Transcriptional reprogramming of wheat and the hemibiotrophic pathogen Septoria tritici during two phases of the compatible interaction
The disease septoria leaf blotch of wheat, caused by fungal pathogen Septoria tritici, is of worldwide concern. The fungus exhibits a hemibiotrophic lifestyle, with a long symptomless, biotrophic phase followed by a sudden transition to necrotrophy associated with host necrosis. Little is known abou...
Gespeichert in:
Veröffentlicht in: | PloS one 2013-11, Vol.8 (11), p.e81606 |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | |
---|---|
container_issue | 11 |
container_start_page | e81606 |
container_title | PloS one |
container_volume | 8 |
creator | Yang, Fen Li, Wanshun Jørgensen, Hans J L |
description | The disease septoria leaf blotch of wheat, caused by fungal pathogen Septoria tritici, is of worldwide concern. The fungus exhibits a hemibiotrophic lifestyle, with a long symptomless, biotrophic phase followed by a sudden transition to necrotrophy associated with host necrosis. Little is known about the systematic interaction between fungal pathogenicity and host responses at specific growth stages and the factors triggering the transition. In order to gain some insights into global transcriptome alterations in both host and pathogen during the two phases of the compatible interaction, disease transition was monitored using pathogenesis-related gene markers and H2O2 signature prior to RNA-Seq. Transcriptome analysis revealed that the slow symptomless growth was accompanied by minor metabolic responses and slightly suppressed defences in the host, whereas necrotrophic growth was associated with enhanced host responses involving energy metabolism, transport, signalling, defence and oxidative stress as well as a decrease in photosynthesis. The fungus expresses distinct classes of stage-specific genes encoding potential effectors, probably first suppressing plant defence responses/facilitating the symptomless growth and later triggering life style transition and inducing host necrosis/facilitating the necrotrophic growth. Transport, signalling, anti-oxidative stress mechanisms and apoplastic nutrient acquisition play important roles in the entire infection process of S. tritici. Our findings uncover systematic S. tritici-induced expression profiles of wheat related to specific fungal infection strategies and provide a transcriptome resource for studying both hosts and pathogens in plant-Dothideomycete interactions. |
doi_str_mv | 10.1371/journal.pone.0081606 |
format | Article |
fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_1461961857</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_e7b482016c42484ebc1d9b0be465b23a</doaj_id><sourcerecordid>3138466151</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-94ffe5404a6b5a2b8b8a5647a71fe9db91e900e97cd50e9081a95a574d7e0043</originalsourceid><addsrcrecordid>eNp1ks9u1DAQxiMEoqXwBggs9byLHf9JckFCVYFKlTiwd2vsTDZeJXGwvVR9Al4bh02r9sBpLM98vxmPv6J4z-iW8Yp9OvhjmGDYzn7CLaU1U1S9KM5Zw8uNKil_-eR8VryJ8UCp5LVSr4uzUnDKqazOiz-7AFO0wc3J-YwjAefg9wHG0U174jty1yMkAlNLUo-kx9EZ51Pwc-8smSH1fo8T-Ylz8sEBScElZx1pj2EBpDtP5h4ixoW1EKwfs8qZAYmbEgawS-e3xasOhojv1nhR7L5e766-b25_fLu5-nK7sbJUadOIrkMpqABlJJSmNjVIJSqoWIdNaxqGDaXYVLaVOeStQCNBVqKtkFLBL4qPJ-w8-KjXFUbNhGKNYrWscsXNqaL1cNBzcCOEe-3B6X8XPuw1hPzCATVWRtQlZcqKUtQCjWVtY6hBoaQpOWTW57Xb0YzYWpxSgOEZ9Hlmcr3e-9-a14Llz8uAyxUQ_K8jxvSfkcWpygYfY8DusQOjevHKg0ovXtGrV7Lsw9PpHkUP5uB_AfiVwTU</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1461961857</pqid></control><display><type>article</type><title>Transcriptional reprogramming of wheat and the hemibiotrophic pathogen Septoria tritici during two phases of the compatible interaction</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Yang, Fen ; Li, Wanshun ; Jørgensen, Hans J L</creator><contributor>Lee, Yong-Hwan</contributor><creatorcontrib>Yang, Fen ; Li, Wanshun ; Jørgensen, Hans J L ; Lee, Yong-Hwan</creatorcontrib><description>The disease septoria leaf blotch of wheat, caused by fungal pathogen Septoria tritici, is of worldwide concern. The fungus exhibits a hemibiotrophic lifestyle, with a long symptomless, biotrophic phase followed by a sudden transition to necrotrophy associated with host necrosis. Little is known about the systematic interaction between fungal pathogenicity and host responses at specific growth stages and the factors