Application of Genotyping-by-Sequencing on Semiconductor Sequencing Platforms: A Comparison of Genetic and Reference-Based Marker Ordering in Barley

The rapid development of next-generation sequencing platforms has enabled the use of sequencing for routine genotyping across a range of genetics studies and breeding applications. Genotyping-by-sequencing (GBS), a low-cost, reduced representation sequencing method, is becoming a common approach for...

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Veröffentlicht in:	PloS one 2013-10, Vol.8 (10), p.e76925-e76925
Hauptverfasser:	Mascher, Martin, Wu, Shuangye, Amand, Paul St, Stein, Nils, Poland, Jesse, Candela, Hector
Format:	Artikel
Sprache:	eng
Schlagworte:	Adapters Agriculture Bar codes Barley Base Sequence Bioinformatics Breeding chromosome mapping Chromosome Mapping - instrumentation Chromosome Mapping - methods Chromosomes, Plant - chemistry Comparative analysis Computer applications Data processing Deoxyribonucleic acid DNA Enzymes Filtration Gene Library Gene mapping Gene sequencing Genetic Markers Genetics genome assembly Genome, Plant Genomes Genomics Genotype Genotyping Germplasm High-Throughput Nucleotide Sequencing Hordeum - classification Hordeum - genetics Hordeum vulgare inbred lines Inbreeding Library collections Low cost Mapping Molecular Sequence Data Molecular Typing - instrumentation Molecular Typing - methods Mutation Parameter estimation Parents Pipelines Platforms Polymorphism, Single Nucleotide Semiconductors Sensors Sequence Alignment Single-nucleotide polymorphism Triticum Triticum aestivum Wheat
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