High prevalence and significance of hepatitis D virus infection among treatment-naïve HBsAg-positive patients in Northern Vietnam
Hepatitis D virus (HDV) infection is considered to cause more severe hepatitis than hepatitis B virus (HBV) monoinfection. With more than 9.5 million HBV-infected people, Vietnam will face an enormous health burden. The prevalence of HDV in Vietnamese HBsAg-positive patients is speculative. Therefor...
Gespeichert in:
| Veröffentlicht in: | PloS one 2013-10, Vol.8 (10), p.e78094-e78094 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e78094 |
|---|---|
| container_issue | 10 |
| container_start_page | e78094 |
| container_title | PloS one |
| container_volume | 8 |
| creator | Sy, Bui Tien Ratsch, Boris A Toan, Nguyen Linh Song, Le Huu Wollboldt, Christian Bryniok, Agnes Nguyen, Hung Minh Luong, Hoang Van Velavan, Thirumalaisamy P Wedemeyer, Heiner Kremsner, Peter G Bock, C-Thomas |
| description | Hepatitis D virus (HDV) infection is considered to cause more severe hepatitis than hepatitis B virus (HBV) monoinfection. With more than 9.5 million HBV-infected people, Vietnam will face an enormous health burden. The prevalence of HDV in Vietnamese HBsAg-positive patients is speculative. Therefore, we assessed the prevalence of HDV in Vietnamese patients, determined the HDV-genotype distribution and compared the findings with the clinical outcome.
266 sera of well-characterized HBsAg-positive patients in Northern Vietnam were analysed for the presence of HDV using newly developed HDV-specific RT-PCRs. Sequencing and phylogenetic analysis were performed for HDV-genotyping.
The HDV-genome prevalence observed in the Vietnamese HBsAg-positive patients was high with 15.4% while patients with acute hepatitis showed 43.3%. Phylogenetic analysis demonstrated a predominance of HDV-genotype 1 clustering in an Asian clade while HDV-genotype 2 could be also detected. The serum aminotransferase levels (AST, ALT) as well as total and direct bilirubin were significantly elevated in HDV-positive individuals (p |
| doi_str_mv | 10.1371/journal.pone.0078094 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_1442982252</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_3593f8f41ef54bd3a05feb0dc3fe93b6</doaj_id><sourcerecordid>1449766193</sourcerecordid><originalsourceid>FETCH-LOGICAL-c592t-1687449057e25c2a8900dfa1c1ff21721b7ff66b7351eaea38b2ba409839a8673</originalsourceid><addsrcrecordid>eNptUstu1DAUjRCIlsIfILDEhk0GP5I43iCV8phKFWyArXXjXGc8SuxgZ0Ziyw_xEfwYSWdatYiV7XvPOb7HPln2nNEVE5K92YZd9NCvxuBxRamsqSoeZKdMCZ5XnIqHd_Yn2ZOUtpSWoq6qx9kJLzgtGa1Os19r123IGHEPPXqDBHxLkuu8s87AUgiWbHCEyU0ukfdk7-IuEectmskFT2AIviNTRJgG9FPu4c_vPZL1u3Te5WNIM20-Lvy5uxDJ5xCnDUZPvjucPAxPs0cW-oTPjutZ9u3jh68X6_zqy6fLi_Or3JSKTzmralkUipYSeWk41IrS1gIzzFrOJGeNtLaqGilKhoAg6oY3UFBVCwV1JcVZ9vKgO_Yh6ePzJc2Kgqua85LPiMsDog2w1WN0A8SfOoDT14UQOw1xcqZHLUolbG0LhrYsmlYALS02tDXCohJNNWu9Pd62awZszew-Qn9P9H7Hu43uwl4LqZSU5Szw-igQw48dpkkPLhnse_AYdtdzK1lV8x_P0Ff_QP_vrjigTAwpRbS3wzCql0jdsPQSKX2M1Ex7cdfILekmQ-Ivba_M9g</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1442982252</pqid></control><display><type>article</type><title>High prevalence and significance of hepatitis D virus infection among treatment-naïve HBsAg-positive patients in Northern Vietnam</title><source>PLoS</source><source>MEDLINE</source><source>Full-Text Journals in Chemistry (Open access)</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central</source><source>EZB Electronic Journals Library</source><creator>Sy, Bui Tien ; Ratsch, Boris A ; Toan, Nguyen Linh ; Song, Le Huu ; Wollboldt, Christian ; Bryniok, Agnes ; Nguyen, Hung Minh ; Luong, Hoang Van ; Velavan, Thirumalaisamy P ; Wedemeyer, Heiner ; Kremsner, Peter G ; Bock, C-Thomas</creator><creatorcontrib>Sy, Bui Tien ; Ratsch, Boris A ; Toan, Nguyen Linh ; Song, Le Huu ; Wollboldt, Christian ; Bryniok, Agnes ; Nguyen, Hung Minh ; Luong, Hoang Van ; Velavan, Thirumalaisamy P ; Wedemeyer, Heiner ; Kremsner, Peter G ; Bock, C-Thomas</creatorcontrib><description>Hepatitis D virus (HDV) infection is considered to cause more severe hepatitis than hepatitis B virus (HBV) monoinfection. With more than 9.5 million HBV-infected people, Vietnam will face an enormous health burden. The prevalence of HDV in Vietnamese HBsAg-positive patients is speculative. Therefore, we assessed the prevalence of HDV in Vietnamese patients, determined the HDV-genotype distribution and compared the findings with the clinical outcome.
