Genome-wide expression profiling of complex regional pain syndrome
Complex regional pain syndrome (CRPS) is a chronic, progressive, and devastating pain syndrome characterized by spontaneous pain, hyperalgesia, allodynia, altered skin temperature, and motor dysfunction. Although previous gene expression profiling studies have been conducted in animal pain models, t...
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| Veröffentlicht in: | PloS one 2013-11, Vol.8 (11), p.e79435-e79435 |
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| description | Complex regional pain syndrome (CRPS) is a chronic, progressive, and devastating pain syndrome characterized by spontaneous pain, hyperalgesia, allodynia, altered skin temperature, and motor dysfunction. Although previous gene expression profiling studies have been conducted in animal pain models, there genome-wide expression profiling in the whole blood of CRPS patients has not been reported yet. Here, we successfully identified certain pain-related genes through genome-wide expression profiling in the blood from CRPS patients. We found that 80 genes were differentially expressed between 4 CRPS patients (2 CRPS I and 2 CRPS II) and 5 controls (cut-off value: 1.5-fold change and p |
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Although previous gene expression profiling studies have been conducted in animal pain models, there genome-wide expression profiling in the whole blood of CRPS patients has not been reported yet. Here, we successfully identified certain pain-related genes through genome-wide expression profiling in the blood from CRPS patients. We found that 80 genes were differentially expressed between 4 CRPS patients (2 CRPS I and 2 CRPS II) and 5 controls (cut-off value: 1.5-fold change and p<0.05). Most of those genes were associated with signal transduction, developmental processes, cell structure and motility, and immunity and defense. The expression levels of major histocompatibility complex class I A subtype (HLA-A29.1), matrix metalloproteinase 9 (MMP9), alanine aminopeptidase N (ANPEP), l-histidine decarboxylase (HDC), granulocyte colony-stimulating factor 3 receptor (G-CSF3R), and signal transducer and activator of transcription 3 (STAT3) genes selected from the microarray were confirmed in 24 CRPS patients and 18 controls by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). We focused on the MMP9 gene that, by qRT-PCR, showed a statistically significant difference in expression in CRPS patients compared to controls with the highest relative fold change (4.0±1.23 times and p = 1.4×10(-4)). The up-regulation of MMP9 gene in the blood may be related to the pain progression in CRPS patients. Our findings, which offer a valuable contribution to the understanding of the differential gene expression in CRPS may help in the understanding of the pathophysiology of CRPS pain progression.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0079435</identifier><identifier>PMID: 24244504</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Alanine ; Aminopeptidase ; Analysis ; Anesthesiology ; Animal models ; Antigens ; Blood ; Brain research ; Cellular signal transduction ; Clinical trials ; Cluster Analysis ; Colony-stimulating factor ; Complex regional pain syndrome ; Complex Regional Pain Syndromes - genetics ; Complex Regional Pain Syndromes - physiopathology ; Cytokines ; Cytology ; DNA microarrays ; Female ; Gelatinase B ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation ; Genes ; Genome-Wide Association Study ; Genomes ; Genomics ; Granulocyte colony-stimulating factor ; Growth factors ; Histidine ; Histidine decarboxylase ; Histocompatibility antigen HLA ; Hospitals ; Humans ; Hyperalgesia ; Immunity ; Leukocytes (granulocytic) ; Major histocompatibility complex ; Male ; Matrix metalloproteinase ; Medicine ; Metalloproteinase ; Middle Aged ; Molecular Sequence Annotation ; Pain ; Pain perception ; Pathogenesis ; Patients ; Polymerase chain reaction ; Reproducibility of Results ; Reverse transcription ; Rodents ; Signal processing ; Skin ; Skin temperature ; Stat3 protein ; Statistical analysis ; Transcription (Genetics) ; Tumor necrosis factor-TNF ; Young Adult</subject><ispartof>PloS one, 2013-11, Vol.8 (11), p.e79435-e79435</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Jin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Jin et al 2013 Jin et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c784t-38ec707b69f226707170046f2ed453367d67f5d6d0006bb2262ce9008dd598a3</citedby><cites>FETCH-LOGICAL-c784t-38ec707b69f226707170046f2ed453367d67f5d6d0006bb2262ce9008dd598a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828360/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828360/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24244504$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Costigan, Michael</contributor><creatorcontrib>Jin, Eun-Heui</creatorcontrib><creatorcontrib>Zhang, Enji</creatorcontrib><creatorcontrib>Ko, Youngkwon</creatorcontrib><creatorcontrib>Sim, Woo Seog</creatorcontrib><creatorcontrib>Moon, Dong Eon</creatorcontrib><creatorcontrib>Yoon, Keon Jung</creatorcontrib><creatorcontrib>Hong, Jang Hee</creatorcontrib><creatorcontrib>Lee, Won Hyung</creatorcontrib><title>Genome-wide expression profiling of complex