Evaluation of four endogenous reference genes and their real-time PCR assays for common wheat quantification in GMOs detection

Proper selection of endogenous reference genes and their real-time PCR assays is quite important in genetically modified organisms (GMOs) detection. To find a suitable endogenous reference gene and its real-time PCR assay for common wheat (Triticum aestivum L.) DNA content or copy number quantificat...

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Veröffentlicht in:	PloS one 2013-09, Vol.8 (9), p.e75850-e75850
Hauptverfasser:	Huang, Huali, Cheng, Fang, Wang, Ruoan, Zhang, Dabing, Yang, Litao
Format:	Artikel
Sprache:	eng
Schlagworte:	Assaying Biotechnology industry Copy number Deoxyribonucleic acid DNA DNA Primers - genetics Food safety Genes Genes, Plant - genetics Genetic modification Genetic Variation Genetically altered foods Genetically engineered organisms Genetically modified organisms Genomes Life sciences Organisms Plant Proteins - genetics Plants, Genetically Modified - genetics Polymerase chain reaction Polymorphism, Single Nucleotide - genetics Qualitative analysis Real time Real-Time Polymerase Chain Reaction Reproducibility of Results Single-nucleotide polymorphism Starch Synthase - genetics Triticum - genetics Triticum aestivum Triticum durum Wheat
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