Ultra-soft PDMS-based magnetoactive elastomers as dynamic cell culture substrata
Mechanical cues such as extracellular matrix stiffness and movement have a major impact on cell differentiation and function. To replicate these biological features in vitro, soft substrata with tunable elasticity and the possibility for controlled surface translocation are desirable. Here we report...
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| Veröffentlicht in: | PloS one 2013-10, Vol.8 (10), p.e76196-e76196 |
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| creator | Mayer, Matthias Rabindranath, Raman Börner, Juliane Hörner, Eva Bentz, Alexander Salgado, Josefina Han, Hong Böse, Holger Probst, Jörn Shamonin, Mikhail Monkman, Gareth J Schlunck, Günther |
| description | Mechanical cues such as extracellular matrix stiffness and movement have a major impact on cell differentiation and function. To replicate these biological features in vitro, soft substrata with tunable elasticity and the possibility for controlled surface translocation are desirable. Here we report on the use of ultra-soft (Young's modulus |
| doi_str_mv | 10.1371/journal.pone.0076196 |
| format | Article |
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To replicate these biological features in vitro, soft substrata with tunable elasticity and the possibility for controlled surface translocation are desirable. Here we report on the use of ultra-soft (Young's modulus <100 kPa) PDMS-based magnetoactive elastomers (MAE) as suitable cell culture substrata. Soft non-viscous PDMS (<18 kPa) is produced using a modified extended crosslinker. MAEs are generated by embedding magnetic microparticles into a soft PDMS matrix. Both substrata yield an elasticity-dependent (14 vs. 100 kPa) modulation of α-smooth muscle actin expression in primary human fibroblasts. To allow for static or dynamic control of MAE material properties, we devise low magnetic field (≈40 mT) stimulation systems compatible with cell-culture environments. Magnetic field-instigated stiffening (14 to 200 kPa) of soft MAE enhances the spreading of primary human fibroblasts and decreases PAX-7 transcription in human mesenchymal stem cells. Pulsatile MAE movements are generated using oscillating magnetic fields and are well tolerated by adherent human fibroblasts. This MAE system provides spatial and temporal control of substratum material characteristics and permits novel designs when used as dynamic cell culture substrata or cell culture-coated actuator in tissue engineering applications or biomedical devices.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0076196</identifier><identifier>PMID: 24204603</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Actin ; Advantages ; Biomechanics ; Biomedical engineering ; Biomedical materials ; Cell culture ; Cell Culture Techniques ; Cell differentiation ; Composite materials ; Crosslinking ; Cues ; Dermis - cytology ; Dimethylpolysiloxanes - chemistry ; Dynamic control ; Elastic Modulus - radiation effects ; Elasticity ; Elastomers ; Elastomers - chemistry ; Electrical engineering ; Embedding ; Extracellular Matrix ; Fibroblasts ; Humans ; Hydrogels ; Information technology ; Magnetic Fields ; Magnetic properties ; Mechanical properties ; Mechanotransduction, Cellular - physiology ; Mechanotransduction, Cellular - radiation effects ; Mesenchyme ; Microparticles ; Modulus of elasticity ; Muscle proteins ; Muscles ; Nylons - chemistry ; Polydimethylsiloxane ; Silicone resins ; Silicones - chemistry ; Smart materials ; Smooth muscle ; Stem cells ; Stiffening ; Stiffness ; Tissue culture ; Tissue engineering ; Transcription ; Translocation</subject><ispartof>PloS one, 2013-10, Vol.8 (10), p.e76196-e76196</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Mayer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Mayer et al 2013 Mayer et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-7fa159d23263e31cecb2111cda5a3a50557dabe0f8477fd4af9e6fdd428599193</citedby><cites>FETCH-LOGICAL-c758t-7fa159d23263e31cecb2111cda5a3a50557dabe0f8477fd4af9e6fdd428599193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799858/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799858/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24204603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Costa-Rodrigues, João</contributor><creatorcontrib>Mayer, Matthias</creatorcontrib><creatorcontrib>Rabindranath, Raman</creatorcontrib><creatorcontrib>Börner, Juliane</creatorcontrib><creatorcontrib>Hörner, Eva</creatorcontrib><creatorcontrib>Bentz, Alexander</creatorcontrib><creatorcontrib>Salgado, Josefina</creatorcontrib><creatorcontrib>Han, Hong</creatorcontrib><creatorcontrib>Böse, Holger</creatorcontrib><creatorcontrib>Probst, Jörn</creatorcontrib><creatorcontrib>Shamonin, Mikhail</creatorcontrib><creatorcontrib>Monkman, Gareth