Body sodium overload modulates the firing rate and fos immunoreactivity of serotonergic cells of dorsal raphe nucleus
In order to determine whether serotonergic (5HT) dorsal raphe nucleus (DRN) cells are involved in body sodium status regulation, the effect of a s.c. infusion of either 2 M or 0.15 M NaCl on 5HT DRN neuron firing was studied using single unit extracellular recordings. In separate groups of 2 M and 0...
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| description | In order to determine whether serotonergic (5HT) dorsal raphe nucleus (DRN) cells are involved in body sodium status regulation, the effect of a s.c. infusion of either 2 M or 0.15 M NaCl on 5HT DRN neuron firing was studied using single unit extracellular recordings. In separate groups of 2 M and 0.15 M NaCl-infused rats, water intake, oxytocin (OT) plasma concentration, urine and plasma sodium and protein concentrations were also measured. Also, to determine the involvement of particular brain nuclei and neurochemical systems in body sodium overload (SO), animals from both groups were perfused for brain immunohistochemical detection of Fos, Fos-OT and Fos-5HT expression. SO produced a significant increase in serotonergic DRN neuron firing rate compared to baseline and 0.15 M NaCl-infused rats. As expected, 2 M NaCl s.c. infusion also induced a significant increase of water intake, diuresis and natriuresis, plasma sodium concentration and osmolality, even though plasma volume did not increase as indicated by changes in plasma protein concentration. The distribution of neurons along the forebrain and brainstem expressing Fos after SO showed the participation of the lamina terminalis, extended amygdala, supraoptic and paraventricular hypothalamic nuclei in the neural network that controls osmoregulatory responses. Both Fos-OT immunoreactive and plasma OT concentration increased after s.c. hypertonic sodium infusion. Finally, matching the "in vivo" electrophysiological study, SO doubled the number of Fos-5HT immunolabeled cells within the DRN. In summary, the results characterize the behavioral, renal and endocrine responses after body sodium overload without volume expansion and specify the cerebral nuclei that participate at different CNS levels in the control of these responses. The electrophysiological approach also allows us to determine in an "in vivo" model that DRN 5HT neurons increase their firing frequency during an increase in systemic sodium concentration and osmolality, possibly to modulate sodium and water intake/excretion and avoid extracellular volume expansion. |
| doi_str_mv | 10.1371/journal.pone.0074689 |
| format | Article |
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In separate groups of 2 M and 0.15 M NaCl-infused rats, water intake, oxytocin (OT) plasma concentration, urine and plasma sodium and protein concentrations were also measured. Also, to determine the involvement of particular brain nuclei and neurochemical systems in body sodium overload (SO), animals from both groups were perfused for brain immunohistochemical detection of Fos, Fos-OT and Fos-5HT expression. SO produced a significant increase in serotonergic DRN neuron firing rate compared to baseline and 0.15 M NaCl-infused rats. As expected, 2 M NaCl s.c. infusion also induced a significant increase of water intake, diuresis and natriuresis, plasma sodium concentration and osmolality, even though plasma volume did not increase as indicated by changes in plasma protein concentration. The distribution of neurons along the forebrain and brainstem expressing Fos after SO showed the participation of the lamina terminalis, extended amygdala, supraoptic and paraventricular hypothalamic nuclei in the neural network that controls osmoregulatory responses. Both Fos-OT immunoreactive and plasma OT concentration increased after s.c. hypertonic sodium infusion. Finally, matching the "in vivo" electrophysiological study, SO doubled the number of Fos-5HT immunolabeled cells within the DRN. In summary, the results characterize the behavioral, renal and endocrine responses after body sodium overload without volume expansion and specify the cerebral nuclei that participate at different CNS levels in the control of these responses. The electrophysiological approach also allows us to determine in an "in vivo" model that DRN 5HT neurons increase their firing frequency during an increase in systemic sodium concentration and osmolality, possibly to modulate sodium and water intake/excretion and avoid extracellular volume expansion.