Study on the absorbed fingerprint-efficacy of yuanhu zhitong tablet based on chemical analysis, vasorelaxation evaluation and data mining
Yuanhu Zhitong Tablet (YZT) is an example of a typical and relatively simple clinical herb formula that is widely used in clinics. It is generally believed that YZT play a therapeutical effect in vivo by the synergism of multiple constituents. Thus, it is necessary to build the relationship between...
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| Veröffentlicht in: | PloS one 2013-12, Vol.8 (12), p.e81135 |
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| creator | Xu, Haiyu Li, Ke Chen, Yanjun Zhang, Yingchun Tang, Shihuan Wang, Shanshan Shen, Dan Wang, Xuguang Lei, Yun Li, Defeng Zhang, Yi Jin, Lan Yang, Hongjun Huang, Luqi |
| description | Yuanhu Zhitong Tablet (YZT) is an example of a typical and relatively simple clinical herb formula that is widely used in clinics. It is generally believed that YZT play a therapeutical effect in vivo by the synergism of multiple constituents. Thus, it is necessary to build the relationship between the absorbed fingerprints and bioactivity so as to ensure the quality, safety and efficacy. In this study, a new combinative method, an intestinal absorption test coupled with a vasorelaxation bioactivity experiment in vitro, was a simple, sensitive, and feasible technique to study on the absorbed fingerprint-efficacy of YZT based on chemical analysis, vasorelaxation evaluation and data mining. As part of this method, an everted intestinal sac method was performed to determine the intestinal absorption of YZT solutions. YZT were dissolved in solution (n = 12), and the portion of the solution that was absorbed into intestinal sacs was analyzed using rapid-resolution liquid chromatography coupled with quadruple time-of-flight mass spectrometry (RRLC-Q-TOF/MS). Semi-quantitative analysis indicated the presence of 34 compounds. The effect of the intestinally absorbed solution on vasorelaxation of rat aortic rings with endothelium attached was then evaluated in vitro. The results showed that samples grouped by HCA from chemical profiles have similar bioactivity while samples in different groups displayed very different. Moreover, it established a relationship between the absorbed fingerprints and their bioactivity to identify important components by grey relational analysis, which could predict bioactive values based on chemical profiles and provide an evidence for the quantification of multi-constituents. |
| doi_str_mv | 10.1371/journal.pone.0081135 |
| format | Article |
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It is generally believed that YZT play a therapeutical effect in vivo by the synergism of multiple constituents. Thus, it is necessary to build the relationship between the absorbed fingerprints and bioactivity so as to ensure the quality, safety and efficacy. In this study, a new combinative method, an intestinal absorption test coupled with a vasorelaxation bioactivity experiment in vitro, was a simple, sensitive, and feasible technique to study on the absorbed fingerprint-efficacy of YZT based on chemical analysis, vasorelaxation evaluation and data mining. As part of this method, an everted intestinal sac method was performed to determine the intestinal absorption of YZT solutions. YZT were dissolved in solution (n = 12), and the portion of the solution that was absorbed into intestinal sacs was analyzed using rapid-resolution liquid chromatography coupled with quadruple time-of-flight mass spectrometry (RRLC-Q-TOF/MS). Semi-quantitative analysis indicated the presence of 34 compounds. The effect of the intestinally absorbed solution on vasorelaxation of rat aortic rings with endothelium attached was then evaluated in vitro. The results showed that samples grouped by HCA from chemical profiles have similar bioactivity while samples in different groups displayed very different. Moreover, it established a relationship between the absorbed fingerprints and their bioactivity to identify important components by grey relational analysis, which could predict bioactive values based on chemical profiles and provide an evidence for the quantification of multi-constituents.