Chronic academic stress increases a group of microRNAs in peripheral blood
MicroRNAs (miRNAs) play key roles in regulation of cellular processes in response to changes in environment. In this study, we examined alterations in miRNA profiles in peripheral blood from 25 male medical students two months and two days before the National Examination for Medical Practitioners. B...
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| Veröffentlicht in: | PloS one 2013-10, Vol.8 (10), p.e75960-e75960 |
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| creator | Honda, Manami Kuwano, Yuki Katsuura-Kamano, Sakurako Kamezaki, Yoshiko Fujita, Kinuyo Akaike, Yoko Kano, Shizuka Nishida, Kensei Masuda, Kiyoshi Rokutan, Kazuhito |
| description | MicroRNAs (miRNAs) play key roles in regulation of cellular processes in response to changes in environment. In this study, we examined alterations in miRNA profiles in peripheral blood from 25 male medical students two months and two days before the National Examination for Medical Practitioners. Blood obtained one month after the examination were used as baseline controls. Levels of seven miRNAs (miR-16, -20b, -26b, -29a, -126, -144 and -144*) were significantly elevated during the pre-examination period in association with significant down-regulation of their target mRNAs (WNT4, CCM2, MAK, and FGFR1 mRNAs) two days before the examination. State anxiety assessed two months before the examination was positively and negatively correlated with miR-16 and its target WNT4 mRNA levels, respectively. Fold changes in miR-16 levels from two days before to one month after the examination were inversely correlated with those in WNT4 mRNA levels over the same time points. We also confirmed the interaction between miR-16 and WNT4 3'UTR in HEK293T cells overexpressing FLAG-tagged WNT4 3'UTR and miR-16. Thus, a distinct group of miRNAs in periheral blood may participate in the integrated response to chronic academic stress in healthy young men. |
| doi_str_mv | 10.1371/journal.pone.0075960 |
| format | Article |
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In this study, we examined alterations in miRNA profiles in peripheral blood from 25 male medical students two months and two days before the National Examination for Medical Practitioners. Blood obtained one month after the examination were used as baseline controls. Levels of seven miRNAs (miR-16, -20b, -26b, -29a, -126, -144 and -144*) were significantly elevated during the pre-examination period in association with significant down-regulation of their target mRNAs (WNT4, CCM2, MAK, and FGFR1 mRNAs) two days before the examination. State anxiety assessed two months before the examination was positively and negatively correlated with miR-16 and its target WNT4 mRNA levels, respectively. Fold changes in miR-16 levels from two days before to one month after the examination were inversely correlated with those in WNT4 mRNA levels over the same time points. We also confirmed the interaction between miR-16 and WNT4 3'UTR in HEK293T cells overexpressing FLAG-tagged WNT4 3'UTR and miR-16. Thus, a distinct group of miRNAs in periheral blood may participate in the integrated response to chronic academic stress in healthy young men.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0075960</identifier><identifier>PMID: 24130753</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>3' Untranslated regions ; Adult ; Anxiety ; Blood ; Carrier Proteins - genetics ; CCM2 protein ; Enzymes ; Fibroblast growth factor receptor 1 ; Gene expression ; Genomes ; Humans ; Male ; Medical students ; Messenger RNA ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; miRNA ; mRNA ; Neurogenesis ; Peripheral blood ; Protein-Serine-Threonine Kinases - genetics ; Receptor, Fibroblast Growth Factor, Type 1 - genetics ; Science ; Stress ; Stress, Psychological - genetics ; Students ; Teaching ; Tuberculosis ; Wnt protein ; Wnt4 Protein - genetics</subject><ispartof>PloS one, 2013-10, Vol.8 (10), p.e75960-e75960</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Honda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Honda et al 2013 Honda et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-287be8c2a887d793c570656209dd0d5dae96dde47576ddb941619f4f03fdda5c3</citedby><cites>FETCH-LOGICAL-c692t-287be8c2a887d793c570656209dd0d5dae96dde47576ddb941619f4f03fdda5c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794012/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794012/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24130753$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Honda, Manami</creatorcontrib><creatorcontrib>Kuwano, Yuki</creatorcontrib><creatorcontrib>Katsuura-Kamano, Sakurako</creatorcontrib><creatorcontrib>Kamezaki, Yoshiko</creatorcontrib><creatorcontrib>Fujita, Kinuyo</creatorcontrib><creatorcontrib>Akaike, Yoko</creatorcontrib><creatorcontrib>Kano, Shizuka</creatorcontrib><creatorcontrib>Nishida, Kensei</creatorcontrib><creatorcontrib>Masuda, Kiyoshi</creatorcontrib><creatorcontrib>Rokutan, Kazuhito</creatorcontrib><title>Chronic