Identification and molecular characterization of FKF1 and GI homologous genes in soybean
In Arabidopsis, FKF1 (FLAVIN BINDING, KELCH REPEAT, F-BOX1) and GI (GIGANTEA) play important roles in flowering pathway through regulating daytime CO (CONSTANS) expression, and such a function is conserved across plants studied. But related reports are limited for soybean. In this study, we cloned F...
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| description | In Arabidopsis, FKF1 (FLAVIN BINDING, KELCH REPEAT, F-BOX1) and GI (GIGANTEA) play important roles in flowering pathway through regulating daytime CO (CONSTANS) expression, and such a function is conserved across plants studied. But related reports are limited for soybean. In this study, we cloned FKF1 and GI homologs in soybean, and named as GmFKF1, GmFKF2, GmGI1, GmGI2, and GmGI3, respectively. GmGI1 had two alternative splicing forms, GmGI1α and GmGI1β. GmFKF1/2 transcripts were diurnally regulated, with a peak at zeitgeber time 12 (ZT12) in long days and at ZT10 in short days. The diurnal phases between GmGIs transcript levels greatly differed. GmGI2 expression was regulated by both the circadian clock and photoperiod. But the rhythmic phases of GmGI1 and GmGI3 expression levels were mainly conferred by long days. GmFKFs shared similar spatio-temporal expression profiles with GmGIs in all of the tissue/organs in different developmental stages in both LD and SD. Both GmFKF and GmGI proteins were targeted to the nucleus. Yeast two hybrid assays showed GmFKF1/GmFKF2 interacted with GmGI1/GmGI2/GmCDF1 (CYCLING DOF FACTOR CDF1 homolog in soybean); and the LOV (Light, Oxygen, or Voltage) domain in GmFKF1/GmFKF2 played an important role in these interactions. N-terminus of GmGI2 was sufficient to mediate its interaction with GmCDF1. Interestingly, N-terminus not full of GmGI3 interacted with GmFKF1/GmFKF2/GmCDF1. Ectopic over-expression of the GmFKF1 or GmFKF2 in Arabidopsis enhanced flowering in SD. Collectively, GmFKF and GmGI in soybean had conserved functional domains at DNA sequence level, but specific characters at function level with their homologs in other plants. |
| doi_str_mv | 10.1371/journal.pone.0079036 |
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But related reports are limited for soybean. In this study, we cloned FKF1 and GI homologs in soybean, and named as GmFKF1, GmFKF2, GmGI1, GmGI2, and GmGI3, respectively. GmGI1 had two alternative splicing forms, GmGI1α and GmGI1β. GmFKF1/2 transcripts were diurnally regulated, with a peak at zeitgeber time 12 (ZT12) in long days and at ZT10 in short days. The diurnal phases between GmGIs transcript levels greatly differed. GmGI2 expression was regulated by both the circadian clock and photoperiod. But the rhythmic phases of GmGI1 and GmGI3 expression levels were mainly conferred by long days. GmFKFs shared similar spatio-temporal expression profiles with GmGIs in all of the tissue/organs in different developmental stages in both LD and SD. Both GmFKF and GmGI proteins were targeted to the nucleus. Yeast two hybrid assays showed GmFKF1/GmFKF2 interacted with GmGI1/GmGI2/GmCDF1 (CYCLING DOF FACTOR CDF1 homolog in soybean); and the LOV (Light, Oxygen, or Voltage) domain in GmFKF1/GmFKF2 played an important role in these interactions. N-terminus of GmGI2 was sufficient to mediate its interaction with GmCDF1. Interestingly, N-terminus not full of GmGI3 interacted with GmFKF1/GmFKF2/GmCDF1. Ectopic over-expression of the GmFKF1 or GmFKF2 in Arabidopsis enhanced flowering in SD. Collectively, GmFKF and GmGI in soybean had conserved functional domains at DNA sequence level, but specific characters at function level with their homologs in other plants.