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0081135</identifier><identifier>PMID: 24339904</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Absorption ; Analysis ; Animals ; Aorta ; Bioassays ; Biochemistry ; Biological activity ; Biological assays ; Chemical analysis ; Chromatography ; Cluster Analysis ; Constituents ; Coronary vessels ; Corydalis ; Data Mining ; Data processing ; Drugs, Chinese Herbal - chemistry ; Drugs, Chinese Herbal - metabolism ; Drugs, Chinese Herbal - pharmacology ; Effectiveness ; Endothelium ; Evaluation ; Feasibility studies ; Fingerprints ; Headaches ; In Vitro Techniques ; In vivo methods and tests ; Intestinal Absorption ; Intestine ; Laboratories ; Ligusticum ; Liquid chromatography ; Male ; Mass Spectrometry ; Mass spectroscopy ; Medical research ; Migraine ; Pain ; Professional baseball ; Quality control ; Quantitative analysis ; Rats ; Rats, Sprague-Dawley ; Researchers ; Scientific imaging ; Studies ; Synergism ; Tablets ; Vasodilation ; Vasodilation - drug effects</subject><ispartof>PloS one, 2013-12, Vol.8 (12), p.e81135</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Haiyu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/3.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Haiyu et al 2013 Haiyu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5685-463552ded83a870b1c87c931b428f95b116cdbee117583457cc4f2f2bfae9f783</citedby><cites>FETCH-LOGICAL-c5685-463552ded83a870b1c87c931b428f95b116cdbee117583457cc4f2f2bfae9f783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858225/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858225/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24339904$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Haiyu</creatorcontrib><creatorcontrib>Li, Ke</creatorcontrib><creatorcontrib>Chen, Yanjun</creatorcontrib><creatorcontrib>Zhang, Yingchun</creatorcontrib><creatorcontrib>Tang, Shihuan</creatorcontrib><creatorcontrib>Wang, Shanshan</creatorcontrib><creatorcontrib>Shen, Dan</creatorcontrib><creatorcontrib>Wang, Xuguang</creatorcontrib><creatorcontrib>Lei, Yun</creatorcontrib><creatorcontrib>Li, Defeng</creatorcontrib><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>Jin, Lan</creatorcontrib><creatorcontrib>Yang, Hongjun</creatorcontrib><creatorcontrib>Huang, Luqi</creatorcontrib><title>Study on the absorbed fingerprint-efficacy of yuanhu zhitong tablet based on chemical analysis, vasorelaxation evaluation and data mining</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Yuanhu Zhitong Tablet (YZT) is an example of a typical and relatively simple clinical herb formula that is widely used in clinics. It is generally believed that YZT play a therapeutical effect in vivo by the synergism of multiple constituents. Thus, it is necessary to build the relationship between the absorbed fingerprints and bioactivity so as to ensure the quality, safety and efficacy. In this study, a new combinative method, an intestinal absorption test coupled with a vasorelaxation bioactivity experiment in vitro, was a simple, sensitive, and feasible technique to study on the absorbed fingerprint-efficacy of YZT based on chemical analysis, vasorelaxation evaluation and data mining. As part of this method, an everted intestinal sac method was performed to determine the intestinal absorption of YZT solutions. YZT were dissolved in solution (n = 12), and the portion of the solution that was absorbed into intestinal sacs was analyzed using rapid-resolution liquid chromatography coupled with quadruple time-of-flight mass spectrometry (RRLC-Q-TOF/MS). Semi-quantitative analysis indicated the presence of 34 compounds. The effect of the intestinally absorbed solution on vasorelaxation of rat aortic rings with endothelium attached was then evaluated in vitro. The results showed that samples grouped by HCA from chemical profiles have similar bioactivity while samples in different groups displayed very different. Moreover, it established a relationship between the absorbed fingerprints and their bioactivity to identify important components by grey relational analysis, which could predict bioactive values based on chemical profiles and provide an evidence for the quantification of multi-constituents.</description><subject>Absorption</subject><subject>Analysis</subject><subject>Animals</subject><subject>Aorta</subject><subject>Bioassays</subject><subject>Biochemistry</subject><subject>Biological activity</subject><subject>Biological assays</subject><subject>Chemical analysis</subject><subject>Chromatography</subject><subject>Cluster Analysis</subject><subject>Constituents</subject><subject>Coronary vessels</subject><subject>Corydalis</subject><subject>Data Mining</subject><subject>Data processing</subject><subject>Drugs, Chinese Herbal - chemistry</subject><subject>Drugs, Chinese Herbal - metabolism</subject><subject>Drugs, Chinese Herbal - pharmacology</subject><subject>Effectiveness</subject><subject>Endothelium</subject><subject>Evaluation</subject><subject>Feasibility