Protective efficacy of a human endogenous retrovirus envelope-coated, nonreplicable, baculovirus-based hemagglutin vaccine against pandemic influenza H1N1 2009
Despite the advantages of DNA vaccines, overcoming their lower efficacy relative to that of conventional vaccines remains a challenge. Here, we constructed a human endogenous retrovirus (HERV) envelope-coated, nonreplicable, baculovirus-based HA vaccine against swine influenza A/California/04/2009(H...
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| Veröffentlicht in: | PloS one 2013-11, Vol.8 (11), p.e80762-e80762 |
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| creator | Choi, Jae-Yoo Gwon, Yong-Dae Kim, Jeong-Ki Cho, Yeon-Dong Heo, Yoon-Ki Cho, Han-Sam Choi, Tae-Jin Poo, Ha-Ryoung Oh, Yu-Kyoung Kim, Young Bong |
| description | Despite the advantages of DNA vaccines, overcoming their lower efficacy relative to that of conventional vaccines remains a challenge. Here, we constructed a human endogenous retrovirus (HERV) envelope-coated, nonreplicable, baculovirus-based HA vaccine against swine influenza A/California/04/2009(H1N1) hemagglutin (HA) (AcHERV-sH1N1-HA) as an alternative to conventional vaccines and evaluated its efficacy in two strains of mice, BALB/c and C57BL/6. A commercially available, killed virus vaccine was used as a positive control. Mice were intramuscularly administered AcHERV-sH1N1-HA or the commercial vaccine and subsequently given two booster injections. Compared with the commercial vaccine, AcHERV-sH1N1-HA induced significantly higher levels of cellular immune responses in both BALB/c and C57BL/6 mice. Unlike cellular immune responses, humoral immune responses depended on the strain of mice. Following immunization with AcHERV-sH1N1-HA, C57BL/6 mice showed HA-specific IgG titers 10- to 100-fold lower than those of BALB/c mice. In line with the different levels of humoral immune responses, the survival of immunized mice after intranasal challenge with sH1N1 virus (A/California/04/2009) depended on the strain. After challenge with 10-times the median lethal dose (MLD50) of sH1N1 virus, 100% of BALB/c mice immunized with the commercial vaccine or AcHERV-sH1N1-HA survived. In contrast, C57BL/6 mice immunized with AcHERV-sH1N1-HA or the commercial vaccine showed 60% and 70% survival respectively, after challenge with sH1N1 virus. In all mice, virus titers and results of histological analyses of lung tissues were consistent with the survival data. Our results indicate the importance of humoral immune response as a major defense system against influenza viral infection. Moreover, the complete survival of BALB/c mice immunized with AcHERV-sH1N1-HA after challenge with sH1N1 virus suggests the potential of baculoviral vector-based vaccines to achieve an efficacy comparable to that of killed virus vaccines. |
| doi_str_mv | 10.1371/journal.pone.0080762 |
| format | Article |
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Here, we constructed a human endogenous retrovirus (HERV) envelope-coated, nonreplicable, baculovirus-based HA vaccine against swine influenza A/California/04/2009(H1N1) hemagglutin (HA) (AcHERV-sH1N1-HA) as an alternative to conventional vaccines and evaluated its efficacy in two strains of mice, BALB/c and C57BL/6. A commercially available, killed virus vaccine was used as a positive control. Mice were intramuscularly administered AcHERV-sH1N1-HA or the commercial vaccine and subsequently given two booster injections. Compared with the commercial vaccine, AcHERV-sH1N1-HA induced significantly higher levels of cellular immune responses in both BALB/c and C57BL/6 mice. Unlike cellular immune responses, humoral immune responses depended on the strain of mice. Following immunization with AcHERV-sH1N1-HA, C57BL/6 mice showed HA-specific IgG titers 10- to 100-fold lower than those of BALB/c mice. In line with the different levels of humoral immune responses, the survival of immunized mice after intranasal challenge with sH1N1 virus (A/California/04/2009) depended on the strain. After challenge with 10-times the median lethal dose (MLD50) of sH1N1 virus, 100% of BALB/c mice immunized with the commercial vaccine or AcHERV-sH1N1-HA survived. In contrast, C57BL/6 mice immunized with AcHERV-sH1N1-HA or the commercial vaccine showed 60% and 70% survival respectively, after challenge with sH1N1 virus. In all mice, virus titers and results of histological analyses of lung tissues were consistent with the survival data. Our results indicate the importance of humoral immune response as a major defense system against influenza viral infection. Moreover, the complete survival of BALB/c mice immunized with AcHERV-sH1N1-HA after challenge with sH1N1 virus suggests the potential of baculoviral vector-based vaccines to achieve an efficacy comparable to that of killed virus vaccines.