An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays
Cell-based assays (CBA) have increased the sensitivity of the neuromyelitis optica (NMO)-IgG/aquaporin-4-antibody detection compared to classical tissue-based indirect assays. We describe the sensitivity of an optimized immunohistochemistry (IHC-o) to detect NMO-IgG/aquaporin-4-antibody in compariso...
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| creator | Höftberger, Romana Sabater, Lidia Marignier, Romain Aboul-Enein, Fahmy Bernard-Valnet, Raphaël Rauschka, Helmut Ruiz, Anne Blanco, Yolanda Graus, Francesc Dalmau, Josep Saiz, Albert |
| description | Cell-based assays (CBA) have increased the sensitivity of the neuromyelitis optica (NMO)-IgG/aquaporin-4-antibody detection compared to classical tissue-based indirect assays. We describe the sensitivity of an optimized immunohistochemistry (IHC-o) to detect NMO-IgG/aquaporin-4-antibody in comparison with that of two CBA: an in-house (CBA-ih) and a commercial (CBA-c) assay (Euroimmun, Germany). Coded serum from 103 patients with definite NMO and 122 inflammatory controls were studied by IHC-o, CBA-ih, and CBA-c. IHC-o used the same protocol described to detect antibodies against cell surface antigens. CBA-ih used live cells transfected with the aquaporin-4-M23-isoform. The sensitivity of the IHC-o was 74.8% (95% confidence interval [CI] 65-83) and was similar to that of the CBA-ih 75.7% (95% CI 66-84) and the CBA-c 73.8% (95% CI 64-82). The specificity of the three assays was 100% (95% CI 97-100). Interassay concordance was high, 100 of 103 samples were coincident in all techniques. The optimized immunohistochemistry proves to be as sensitive and specific as the cell-based assays. This assay extends the available tools for NMO-IgG/aquaporin-4-antibody detection. |
| doi_str_mv | 10.1371/journal.pone.0079083 |
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We describe the sensitivity of an optimized immunohistochemistry (IHC-o) to detect NMO-IgG/aquaporin-4-antibody in comparison with that of two CBA: an in-house (CBA-ih) and a commercial (CBA-c) assay (Euroimmun, Germany). Coded serum from 103 patients with definite NMO and 122 inflammatory controls were studied by IHC-o, CBA-ih, and CBA-c. IHC-o used the same protocol described to detect antibodies against cell surface antigens. CBA-ih used live cells transfected with the aquaporin-4-M23-isoform. The sensitivity of the IHC-o was 74.8% (95% confidence interval [CI] 65-83) and was similar to that of the CBA-ih 75.7% (95% CI 66-84) and the CBA-c 73.8% (95% CI 64-82). The specificity of the three assays was 100% (95% CI 97-100). Interassay concordance was high, 100 of 103 samples were coincident in all techniques. The optimized immunohistochemistry proves to be as sensitive and specific as the cell-based assays. This assay extends the available tools for NMO-IgG/aquaporin-4-antibody detection.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0079083</identifier><identifier>PMID: 24223884</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Animals ; Antibodies ; Antigens ; Aquaporin 4 ; Aquaporin 4 - immunology ; Aquaporins ; Assaying ; Autoantibodies - analysis ; Autoantibodies - immunology ; Cell surface ; Child ; Comparative analysis ; Confidence intervals ; Encephalitis ; Female ; Fluorescent Antibody Technique, Indirect - methods ; Hospitals ; Humans ; Immunoglobulin G ; Immunoglobulin G - analysis ; Immunoglobulin G - immunology ; Immunoglobulines ; Immunoglobulins ; Immunohistochemistry ; Immunohistochemistry - methods ; Immunohistoquímica ; Inflammation ; Life Sciences ; Male ; Medical diagnosis ; Medical research ; Methods ; Middle Aged ; Multiple sclerosis ; Neurology ; Neuromyelitis ; Neuromyelitis Optica - diagnosis ; Neuromyelitis Optica - immunology ; Neurosciences ; Patients ; Rats ; Rats, Sprague-Dawley ; Reproducibility of Results ; Sensitivity ; Sensitivity and Specificity ; Surface antigens ; Young Adult</subject><ispartof>PloS one, 2013-11, Vol.8 (11), p.e79083-e79083</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Höftberger et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>cc-by (c) Höftberger, Romana et al., 2013 info:eu-repo/semantics/openAccess <a href="http://creativecommons.org/licenses/by/3.0/es">http://creativecommons.org/licenses/by/3.0/es</a></rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>2013 Höftberger et al 2013 Höftberger et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c768t-132edee56f8aca59c2fbfbc381b3c41dbbd94966c0cdab400dda274b3c4221e23</citedby><cites>FETCH-LOGICAL-c768t-132edee56f8aca59c2fbfbc381b3c41dbbd94966c0cdab400dda274b3c4221e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817095/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817095/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,26953,27903,27904,53770,53772,79347,79348</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24223884$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04783564$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>Linker, Ralf