266 sera of well-characterized HBsAg-positive patients in Northern Vietnam were analysed for the presence of HDV using newly developed HDV-specific RT-PCRs. Sequencing and phylogenetic analysis were performed for HDV-genotyping.
The HDV-genome prevalence observed in the Vietnamese HBsAg-positive patients was high with 15.4% while patients with acute hepatitis showed 43.3%. Phylogenetic analysis demonstrated a predominance of HDV-genotype 1 clustering in an Asian clade while HDV-genotype 2 could be also detected. The serum aminotransferase levels (AST, ALT) as well as total and direct bilirubin were significantly elevated in HDV-positive individuals (p<0.05). HDV loads were mainly low (<300 to 4.108 HDV-copies/ml). Of note, higher HDV loads were mainly found in HBV-genotype mix samples in contrast to single HBV-infections. In HBV/HDV-coinfections, HBV loads were significantly higher in HBV-genotype C in comparison to HBV-genotype A samples (p<0.05).
HDV prevalence is high in Vietnamese individuals, especially in patients with acute hepatitis B. HDV replication activity showed a HBV-genotype dependency and could be associated with elevated liver parameters. Besides serological assays molecular tests are recommended for diagnosis of HDV. Finally, the high prevalence of HBV and HDV prompts the urgent need for HBV-vaccination coverage.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0078094</identifier><identifier>PMID: 24205106</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Aged ; Antigens ; Bilirubin ; Bilirubin - metabolism ; Clustering ; Epidemiology ; Female ; Gene sequencing ; Genomes ; Genotype ; Genotype & phenotype ; Genotyping ; Hepatitis ; Hepatitis B ; Hepatitis B surface antigen ; Hepatitis B Surface Antigens - metabolism ; Hepatitis D - epidemiology ; Hepatitis D - immunology ; Hepatitis D - metabolism ; Hepatitis D - virology ; Hepatitis delta virus ; Hepatitis Delta Virus - immunology ; Hepatitis Delta Virus - pathogenicity ; Hepatology ; Hospitals ; Humans ; Infections ; Infectious diseases ; Interferon ; Liver ; Liver cancer ; Liver diseases ; Loads (forces) ; Male ; Middle Aged ; Patients ; Phylogeny ; Prevalence ; Reverse Transcriptase Polymerase Chain Reaction ; Studies ; Vaccination ; Vietnam - epidemiology ; Viruses ; Young Adult</subject><ispartof>PloS one, 2013-10, Vol.8 (10), p.e78094-e78094</ispartof><rights>2013 Sy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Sy et al 2013 Sy et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-1687449057e25c2a8900dfa1c1ff21721b7ff66b7351eaea38b2ba409839a8673</citedby><cites>FETCH-LOGICAL-c592t-1687449057e25c2a8900dfa1c1ff21721b7ff66b7351eaea38b2ba409839a8673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799775/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799775/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24205106$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sy, Bui Tien</creatorcontrib><creatorcontrib>Ratsch, Boris A</creatorcontrib><creatorcontrib>Toan, Nguyen Linh</creatorcontrib><creatorcontrib>Song, Le Huu</creatorcontrib><creatorcontrib>Wollboldt, Christian</creatorcontrib><creatorcontrib>Bryniok, Agnes</creatorcontrib><creatorcontrib>Nguyen, Hung Minh</creatorcontrib><creatorcontrib>Luong, Hoang Van</creatorcontrib><creatorcontrib>Velavan, Thirumalaisamy P</creatorcontrib><creatorcontrib>Wedemeyer, Heiner</creatorcontrib><creatorcontrib>Kremsner, Peter G</creatorcontrib><creatorcontrib>Bock, C-Thomas</creatorcontrib><title>High prevalence and significance of hepatitis D virus infection among treatment-naïve HBsAg-positive patients in Northern Vietnam</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Hepatitis D virus (HDV) infection is considered to cause more severe hepatitis than hepatitis B virus (HBV) monoinfection. With more than 9.5 million HBV-infected people, Vietnam will face an enormous health burden. The prevalence of HDV in Vietnamese HBsAg-positive patients is speculative. Therefore, we assessed the prevalence of HDV in Vietnamese patients, determined the HDV-genotype distribution and compared the findings with the clinical outcome.