triggering the transition. In order to gain some insights into global transcriptome alterations in both host and pathogen during the two phases of the compatible interaction, disease transition was monitored using pathogenesis-related gene markers and H2O2 signature prior to RNA-Seq. Transcriptome analysis revealed that the slow symptomless growth was accompanied by minor metabolic responses and slightly suppressed defences in the host, whereas necrotrophic growth was associated with enhanced host responses involving energy metabolism, transport, signalling, defence and oxidative stress as well as a decrease in photosynthesis. The fungus expresses distinct classes of stage-specific genes encoding potential effectors, probably first suppressing plant defence responses/facilitating the symptomless growth and later triggering life style transition and inducing host necrosis/facilitating the necrotrophic growth. Transport, signalling, anti-oxidative stress mechanisms and apoplastic nutrient acquisition play important roles in the entire infection process of S. tritici. Our findings uncover systematic S. tritici-induced expression profiles of wheat related to specific fungal infection strategies and provide a transcriptome resource for studying both hosts and pathogens in plant-Dothideomycete interactions.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0081606</identifier><identifier>PMID: 24303057</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Apoptosis ; Ascomycota - genetics ; Biomass ; Cluster Analysis ; Energy metabolism ; Environmental science ; Fungi ; Gangrene ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Fungal ; Gene Expression Regulation, Plant ; Genomes ; Host-Pathogen Interactions - genetics ; Hydrogen peroxide ; Hydrogen Peroxide - metabolism ; Infections ; Kinases ; Leaf blotch ; Metabolism ; Necrosis ; Nutrient transport ; Oxidative stress ; Pathogenesis ; Pathogenicity ; Pathogens ; Photosynthesis ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Plant Leaves - genetics ; Plant Leaves - metabolism ; Plant Leaves - microbiology ; Proteins ; Ribonucleic acid ; RNA ; Septoria tritici ; Septoria tritici blotch ; Signal transduction ; Signaling ; Studies ; Transcription ; Transcriptome ; Triticum - genetics ; Triticum - metabolism ; Triticum - microbiology ; Wheat</subject><ispartof>PloS one, 2013-11, Vol.8 (11), p.e81606</ispartof><rights>2013 Yang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/3.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Yang et al 2013 Yang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-94ffe5404a6b5a2b8b8a5647a71fe9db91e900e97cd50e9081a95a574d7e0043</citedby><cites>FETCH-LOGICAL-c526t-94ffe5404a6b5a2b8b8a5647a71fe9db91e900e97cd50e9081a95a574d7e0043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841193/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841193/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24303057$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lee, Yong-Hwan</contributor><creatorcontrib>Yang, Fen</creatorcontrib><creatorcontrib>Li, Wanshun</creatorcontrib><creatorcontrib>Jørgensen, Hans J L</creatorcontrib><title>Transcriptional reprogramming of wheat and the hemibiotrophic pathogen Septoria tritici during two phases of the compatible interaction</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The disease septoria leaf blotch of wheat, caused by fungal pathogen Septoria tritici, is of worldwide concern. The fungus exhibits a hemibiotrophic lifestyle, with a long symptomless, biotrophic phase followed by a sudden transition to necrotrophy associated with host necrosis. Little is known about the systematic interaction between fungal pathogenicity and host responses at specific growth stages and the factors triggering the transition. In order to gain some insights into global transcriptome alterations in both host and pathogen during the two phases of the compatible interaction, disease transition was monitored using pathogenesis-related gene markers and H2O2 signature prior to RNA-Seq. Transcriptome analysis revealed that the slow symptomless growth was accompanied by minor metabolic responses and slightly suppressed defences in the host, whereas necrotrophic growth was associated with enhanced host responses involving energy metabolism, transport, signalling, defence and oxidative stress as well as