λ Phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo
Using phages is a novel field of cancer therapy and phage nanobioparticles (NBPs) such as λ phage could be modified to deliver and express genetic cassettes into eukaryotic cells safely in contrast with animal viruses. Apoptin, a protein from chicken anemia virus (CAV) has the ability to specificall...
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| Veröffentlicht in: | PloS one 2013-11, Vol.8 (11), p.e79907 |
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| creator | Shoae-Hassani, Alireza Keyhanvar, Peyman Seifalian, Alexander Marcus Mortazavi-Tabatabaei, Seyed Abdolreza Ghaderi, Narmin Issazadeh, Khosro Amirmozafari, Nour Verdi, Javad |
| description | Using phages is a novel field of cancer therapy and phage nanobioparticles (NBPs) such as λ phage could be modified to deliver and express genetic cassettes into eukaryotic cells safely in contrast with animal viruses. Apoptin, a protein from chicken anemia virus (CAV) has the ability to specifically induce apoptosis only in carcinoma cells. We presented a safe method of breast tumor therapy via the apoptin expressing λ NBPs. Here, we constructed a λ ZAP-CMV-apoptin recombinant NBP and investigated the effectiveness of its apoptotic activity on BT-474, MDA-MB-361, SKBR-3, UACC-812 and ZR-75 cell lines that over-expressing her-2 marker. Apoptosis was evaluated via annexin-V fluorescent iso-thiocyanate/propidium iodide staining, flow-cytometric method and TUNEL assay. Transfection with NBPs carrying λ ZAP-CMV-apoptin significantly inhibited growth of all the breast carcinoma cell lines in vitro. Also nude mice model implanted BT-474 human breast tumor was successfully responded to the systemic and local injection of untargeted recombinant λ NBPs. The results presented here reveal important features of recombinant λ nanobioparticles to serve as safe delivery and expression platform for human cancer therapy. |
| doi_str_mv | 10.1371/journal.pone.0079907 |
| format | Article |
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Apoptin, a protein from chicken anemia virus (CAV) has the ability to specifically induce apoptosis only in carcinoma cells. We presented a safe method of breast tumor therapy via the apoptin expressing λ NBPs. Here, we constructed a λ ZAP-CMV-apoptin recombinant NBP and investigated the effectiveness of its apoptotic activity on BT-474, MDA-MB-361, SKBR-3, UACC-812 and ZR-75 cell lines that over-expressing her-2 marker. Apoptosis was evaluated via annexin-V fluorescent iso-thiocyanate/propidium iodide staining, flow-cytometric method and TUNEL assay. Transfection with NBPs carrying λ ZAP-CMV-apoptin significantly inhibited growth of all the breast carcinoma cell lines in vitro. Also nude mice model implanted BT-474 human breast tumor was successfully responded to the systemic and local injection of untargeted recombinant λ NBPs. The results presented here reveal important features of recombinant λ nanobioparticles to serve as safe delivery and expression platform for human cancer therapy.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0079907</identifier><identifier>PMID: 24278212</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Anemia ; Animals ; Antineoplastic Agents ; Apoptosis ; Bacteriophage lambda - genetics ; Biotechnology ; Breast ; Breast cancer ; Breast carcinoma ; Breast Neoplasms - pathology ; Cancer ; Cancer therapies ; Capsid Proteins - genetics ; Cassettes ; Cell Division - genetics ; ErbB-2 protein ; Female ; Flow Cytometry ; Fluorescence ; Humans ; Iodides ; Kinases ; Mice ; Mice, Nude ; Nanoparticles ; Phages ; Propidium iodide ; Recombinant Proteins - genetics ; Science ; Surgical implants ; Therapy ; Transfection ; Tumor cell lines ; Viruses</subject><ispartof>PloS one, 2013-11, Vol.8 (11), p.e79907</ispartof><rights>2013 Shoae-Hassani et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/3.