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0080762</identifier><identifier>PMID: 24260476</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animal tissues ; Animals ; Avian flu ; Baculoviridae - genetics ; Baculoviridae - immunology ; Baculovirus ; Biotechnology ; Cell Line ; Dendritic cells ; Deoxyribonucleic acid ; DNA ; DNA vaccines ; Drug therapy ; Effectiveness ; Eggs ; Endogenous Retroviruses - genetics ; Female ; Gene Expression ; Gene Order ; Genes ; Genetic Vectors - administration & dosage ; Genetic Vectors - genetics ; Genetic Vectors - immunology ; Health aspects ; Hemagglutinin Glycoproteins, Influenza Virus - genetics ; Hemagglutinin Glycoproteins, Influenza Virus - immunology ; House mouse ; Humans ; Immune response ; Immune response (cell-mediated) ; Immune response (humoral) ; Immune system ; Immunity, Cellular ; Immunity, Humoral ; Immunization ; Immunoglobulin G ; Influenza ; Influenza A ; Influenza A Virus, H1N1 Subtype - immunology ; Influenza vaccines ; Influenza Vaccines - administration & dosage ; Influenza Vaccines - genetics ; Influenza Vaccines - immunology ; Laboratory animals ; Lethal dose ; Livestock ; Lung - pathology ; Lung - virology ; Lungs ; Mice ; Orthomyxoviridae Infections - mortality ; Orthomyxoviridae Infections - prevention & control ; Pandemics ; Pharmacy ; Proteomics ; Survival ; Swine ; Swine flu ; Swine influenza ; Vaccines ; Viral Envelope Proteins - genetics ; Viruses</subject><ispartof>PloS one, 2013-11, Vol.8 (11), p.e80762-e80762</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Choi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/3.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Choi et al 2013 Choi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-a6ab80bdfb4974135ea67ee9f25308b6822e7103d4af1345ef730cd570b18bfa3</citedby><cites>FETCH-LOGICAL-c692t-a6ab80bdfb4974135ea67ee9f25308b6822e7103d4af1345ef730cd570b18bfa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3832454/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3832454/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2101,2927,23865,27923,27924,53790,53792,79471,79472</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24260476$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kobinger, Gary P</contributor><creatorcontrib>Choi, Jae-Yoo</creatorcontrib><creatorcontrib>Gwon, Yong-Dae</creatorcontrib><creatorcontrib>Kim, Jeong-Ki</creatorcontrib><creatorcontrib>Cho, Yeon-Dong</creatorcontrib><creatorcontrib>Heo, Yoon-Ki</creatorcontrib><creatorcontrib>Cho, Han-Sam</creatorcontrib><creatorcontrib>Choi, Tae-Jin</creatorcontrib><creatorcontrib>Poo, Ha-Ryoung</creatorcontrib><creatorcontrib>Oh, Yu-Kyoung</creatorcontrib><creatorcontrib>Kim, Young Bong</creatorcontrib><title>Protective efficacy of a human endogenous retrovirus envelope-coated, nonreplicable, baculovirus-based hemagglutin vaccine against pandemic influenza H1N1 2009</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Despite the advantages of DNA vaccines, overcoming their lower efficacy relative to that of conventional vaccines remains a challenge. Here, we constructed a human endogenous retrovirus (HERV) envelope-coated, nonreplicable, baculovirus-based HA vaccine against swine influenza A/California/04/2009(H1N1) hemagglutin (HA) (AcHERV-sH1N1-HA) as an alternative to conventional vaccines and evaluated its efficacy in two strains of mice, BALB/c and C57BL/6. A commercially available, killed virus vaccine was used as a positive control. Mice were intramuscularly administered AcHERV-sH1N1-HA or the commercial vaccine and subsequently given two booster injections. Compared with the commercial vaccine, AcHERV-sH1N1-HA induced significantly higher levels of cellular immune responses in both BALB/c and C57BL/6 mice. Unlike cellular immune responses, humoral immune responses depended on the strain of mice. Following immunization with AcHERV-sH1N1-HA, C57BL/6 mice showed HA-specific IgG titers 10- to 100-fold lower than those of BALB/c mice. In line with the different levels of humoral immune responses, the survival of immunized mice after intranasal challenge with sH1N1 virus (A/California/04/2009) depended on the strain. After challenge with 10-times the median lethal dose (MLD50) of sH1N1 virus, 100% of BALB/c mice immunized with the commercial vaccine or AcHERV-sH1N1-HA survived. In contrast, C57BL/6 mice immunized with AcHERV-sH1N1-HA or the commercial vaccine showed 60% and 70% survival respectively, after challenge with sH1N1 virus. In all mice, virus titers and results of histological analyses of lung tissues were consistent with the survival data. Our results indicate the importance of humoral immune response as a major defense system against influenza viral infection. 