academic stress increases a group of microRNAs in peripheral blood</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>MicroRNAs (miRNAs) play key roles in regulation of cellular processes in response to changes in environment. In this study, we examined alterations in miRNA profiles in peripheral blood from 25 male medical students two months and two days before the National Examination for Medical Practitioners. Blood obtained one month after the examination were used as baseline controls. Levels of seven miRNAs (miR-16, -20b, -26b, -29a, -126, -144 and -144*) were significantly elevated during the pre-examination period in association with significant down-regulation of their target mRNAs (WNT4, CCM2, MAK, and FGFR1 mRNAs) two days before the examination. State anxiety assessed two months before the examination was positively and negatively correlated with miR-16 and its target WNT4 mRNA levels, respectively. Fold changes in miR-16 levels from two days before to one month after the examination were inversely correlated with those in WNT4 mRNA levels over the same time points. We also confirmed the interaction between miR-16 and WNT4 3'UTR in HEK293T cells overexpressing FLAG-tagged WNT4 3'UTR and miR-16. Thus, a distinct group of miRNAs in periheral blood may participate in the integrated response to chronic academic stress in healthy young men.</description><subject>3' Untranslated regions</subject><subject>Adult</subject><subject>Anxiety</subject><subject>Blood</subject><subject>Carrier Proteins - genetics</subject><subject>CCM2 protein</subject><subject>Enzymes</subject><subject>Fibroblast growth factor receptor 1</subject><subject>Gene expression</subject><subject>Genomes</subject><subject>Humans</subject><subject>Male</subject><subject>Medical students</subject><subject>Messenger RNA</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>miRNA</subject><subject>mRNA</subject><subject>Neurogenesis</subject><subject>Peripheral blood</subject><subject>Protein-Serine-Threonine Kinases - genetics</subject><subject>Receptor, Fibroblast Growth Factor, Type 1 - genetics</subject><subject>Science</subject><subject>Stress</subject><subject>Stress, Psychological - 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In this study, we examined alterations in miRNA profiles in peripheral blood from 25 male medical students two months and two days before the National Examination for Medical Practitioners. Blood obtained one month after the examination were used as baseline controls. Levels of seven miRNAs (miR-16, -20b, -26b, -29a, -126, -144 and -144*) were significantly elevated during the pre-examination period in association with significant down-regulation of their target mRNAs (WNT4, CCM2, MAK, and FGFR1 mRNAs) two days before the examination. State anxiety assessed two months before the examination was positively and negatively correlated with miR-16 and its target WNT4 mRNA levels, respectively. Fold changes in miR-16 levels from two days before to one month after the examination were inversely correlated with those in WNT4 mRNA levels over the same time points. We also confirmed the interaction between miR-16 and WNT4 3'UTR in HEK293T cells overexpressing FLAG-tagged WNT4 3'UTR and miR-16. Thus, a distinct group of miRNAs in periheral blood may participate in the integrated response to chronic academic stress in healthy young men.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24130753</pmid><doi>10.1371/journal.pone.0075960</doi><tpages>e75960</tpages><oa>free_for_read</oa></addata></record> |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 3' Untranslated regions Adult Anxiety Blood Carrier Proteins - genetics CCM2 protein Enzymes Fibroblast growth factor receptor 1 Gene expression Genomes Humans Male Medical students Messenger RNA MicroRNA MicroRNAs MicroRNAs - genetics miRNA mRNA Neurogenesis Peripheral blood Protein-Serine-Threonine Kinases - genetics Receptor, Fibroblast Growth Factor, Type 1 - genetics Science Stress Stress, Psychological - genetics Students Teaching Tuberculosis Wnt protein Wnt4 Protein - genetics |
| title | Chronic academic stress increases a group of microRNAs in peripheral blood |
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