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0079036</identifier><identifier>PMID: 24236086</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Alternative splicing ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis thaliana ; Binding sites ; Biology ; Cell Nucleus ; Circadian Clocks ; Circadian rhythm ; Circadian rhythms ; Conserved sequence ; Crop science ; Deoxyribonucleic acid ; Developmental stages ; Diurnal ; DNA ; Flavin ; Flowering ; Flowers - genetics ; Flowers - growth & development ; Gene Expression ; Gene Expression Regulation, Plant ; Genes ; Genes, Plant ; Genetic aspects ; Glycine max ; Glycine max - genetics ; Glycine max - metabolism ; Homology ; Laboratories ; Light ; N-Terminus ; Nuclei ; Nucleotide sequence ; Organ Specificity ; Organs ; Overexpression ; Oxygen ; Phylogeny ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified - growth & development ; Protein Interaction Mapping ; Protein Transport ; Proteins ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Homology, Amino Acid ; Soybeans ; Transcription ; Yeast ; Zeitgeber</subject><ispartof>PloS one, 2013-11, Vol.8 (11), p.e79036-e79036</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Li et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Li et al 2013 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-b248109daef18702626a24f4211c07f6279a76c5cd443419341aabc89b12d8843</citedby><cites>FETCH-LOGICAL-c758t-b248109daef18702626a24f4211c07f6279a76c5cd443419341aabc89b12d8843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3827303/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3827303/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53770,53772,79347,79348</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24236086$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sun, Meng-xiang</contributor><creatorcontrib>Li, Fang</creatorcontrib><creatorcontrib>Zhang, Xiaomei</creatorcontrib><creatorcontrib>Hu, Ruibo</creatorcontrib><creatorcontrib>Wu, Faqiang</creatorcontrib><creatorcontrib>Ma, Jinhua</creatorcontrib><creatorcontrib>Meng, Ying</creatorcontrib><creatorcontrib>Fu, Yongfu</creatorcontrib><title>Identification and molecular characterization of FKF1 and GI homologous genes in soybean</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In Arabidopsis, FKF1 (FLAVIN BINDING, KELCH REPEAT, F-BOX1) and GI (GIGANTEA) play important roles in flowering pathway through regulating daytime CO (CONSTANS) expression, and such a function is conserved across plants studied. But related reports are limited for soybean. In this study, we cloned FKF1 and GI homologs in soybean, and named as GmFKF1, GmFKF2, GmGI1, GmGI2, and GmGI3, respectively. GmGI1 had two alternative splicing forms, GmGI1α and GmGI1β. GmFKF1/2 transcripts were diurnally regulated, with a peak at zeitgeber time 12 (ZT12) in long days and at ZT10 in short days. The diurnal phases between GmGIs transcript levels greatly differed. GmGI2 expression was regulated by both the circadian clock and photoperiod. But the rhythmic phases of GmGI1 and GmGI3 expression levels were mainly conferred by long days. GmFKFs shared similar spatio-temporal expression profiles with GmGIs in all of the tissue/organs in different developmental stages in both LD and SD. Both GmFKF and GmGI proteins were targeted to the nucleus. Yeast two hybrid assays showed GmFKF1/GmFKF2 interacted with GmGI1/GmGI2/GmCDF1 (CYCLING DOF FACTOR CDF1 homolog in soybean); and the LOV (Light, Oxygen, or Voltage) domain in GmFKF1/GmFKF2 played an important role in these interactions. N-terminus of GmGI2 was sufficient to mediate its interaction with GmCDF1. Interestingly, N-terminus not full of GmGI3 interacted with GmFKF1/GmFKF2/GmCDF1. Ectopic over-expression of the GmFKF1 or GmFKF2 in Arabidopsis enhanced flowering in SD. Collectively, GmFKF and GmGI in soybean had conserved functional domains at DNA sequence level, but specific characters at function level with their homologs in other plants.