Transcriptome analysis of medicinal plant Salvia miltiorrhiza and identification of genes related to tanshinone biosynthesis
Salvia miltiorrhiza Bunge, a perennial plant of Lamiaceae, accumulates abietane-type diterpenoids of tanshinones in root, which have been used as traditional Chinese medicine to treat neuroasthenic insomnia and cardiovascular diseases. However, to date the biosynthetic pathway of tanshinones is only...
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| creator | Yang, Lei Ding, Guohui Lin, Haiyan Cheng, Haining Kong, Yu Wei, Yukun Fang, Xin Liu, Renyi Wang, Lingiian Chen, Xiaoya Yang, Changqing |
| description | Salvia miltiorrhiza Bunge, a perennial plant of Lamiaceae, accumulates abietane-type diterpenoids of tanshinones in root, which have been used as traditional Chinese medicine to treat neuroasthenic insomnia and cardiovascular diseases. However, to date the biosynthetic pathway of tanshinones is only partially elucidated and the mechanism for their root-specific accumulation remains unknown. To identify enzymes and transcriptional regulators involved in the biosynthesis of tanshinones, we conducted transcriptome profiling of S. miltiorrhiza root and leaf tissues using the 454 GS-FLX pyrosequencing platform, which generated 550,546 and 525,292 reads, respectively. RNA sequencing reads were assembled and clustered into 64,139 unigenes (29,883 isotigs and 34,256 singletons). NCBI non-redundant protein databases (NR) and Swiss-Prot database searches anchored 32,096 unigenes (50%) with functional annotations based on sequence similarities. Further assignments with Gene Ontology (GO) terms and KEGG biochemical pathways identified 168 unigenes referring to the terpenoid backbone biosynthesis (including 144 MEP and MVA pathway genes and 24 terpene synthases). Comparative analysis of the transcriptomes identified 2,863 unigenes that were highly expressed in roots, including those encoding enzymes of early steps of tanshinone biosynthetic pathway, such as copalyl diphosphate synthase (SmCPS), kaurene synthase-like (SmKSL) and CYP76AH1. Other differentially expressed unigenes predicted to be related to tanshinone biosynthesis fall into cytochrome P450 monooxygenases, dehydrogenases and reductases, as well as regulatory factors. In addition, 21 P450 genes were selectively confirmed by real-time PCR. Thus we have generated a large unigene dataset which provides a valuable resource for further investigation of the radix development and biosynthesis of tanshinones. |
| doi_str_mv | 10.1371/journal.pone.0080464 |
| format | Article |
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However, to date the biosynthetic pathway of tanshinones is only partially elucidated and the mechanism for their root-specific accumulation remains unknown. To identify enzymes and transcriptional regulators involved in the biosynthesis of tanshinones, we conducted transcriptome profiling of S. miltiorrhiza root and leaf tissues using the 454 GS-FLX pyrosequencing platform, which generated 550,546 and 525,292 reads, respectively. RNA sequencing reads were assembled and clustered into 64,139 unigenes (29,883 isotigs and 34,256 singletons). NCBI non-redundant protein databases (NR) and Swiss-Prot database searches anchored 32,096 unigenes (50%) with functional annotations based on sequence similarities. Further assignments with Gene Ontology (GO) terms and KEGG biochemical pathways identified 168 unigenes referring to the terpenoid backbone biosynthesis (including 144 MEP and MVA pathway genes and 24 terpene synthases). Comparative analysis of the transcriptomes identified 2,863 unigenes that were highly expressed in roots, including those encoding enzymes of early steps of tanshinone biosynthetic pathway, such as copalyl diphosphate synthase (SmCPS), kaurene synthase-like (SmKSL) and CYP76AH1. Other differentially expressed unigenes predicted to be related to tanshinone biosynthesis fall into cytochrome P450 monooxygenases, dehydrogenases and reductases, as well as regulatory factors. In addition, 21 P450 genes were selectively confirmed by real-time PCR. Thus we have generated a large unigene dataset which provides a valuable resource for further investigation of the radix development and biosynthesis of tanshinones.