regional pain syndrome</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Complex regional pain syndrome (CRPS) is a chronic, progressive, and devastating pain syndrome characterized by spontaneous pain, hyperalgesia, allodynia, altered skin temperature, and motor dysfunction. Although previous gene expression profiling studies have been conducted in animal pain models, there genome-wide expression profiling in the whole blood of CRPS patients has not been reported yet. Here, we successfully identified certain pain-related genes through genome-wide expression profiling in the blood from CRPS patients. We found that 80 genes were differentially expressed between 4 CRPS patients (2 CRPS I and 2 CRPS II) and 5 controls (cut-off value: 1.5-fold change and p<0.05). Most of those genes were associated with signal transduction, developmental processes, cell structure and motility, and immunity and defense. The expression levels of major histocompatibility complex class I A subtype (HLA-A29.1), matrix metalloproteinase 9 (MMP9), alanine aminopeptidase N (ANPEP), l-histidine decarboxylase (HDC), granulocyte colony-stimulating factor 3 receptor (G-CSF3R), and signal transducer and activator of transcription 3 (STAT3) genes selected from the microarray were confirmed in 24 CRPS patients and 18 controls by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). We focused on the MMP9 gene that, by qRT-PCR, showed a statistically significant difference in expression in CRPS patients compared to controls with the highest relative fold change (4.0±1.23 times and p = 1.4×10(-4)). The up-regulation of MMP9 gene in the blood may be related to the pain progression in CRPS patients. Our findings, which offer a valuable contribution to the understanding of the differential gene expression in CRPS may help in the understanding of the pathophysiology of CRPS pain progression.</description><subject>Adult</subject><subject>Alanine</subject><subject>Aminopeptidase</subject><subject>Analysis</subject><subject>Anesthesiology</subject><subject>Animal models</subject><subject>Antigens</subject><subject>Blood</subject><subject>Brain research</subject><subject>Cellular signal transduction</subject><subject>Clinical trials</subject><subject>Cluster Analysis</subject><subject>Colony-stimulating factor</subject><subject>Complex regional pain syndrome</subject><subject>Complex Regional Pain Syndromes - genetics</subject><subject>Complex Regional Pain Syndromes - physiopathology</subject><subject>Cytokines</subject><subject>Cytology</subject><subject>DNA microarrays</subject><subject>Female</subject><subject>Gelatinase B</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Genes</subject><subject>Genome-Wide Association Study</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Granulocyte colony-stimulating factor</subject><subject>Growth factors</subject><subject>Histidine</subject><subject>Histidine decarboxylase</subject><subject>Histocompatibility antigen HLA</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Hyperalgesia</subject><subject>Immunity</subject><subject>Leukocytes (granulocytic)</subject><subject>Major histocompatibility complex</subject><subject>Male</subject><subject>Matrix metalloproteinase</subject><subject>Medicine</subject><subject>Metalloproteinase</subject><subject>Middle Aged</subject><subject>Molecular Sequence Annotation</subject><subject>Pain</subject><subject>Pain perception</subject><subject>Pathogenesis</subject><subject>Patients</subject><subject>Polymerase chain reaction</subject><subject>Reproducibility of Results</subject><subject>Reverse transcription</subject><subject>Rodents</subject><subject>Signal processing</subject><subject>Skin</subject><subject>Skin temperature</subject><subject>Stat3 protein</subject><subject>Statistical analysis</subject><subject>Transcription (Genetics)</subject><subject>Tumor necrosis factor-TNF</subject><subject>Young 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expression profiling of complex regional pain syndrome</title><author>Jin, Eun-Heui ; Zhang, Enji ; Ko, Youngkwon ; Sim, Woo Seog ; Moon, Dong Eon ; Yoon, Keon Jung ; Hong, Jang Hee ; Lee, Won Hyung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c784t-38ec707b69f226707170046f2ed453367d67f5d6d0006bb2262ce9008dd598a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Alanine</topic><topic>Aminopeptidase</topic><topic>Analysis</topic><topic>Anesthesiology</topic><topic>Animal models</topic><topic>Antigens</topic><topic>Blood</topic><topic>Brain research</topic><topic>Cellular signal transduction</topic><topic>Clinical trials</topic><topic>Cluster Analysis</topic><topic>Colony-stimulating factor</topic><topic>Complex regional pain syndrome</topic><topic>Complex Regional Pain Syndromes - genetics</topic><topic>Complex Regional Pain Syndromes - physiopathology</topic><topic>Cytokines</topic><topic>Cytology</topic><topic>DNA microarrays</topic><topic>Female</topic><topic>Gelatinase B</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Genes</topic><topic>Genome-Wide Association Study</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Granulocyte colony-stimulating factor</topic><topic>Growth factors</topic><topic>Histidine</topic><topic>Histidine decarboxylase</topic><topic>Histocompatibility antigen