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0074689</identifier><identifier>PMID: 24073217</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amygdala ; Animals ; Artificial neural networks ; Behavior ; Blood proteins ; Brain ; Brain stem ; Central nervous system ; Diuresis ; Dorsal raphe nucleus ; Electrophysiological recording ; Excretion ; Firing ; Firing rate ; Forebrain ; Hypernatremia - physiopathology ; Hypothalamus ; Immunoreactivity ; In vivo methods and tests ; Infusion ; Kidney - drug effects ; Kidney - immunology ; Kidney - metabolism ; Kidney Function Tests ; Kidneys ; Laboratory animals ; Male ; Metabolism ; Neural networks ; Neurons ; Nuclei (cytology) ; Osmoregulation ; Oxytocin ; Oxytocin - immunology ; Oxytocin - pharmacology ; Physiology ; Plasma ; Proto-Oncogene Proteins c-fos - immunology ; Proto-Oncogene Proteins c-fos - metabolism ; Raphe nuclei ; Raphe Nuclei - drug effects ; Raphe Nuclei - immunology ; Raphe Nuclei - metabolism ; Rats ; Rats, Wistar ; Regulation ; Rodents ; Serotonin ; Serotonin - immunology ; Serotonin - pharmacology ; Serotonin Receptor Agonists - immunology ; Serotonin Receptor Agonists - pharmacology ; Sodium ; Sodium chloride ; Sodium, Dietary - administration & dosage ; Urine ; Water intake ; Water intakes</subject><ispartof>PloS one, 2013-09, Vol.8 (9), p.e74689-e74689</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Godino et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Godino et al 2013 Godino et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-1f4d9ab726029a0c7edbdc51f6b7dbc7e370524fa3f322ef7508e955ffa996923</citedby><cites>FETCH-LOGICAL-c692t-1f4d9ab726029a0c7edbdc51f6b7dbc7e370524fa3f322ef7508e955ffa996923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3779203/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3779203/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24073217$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tache, Yvette</contributor><creatorcontrib>Godino, Andrea</creatorcontrib><creatorcontrib>Pitra, Soledad</creatorcontrib><creatorcontrib>Carrer, Hugo F</creatorcontrib><creatorcontrib>Vivas, Laura</creatorcontrib><title>Body sodium overload modulates the firing rate and fos immunoreactivity of serotonergic cells of dorsal raphe nucleus</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In order to determine whether serotonergic (5HT) dorsal raphe nucleus (DRN) cells are involved in body sodium status regulation, the effect of a s.c. infusion of either 2 M or 0.15 M NaCl on 5HT DRN neuron firing was studied using single unit extracellular recordings. In separate groups of 2 M and 0.15 M NaCl-infused rats, water intake, oxytocin (OT) plasma concentration, urine and plasma sodium and protein concentrations were also measured. Also, to determine the involvement of particular brain nuclei and neurochemical systems in body sodium overload (SO), animals from both groups were perfused for brain immunohistochemical detection of Fos, Fos-OT and Fos-5HT expression. SO produced a significant increase in serotonergic DRN neuron firing rate compared to baseline and 0.15 M NaCl-infused rats. As expected, 2 M NaCl s.c. infusion also induced a significant increase of water intake, diuresis and natriuresis, plasma sodium concentration and osmolality, even though plasma volume did not increase as indicated by changes in plasma protein concentration. The distribution of neurons along the forebrain and brainstem expressing Fos after SO showed the participation of the lamina terminalis, extended amygdala, supraoptic and paraventricular hypothalamic nuclei in the neural network that controls osmoregulatory responses. Both Fos-OT immunoreactive and plasma OT concentration increased after s.c. hypertonic sodium infusion. Finally, matching the "in vivo" electrophysiological study, SO doubled the number of Fos-5HT immunolabeled cells within the DRN. In summary, the results characterize the behavioral, renal and endocrine responses after body sodium overload without volume expansion and specify the cerebral nuclei that participate at different CNS levels in the control of these responses. The electrophysiological approach also allows us to determine in an "in vivo" model that DRN 5HT neurons increase their firing frequency during an increase in systemic sodium concentration and osmolality, possibly to modulate sodium and water intake/excretion and avoid extracellular volume expansion.