Curcumin suppresses gelatinase B mediated norepinephrine induced stress in H9c2 cardiomyocytes
Extracellular matrix (ECM) remodeling facilitates biomechanical signals in response to abnormal physiological conditions. This process is witnessed as one of the major effects of the stress imposed by catecholamines, such as epinephrine and norepinephrine (NE), on cardiac muscle cells. Matrix metall...
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| description | Extracellular matrix (ECM) remodeling facilitates biomechanical signals in response to abnormal physiological conditions. This process is witnessed as one of the major effects of the stress imposed by catecholamines, such as epinephrine and norepinephrine (NE), on cardiac muscle cells. Matrix metalloproteinases (MMPs) are the key proteases involved in degradation of the ECM in heart.
The present study focuses on studying the effect of curcumin on Gelatinase B (MMP-9), an ECM remodeling regulatory enzyme, in NE-induced cardiac stress. Curcumin, a bioactive polyphenol found in the spice turmeric, has been studied for its multi-fold beneficial properties. This study focuses on investigating the role of curcumin as a cardio-protectant.
H9c2 cardiomyocytes were subjected to NE and curcumin treatments to study the response in stress conditions. Effect on total collagen content was studied using Picrosirus red staining. Gelatinase B activity was assessed through Gel-Diffusion Assay and Zymographic techniques. RT-PCR, Western Blotting and Immunocytochemistry were performed to study effect on expression of gelatinase B. Further, the effect of curcumin on the localization of NF-κB, known to regulate gelatinase B, was also examined.
Curcumin suppressed the increase in the total collagen content under hypertrophic stress and was found to inhibit the in-gel and in-situ gelatinolytic activity of gelatinase B. Moreover, it was found to suppress the mRNA and protein expression of gelatinase B.
The study provides an evidence for an overall inhibitory effect of curcumin on Gelatinase B in NE-induced hypertrophic stress in H9c2 cardiomyocytes which may contribute in the prevention of ECM remodeling. |
| doi_str_mv | 10.1371/journal.pone.0076519 |
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The present study focuses on studying the effect of curcumin on Gelatinase B (MMP-9), an ECM remodeling regulatory enzyme, in NE-induced cardiac stress. Curcumin, a bioactive polyphenol found in the spice turmeric, has been studied for its multi-fold beneficial properties. This study focuses on investigating the role of curcumin as a cardio-protectant.
H9c2 cardiomyocytes were subjected to NE and curcumin treatments to study the response in stress conditions. Effect on total collagen content was studied using Picrosirus red staining. Gelatinase B activity was assessed through Gel-Diffusion Assay and Zymographic techniques. RT-PCR, Western Blotting and Immunocytochemistry were performed to study effect on expression of gelatinase B. Further, the effect of curcumin on the localization of NF-κB, known to regulate gelatinase B, was also examined.
Curcumin suppressed the increase in the total collagen content under hypertrophic stress and was found to inhibit the in-gel and in-situ gelatinolytic activity of gelatinase B. Moreover, it was found to suppress the mRNA and protein expression of gelatinase B.
The study provides an evidence for an overall inhibitory effect of curcumin on Gelatinase B in NE-induced hypertrophic stress in H9c2 cardiomyocytes which may contribute in the prevention of ECM remodeling.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0076519</identifier><identifier>PMID: 24116115</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adrenergic alpha-Agonists - pharmacology ; Animals ; Anti-Inflammatory Agents, Non-Steroidal - pharmacology ; Apoptosis ; Biomechanics ; Biotechnology ; Blotting, Western ; Cardiac muscle ; Cardiomyocytes ; Catecholamines ; Cell Line ; Cell Survival - drug effects ; Collagen ; Collagen - metabolism ; Curcumin ; Curcumin - pharmacology ; Disease ; Down-Regulation - drug effects ; Epinephrine ; Eutrophication ; Extracellular matrix ; Extracellular Matrix - metabolism ; Gel diffusion ; Gelatinase ; Gelatinase B ; Gene expression ; Gene Expression Regulation, Enzymologic - drug effects ; Heart ; Heart cells ; Heart diseases ; Heart failure ; Immunocytochemistry ; Immunohistochemistry ; Information technology ; Localization ; Matrix Metalloproteinase 9 - genetics ; Matrix Metalloproteinase 9 - metabolism ; Matrix metalloproteinases ; Models, Genetic ; mRNA ; Muscles ; Myocytes, Cardiac - cytology ; Myocytes, Cardiac - drug effects ; Myocytes, Cardiac - metabolism ; NF-κB protein ; Norepinephrine ; Norepinephrine - pharmacology ; Physiological aspects ; Physiological effects ; Polymerase chain reaction ; Proteases ; Proteins ; Rats ; Reverse Transcriptase Polymerase Chain Reaction ; Rodents ; Signal processing ; Stress ; Stress, Physiological - drug effects ; Stresses ; Western blotting</subject><ispartof>PloS one, 2013-10, Vol.8 (10), p.e76519</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Kohli et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Kohli et al 2013 Kohli et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-61da6b3dba2c7acd19f00ce9a35feae5785e866eae5edc88bfcee2ba836a84293</citedby><cites>FETCH-LOGICAL-c692t-61da6b3dba2c7acd19f00ce9a35feae5785e866eae5edc88bfcee2ba836a84293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792053/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792053/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2101,2927,23865,27923,27924,53790,53792,79471,79472</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24116115$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tsilibary, Effie