Cleavage events and sperm dynamics in chick intrauterine embryos
This study was undertaken to elucidate detailed event of early embryogenesis in chicken embryos using a noninvasive egg retrieval technique before oviposition. White Leghorn intrauterine eggs were retrieved from 95 cyclic hens aged up to 54-56 weeks and morphogenetic observation was made under both...
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| creator | Lee, Hyung Chul Choi, Hee Jung Park, Tae Sub Lee, Sang In Kim, Young Min Rengaraj, Deivendran Nagai, Hiroki Sheng, Guojun Lim, Jeong Mook Han, Jae Yong |
| description | This study was undertaken to elucidate detailed event of early embryogenesis in chicken embryos using a noninvasive egg retrieval technique before oviposition. White Leghorn intrauterine eggs were retrieved from 95 cyclic hens aged up to 54-56 weeks and morphogenetic observation was made under both bright field and fluorescent image in a time course manner. Differing from mammals, asymmetric cleavage to yield preblastodermal cells was observed throughout early embryogenesis. The first two divisions occurred synchronously and four polarized preblastodermal cells resulted after cruciform cleavage. Then, asynchronous cleavage continued in a radial manner and overall cell size in the initial cleavage region was smaller than that in the distal area. Numerous sperms were visible, regardless of zygotic nuclei formation. Condensed sperm heads were present mainly in the perivitelline space and cytoplasm, and rarely in the yolk region, while decondensed sperm heads were only visible in the yolk. In conclusion, apparent differences in sperm dynamics and early cleavage events compared with mammalian embryos were detected in chick embryo development, which demonstrated polarized cleavage with penetrating supernumerary sperm into multiple regions. |
| doi_str_mv | 10.1371/journal.pone.0080631 |
| format | Article |
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White Leghorn intrauterine eggs were retrieved from 95 cyclic hens aged up to 54-56 weeks and morphogenetic observation was made under both bright field and fluorescent image in a time course manner. Differing from mammals, asymmetric cleavage to yield preblastodermal cells was observed throughout early embryogenesis. The first two divisions occurred synchronously and four polarized preblastodermal cells resulted after cruciform cleavage. Then, asynchronous cleavage continued in a radial manner and overall cell size in the initial cleavage region was smaller than that in the distal area. Numerous sperms were visible, regardless of zygotic nuclei formation. Condensed sperm heads were present mainly in the perivitelline space and cytoplasm, and rarely in the yolk region, while decondensed sperm heads were only visible in the yolk. In conclusion, apparent differences in sperm dynamics and early cleavage events compared with mammalian embryos were detected in chick embryo development, which demonstrated polarized cleavage with penetrating supernumerary sperm into multiple regions.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0080631</identifier><identifier>PMID: 24244702</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abdomen ; Agricultural biotechnology ; Agriculture ; Animals ; Cell size ; Chick Embryo ; Chickens ; Cleavage ; Cytokinesis - physiology ; Cytoplasm ; Developmental biology ; Eggs ; Embryo, Nonmammalian - cytology ; Embryo, Nonmammalian - metabolism ; Embryo, Nonmammalian - physiology ; Embryogenesis ; Embryonic development ; Embryonic growth stage ; Embryos ; Experiments ; Female ; Fluorescence ; Laboratories ; Life sciences ; Male ; Nuclei ; Oviposition ; Ovum - cytology ; Ovum - metabolism ; Poultry ; Sperm ; Sperm-Ovum Interactions - physiology ; Spermatozoa - physiology ; Supernumerary ; Yolk</subject><ispartof>PloS one, 2013-11, Vol.8 (11), p.e80631-e80631</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Lee et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Lee et al 2013 Lee et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-a92d7b94e51654fbc031c753b8938adb6d2a2381593f57bd974da86632663bc3</citedby><cites>FETCH-LOGICAL-c692t-a92d7b94e51654fbc031c753b8938adb6d2a2381593f57bd974da86632663bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820643/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820643/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24244702$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Chavatte-Palmer, Pascale</contributor><creatorcontrib>Lee, Hyung Chul</creatorcontrib><creatorcontrib>Choi, Hee Jung</creatorcontrib><creatorcontrib>Park, Tae Sub</creatorcontrib><creatorcontrib>Lee, Sang In</creatorcontrib><creatorcontrib>Kim, Young Min</creatorcontrib><creatorcontrib>Rengaraj, Deivendran</creatorcontrib><creatorcontrib>Nagai, Hiroki</creatorcontrib><creatorcontrib>Sheng, Guojun</creatorcontrib><creatorcontrib>Lim, Jeong Mook</creatorcontrib><creatorcontrib>Han, Jae Yong</creatorcontrib><title>Cleavage events and sperm dynamics in chick intrauterine embryos</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>This study was undertaken to elucidate detailed event of early embryogenesis in chicken embryos using a noninvasive egg retrieval technique before oviposition. White Leghorn intrauterine eggs were retrieved from 95 cyclic hens aged up to 54-56 weeks and morphogenetic observation was made under both bright field and fluorescent image in a time course manner. Differing from mammals, asymmetric cleavage to yield preblastodermal cells was observed throughout early embryogenesis. The first two divisions occurred synchronously and four polarized preblastodermal cells resulted after cruciform cleavage. Then, asynchronous cleavage continued in a radial manner and overall cell size in the initial cleavage region was smaller than that in the distal area. Numerous sperms were visible, regardless of zygotic nuclei formation. Condensed sperm heads were present mainly in the perivitelline space and cytoplasm, and rarely in the yolk region, while decondensed sperm heads were only visible in the yolk. In conclusion, apparent differences in sperm dynamics and early cleavage events compared with mammalian embryos were detected in chick embryo development, which demonstrated polarized cleavage with penetrating supernumerary sperm into multiple regions.