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Shoae-Hassani et al 2013 Shoae-Hassani et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-20cb171cdd968b328b67ab431b7c95c2ed5a6484e28c0b35a4891c199503a3073</citedby><cites>FETCH-LOGICAL-c526t-20cb171cdd968b328b67ab431b7c95c2ed5a6484e28c0b35a4891c199503a3073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838365/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838365/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24278212$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ulasov, Ilya</contributor><creatorcontrib>Shoae-Hassani, Alireza</creatorcontrib><creatorcontrib>Keyhanvar, Peyman</creatorcontrib><creatorcontrib>Seifalian, Alexander Marcus</creatorcontrib><creatorcontrib>Mortazavi-Tabatabaei, Seyed Abdolreza</creatorcontrib><creatorcontrib>Ghaderi, Narmin</creatorcontrib><creatorcontrib>Issazadeh, Khosro</creatorcontrib><creatorcontrib>Amirmozafari, Nour</creatorcontrib><creatorcontrib>Verdi, Javad</creatorcontrib><title>λ Phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Using phages is a novel field of cancer therapy and phage nanobioparticles (NBPs) such as λ phage could be modified to deliver and express genetic cassettes into eukaryotic cells safely in contrast with animal viruses. Apoptin, a protein from chicken anemia virus (CAV) has the ability to specifically induce apoptosis only in carcinoma cells. We presented a safe method of breast tumor therapy via the apoptin expressing λ NBPs. Here, we constructed a λ ZAP-CMV-apoptin recombinant NBP and investigated the effectiveness of its apoptotic activity on BT-474, MDA-MB-361, SKBR-3, UACC-812 and ZR-75 cell lines that over-expressing her-2 marker. Apoptosis was evaluated via annexin-V fluorescent iso-thiocyanate/propidium iodide staining, flow-cytometric method and TUNEL assay. Transfection with NBPs carrying λ ZAP-CMV-apoptin significantly inhibited growth of all the breast carcinoma cell lines in vitro. Also nude mice model implanted BT-474 human breast tumor was successfully responded to the systemic and local injection of untargeted recombinant λ NBPs. The results presented here reveal important features of recombinant λ nanobioparticles to serve as safe delivery and expression platform for human cancer therapy.</description><subject>Anemia</subject><subject>Animals</subject><subject>Antineoplastic Agents</subject><subject>Apoptosis</subject><subject>Bacteriophage lambda - genetics</subject><subject>Biotechnology</subject><subject>Breast</subject><subject>Breast cancer</subject><subject>Breast carcinoma</subject><subject>Breast Neoplasms - pathology</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Capsid Proteins - genetics</subject><subject>Cassettes</subject><subject>Cell Division - genetics</subject><subject>ErbB-2 protein</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Fluorescence</subject><subject>Humans</subject><subject>Iodides</subject><subject>Kinases</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Nanoparticles</subject><subject>Phages</subject><subject>Propidium iodide</subject><subject>Recombinant Proteins - 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Apoptin, a protein from chicken anemia virus (CAV) has the ability to specifically induce apoptosis only in carcinoma cells. We presented a safe method of breast tumor therapy via the apoptin expressing λ NBPs. Here, we constructed a λ ZAP-CMV-apoptin recombinant NBP and investigated the effectiveness of its apoptotic activity on BT-474, MDA-MB-361, SKBR-3, UACC-812 and ZR-75 cell lines that over-expressing her-2 marker. Apoptosis was evaluated via annexin-V fluorescent iso-thiocyanate/propidium iodide staining, flow-cytometric method and TUNEL assay. Transfection with NBPs carrying λ ZAP-CMV-apoptin significantly inhibited growth of all the breast carcinoma cell lines in vitro. Also nude mice model implanted BT-474 human breast tumor was successfully responded to the systemic and local injection of untargeted recombinant λ NBPs. The results presented here reveal important features of recombinant λ nanobioparticles to serve as safe delivery and expression platform for human cancer therapy.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24278212</pmid><doi>10.1371/journal.pone.0079907</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Anemia Animals Antineoplastic Agents Apoptosis Bacteriophage lambda - genetics Biotechnology Breast Breast cancer Breast carcinoma Breast Neoplasms - pathology Cancer Cancer therapies Capsid Proteins - genetics Cassettes Cell Division - genetics ErbB-2 protein Female Flow Cytometry Fluorescence Humans Iodides Kinases Mice Mice, Nude Nanoparticles Phages Propidium iodide Recombinant Proteins - genetics Science Surgical implants Therapy Transfection Tumor cell lines Viruses |
| title | λ Phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo |
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