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0080464</identifier><identifier>PMID: 24260395</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Alkyl and Aryl Transferases - metabolism ; Analysis ; Annotations ; Bioinformatics ; Biology ; Biosynthesis ; Biosynthetic Pathways ; Cardiovascular diseases ; Cell cycle ; Cell division ; Comparative analysis ; Computational Biology - methods ; Computer programs ; Cytochrome ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P450 ; Diterpenes ; Diterpenes, Abietane - biosynthesis ; Enzymes ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Gene sequencing ; Genes ; Genomes ; Genomics ; Heart diseases ; Herbal medicine ; High-Throughput Nucleotide Sequencing ; Insomnia ; Kinases ; Laboratories ; Medicinal plants ; Mevalonate pathway ; Molecular Sequence Data ; Natural products ; Online searching ; Oxidoreductases ; Phylogeny ; Physiological aspects ; Physiology ; Plant Leaves - genetics ; Plant Leaves - metabolism ; Plant Roots - genetics ; Plant Roots - metabolism ; Plant sciences ; Plant tissues ; Plants, Medicinal - genetics ; Plants, Medicinal - metabolism ; Reductases ; Regulators ; Ribonucleic acid ; RNA ; RNA sequencing ; Salvia miltiorrhiza ; Salvia miltiorrhiza - classification ; Salvia miltiorrhiza - genetics ; Salvia miltiorrhiza - metabolism ; Sleep disorders ; Tanshinones ; Terpenes - metabolism ; Traditional Chinese medicine ; Transcription ; Transcription factors ; Transcription Factors - metabolism ; Transcriptome</subject><ispartof>PloS one, 2013-11, Vol.8 (11), p.e80464-e80464</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Yang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/3.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Yang et al 2013 Yang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-30ce6f0659d7933fcbe438bd299b41186da0239d7d011850fce7d3041a5435ce3</citedby><cites>FETCH-LOGICAL-c692t-30ce6f0659d7933fcbe438bd299b41186da0239d7d011850fce7d3041a5435ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834075/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834075/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24260395$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Neilan, Brett</contributor><creatorcontrib>Yang, Lei</creatorcontrib><creatorcontrib>Ding, Guohui</creatorcontrib><creatorcontrib>Lin, Haiyan</creatorcontrib><creatorcontrib>Cheng, Haining</creatorcontrib><creatorcontrib>Kong, Yu</creatorcontrib><creatorcontrib>Wei, Yukun</creatorcontrib><creatorcontrib>Fang, Xin</creatorcontrib><creatorcontrib>Liu, Renyi</creatorcontrib><creatorcontrib>Wang, Lingiian</creatorcontrib><creatorcontrib>Chen, Xiaoya</creatorcontrib><creatorcontrib>Yang, Changqing</creatorcontrib><title>Transcriptome analysis of medicinal plant Salvia miltiorrhiza and identification of genes related to tanshinone biosynthesis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Salvia miltiorrhiza Bunge, a perennial plant of Lamiaceae, accumulates abietane-type diterpenoids of tanshinones in root, which have been used as traditional Chinese medicine to treat neuroasthenic insomnia and cardiovascular diseases. However, to date the biosynthetic pathway of tanshinones is only partially elucidated and the mechanism for their root-specific accumulation remains unknown. To identify enzymes and transcriptional regulators involved in the biosynthesis of tanshinones, we conducted transcriptome profiling of S. miltiorrhiza root and leaf tissues using the 454 GS-FLX pyrosequencing platform, which generated 550,546 and 525,292 reads, respectively. RNA sequencing reads were assembled and clustered into 64,139 unigenes (29,883 isotigs and 34,256 singletons). NCBI non-redundant protein databases (NR) and Swiss-Prot database searches anchored 32,096 unigenes (50%) with functional annotations based on sequence similarities. Further assignments with Gene Ontology (GO) terms and KEGG biochemical pathways identified 168 unigenes referring to the terpenoid backbone biosynthesis (including 144 MEP and MVA pathway genes and 24 terpene synthases). Comparative analysis of the transcriptomes identified 2,863 unigenes that were highly expressed in roots, including those encoding enzymes of early steps of tanshinone biosynthetic pathway, such as copalyl diphosphate synthase (SmCPS), kaurene synthase-like (SmKSL) and CYP76AH1. Other differentially expressed unigenes predicted to be related to tanshinone biosynthesis fall into cytochrome P450 monooxygenases, dehydrogenases and reductases, as well as regulatory factors. In addition, 21 P450 genes were selectively confirmed by real-time PCR. Thus we have generated a large unigene dataset which provides a valuable resource for further investigation of the radix development and biosynthesis of tanshinones.