Andreas</contributor><creatorcontrib>Höftberger, Romana</creatorcontrib><creatorcontrib>Sabater, Lidia</creatorcontrib><creatorcontrib>Marignier, Romain</creatorcontrib><creatorcontrib>Aboul-Enein, Fahmy</creatorcontrib><creatorcontrib>Bernard-Valnet, Raphaël</creatorcontrib><creatorcontrib>Rauschka, Helmut</creatorcontrib><creatorcontrib>Ruiz, Anne</creatorcontrib><creatorcontrib>Blanco, Yolanda</creatorcontrib><creatorcontrib>Graus, Francesc</creatorcontrib><creatorcontrib>Dalmau, Josep</creatorcontrib><creatorcontrib>Saiz, Albert</creatorcontrib><title>An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Cell-based assays (CBA) have increased the sensitivity of the neuromyelitis optica (NMO)-IgG/aquaporin-4-antibody detection compared to classical tissue-based indirect assays. We describe the sensitivity of an optimized immunohistochemistry (IHC-o) to detect NMO-IgG/aquaporin-4-antibody in comparison with that of two CBA: an in-house (CBA-ih) and a commercial (CBA-c) assay (Euroimmun, Germany). Coded serum from 103 patients with definite NMO and 122 inflammatory controls were studied by IHC-o, CBA-ih, and CBA-c. IHC-o used the same protocol described to detect antibodies against cell surface antigens. CBA-ih used live cells transfected with the aquaporin-4-M23-isoform. The sensitivity of the IHC-o was 74.8% (95% confidence interval [CI] 65-83) and was similar to that of the CBA-ih 75.7% (95% CI 66-84) and the CBA-c 73.8% (95% CI 64-82). The specificity of the three assays was 100% (95% CI 97-100). Interassay concordance was high, 100 of 103 samples were coincident in all techniques. The optimized immunohistochemistry proves to be as sensitive and specific as the cell-based assays. 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Academic</collection><collection>Recercat</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Höftberger, Romana</au><au>Sabater, Lidia</au><au>Marignier, Romain</au><au>Aboul-Enein, Fahmy</au><au>Bernard-Valnet, Raphaël</au><au>Rauschka, Helmut</au><au>Ruiz, Anne</au><au>Blanco, Yolanda</au><au>Graus, Francesc</au><au>Dalmau, Josep</au><au>Saiz, Albert</au><au>Linker, Ralf Andreas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-11-04</date><risdate>2013</risdate><volume>8</volume><issue>11</issue><spage>e79083</spage><epage>e79083</epage><pages>e79083-e79083</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Cell-based assays (CBA) have increased the sensitivity of the neuromyelitis optica (NMO)-IgG/aquaporin-4-antibody detection compared to classical tissue-based indirect assays. We describe the sensitivity of an optimized immunohistochemistry (IHC-o) to detect NMO-IgG/aquaporin-4-antibody in comparison with that of two CBA: an in-house (CBA-ih) and a commercial (CBA-c) assay (Euroimmun, Germany). Coded serum from 103 patients with definite NMO and 122 inflammatory controls were studied by IHC-o, CBA-ih, and CBA-c. IHC-o used the same protocol described to detect antibodies against cell surface antigens. CBA-ih used live cells transfected with the aquaporin-4-M23-isoform. The sensitivity of the IHC-o was 74.8% (95% confidence interval [CI] 65-83) and was similar to that of the CBA-ih 75.7% (95% CI 66-84) and the CBA-c 73.8% (95% CI 64-82). The specificity of the three assays was 100% (95% CI 97-100). Interassay concordance was high, 100 of 103 samples were coincident in all techniques. The optimized immunohistochemistry proves to be as sensitive and specific as the cell-based assays. This assay extends the available tools for NMO-IgG/aquaporin-4-antibody detection.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24223884</pmid><doi>10.1371/journal.pone.0079083</doi><tpages>e79083</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-11, Vol.8 (11), p.e79083-e79083 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1448416566 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); Recercat; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adolescent Adult Aged Aged, 80 and over Animals Antibodies Antigens Aquaporin 4 Aquaporin 4 - immunology Aquaporins Assaying Autoantibodies - analysis Autoantibodies - immunology Cell surface Child Comparative analysis Confidence intervals Encephalitis Female Fluorescent Antibody Technique, Indirect - methods Hospitals Humans Immunoglobulin G Immunoglobulin G - analysis Immunoglobulin G - immunology Immunoglobulines Immunoglobulins Immunohistochemistry Immunohistochemistry - methods Immunohistoquímica Inflammation Life Sciences Male Medical diagnosis Medical research Methods Middle Aged Multiple sclerosis Neurology Neuromyelitis Neuromyelitis Optica - diagnosis Neuromyelitis Optica - immunology Neurosciences Patients Rats Rats, Sprague-Dawley Reproducibility of Results Sensitivity Sensitivity and Specificity Surface antigens Young Adult |
| title | An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays |
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