phloem ingestion by aphids and consequently discouraged aphid settling. The CMV 2b protein is a suppressor of antiviral RNA silencing, which has previously been implicated in altering plant-aphid interactions. Its presence in infected hosts enhances the accumulation of CMV and the other four viral proteins. Another viral gene product, the 2a protein (an RNA-dependent RNA polymerase), triggers defensive signaling, leading to increased 4MI3M accumulation. The 2b protein can inhibit ARGONAUTE1 (AGO1), a host factor that both positively-regulates 4MI3M biosynthesis and negatively-regulates accumulation of substance(s) toxic to aphids. However, the 1a replicase protein moderated 2b-mediated inhibition of AGO1, ensuring that aphids were deterred from feeding but not poisoned. The LS strain of CMV did not induce feeding deterrence in Arabidopsis ecotype Col-0.
Inhibition of AGO1 by the 2b protein could act as a booby trap since this will trigger antibiosis against aphids. However, for Fny-CMV the interplay of three viral proteins (1a, 2a and 2b) appears to balance the need of the virus to inhibit antiviral silencing, while inducing a mild resistance (antixenosis) that is thought to promote transmission. The strain-specific effects of CMV on Arabidopsis-aphid interactions, and differences between the effects of Fny-CMV on this plant and those seen previously in tobacco (inhibition of resistance to aphids) may have important epidemiological consequences.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24349433</pmid><doi>10.1371/journal.pone.0083066</doi><tpages>e83066</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-12, Vol.8 (12), p.e83066 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1467274330 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Accumulation Animals Antibiosis Antiviral agents Aphidoidea Aphids Aphids - physiology Aphids - virology Arabidopsis Arabidopsis - parasitology Arabidopsis - virology Arabidopsis thaliana Bioinformatics Biosynthesis Cucumovirus - metabolism Disease resistance Disease transmission DNA-directed RNA polymerase Epidemiology Feeding Feeding Behavior Flowers & plants Health aspects Host-Parasite Interactions Infection Infections Ingestion Inhibition Insects Migration Myzus persicae Physiological aspects Plant sciences Plant virus diseases Plant viruses Plants (botany) Plants (Organisms) Potatoes Process controls Proteins Replicase Ribonucleic acid RNA RNA-directed RNA polymerase RNA-mediated interference Signaling Studies Tobacco Viral proteins Viral Proteins - metabolism Virology Viruses |
| title | A trio of viral proteins tunes aphid-plant interactions in Arabidopsis thaliana |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T13%3A46%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20trio%20of%20viral%20proteins%20tunes%20aphid-plant%20interactions%20in%20Arabidopsis%20thaliana&rft.jtitle=PloS%20one&rft.au=Westwood,%20Jack%20H&rft.date=2013-12-11&rft.volume=8&rft.issue=12&rft.spage=e83066&rft.pages=e83066-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0083066&rft_dat=%3Cgale_plos_%3EA478330513%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1467274330&rft_id=info:pmid/24349433&rft_galeid=A478330513&rft_doaj_id=oai_doaj_org_article_ad1084b05b7c496cb8b89c80c29d260c&rfr_iscdi=true |