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immunology</topic><topic>Influenza vaccines</topic><topic>Influenza Vaccines - administration & dosage</topic><topic>Influenza Vaccines - genetics</topic><topic>Influenza Vaccines - immunology</topic><topic>Laboratory animals</topic><topic>Lethal dose</topic><topic>Livestock</topic><topic>Lung - pathology</topic><topic>Lung - virology</topic><topic>Lungs</topic><topic>Mice</topic><topic>Orthomyxoviridae Infections - mortality</topic><topic>Orthomyxoviridae Infections - prevention & control</topic><topic>Pandemics</topic><topic>Pharmacy</topic><topic>Proteomics</topic><topic>Survival</topic><topic>Swine</topic><topic>Swine flu</topic><topic>Swine influenza</topic><topic>Vaccines</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, Jae-Yoo</creatorcontrib><creatorcontrib>Gwon, Yong-Dae</creatorcontrib><creatorcontrib>Kim, Jeong-Ki</creatorcontrib><creatorcontrib>Cho, Yeon-Dong</creatorcontrib><creatorcontrib>Heo, Yoon-Ki</creatorcontrib><creatorcontrib>Cho, Han-Sam</creatorcontrib><creatorcontrib>Choi, Tae-Jin</creatorcontrib><creatorcontrib>Poo, Ha-Ryoung</creatorcontrib><creatorcontrib>Oh, Yu-Kyoung</creatorcontrib><creatorcontrib>Kim, Young Bong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choi, Jae-Yoo</au><au>Gwon, Yong-Dae</au><au>Kim, Jeong-Ki</au><au>Cho, Yeon-Dong</au><au>Heo, Yoon-Ki</au><au>Cho, Han-Sam</au><au>Choi, Tae-Jin</au><au>Poo, Ha-Ryoung</au><au>Oh, Yu-Kyoung</au><au>Kim, Young Bong</au><au>Kobinger, Gary P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective efficacy of a human endogenous retrovirus envelope-coated, nonreplicable, baculovirus-based hemagglutin vaccine against pandemic influenza H1N1 2009</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-11-18</date><risdate>2013</risdate><volume>8</volume><issue>11</issue><spage>e80762</spage><epage>e80762</epage><pages>e80762-e80762</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Despite the advantages of DNA vaccines, overcoming their lower efficacy relative to that of conventional vaccines remains a challenge. Here, we constructed a human endogenous retrovirus (HERV) envelope-coated, nonreplicable, baculovirus-based HA vaccine against swine influenza A/California/04/2009(H1N1) hemagglutin (HA) (AcHERV-sH1N1-HA) as an alternative to conventional vaccines and evaluated its efficacy in two strains of mice, BALB/c and C57BL/6. A commercially available, killed virus vaccine was used as a positive control. Mice were intramuscularly administered AcHERV-sH1N1-HA or the commercial vaccine and subsequently given two booster injections. Compared with the commercial vaccine, AcHERV-sH1N1-HA induced significantly higher levels of cellular immune responses in both BALB/c and C57BL/6 mice. Unlike cellular immune responses, humoral immune responses depended on the strain of mice. Following immunization with AcHERV-sH1N1-HA, C57BL/6 mice showed HA-specific IgG titers 10- to 100-fold lower than those of BALB/c mice. In line with the different levels of humoral immune responses, the survival of immunized mice after intranasal challenge with sH1N1 virus (A/California/04/2009) depended on the strain. After challenge with 10-times the median lethal dose (MLD50) of sH1N1 virus, 100% of BALB/c mice immunized with the commercial vaccine or AcHERV-sH1N1-HA survived. In contrast, C57BL/6 mice immunized with AcHERV-sH1N1-HA or the commercial vaccine showed 60% and 70% survival respectively, after challenge with sH1N1 virus. In all mice, virus titers and results of histological analyses of lung tissues were consistent with the survival data. Our results indicate the importance of humoral immune response as a major defense system against influenza viral infection. Moreover, the complete survival of BALB/c mice immunized with AcHERV-sH1N1-HA after challenge with sH1N1 virus suggests the potential of baculoviral vector-based vaccines to achieve an efficacy comparable to that of killed virus vaccines.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24260476</pmid><doi>10.1371/journal.pone.0080762</doi><tpages>e80762</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-11, Vol.8 (11), p.e80762-e80762 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1459403966 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animal tissues Animals Avian flu Baculoviridae - genetics Baculoviridae - immunology Baculovirus Biotechnology Cell Line Dendritic cells Deoxyribonucleic acid DNA DNA vaccines Drug therapy Effectiveness Eggs Endogenous Retroviruses - genetics Female Gene Expression Gene Order Genes Genetic Vectors - administration & dosage Genetic Vectors - genetics Genetic Vectors - immunology Health aspects Hemagglutinin Glycoproteins, Influenza Virus - genetics Hemagglutinin Glycoproteins, Influenza Virus - immunology House mouse Humans Immune response Immune response (cell-mediated) Immune response (humoral) Immune system Immunity, Cellular Immunity, Humoral Immunization Immunoglobulin G Influenza Influenza A Influenza A Virus, H1N1 Subtype - immunology Influenza vaccines Influenza Vaccines - administration & dosage Influenza Vaccines - genetics Influenza Vaccines - immunology Laboratory animals Lethal dose Livestock Lung - pathology Lung - virology Lungs Mice Orthomyxoviridae Infections - mortality Orthomyxoviridae Infections - prevention & control Pandemics Pharmacy Proteomics Survival Swine Swine flu Swine influenza Vaccines Viral Envelope Proteins - genetics Viruses |
| title | Protective efficacy of a human endogenous retrovirus envelope-coated, nonreplicable, baculovirus-based hemagglutin vaccine against pandemic influenza H1N1 2009 |
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