HLA</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Hyperalgesia</topic><topic>Immunity</topic><topic>Leukocytes (granulocytic)</topic><topic>Major histocompatibility complex</topic><topic>Male</topic><topic>Matrix metalloproteinase</topic><topic>Medicine</topic><topic>Metalloproteinase</topic><topic>Middle Aged</topic><topic>Molecular Sequence Annotation</topic><topic>Pain</topic><topic>Pain perception</topic><topic>Pathogenesis</topic><topic>Patients</topic><topic>Polymerase chain reaction</topic><topic>Reproducibility of Results</topic><topic>Reverse transcription</topic><topic>Rodents</topic><topic>Signal processing</topic><topic>Skin</topic><topic>Skin temperature</topic><topic>Stat3 protein</topic><topic>Statistical analysis</topic><topic>Transcription (Genetics)</topic><topic>Tumor necrosis factor-TNF</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jin, Eun-Heui</creatorcontrib><creatorcontrib>Zhang, Enji</creatorcontrib><creatorcontrib>Ko, Youngkwon</creatorcontrib><creatorcontrib>Sim, Woo Seog</creatorcontrib><creatorcontrib>Moon, Dong Eon</creatorcontrib><creatorcontrib>Yoon, Keon Jung</creatorcontrib><creatorcontrib>Hong, Jang Hee</creatorcontrib><creatorcontrib>Lee, Won Hyung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE 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expression profiling of complex regional pain syndrome</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-11-14</date><risdate>2013</risdate><volume>8</volume><issue>11</issue><spage>e79435</spage><epage>e79435</epage><pages>e79435-e79435</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Complex regional pain syndrome (CRPS) is a chronic, progressive, and devastating pain syndrome characterized by spontaneous pain, hyperalgesia, allodynia, altered skin temperature, and motor dysfunction. Although previous gene expression profiling studies have been conducted in animal pain models, there genome-wide expression profiling in the whole blood of CRPS patients has not been reported yet. Here, we successfully identified certain pain-related genes through genome-wide expression profiling in the blood from CRPS patients. We found that 80 genes were differentially expressed between 4 CRPS patients (2 CRPS I and 2 CRPS II) and 5 controls (cut-off value: 1.5-fold change and p<0.05). Most of those genes were associated with signal transduction, developmental processes, cell structure and motility, and immunity and defense. The expression levels of major histocompatibility complex class I A subtype (HLA-A29.1), matrix metalloproteinase 9 (MMP9), alanine aminopeptidase N (ANPEP), l-histidine decarboxylase (HDC), granulocyte colony-stimulating factor 3 receptor (G-CSF3R), and signal transducer and activator of transcription 3 (STAT3) genes selected from the microarray were confirmed in 24 CRPS patients and 18 controls by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). We focused on the MMP9 gene that, by qRT-PCR, showed a statistically significant difference in expression in CRPS patients compared to controls with the highest relative fold change (4.0±1.23 times and p = 1.4×10(-4)). The up-regulation of MMP9 gene in the blood may be related to the pain progression in CRPS patients. Our findings, which offer a valuable contribution to the understanding of the differential gene expression in CRPS may help in the understanding of the pathophysiology of CRPS pain progression.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24244504</pmid><doi>10.1371/journal.pone.0079435</doi><tpages>e79435</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Alanine Aminopeptidase Analysis Anesthesiology Animal models Antigens Blood Brain research Cellular signal transduction Clinical trials Cluster Analysis Colony-stimulating factor Complex regional pain syndrome Complex Regional Pain Syndromes - genetics Complex Regional Pain Syndromes - physiopathology Cytokines Cytology DNA microarrays Female Gelatinase B Gene expression Gene Expression Profiling Gene Expression Regulation Genes Genome-Wide Association Study Genomes Genomics Granulocyte colony-stimulating factor Growth factors Histidine Histidine decarboxylase Histocompatibility antigen HLA Hospitals Humans Hyperalgesia Immunity Leukocytes (granulocytic) Major histocompatibility complex Male Matrix metalloproteinase Medicine Metalloproteinase Middle Aged Molecular Sequence Annotation Pain Pain perception Pathogenesis Patients Polymerase chain reaction Reproducibility of Results Reverse transcription Rodents Signal processing Skin Skin temperature Stat3 protein Statistical analysis Transcription (Genetics) Tumor necrosis factor-TNF Young Adult |
| title | Genome-wide expression profiling of complex regional pain syndrome |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T02%3A16%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genome-wide%20expression%20profiling%20of%20complex%20regional%20pain%20syndrome&rft.jtitle=PloS%20one&rft.au=Jin,%20Eun-Heui&rft.date=2013-11-14&rft.volume=8&rft.issue=11&rft.spage=e79435&rft.epage=e79435&rft.pages=e79435-e79435&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0079435&rft_dat=%3Cgale_plos_%3EA478276901%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1458577128&rft_id=info:pmid/24244504&rft_galeid=A478276901&rft_doaj_id=oai_doaj_org_article_1baf9ab3990246948929121ac6f0915b&rfr_iscdi=true |