C</contributor><creatorcontrib>Kohli, Shrey</creatorcontrib><creatorcontrib>Chhabra, Aastha</creatorcontrib><creatorcontrib>Jaiswal, Astha</creatorcontrib><creatorcontrib>Rustagi, Yashika</creatorcontrib><creatorcontrib>Sharma, Manish</creatorcontrib><creatorcontrib>Rani, Vibha</creatorcontrib><title>Curcumin suppresses gelatinase B mediated norepinephrine induced stress in H9c2 cardiomyocytes</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Extracellular matrix (ECM) remodeling facilitates biomechanical signals in response to abnormal physiological conditions. This process is witnessed as one of the major effects of the stress imposed by catecholamines, such as epinephrine and norepinephrine (NE), on cardiac muscle cells. Matrix metalloproteinases (MMPs) are the key proteases involved in degradation of the ECM in heart.
The present study focuses on studying the effect of curcumin on Gelatinase B (MMP-9), an ECM remodeling regulatory enzyme, in NE-induced cardiac stress. Curcumin, a bioactive polyphenol found in the spice turmeric, has been studied for its multi-fold beneficial properties. This study focuses on investigating the role of curcumin as a cardio-protectant.
H9c2 cardiomyocytes were subjected to NE and curcumin treatments to study the response in stress conditions. Effect on total collagen content was studied using Picrosirus red staining. Gelatinase B activity was assessed through Gel-Diffusion Assay and Zymographic techniques. RT-PCR, Western Blotting and Immunocytochemistry were performed to study effect on expression of gelatinase B. Further, the effect of curcumin on the localization of NF-κB, known to regulate gelatinase B, was also examined.
Curcumin suppressed the increase in the total collagen content under hypertrophic stress and was found to inhibit the in-gel and in-situ gelatinolytic activity of gelatinase B. Moreover, it was found to suppress the mRNA and protein expression of gelatinase B.
The study provides an evidence for an overall inhibitory effect of curcumin on Gelatinase B in NE-induced hypertrophic stress in H9c2 cardiomyocytes which may contribute in the prevention of ECM remodeling.</description><subject>Adrenergic alpha-Agonists - pharmacology</subject><subject>Animals</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</subject><subject>Apoptosis</subject><subject>Biomechanics</subject><subject>Biotechnology</subject><subject>Blotting, Western</subject><subject>Cardiac muscle</subject><subject>Cardiomyocytes</subject><subject>Catecholamines</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>Curcumin</subject><subject>Curcumin - pharmacology</subject><subject>Disease</subject><subject>Down-Regulation - drug effects</subject><subject>Epinephrine</subject><subject>Eutrophication</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - metabolism</subject><subject>Gel diffusion</subject><subject>Gelatinase</subject><subject>Gelatinase B</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Heart</subject><subject>Heart cells</subject><subject>Heart diseases</subject><subject>Heart failure</subject><subject>Immunocytochemistry</subject><subject>Immunohistochemistry</subject><subject>Information technology</subject><subject>Localization</subject><subject>Matrix Metalloproteinase 9 - genetics</subject><subject>Matrix Metalloproteinase 9 - metabolism</subject><subject>Matrix metalloproteinases</subject><subject>Models, Genetic</subject><subject>mRNA</subject><subject>Muscles</subject><subject>Myocytes, Cardiac - cytology</subject><subject>Myocytes, Cardiac - drug effects</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>NF-κB protein</subject><subject>Norepinephrine</subject><subject>Norepinephrine - pharmacology</subject><subject>Physiological aspects</subject><subject>Physiological effects</subject><subject>Polymerase chain reaction</subject><subject>Proteases</subject><subject>Proteins</subject><subject>Rats</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Rodents</subject><subject>Signal processing</subject><subject>Stress</subject><subject>Stress, Physiological - 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pharmacology</topic><topic>Animals</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</topic><topic>Apoptosis</topic><topic>Biomechanics</topic><topic>Biotechnology</topic><topic>Blotting, Western</topic><topic>Cardiac muscle</topic><topic>Cardiomyocytes</topic><topic>Catecholamines</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Collagen</topic><topic>Collagen - metabolism</topic><topic>Curcumin</topic><topic>Curcumin - pharmacology</topic><topic>Disease</topic><topic>Down-Regulation - drug effects</topic><topic>Epinephrine</topic><topic>Eutrophication</topic><topic>Extracellular matrix</topic><topic>Extracellular Matrix - metabolism</topic><topic>Gel diffusion</topic><topic>Gelatinase</topic><topic>Gelatinase B</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Heart</topic><topic>Heart cells</topic><topic>Heart diseases</topic><topic>Heart failure</topic><topic>Immunocytochemistry</topic><topic>Immunohistochemistry</topic><topic>Information technology</topic><topic>Localization</topic><topic>Matrix Metalloproteinase 9 - 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This process is witnessed as one of the major effects of the stress imposed by catecholamines, such as epinephrine and norepinephrine (NE), on cardiac muscle cells. Matrix metalloproteinases (MMPs) are the key proteases involved in degradation of the ECM in heart.