</description><subject>Alternative splicing</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis thaliana</subject><subject>Binding sites</subject><subject>Biology</subject><subject>Cell Nucleus</subject><subject>Circadian Clocks</subject><subject>Circadian rhythm</subject><subject>Circadian rhythms</subject><subject>Conserved sequence</subject><subject>Crop science</subject><subject>Deoxyribonucleic acid</subject><subject>Developmental stages</subject><subject>Diurnal</subject><subject>DNA</subject><subject>Flavin</subject><subject>Flowering</subject><subject>Flowers - genetics</subject><subject>Flowers - growth & development</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genes, Plant</subject><subject>Genetic aspects</subject><subject>Glycine max</subject><subject>Glycine max - genetics</subject><subject>Glycine max - metabolism</subject><subject>Homology</subject><subject>Laboratories</subject><subject>Light</subject><subject>N-Terminus</subject><subject>Nuclei</subject><subject>Nucleotide sequence</subject><subject>Organ Specificity</subject><subject>Organs</subject><subject>Overexpression</subject><subject>Oxygen</subject><subject>Phylogeny</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified - growth & development</subject><subject>Protein Interaction Mapping</subject><subject>Protein Transport</subject><subject>Proteins</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>Soybeans</subject><subject>Transcription</subject><subject>Yeast</subject><subject>Zeitgeber</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLgujFjPlqPm6EZXHWwYUFv_AunKZpJ0unGZNWXH-96U53mcpeSC9Skue8J-fNOVn2HKMlpgK_u_JD6KBd7nxnlwgJhSh_kB1jRcmCE0QfHvwfZU9ivEKooJLzx9kRYYRyJPlx9mNd2a53tTPQO9_l0FX51rfWDC2E3GwggOltcH_2x77OV59W-AY7X-cbn1jf-CHmje1szF2XR39dWuieZo9qaKN9Nq0n2bfVh69nHxcXl-frs9OLhRGF7BclYRIjVYGtsRSIcMKBsJoRjA0SNSdCgeCmMBVjlKWCGAYojVQlJpWUjJ5kL_e6u9ZHPZkSNWaFJGOJIhHrPVF5uNK74LYQrrUHp282fGg0hN6Z1mqQpRW8MkrVnElplVLc8JQKMy4YKZPW-ynbUG5tZZJ3AdqZ6Pykcxvd-F-aSiIookngzSQQ_M_Bxl5vXTS2baGzycbx3goXBSUyoa_-Qe-vbqIaSAW4rvYprxlF9SkTkkipKE_U8h4qfZXdOpM6qHZpfxbwdhaQmN7-7hsYYtTrL5__n738PmdfH7AbC22_ib4dxu6Kc5DtQRN8jMHWdyZjpMcBuHVDjwOgpwFIYS8OH-gu6Lbj6V_msP6F</recordid><startdate>20131113</startdate><enddate>20131113</enddate><creator>Li, Fang</creator><creator>Zhang, Xiaomei</creator><creator>Hu, Ruibo</creator><creator>Wu, Faqiang</creator><creator>Ma, Jinhua</creator><creator>Meng, Ying</creator><creator>Fu, Yongfu</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131113</creationdate><title>Identification and molecular characterization of FKF1 and GI homologous genes in soybean</title><author>Li, Fang ; Zhang, Xiaomei ; Hu, Ruibo ; Wu, Faqiang ; Ma, Jinhua ; Meng, Ying ; Fu, Yongfu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-b248109daef18702626a24f4211c07f6279a76c5cd443419341aabc89b12d8843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Alternative splicing</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis thaliana</topic><topic>Binding sites</topic><topic>Biology</topic><topic>Cell Nucleus</topic><topic>Circadian Clocks</topic><topic>Circadian rhythm</topic><topic>Circadian rhythms</topic><topic>Conserved sequence</topic><topic>Crop science</topic><topic>Deoxyribonucleic acid</topic><topic>Developmental stages</topic><topic>Diurnal</topic><topic>DNA</topic><topic>Flavin</topic><topic>Flowering</topic><topic>Flowers - genetics</topic><topic>Flowers - growth & development</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genes, Plant</topic><topic>Genetic aspects</topic><topic>Glycine max</topic><topic>Glycine max - genetics</topic><topic>Glycine max - metabolism</topic><topic>Homology</topic><topic>Laboratories</topic><topic>Light</topic><topic>N-Terminus</topic><topic>Nuclei</topic><topic>Nucleotide sequence</topic><topic>Organ Specificity</topic><topic>Organs</topic><topic>Overexpression</topic><topic>Oxygen</topic><topic>Phylogeny</topic><topic>Plant Proteins - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Fang</au><au>Zhang, Xiaomei</au><au>Hu, Ruibo</au><au>Wu, Faqiang</au><au>Ma, Jinhua</au><au>Meng, Ying</au><au>Fu, Yongfu</au><au>Sun, Meng-xiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and molecular