J</creatorcontrib><creatorcontrib>Schlunck, Günther</creatorcontrib><title>Ultra-soft PDMS-based magnetoactive elastomers as dynamic cell culture substrata</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Mechanical cues such as extracellular matrix stiffness and movement have a major impact on cell differentiation and function. To replicate these biological features in vitro, soft substrata with tunable elasticity and the possibility for controlled surface translocation are desirable. Here we report on the use of ultra-soft (Young's modulus <100 kPa) PDMS-based magnetoactive elastomers (MAE) as suitable cell culture substrata. Soft non-viscous PDMS (<18 kPa) is produced using a modified extended crosslinker. MAEs are generated by embedding magnetic microparticles into a soft PDMS matrix. Both substrata yield an elasticity-dependent (14 vs. 100 kPa) modulation of α-smooth muscle actin expression in primary human fibroblasts. To allow for static or dynamic control of MAE material properties, we devise low magnetic field (≈40 mT) stimulation systems compatible with cell-culture environments. Magnetic field-instigated stiffening (14 to 200 kPa) of soft MAE enhances the spreading of primary human fibroblasts and decreases PAX-7 transcription in human mesenchymal stem cells. Pulsatile MAE movements are generated using oscillating magnetic fields and are well tolerated by adherent human fibroblasts. This MAE system provides spatial and temporal control of substratum material characteristics and permits novel designs when used as dynamic cell culture substrata or cell culture-coated actuator in tissue engineering applications or biomedical devices.</description><subject>Actin</subject><subject>Advantages</subject><subject>Biomechanics</subject><subject>Biomedical engineering</subject><subject>Biomedical materials</subject><subject>Cell culture</subject><subject>Cell Culture Techniques</subject><subject>Cell differentiation</subject><subject>Composite materials</subject><subject>Crosslinking</subject><subject>Cues</subject><subject>Dermis - cytology</subject><subject>Dimethylpolysiloxanes - chemistry</subject><subject>Dynamic control</subject><subject>Elastic Modulus - radiation effects</subject><subject>Elasticity</subject><subject>Elastomers</subject><subject>Elastomers - chemistry</subject><subject>Electrical engineering</subject><subject>Embedding</subject><subject>Extracellular Matrix</subject><subject>Fibroblasts</subject><subject>Humans</subject><subject>Hydrogels</subject><subject>Information technology</subject><subject>Magnetic Fields</subject><subject>Magnetic properties</subject><subject>Mechanical properties</subject><subject>Mechanotransduction, Cellular - physiology</subject><subject>Mechanotransduction, Cellular - radiation effects</subject><subject>Mesenchyme</subject><subject>Microparticles</subject><subject>Modulus of elasticity</subject><subject>Muscle proteins</subject><subject>Muscles</subject><subject>Nylons - chemistry</subject><subject>Polydimethylsiloxane</subject><subject>Silicone resins</subject><subject>Silicones - chemistry</subject><subject>Smart materials</subject><subject>Smooth muscle</subject><subject>Stem cells</subject><subject>Stiffening</subject><subject>Stiffness</subject><subject>Tissue culture</subject><subject>Tissue engineering</subject><subject>Transcription</subject><subject>Translocation</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkktv1DAUhSMEoqXwDxBEQkKwyGDHr3iDVJXXSEWtKGVr3fgxk1EmHmKnov8ez0xaTVAXyItYznfP9T0-WfYSoxkmAn9Y-aHvoJ1tfGdnCAmOJX-UHWNJyoKXiDw-2B9lz0JYIcRIxfnT7KikJaIckePs8rqNPRTBu5hffvp-VdQQrMnXsOhs9KBjc2Nz20KIfm37kEPIzW0H60bn2rZtroc2Dr3Nw1CHJBThefbEQRvsi_F7kl1_-fzz7FtxfvF1fnZ6XmjBqlgIB5hJU5KSE0uwtrouMcbaAAMCDDEmDNQWuYoK4QwFJy13xtCyYlKmwU6y13vdTeuDGs0IClNayirNtyXme8J4WKlN36yhv1UeGrU78P1CQR8b3VqFnUCEEWZrB9RyXhvBHWVC8HQDKiFpfRy7DfXaGm27NGw7EZ3-6ZqlWvgbRYSUFauSwLtRoPe_BxuiWjdh6yB01g-7e0vBBZI0oW_-QR-ebqQWkAZoOudTX70VVadUVGWyaNd29gCVlrHpCVNyXJPOJwXvJwWJifZPXMAQgppf_fh_9uLXlH17wC4ttHEZfDvExndhCtI9qHsfQm_dvckYqW3w79xQ2-CrMfip7NXhA90X3SWd_AUGQv3H</recordid><startdate>20131018</startdate><enddate>20131018</enddate><creator>Mayer, Matthias</creator><creator>Rabindranath, Raman</creator><creator>Börner, Juliane</creator><creator>Hörner, Eva</creator><creator>Bentz, Alexander</creator><creator>Salgado, Josefina</creator><creator>Han, Hong</creator><creator>Böse, Holger</creator><creator>Probst, Jörn</creator><creator>Shamonin, Mikhail</creator><creator>Monkman, Gareth