</description><subject>Amygdala</subject><subject>Animals</subject><subject>Artificial neural networks</subject><subject>Behavior</subject><subject>Blood proteins</subject><subject>Brain</subject><subject>Brain stem</subject><subject>Central nervous system</subject><subject>Diuresis</subject><subject>Dorsal raphe nucleus</subject><subject>Electrophysiological recording</subject><subject>Excretion</subject><subject>Firing</subject><subject>Firing rate</subject><subject>Forebrain</subject><subject>Hypernatremia - physiopathology</subject><subject>Hypothalamus</subject><subject>Immunoreactivity</subject><subject>In vivo methods and tests</subject><subject>Infusion</subject><subject>Kidney - drug effects</subject><subject>Kidney - immunology</subject><subject>Kidney - metabolism</subject><subject>Kidney Function Tests</subject><subject>Kidneys</subject><subject>Laboratory animals</subject><subject>Male</subject><subject>Metabolism</subject><subject>Neural networks</subject><subject>Neurons</subject><subject>Nuclei (cytology)</subject><subject>Osmoregulation</subject><subject>Oxytocin</subject><subject>Oxytocin - immunology</subject><subject>Oxytocin - pharmacology</subject><subject>Physiology</subject><subject>Plasma</subject><subject>Proto-Oncogene Proteins c-fos - immunology</subject><subject>Proto-Oncogene Proteins c-fos - metabolism</subject><subject>Raphe nuclei</subject><subject>Raphe Nuclei - drug effects</subject><subject>Raphe Nuclei - immunology</subject><subject>Raphe Nuclei - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Regulation</subject><subject>Rodents</subject><subject>Serotonin</subject><subject>Serotonin - immunology</subject><subject>Serotonin - pharmacology</subject><subject>Serotonin Receptor Agonists - immunology</subject><subject>Serotonin Receptor Agonists - pharmacology</subject><subject>Sodium</subject><subject>Sodium chloride</subject><subject>Sodium, Dietary - administration & dosage</subject><subject>Urine</subject><subject>Water intake</subject><subject>Water intakes</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1trFDEUxwdRbK1-A9GAIPqway4zk8mLUIuXhULB22vITJLZlMxkm8vifnuz3WnZkT5IHpKc_P7_nJxwiuIlgktEKPpw7ZIfhV1u3KiWENKybtij4hQxghc1huTx0fqkeBbCNYQVaer6aXGCS0gJRvS0SJ-c3IHgpEkDcFvlrRMSDE4mK6IKIK4V0MabsQc-B4AYJdAuADMMaXReiS6arYk74DQIyruYs_G96UCnrA37qHQ-CJvVm2w1ps6qFJ4XT7SwQb2Y5rPi15fPPy--LS6vvq4uzi8XXc1wXCBdSiZaimuImYAdVbKVXYV03VLZ5i2hsMKlFkQTjJWmFWwUqyqtBWPZgZwVrw--G-sCnyoWOCpJWWLCEMrE6kBIJ675xptB-B13wvDbgPM9Fz6anDUXBFPdItS2OS_KMFOVwIJVsm0r0gqdvT5Ot6V2ULJTY_TCzkznJ6NZ895tOaHZDpJs8G4y8O4mqRD5YMK-kGJULt3mTSvKmpJm9M0_6MOvm6he5AeYUbt8b7c35eclbVBTQ1ZlavkAlYdUg-nyh2qT4zPB-5kgM1H9ib1IIfDVj-__z179nrNvj9i1Ejaug7MpGjeGOVgewM67ELzS90VGkO-7464afN8dfOqOLHt1_EH3ort2IH8BPF8Mrw</recordid><startdate>20130920</startdate><enddate>20130920</enddate><creator>Godino, Andrea</creator><creator>Pitra, Soledad</creator><creator>Carrer, Hugo F</creator><creator>Vivas, Laura</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130920</creationdate><title>Body sodium overload modulates the firing rate and fos immunoreactivity of serotonergic cells of dorsal raphe nucleus</title><author>Godino, Andrea ; Pitra, Soledad ; Carrer, Hugo F ; Vivas, Laura</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-1f4d9ab726029a0c7edbdc51f6b7dbc7e370524fa3f322ef7508e955ffa996923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amygdala</topic><topic>Animals</topic><topic>Artificial neural networks</topic><topic>Behavior</topic><topic>Blood proteins</topic><topic>Brain</topic><topic>Brain stem</topic><topic>Central nervous system</topic><topic>Diuresis</topic><topic>Dorsal raphe nucleus</topic><topic>Electrophysiological recording</topic><topic>Excretion</topic><topic>Firing</topic><topic>Firing