characterization of FKF1 and GI homologous genes in soybean</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-11-13</date><risdate>2013</risdate><volume>8</volume><issue>11</issue><spage>e79036</spage><epage>e79036</epage><pages>e79036-e79036</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In Arabidopsis, FKF1 (FLAVIN BINDING, KELCH REPEAT, F-BOX1) and GI (GIGANTEA) play important roles in flowering pathway through regulating daytime CO (CONSTANS) expression, and such a function is conserved across plants studied. But related reports are limited for soybean. In this study, we cloned FKF1 and GI homologs in soybean, and named as GmFKF1, GmFKF2, GmGI1, GmGI2, and GmGI3, respectively. GmGI1 had two alternative splicing forms, GmGI1α and GmGI1β. GmFKF1/2 transcripts were diurnally regulated, with a peak at zeitgeber time 12 (ZT12) in long days and at ZT10 in short days. The diurnal phases between GmGIs transcript levels greatly differed. GmGI2 expression was regulated by both the circadian clock and photoperiod. But the rhythmic phases of GmGI1 and GmGI3 expression levels were mainly conferred by long days. GmFKFs shared similar spatio-temporal expression profiles with GmGIs in all of the tissue/organs in different developmental stages in both LD and SD. Both GmFKF and GmGI proteins were targeted to the nucleus. Yeast two hybrid assays showed GmFKF1/GmFKF2 interacted with GmGI1/GmGI2/GmCDF1 (CYCLING DOF FACTOR CDF1 homolog in soybean); and the LOV (Light, Oxygen, or Voltage) domain in GmFKF1/GmFKF2 played an important role in these interactions. N-terminus of GmGI2 was sufficient to mediate its interaction with GmCDF1. Interestingly, N-terminus not full of GmGI3 interacted with GmFKF1/GmFKF2/GmCDF1. Ectopic over-expression of the GmFKF1 or GmFKF2 in Arabidopsis enhanced flowering in SD. Collectively, GmFKF and GmGI in soybean had conserved functional domains at DNA sequence level, but specific characters at function level with their homologs in other plants.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24236086</pmid><doi>10.1371/journal.pone.0079036</doi><tpages>e79036</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1458260867 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; Full-Text Journals in Chemistry (Open access); PubMed Central; Directory of Open Access Journals; EZB Electronic Journals Library |
| subjects | Alternative splicing Arabidopsis Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis thaliana Binding sites Biology Cell Nucleus Circadian Clocks Circadian rhythm Circadian rhythms Conserved sequence Crop science Deoxyribonucleic acid Developmental stages Diurnal DNA Flavin Flowering Flowers - genetics Flowers - growth & development Gene Expression Gene Expression Regulation, Plant Genes Genes, Plant Genetic aspects Glycine max Glycine max - genetics Glycine max - metabolism Homology Laboratories Light N-Terminus Nuclei Nucleotide sequence Organ Specificity Organs Overexpression Oxygen Phylogeny Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified - growth & development Protein Interaction Mapping Protein Transport Proteins RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Homology, Amino Acid Soybeans Transcription Yeast Zeitgeber |
| title | Identification and molecular characterization of FKF1 and GI homologous genes in soybean |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T17%3A48%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20and%20molecular%20characterization%20of%20FKF1%20and%20GI%20homologous%20genes%20in%20soybean&rft.jtitle=PloS%20one&rft.au=Li,%20Fang&rft.date=2013-11-13&rft.volume=8&rft.issue=11&rft.spage=e79036&rft.epage=e79036&rft.pages=e79036-e79036&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0079036&rft_dat=%3Cgale_plos_%3EA478288936%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1458260867&rft_id=info:pmid/24236086&rft_galeid=A478288936&rft_doaj_id=oai_doaj_org_article_a8be76dc99f6488e9996c6b12146742b&rfr_iscdi=true |