J</creator><creator>Schlunck, Günther</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131018</creationdate><title>Ultra-soft PDMS-based magnetoactive elastomers as dynamic cell culture substrata</title><author>Mayer, Matthias ; Rabindranath, Raman ; Börner, Juliane ; Hörner, Eva ; Bentz, Alexander ; Salgado, Josefina ; Han, Hong ; Böse, Holger ; Probst, Jörn ; Shamonin, Mikhail ; Monkman, Gareth J ; Schlunck, Günther</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-7fa159d23263e31cecb2111cda5a3a50557dabe0f8477fd4af9e6fdd428599193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Actin</topic><topic>Advantages</topic><topic>Biomechanics</topic><topic>Biomedical engineering</topic><topic>Biomedical materials</topic><topic>Cell culture</topic><topic>Cell Culture Techniques</topic><topic>Cell differentiation</topic><topic>Composite materials</topic><topic>Crosslinking</topic><topic>Cues</topic><topic>Dermis - 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To replicate these biological features in vitro, soft substrata with tunable elasticity and the possibility for controlled surface translocation are desirable. Here we report on the use of ultra-soft (Young's modulus <100 kPa) PDMS-based magnetoactive elastomers (MAE) as suitable cell culture substrata. Soft non-viscous PDMS (<18 kPa) is produced using a modified extended crosslinker. MAEs are generated by embedding magnetic microparticles into a soft PDMS matrix. Both substrata yield an elasticity-dependent (14 vs. 100 kPa) modulation of α-smooth muscle actin expression in primary human fibroblasts. To allow for static or dynamic control of MAE material properties, we devise low magnetic field (≈40 mT) stimulation systems compatible with cell-culture environments. Magnetic field-instigated stiffening (14 to 200 kPa) of soft MAE enhances the spreading of primary human fibroblasts and decreases PAX-7 transcription in human mesenchymal stem cells. Pulsatile MAE movements are generated using oscillating magnetic fields and are well tolerated by adherent human fibroblasts. This MAE system provides spatial and temporal control of substratum material characteristics and permits novel designs when used as dynamic cell culture substrata or cell culture-coated actuator in tissue engineering applications or biomedical devices.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24204603</pmid><doi>10.1371/journal.pone.0076196</doi><tpages>e76196</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-10, Vol.8 (10), p.e76196-e76196 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1442982429 |
| source | PubMed Central Free; MEDLINE; Public Library of Science; DOAJ Directory of Open Access Journals; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Actin Advantages Biomechanics Biomedical engineering Biomedical materials Cell culture Cell Culture Techniques Cell differentiation Composite materials Crosslinking Cues Dermis - cytology Dimethylpolysiloxanes - chemistry Dynamic control Elastic Modulus - radiation effects Elasticity Elastomers Elastomers - chemistry Electrical engineering Embedding Extracellular Matrix Fibroblasts Humans Hydrogels Information technology Magnetic Fields Magnetic properties Mechanical properties Mechanotransduction, Cellular - physiology Mechanotransduction, Cellular - radiation effects Mesenchyme Microparticles Modulus of elasticity Muscle proteins Muscles Nylons - chemistry Polydimethylsiloxane Silicone resins Silicones - chemistry Smart materials Smooth muscle Stem cells Stiffening Stiffness Tissue culture Tissue engineering Transcription Translocation |
| title | Ultra-soft PDMS-based magnetoactive elastomers as dynamic cell culture substrata |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T20%3A00%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ultra-soft%20PDMS-based%20magnetoactive%20elastomers%20as%20dynamic%20cell%20culture%20substrata&rft.jtitle=PloS%20one&rft.au=Mayer,%20Matthias&rft.date=2013-10-18&rft.volume=8&rft.issue=10&rft.spage=e76196&rft.epage=e76196&rft.pages=e76196-e76196&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0076196&rft_dat=%3Cgale_plos_%3EA478219358%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1442982429&rft_id=info:pmid/24204603&rft_galeid=A478219358&rft_doaj_id=oai_doaj_org_article_1f703535ebfa4e66bd76f45776e0f49a&rfr_iscdi=true |