Use of nonelectrolytes reveals the channel size and oligomeric constitution of the Borrelia burgdorferi P66 porin

In the Lyme disease spirochete Borrelia burgdorferi, the outer membrane protein P66 is capable of pore formation with an atypical high single-channel conductance of 11 nS in 1 M KCl, which suggested that it could have a larger diameter than 'normal' Gram-negative bacterial porins. We studi...

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Veröffentlicht in:	PloS one 2013-11, Vol.8 (11), p.e78272-e78272
Hauptverfasser:	Bárcena-Uribarri, Iván, Thein, Marcus, Maier, Elke, Bonde, Mari, Bergström, Sven, Benz, Roland
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Bacterial Proteins - chemistry Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Biology black lipid bilayer Blockage Borrelia burgdorferi Borrelia burgdorferi - chemistry Cell Membrane - chemistry Chemical reactions Chlorides Conductance Constitution E coli Electric Conductivity Electrophoresis, Polyacrylamide Gel Engineering schools Escherichia coli Experiments Gel electrophoresis Gram-negative bacteria Immunoblotting Lipid bilayers Lipid Bilayers - chemistry Lipid Bilayers - metabolism Lipids Lyme disease Maltose - chemistry Medicine Membrane conductance Membrane Potentials - physiology Membrane proteins Membranes Molecular biology Noise Nonelectrolytes nutrient uptake Oligosaccharides - chemistry outer membrane transport Polyethylene glycol Polyethylene Glycols - chemistry Pore formation porin radius determination Porins Porins - chemistry Porins - isolation & purification Porins - metabolism Potassium chloride Potassium Chloride - chemistry Protein Multimerization Proteins Resistance Sodium lauryl sulfate Spirochetes Vector-borne diseases
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