studies</subject><subject>Fingerprints</subject><subject>Headaches</subject><subject>In Vitro Techniques</subject><subject>In vivo methods and tests</subject><subject>Intestinal Absorption</subject><subject>Intestine</subject><subject>Laboratories</subject><subject>Ligusticum</subject><subject>Liquid chromatography</subject><subject>Male</subject><subject>Mass Spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical research</subject><subject>Migraine</subject><subject>Pain</subject><subject>Professional baseball</subject><subject>Quality control</subject><subject>Quantitative analysis</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Researchers</subject><subject>Scientific imaging</subject><subject>Studies</subject><subject>Synergism</subject><subject>Tablets</subject><subject>Vasodilation</subject><subject>Vasodilation - 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It is generally believed that YZT play a therapeutical effect in vivo by the synergism of multiple constituents. Thus, it is necessary to build the relationship between the absorbed fingerprints and bioactivity so as to ensure the quality, safety and efficacy. In this study, a new combinative method, an intestinal absorption test coupled with a vasorelaxation bioactivity experiment in vitro, was a simple, sensitive, and feasible technique to study on the absorbed fingerprint-efficacy of YZT based on chemical analysis, vasorelaxation evaluation and data mining. As part of this method, an everted intestinal sac method was performed to determine the intestinal absorption of YZT solutions. YZT were dissolved in solution (n = 12), and the portion of the solution that was absorbed into intestinal sacs was analyzed using rapid-resolution liquid chromatography coupled with quadruple time-of-flight mass spectrometry (RRLC-Q-TOF/MS). Semi-quantitative analysis indicated the presence of 34 compounds. The effect of the intestinally absorbed solution on vasorelaxation of rat aortic rings with endothelium attached was then evaluated in vitro. The results showed that samples grouped by HCA from chemical profiles have similar bioactivity while samples in different groups displayed very different. Moreover, it established a relationship between the absorbed fingerprints and their bioactivity to identify important components by grey relational analysis, which could predict bioactive values based on chemical profiles and provide an evidence for the quantification of multi-constituents.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24339904</pmid><doi>10.1371/journal.pone.0081135</doi><tpages>e81135</tpages><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1466544297 |
| source | MEDLINE; NCBI_PubMed Central（免费）; DOAJ Directory of Open Access Journals; PLoS - Public Library of Sciencem (Open Access); Elektronische Zeitschriftenbibliothek - Freely accessible e-journals; Free Full-Text Journals in Chemistry |
| subjects | Absorption Analysis Animals Aorta Bioassays Biochemistry Biological activity Biological assays Chemical analysis Chromatography Cluster Analysis Constituents Coronary vessels Corydalis Data Mining Data processing Drugs, Chinese Herbal - chemistry Drugs, Chinese Herbal - metabolism Drugs, Chinese Herbal - pharmacology Effectiveness Endothelium Evaluation Feasibility studies Fingerprints Headaches In Vitro Techniques In vivo methods and tests Intestinal Absorption Intestine Laboratories Ligusticum Liquid chromatography Male Mass Spectrometry Mass spectroscopy Medical research Migraine Pain Professional baseball Quality control Quantitative analysis Rats Rats, Sprague-Dawley Researchers Scientific imaging Studies Synergism Tablets Vasodilation Vasodilation - drug effects |
| title | Study on the absorbed fingerprint-efficacy of yuanhu zhitong tablet based on chemical analysis, vasorelaxation evaluation and data mining |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T14%3A47%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Study%20on%20the%20absorbed%20fingerprint-efficacy%20of%20yuanhu%20zhitong%20tablet%20based%20on%20chemical%20analysis,%20vasorelaxation%20evaluation%20and%20data%20mining&rft.jtitle=PloS%20one&rft.au=Xu,%20Haiyu&rft.date=2013-12-10&rft.volume=8&rft.issue=12&rft.spage=e81135&rft.pages=e81135-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0081135&rft_dat=%3Cgale_plos_%3EA478345533%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1466544297&rft_id=info:pmid/24339904&rft_galeid=A478345533&rft_doaj_id=oai_doaj_org_article_e494fdc4f34146f1828325469ba37cd5&rfr_iscdi=true |