</description><subject>Abdomen</subject><subject>Agricultural biotechnology</subject><subject>Agriculture</subject><subject>Animals</subject><subject>Cell size</subject><subject>Chick Embryo</subject><subject>Chickens</subject><subject>Cleavage</subject><subject>Cytokinesis - physiology</subject><subject>Cytoplasm</subject><subject>Developmental biology</subject><subject>Eggs</subject><subject>Embryo, Nonmammalian - cytology</subject><subject>Embryo, Nonmammalian - metabolism</subject><subject>Embryo, Nonmammalian - physiology</subject><subject>Embryogenesis</subject><subject>Embryonic development</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Experiments</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Laboratories</subject><subject>Life sciences</subject><subject>Male</subject><subject>Nuclei</subject><subject>Oviposition</subject><subject>Ovum - cytology</subject><subject>Ovum - metabolism</subject><subject>Poultry</subject><subject>Sperm</subject><subject>Sperm-Ovum Interactions - physiology</subject><subject>Spermatozoa - physiology</subject><subject>Supernumerary</subject><subject>Yolk</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2LEzEUhgdR3LX6D0QHBNGL1nxPciMuxY_CwoIu3oZ8TZt1ZtJNZor996Z2dunIXkgIOSTPeZOT8xbFSwgWEFfww00YYqeaxTZ0bgEABwzDR8U5FBjNGQL48Ul8VjxL6QYAijljT4szRBAhFUDnxadl49ROrV3pdq7rU6k6W6ati21p951qvUml70qz8eZXDvqoht5F32W-1XEf0vPiSa2a5F6M66y4_vL5evltfnn1dbW8uJwbJlA_VwLZSgviKGSU1NoADE1FseYCc2U1s0ghzCEVuKaVtqIiVuXHYpSnNnhWvD7KbpuQ5Fh7kpAQgTFkDGVidSRsUDdyG32r4l4G5eXfjRDXUsXem8ZJbBy3mjBQQUQ40wJyagXHmkLtMAdZ6-N426BbZ407FN5MRKcnnd_IddhJzBFgBGeBd6NADLeDS71sfTKuaVTnwnB4NxWUVjQ3bVa8-Qd9uLqRWqtcgO_qkO81B1F5QSqOBaJCZGrxAJWHdbmT2Si1z_uThPeThMz07ne_VkNKcvXj-_-zVz-n7NsTduNU029SaIbehy5NQXIETQwpRVfffzIE8uDzu9-QB5_L0ec57dVpg-6T7oyN_wD1IPYl</recordid><startdate>20131107</startdate><enddate>20131107</enddate><creator>Lee, Hyung Chul</creator><creator>Choi, Hee Jung</creator><creator>Park, Tae Sub</creator><creator>Lee, Sang In</creator><creator>Kim, Young Min</creator><creator>Rengaraj, Deivendran</creator><creator>Nagai, Hiroki</creator><creator>Sheng, Guojun</creator><creator>Lim, Jeong Mook</creator><creator>Han, Jae Yong</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131107</creationdate><title>Cleavage events and sperm dynamics in chick intrauterine embryos</title><author>Lee, Hyung Chul ; Choi, Hee Jung ; Park, Tae Sub ; Lee, Sang In ; Kim, Young Min ; Rengaraj, Deivendran ; Nagai, Hiroki ; Sheng, Guojun ; Lim, Jeong Mook ; Han, Jae Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-a92d7b94e51654fbc031c753b8938adb6d2a2381593f57bd974da86632663bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Abdomen</topic><topic>Agricultural biotechnology</topic><topic>Agriculture</topic><topic>Animals</topic><topic>Cell size</topic><topic>Chick Embryo</topic><topic>Chickens</topic><topic>Cleavage</topic><topic>Cytokinesis - 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White Leghorn intrauterine eggs were retrieved from 95 cyclic hens aged up to 54-56 weeks and morphogenetic observation was made under both bright field and fluorescent image in a time course manner. Differing from mammals, asymmetric cleavage to yield preblastodermal cells was observed throughout early embryogenesis. The first two divisions occurred synchronously and four polarized preblastodermal cells resulted after cruciform cleavage. Then, asynchronous cleavage continued in a radial manner and overall cell size in the initial cleavage region was smaller than that in the distal area. Numerous sperms were visible, regardless of zygotic nuclei formation. Condensed sperm heads were present mainly in the perivitelline space and cytoplasm, and rarely in the yolk region, while decondensed sperm heads were only visible in the yolk. In conclusion, apparent differences in sperm dynamics and early cleavage events compared with mammalian embryos were detected in chick embryo development, which demonstrated polarized cleavage with penetrating supernumerary sperm into multiple regions.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24244702</pmid><doi>10.1371/journal.pone.0080631</doi><tpages>e80631</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Abdomen Agricultural biotechnology Agriculture Animals Cell size Chick Embryo Chickens Cleavage Cytokinesis - physiology Cytoplasm Developmental biology Eggs Embryo, Nonmammalian - cytology Embryo, Nonmammalian - metabolism Embryo, Nonmammalian - physiology Embryogenesis Embryonic development Embryonic growth stage Embryos Experiments Female Fluorescence Laboratories Life sciences Male Nuclei Oviposition Ovum - cytology Ovum - metabolism Poultry Sperm Sperm-Ovum Interactions - physiology Spermatozoa - physiology Supernumerary Yolk |
| title | Cleavage events and sperm dynamics in chick intrauterine embryos |
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