a decrease in photosynthesis. The fungus expresses distinct classes of stage-specific genes encoding potential effectors, probably first suppressing plant defence responses/facilitating the symptomless growth and later triggering life style transition and inducing host necrosis/facilitating the necrotrophic growth. Transport, signalling, anti-oxidative stress mechanisms and apoplastic nutrient acquisition play important roles in the entire infection process of S. tritici. Our findings uncover systematic S. tritici-induced expression profiles of wheat related to specific fungal infection strategies and provide a transcriptome resource for studying both hosts and pathogens in plant-Dothideomycete interactions.</description><subject>Apoptosis</subject><subject>Ascomycota - genetics</subject><subject>Biomass</subject><subject>Cluster Analysis</subject><subject>Energy metabolism</subject><subject>Environmental science</subject><subject>Fungi</subject><subject>Gangrene</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genomes</subject><subject>Host-Pathogen Interactions - genetics</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Infections</subject><subject>Kinases</subject><subject>Leaf blotch</subject><subject>Metabolism</subject><subject>Necrosis</subject><subject>Nutrient transport</subject><subject>Oxidative stress</subject><subject>Pathogenesis</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Photosynthesis</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Leaves - genetics</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Leaves - microbiology</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Septoria tritici</subject><subject>Septoria tritici blotch</subject><subject>Signal transduction</subject><subject>Signaling</subject><subject>Studies</subject><subject>Transcription</subject><subject>Transcriptome</subject><subject>Triticum - genetics</subject><subject>Triticum - metabolism</subject><subject>Triticum - microbiology</subject><subject>Wheat</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNp1ks9u1DAQxiMEoqXwBggs9byLHf9JckFCVYFKlTiwd2vsTDZeJXGwvVR9Al4bh02r9sBpLM98vxmPv6J4z-iW8Yp9OvhjmGDYzn7CLaU1U1S9KM5Zw8uNKil_-eR8VryJ8UCp5LVSr4uzUnDKqazOiz-7AFO0wc3J-YwjAefg9wHG0U174jty1yMkAlNLUo-kx9EZ51Pwc-8smSH1fo8T-Ylz8sEBScElZx1pj2EBpDtP5h4ixoW1EKwfs8qZAYmbEgawS-e3xasOhojv1nhR7L5e766-b25_fLu5-nK7sbJUadOIrkMpqABlJJSmNjVIJSqoWIdNaxqGDaXYVLaVOeStQCNBVqKtkFLBL4qPJ-w8-KjXFUbNhGKNYrWscsXNqaL1cNBzcCOEe-3B6X8XPuw1hPzCATVWRtQlZcqKUtQCjWVtY6hBoaQpOWTW57Xb0YzYWpxSgOEZ9Hlmcr3e-9-a14Llz8uAyxUQ_K8jxvSfkcWpygYfY8DusQOjevHKg0ovXtGrV7Lsw9PpHkUP5uB_AfiVwTU</recordid><startdate>20131126</startdate><enddate>20131126</enddate><creator>Yang, Fen</creator><creator>Li, Wanshun</creator><creator>Jørgensen, Hans J L</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131126</creationdate><title>Transcriptional reprogramming of wheat and the hemibiotrophic pathogen Septoria tritici during two phases of the compatible interaction</title><author>Yang, Fen ; Li, Wanshun ; Jørgensen, Hans J L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-94ffe5404a6b5a2b8b8a5647a71fe9db91e900e97cd50e9081a95a574d7e0043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Apoptosis</topic><topic>Ascomycota - genetics</topic><topic>Biomass</topic><topic>Cluster Analysis</topic><topic>Energy metabolism</topic><topic>Environmental science</topic><topic>Fungi</topic><topic>Gangrene</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genomes</topic><topic>Host-Pathogen Interactions - genetics</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Infections</topic><topic>Kinases</topic><topic>Leaf blotch</topic><topic>Metabolism</topic><topic>Necrosis</topic><topic>Nutrient transport</topic><topic>Oxidative stress</topic><topic>Pathogenesis</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Photosynthesis</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Leaves - genetics</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Leaves - microbiology</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Septoria tritici</topic><topic>Septoria tritici blotch</topic><topic>Signal transduction</topic><topic>Signaling</topic><topic>Studies</topic><topic>Transcription</topic><topic>Transcriptome</topic><topic>Triticum - genetics</topic><topic>Triticum - metabolism</topic><topic>Triticum - microbiology</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Fen</creatorcontrib><creatorcontrib>Li, Wanshun</creatorcontrib><creatorcontrib>Jørgensen, Hans J L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Fen</au><au>Li, Wanshun</au><au>Jørgensen, Hans J L</au><au>Lee, Yong-Hwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptional reprogramming of wheat and the hemibiotrophic pathogen Septoria tritici during two phases of the compatible interaction</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-11-26</date><risdate>2013</risdate><volume>8</volume><issue>11</issue><spage>e81606</spage><pages>e81606-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The disease septoria leaf blotch of wheat, caused by fungal pathogen Septoria tritici, is of worldwide concern. The fungus exhibits a hemibiotrophic lifestyle, with a long symptomless, biotrophic phase followed by a sudden transition to necrotrophy associated with host necrosis. Little is known about the systematic interaction between fungal pathogenicity and host responses at specific growth stages and the factors triggering the transition. In order to gain some insights into global transcriptome alterations in both host and pathogen during the two phases of the compatible interaction, disease transition was monitored using pathogenesis-related gene markers and H2O2 signature prior to RNA-Seq. Transcriptome analysis revealed that the slow symptomless growth was accompanied by minor metabolic responses and slightly suppressed defences in the host, whereas necrotrophic growth was associated with enhanced host responses involving energy metabolism, transport, signalling, defence and oxidative stress as well as a decrease in photosynthesis. The fungus expresses distinct classes of stage-specific genes encoding potential effectors, probably first suppressing plant defence responses/facilitating the symptomless growth and later triggering life style transition and inducing host necrosis/facilitating the necrotrophic growth. Transport, signalling, anti-oxidative stress mechanisms and apoplastic nutrient acquisition play important roles in the entire infection process of S. tritici. Our findings uncover systematic S. tritici-induced expression profiles of wheat related to specific fungal infection strategies and provide a transcriptome resource for studying both hosts and pathogens in plant-Dothideomycete interactions.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24303057</pmid><doi>10.1371/journal.pone.0081606</doi><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1932-6203 |
ispartof | PloS one, 2013-11, Vol.8 (11), p.e81606 |
issn | 1932-6203 1932-6203 |
language | eng |
recordid | cdi_plos_journals_1461961857 |
source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
subjects | Apoptosis Ascomycota - genetics Biomass Cluster Analysis Energy metabolism Environmental science Fungi Gangrene Gene expression Gene Expression Profiling Gene Expression Regulation, Fungal Gene Expression Regulation, Plant Genomes Host-Pathogen Interactions - genetics Hydrogen peroxide Hydrogen Peroxide - metabolism Infections Kinases Leaf blotch Metabolism Necrosis Nutrient transport Oxidative stress Pathogenesis Pathogenicity Pathogens Photosynthesis Plant Diseases - genetics Plant Diseases - microbiology Plant Leaves - genetics Plant Leaves - metabolism Plant Leaves - microbiology Proteins Ribonucleic acid RNA Septoria tritici Septoria tritici blotch Signal transduction Signaling Studies Transcription Transcriptome Triticum - genetics Triticum - metabolism Triticum - microbiology Wheat |
title | Transcriptional reprogramming of wheat and the hemibiotrophic pathogen Septoria tritici during two phases of the compatible interaction |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T01%3A02%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transcriptional%20reprogramming%20of%20wheat%20and%20the%20hemibiotrophic%20pathogen%20Septoria%20tritici%20during%20two%20phases%20of%20the%20compatible%20interaction&rft.jtitle=PloS%20one&rft.au=Yang,%20Fen&rft.date=2013-11-26&rft.volume=8&rft.issue=11&rft.spage=e81606&rft.pages=e81606-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0081606&rft_dat=%3Cproquest_plos_%3E3138466151%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1461961857&rft_id=info:pmid/24303057&rft_doaj_id=oai_doaj_org_article_e7b482016c42484ebc1d9b0be465b23a&rfr_iscdi=true |