266 sera of well-characterized HBsAg-positive patients in Northern Vietnam were analysed for the presence of HDV using newly developed HDV-specific RT-PCRs. Sequencing and phylogenetic analysis were performed for HDV-genotyping.
The HDV-genome prevalence observed in the Vietnamese HBsAg-positive patients was high with 15.4% while patients with acute hepatitis showed 43.3%. Phylogenetic analysis demonstrated a predominance of HDV-genotype 1 clustering in an Asian clade while HDV-genotype 2 could be also detected. The serum aminotransferase levels (AST, ALT) as well as total and direct bilirubin were significantly elevated in HDV-positive individuals (p<0.05). HDV loads were mainly low (<300 to 4.108 HDV-copies/ml). Of note, higher HDV loads were mainly found in HBV-genotype mix samples in contrast to single HBV-infections. In HBV/HDV-coinfections, HBV loads were significantly higher in HBV-genotype C in comparison to HBV-genotype A samples (p<0.05).
HDV prevalence is high in Vietnamese individuals, especially in patients with acute hepatitis B. HDV replication activity showed a HBV-genotype dependency and could be associated with elevated liver parameters. Besides serological assays molecular tests are recommended for diagnosis of HDV. Finally, the high prevalence of HBV and HDV prompts the urgent need for HBV-vaccination coverage.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Antigens</subject><subject>Bilirubin</subject><subject>Bilirubin - metabolism</subject><subject>Clustering</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Gene sequencing</subject><subject>Genomes</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Genotyping</subject><subject>Hepatitis</subject><subject>Hepatitis B</subject><subject>Hepatitis B surface antigen</subject><subject>Hepatitis B Surface Antigens - metabolism</subject><subject>Hepatitis D - epidemiology</subject><subject>Hepatitis D - immunology</subject><subject>Hepatitis D - metabolism</subject><subject>Hepatitis D - virology</subject><subject>Hepatitis delta virus</subject><subject>Hepatitis Delta Virus - immunology</subject><subject>Hepatitis Delta Virus - pathogenicity</subject><subject>Hepatology</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Interferon</subject><subject>Liver</subject><subject>Liver cancer</subject><subject>Liver diseases</subject><subject>Loads (forces)</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Patients</subject><subject>Phylogeny</subject><subject>Prevalence</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Studies</subject><subject>Vaccination</subject><subject>Vietnam - epidemiology</subject><subject>Viruses</subject><subject>Young Adult</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptUstu1DAUjRCIlsIfILDEhk0GP5I43iCV8phKFWyArXXjXGc8SuxgZ0Ziyw_xEfwYSWdatYiV7XvPOb7HPln2nNEVE5K92YZd9NCvxuBxRamsqSoeZKdMCZ5XnIqHd_Yn2ZOUtpSWoq6qx9kJLzgtGa1Os19r123IGHEPPXqDBHxLkuu8s87AUgiWbHCEyU0ukfdk7-IuEectmskFT2AIviNTRJgG9FPu4c_vPZL1u3Te5WNIM20-Lvy5uxDJ5xCnDUZPvjucPAxPs0cW-oTPjutZ9u3jh68X6_zqy6fLi_Or3JSKTzmralkUipYSeWk41IrS1gIzzFrOJGeNtLaqGilKhoAg6oY3UFBVCwV1JcVZ9vKgO_Yh6ePzJc2Kgqua85LPiMsDog2w1WN0A8SfOoDT14UQOw1xcqZHLUolbG0LhrYsmlYALS02tDXCohJNNWu9Pd62awZszew-Qn9P9H7Hu43uwl4LqZSU5Szw-igQw48dpkkPLhnse_AYdtdzK1lV8x_P0Ff_QP_vrjigTAwpRbS3wzCql0jdsPQSKX2M1Ex7cdfILekmQ-Ivba_M9g</recordid><startdate>20131018</startdate><enddate>20131018</enddate><creator>Sy, Bui Tien</creator><creator>Ratsch, Boris A</creator><creator>Toan, Nguyen Linh</creator><creator>Song, Le Huu</creator><creator>Wollboldt, Christian</creator><creator>Bryniok, Agnes</creator><creator>Nguyen, Hung Minh</creator><creator>Luong, Hoang Van</creator><creator>Velavan, Thirumalaisamy P</creator><creator>Wedemeyer, Heiner</creator><creator>Kremsner, Peter