The present study focuses on studying the effect of curcumin on Gelatinase B (MMP-9), an ECM remodeling regulatory enzyme, in NE-induced cardiac stress. Curcumin, a bioactive polyphenol found in the spice turmeric, has been studied for its multi-fold beneficial properties. This study focuses on investigating the role of curcumin as a cardio-protectant.
H9c2 cardiomyocytes were subjected to NE and curcumin treatments to study the response in stress conditions. Effect on total collagen content was studied using Picrosirus red staining. Gelatinase B activity was assessed through Gel-Diffusion Assay and Zymographic techniques. RT-PCR, Western Blotting and Immunocytochemistry were performed to study effect on expression of gelatinase B. Further, the effect of curcumin on the localization of NF-κB, known to regulate gelatinase B, was also examined.
Curcumin suppressed the increase in the total collagen content under hypertrophic stress and was found to inhibit the in-gel and in-situ gelatinolytic activity of gelatinase B. Moreover, it was found to suppress the mRNA and protein expression of gelatinase B.
The study provides an evidence for an overall inhibitory effect of curcumin on Gelatinase B in NE-induced hypertrophic stress in H9c2 cardiomyocytes which may contribute in the prevention of ECM remodeling.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24116115</pmid><doi>10.1371/journal.pone.0076519</doi><tpages>e76519</tpages><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1440074199 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adrenergic alpha-Agonists - pharmacology Animals Anti-Inflammatory Agents, Non-Steroidal - pharmacology Apoptosis Biomechanics Biotechnology Blotting, Western Cardiac muscle Cardiomyocytes Catecholamines Cell Line Cell Survival - drug effects Collagen Collagen - metabolism Curcumin Curcumin - pharmacology Disease Down-Regulation - drug effects Epinephrine Eutrophication Extracellular matrix Extracellular Matrix - metabolism Gel diffusion Gelatinase Gelatinase B Gene expression Gene Expression Regulation, Enzymologic - drug effects Heart Heart cells Heart diseases Heart failure Immunocytochemistry Immunohistochemistry Information technology Localization Matrix Metalloproteinase 9 - genetics Matrix Metalloproteinase 9 - metabolism Matrix metalloproteinases Models, Genetic mRNA Muscles Myocytes, Cardiac - cytology Myocytes, Cardiac - drug effects Myocytes, Cardiac - metabolism NF-κB protein Norepinephrine Norepinephrine - pharmacology Physiological aspects Physiological effects Polymerase chain reaction Proteases Proteins Rats Reverse Transcriptase Polymerase Chain Reaction Rodents Signal processing Stress Stress, Physiological - drug effects Stresses Western blotting |
| title | Curcumin suppresses gelatinase B mediated norepinephrine induced stress in H9c2 cardiomyocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T12%3A47%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Curcumin%20suppresses%20gelatinase%20B%20mediated%20norepinephrine%20induced%20stress%20in%20H9c2%20cardiomyocytes&rft.jtitle=PloS%20one&rft.au=Kohli,%20Shrey&rft.date=2013-10-07&rft.volume=8&rft.issue=10&rft.spage=e76519&rft.pages=e76519-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0076519&rft_dat=%3Cgale_plos_%3EA478387512%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1440074199&rft_id=info:pmid/24116115&rft_galeid=A478387512&rft_doaj_id=oai_doaj_org_article_85faf389b9864b8a983754c291dbfd8b&rfr_iscdi=true |