</description><subject>Acids</subject><subject>Alkyl and Aryl Transferases - metabolism</subject><subject>Analysis</subject><subject>Annotations</subject><subject>Bioinformatics</subject><subject>Biology</subject><subject>Biosynthesis</subject><subject>Biosynthetic Pathways</subject><subject>Cardiovascular diseases</subject><subject>Cell cycle</subject><subject>Cell division</subject><subject>Comparative analysis</subject><subject>Computational Biology - methods</subject><subject>Computer programs</subject><subject>Cytochrome</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochrome P450</subject><subject>Diterpenes</subject><subject>Diterpenes, Abietane - biosynthesis</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Heart diseases</subject><subject>Herbal medicine</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Insomnia</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Medicinal plants</subject><subject>Mevalonate pathway</subject><subject>Molecular Sequence Data</subject><subject>Natural products</subject><subject>Online searching</subject><subject>Oxidoreductases</subject><subject>Phylogeny</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Plant Leaves - genetics</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Roots - genetics</subject><subject>Plant Roots - metabolism</subject><subject>Plant sciences</subject><subject>Plant tissues</subject><subject>Plants, Medicinal - genetics</subject><subject>Plants, Medicinal - metabolism</subject><subject>Reductases</subject><subject>Regulators</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA sequencing</subject><subject>Salvia miltiorrhiza</subject><subject>Salvia miltiorrhiza - classification</subject><subject>Salvia miltiorrhiza - genetics</subject><subject>Salvia miltiorrhiza - metabolism</subject><subject>Sleep disorders</subject><subject>Tanshinones</subject><subject>Terpenes - metabolism</subject><subject>Traditional Chinese medicine</subject><subject>Transcription</subject><subject>Transcription factors</subject><subject>Transcription Factors - metabolism</subject><subject>Transcriptome</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk01v1DAQhiMEoqXwDxBEQkJw2MWOna8LUlXxsVKlSrRwtRx7svHKG29tp6KIH8-ETasN6gHlkHj8zPvaM5kkeUnJkrKSfti4wffSLneuhyUhFeEFf5Qc05pliyIj7PHB91HyLIQNITmriuJpcpTxrCCszo-T31de9kF5s4tuC6lExdtgQuradAvaKIOBdGdlH9NLaW-MTLfGRuO878wvibxOjYY-mtYoifF-zFxDDyH1YGUEnUaXRvToTI8nTRvjwm0fO0CX58mTVtoAL6b3SfL986ers6-L84svq7PT84Uq6iwuGFFQtKTIa13WjLWqAc6qRmd13XBKq0JLkjHc1ARXOWkVlJoRTmXOWa6AnSSv97o764KYChcE5XldVIQyhsRqT2gnN2LnzVb6W-GkEX8Dzq-F9NEoC4JVZdNqqFjelryCWlJaVrItSy451WR0-zi5DQ3WUGF1vLQz0flObzqxdjeozDgpcxR4Nwl4dz1AiGJrggKLXQA3jOcuaIXe2Yi--Qd9-HYTtZZ4AdO3Dn3VKCpOeVllpOR1jdTyAQofDVujsHetwfgs4f0sAZkIP-NaDiGI1eW3_2cvfszZtwdsB9LGLjg7jL9XmIN8DyrvQvDQ3heZEjEOyV01xDgkYhoSTHt12KD7pLupYH8AyLIOrQ</recordid><startdate>20131119</startdate><enddate>20131119</enddate><creator>Yang, Lei</creator><creator>Ding, Guohui</creator><creator>Lin, Haiyan</creator><creator>Cheng, Haining</creator><creator>Kong, Yu</creator><creator>Wei, Yukun</creator><creator>Fang, Xin</creator><creator>Liu, Renyi</creator><creator>Wang, Lingiian</creator><creator>Chen, Xiaoya</creator><creator>Yang, Changqing</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131119</creationdate><title>Transcriptome analysis of medicinal plant Salvia miltiorrhiza and identification of genes related to tanshinone biosynthesis</title><author>Yang, Lei ; Ding, Guohui ; Lin, Haiyan ; Cheng, Haining ; Kong, Yu ; Wei, Yukun ; Fang, Xin ; Liu, Renyi ; Wang, Lingiian ; Chen, Xiaoya ; Yang, Changqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-30ce6f0659d7933fcbe438bd299b41186da0239d7d011850fce7d3041a5435ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acids</topic><topic>Alkyl and Aryl Transferases - metabolism</topic><topic>Analysis</topic><topic>Annotations</topic><topic>Bioinformatics</topic><topic>Biology</topic><topic>Biosynthesis</topic><topic>Biosynthetic Pathways</topic><topic>Cardiovascular diseases</topic><topic>Cell cycle</topic><topic>Cell division</topic><topic>Comparative analysis</topic><topic>Computational Biology - methods</topic><topic>Computer programs</topic><topic>Cytochrome</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>Cytochrome P450</topic><topic>Diterpenes</topic><topic>Diterpenes, Abietane - biosynthesis</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Heart diseases</topic><topic>Herbal medicine</topic><topic>High-Throughput Nucleotide Sequencing</topic><topic>Insomnia</topic><topic>Kinases</topic><topic>Laboratories</topic><topic>Medicinal plants</topic><topic>Mevalonate pathway</topic><topic>Molecular Sequence Data</topic><topic>Natural