G</creator><creator>Bock, C-Thomas</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131018</creationdate><title>High prevalence and significance of hepatitis D virus infection among treatment-naïve HBsAg-positive patients in Northern Vietnam</title><author>Sy, Bui Tien ; Ratsch, Boris A ; Toan, Nguyen Linh ; Song, Le Huu ; Wollboldt, Christian ; Bryniok, Agnes ; Nguyen, Hung Minh ; Luong, Hoang Van ; Velavan, Thirumalaisamy P ; Wedemeyer, Heiner ; Kremsner, Peter G ; Bock, C-Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c592t-1687449057e25c2a8900dfa1c1ff21721b7ff66b7351eaea38b2ba409839a8673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Antigens</topic><topic>Bilirubin</topic><topic>Bilirubin - metabolism</topic><topic>Clustering</topic><topic>Epidemiology</topic><topic>Female</topic><topic>Gene sequencing</topic><topic>Genomes</topic><topic>Genotype</topic><topic>Genotype & phenotype</topic><topic>Genotyping</topic><topic>Hepatitis</topic><topic>Hepatitis B</topic><topic>Hepatitis B surface antigen</topic><topic>Hepatitis B Surface Antigens - metabolism</topic><topic>Hepatitis D - epidemiology</topic><topic>Hepatitis D - immunology</topic><topic>Hepatitis D - metabolism</topic><topic>Hepatitis D - virology</topic><topic>Hepatitis delta virus</topic><topic>Hepatitis Delta Virus - immunology</topic><topic>Hepatitis Delta Virus - pathogenicity</topic><topic>Hepatology</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Interferon</topic><topic>Liver</topic><topic>Liver cancer</topic><topic>Liver diseases</topic><topic>Loads (forces)</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Patients</topic><topic>Phylogeny</topic><topic>Prevalence</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Studies</topic><topic>Vaccination</topic><topic>Vietnam - epidemiology</topic><topic>Viruses</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sy, Bui Tien</creatorcontrib><creatorcontrib>Ratsch, Boris A</creatorcontrib><creatorcontrib>Toan, Nguyen Linh</creatorcontrib><creatorcontrib>Song, Le Huu</creatorcontrib><creatorcontrib>Wollboldt, Christian</creatorcontrib><creatorcontrib>Bryniok, Agnes</creatorcontrib><creatorcontrib>Nguyen, Hung Minh</creatorcontrib><creatorcontrib>Luong, Hoang Van</creatorcontrib><creatorcontrib>Velavan, Thirumalaisamy P</creatorcontrib><creatorcontrib>Wedemeyer, Heiner</creatorcontrib><creatorcontrib>Kremsner, Peter G</creatorcontrib><creatorcontrib>Bock, C-Thomas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Database (1962 - current)</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sy, Bui Tien</au><au>Ratsch, Boris A</au><au>Toan, Nguyen Linh</au><au>Song, Le Huu</au><au>Wollboldt, Christian</au><au>Bryniok, Agnes</au><au>Nguyen, Hung Minh</au><au>Luong, Hoang Van</au><au>Velavan, Thirumalaisamy P</au><au>Wedemeyer, Heiner</au><au>Kremsner, Peter G</au><au>Bock, C-Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High prevalence and significance of hepatitis D virus infection among treatment-naïve HBsAg-positive patients in Northern Vietnam</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-10-18</date><risdate>2013</risdate><volume>8</volume><issue>10</issue><spage>e78094</spage><epage>e78094</epage><pages>e78094-e78094</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Hepatitis D virus (HDV) infection is considered to cause more severe hepatitis than hepatitis B virus (HBV) monoinfection. With more than 9.5 million HBV-infected people, Vietnam will face an enormous health burden. The prevalence of HDV in Vietnamese HBsAg-positive patients is speculative. Therefore, we assessed the prevalence of HDV in Vietnamese patients, determined the HDV-genotype distribution and compared the findings with the clinical outcome.
266 sera of well-characterized HBsAg-positive patients in Northern Vietnam were analysed for the presence of HDV using newly developed HDV-specific RT-PCRs. Sequencing and phylogenetic analysis were performed for HDV-genotyping.
The HDV-genome prevalence observed in the Vietnamese HBsAg-positive patients was high with 15.4% while patients with acute hepatitis showed 43.3%. Phylogenetic analysis demonstrated a predominance of HDV-genotype 1 clustering in an Asian clade while HDV-genotype 2 could be also detected. The serum aminotransferase levels (AST, ALT) as well as total and direct bilirubin were significantly elevated in HDV-positive individuals (p<0.05). HDV loads were mainly low (<300 to 4.108 HDV-copies/ml). Of note, higher HDV loads were mainly found in HBV-genotype mix samples in contrast to single HBV-infections. In HBV/HDV-coinfections, HBV loads were significantly higher in HBV-genotype C in comparison to HBV-genotype A samples (p<0.05).
HDV prevalence is high in Vietnamese individuals, especially in patients with acute hepatitis B. HDV replication activity showed a HBV-genotype dependency and could be associated with elevated liver parameters. Besides serological assays molecular tests are recommended for diagnosis of HDV. Finally, the high prevalence of HBV and HDV prompts the urgent need for HBV-vaccination coverage.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24205106</pmid><doi>10.1371/journal.pone.0078094</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-10, Vol.8 (10), p.e78094-e78094 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1442982252 |
| source | PLoS; MEDLINE; Full-Text Journals in Chemistry (Open access); DOAJ Directory of Open Access Journals; PubMed Central; EZB Electronic Journals Library |
| subjects | Adolescent Adult Aged Antigens Bilirubin Bilirubin - metabolism Clustering Epidemiology Female Gene sequencing Genomes Genotype Genotype & phenotype Genotyping Hepatitis Hepatitis B Hepatitis B surface antigen Hepatitis B Surface Antigens - metabolism Hepatitis D - epidemiology Hepatitis D - immunology Hepatitis D - metabolism Hepatitis D - virology Hepatitis delta virus Hepatitis Delta Virus - immunology Hepatitis Delta Virus - pathogenicity Hepatology Hospitals Humans Infections Infectious diseases Interferon Liver Liver cancer Liver diseases Loads (forces) Male Middle Aged Patients Phylogeny Prevalence Reverse Transcriptase Polymerase Chain Reaction Studies Vaccination Vietnam - epidemiology Viruses Young Adult |
| title | High prevalence and significance of hepatitis D virus infection among treatment-naïve HBsAg-positive patients in Northern Vietnam |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T19%3A54%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=High%20prevalence%20and%20significance%20of%20hepatitis%20D%20virus%20infection%20among%20treatment-na%C3%AFve%20HBsAg-positive%20patients%20in%20Northern%20Vietnam&rft.jtitle=PloS%20one&rft.au=Sy,%20Bui%20Tien&rft.date=2013-10-18&rft.volume=8&rft.issue=10&rft.spage=e78094&rft.epage=e78094&rft.pages=e78094-e78094&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0078094&rft_dat=%3Cproquest_plos_%3E1449766193%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1442982252&rft_id=info:pmid/24205106&rft_doaj_id=oai_doaj_org_article_3593f8f41ef54bd3a05feb0dc3fe93b6&rfr_iscdi=true |