products</topic><topic>Online searching</topic><topic>Oxidoreductases</topic><topic>Phylogeny</topic><topic>Physiological aspects</topic><topic>Physiology</topic><topic>Plant Leaves - 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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Lei</au><au>Ding, Guohui</au><au>Lin, Haiyan</au><au>Cheng, Haining</au><au>Kong, Yu</au><au>Wei, Yukun</au><au>Fang, Xin</au><au>Liu, Renyi</au><au>Wang, Lingiian</au><au>Chen, Xiaoya</au><au>Yang, Changqing</au><au>Neilan, Brett</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptome analysis of medicinal plant Salvia miltiorrhiza and identification of genes related to tanshinone biosynthesis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-11-19</date><risdate>2013</risdate><volume>8</volume><issue>11</issue><spage>e80464</spage><epage>e80464</epage><pages>e80464-e80464</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Salvia miltiorrhiza Bunge, a perennial plant of Lamiaceae, accumulates abietane-type diterpenoids of tanshinones in root, which have been used as traditional Chinese medicine to treat neuroasthenic insomnia and cardiovascular diseases. However, to date the biosynthetic pathway of tanshinones is only partially elucidated and the mechanism for their root-specific accumulation remains unknown. To identify enzymes and transcriptional regulators involved in the biosynthesis of tanshinones, we conducted transcriptome profiling of S. miltiorrhiza root and leaf tissues using the 454 GS-FLX pyrosequencing platform, which generated 550,546 and 525,292 reads, respectively. RNA sequencing reads were assembled and clustered into 64,139 unigenes (29,883 isotigs and 34,256 singletons). NCBI non-redundant protein databases (NR) and Swiss-Prot database searches anchored 32,096 unigenes (50%) with functional annotations based on sequence similarities. Further assignments with Gene Ontology (GO) terms and KEGG biochemical pathways identified 168 unigenes referring to the terpenoid backbone biosynthesis (including 144 MEP and MVA pathway genes and 24 terpene synthases). Comparative analysis of the transcriptomes identified 2,863 unigenes that were highly expressed in roots, including those encoding enzymes of early steps of tanshinone biosynthetic pathway, such as copalyl diphosphate synthase (SmCPS), kaurene synthase-like (SmKSL) and CYP76AH1. Other differentially expressed unigenes predicted to be related to tanshinone biosynthesis fall into cytochrome P450 monooxygenases, dehydrogenases and reductases, as well as regulatory factors. In addition, 21 P450 genes were selectively confirmed by real-time PCR. Thus we have generated a large unigene dataset which provides a valuable resource for further investigation of the radix development and biosynthesis of tanshinones.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24260395</pmid><doi>10.1371/journal.pone.0080464</doi><tpages>e80464</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-11, Vol.8 (11), p.e80464-e80464 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1459680133 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Alkyl and Aryl Transferases - metabolism Analysis Annotations Bioinformatics Biology Biosynthesis Biosynthetic Pathways Cardiovascular diseases Cell cycle Cell division Comparative analysis Computational Biology - methods Computer programs Cytochrome Cytochrome P-450 Enzyme System - genetics Cytochrome P450 Diterpenes Diterpenes, Abietane - biosynthesis Enzymes Gene expression Gene Expression Profiling Gene Expression Regulation, Plant Gene sequencing Genes Genomes Genomics Heart diseases Herbal medicine High-Throughput Nucleotide Sequencing Insomnia Kinases Laboratories Medicinal plants Mevalonate pathway Molecular Sequence Data Natural products Online searching Oxidoreductases Phylogeny Physiological aspects Physiology Plant Leaves - genetics Plant Leaves - metabolism Plant Roots - genetics Plant Roots - metabolism Plant sciences Plant tissues Plants, Medicinal - genetics Plants, Medicinal - metabolism Reductases Regulators Ribonucleic acid RNA RNA sequencing Salvia miltiorrhiza Salvia miltiorrhiza - classification Salvia miltiorrhiza - genetics Salvia miltiorrhiza - metabolism Sleep disorders Tanshinones Terpenes - metabolism Traditional Chinese medicine Transcription Transcription factors Transcription Factors - metabolism Transcriptome |
| title | Transcriptome analysis of medicinal plant Salvia miltiorrhiza and identification of genes related to tanshinone biosynthesis |
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