rate</topic><topic>Forebrain</topic><topic>Hypernatremia - physiopathology</topic><topic>Hypothalamus</topic><topic>Immunoreactivity</topic><topic>In vivo methods and tests</topic><topic>Infusion</topic><topic>Kidney - drug effects</topic><topic>Kidney - immunology</topic><topic>Kidney - metabolism</topic><topic>Kidney Function Tests</topic><topic>Kidneys</topic><topic>Laboratory animals</topic><topic>Male</topic><topic>Metabolism</topic><topic>Neural networks</topic><topic>Neurons</topic><topic>Nuclei (cytology)</topic><topic>Osmoregulation</topic><topic>Oxytocin</topic><topic>Oxytocin - immunology</topic><topic>Oxytocin - pharmacology</topic><topic>Physiology</topic><topic>Plasma</topic><topic>Proto-Oncogene Proteins c-fos - immunology</topic><topic>Proto-Oncogene Proteins c-fos - metabolism</topic><topic>Raphe nuclei</topic><topic>Raphe Nuclei - drug effects</topic><topic>Raphe Nuclei - immunology</topic><topic>Raphe Nuclei - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Regulation</topic><topic>Rodents</topic><topic>Serotonin</topic><topic>Serotonin - immunology</topic><topic>Serotonin - pharmacology</topic><topic>Serotonin Receptor Agonists - immunology</topic><topic>Serotonin Receptor Agonists - pharmacology</topic><topic>Sodium</topic><topic>Sodium chloride</topic><topic>Sodium, Dietary - administration & dosage</topic><topic>Urine</topic><topic>Water intake</topic><topic>Water intakes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Godino, Andrea</creatorcontrib><creatorcontrib>Pitra, Soledad</creatorcontrib><creatorcontrib>Carrer, Hugo F</creatorcontrib><creatorcontrib>Vivas, Laura</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Godino, Andrea</au><au>Pitra, Soledad</au><au>Carrer, Hugo F</au><au>Vivas, Laura</au><au>Tache, Yvette</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Body sodium overload modulates the firing rate and fos immunoreactivity of serotonergic cells of dorsal raphe nucleus</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-09-20</date><risdate>2013</risdate><volume>8</volume><issue>9</issue><spage>e74689</spage><epage>e74689</epage><pages>e74689-e74689</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In order to determine whether serotonergic (5HT) dorsal raphe nucleus (DRN) cells are involved in body sodium status regulation, the effect of a s.c. infusion of either 2 M or 0.15 M NaCl on 5HT DRN neuron firing was studied using single unit extracellular recordings. In separate groups of 2 M and 0.15 M NaCl-infused rats, water intake, oxytocin (OT) plasma concentration, urine and plasma sodium and protein concentrations were also measured. Also, to determine the involvement of particular brain nuclei and neurochemical systems in body sodium overload (SO), animals from both groups were perfused for brain immunohistochemical detection of Fos, Fos-OT and Fos-5HT expression. SO produced a significant increase in serotonergic DRN neuron firing rate compared to baseline and 0.15 M NaCl-infused rats. As expected, 2 M NaCl s.c. infusion also induced a significant increase of water intake, diuresis and natriuresis, plasma sodium concentration and osmolality, even though plasma volume did not increase as indicated by changes in plasma protein concentration. The distribution of neurons along the forebrain and brainstem expressing Fos after SO showed the participation of the lamina terminalis, extended amygdala, supraoptic and paraventricular hypothalamic nuclei in the neural network that controls osmoregulatory responses. Both Fos-OT immunoreactive and plasma OT concentration increased after s.c. hypertonic sodium infusion. Finally, matching the "in vivo" electrophysiological study, SO doubled the number of Fos-5HT immunolabeled cells within the DRN. In summary, the results characterize the behavioral, renal and endocrine responses after body sodium overload without volume expansion and specify the cerebral nuclei that participate at different CNS levels in the control of these responses. The electrophysiological approach also allows us to determine in an "in vivo" model that DRN 5HT neurons increase their firing frequency during an increase in systemic sodium concentration and osmolality, possibly to modulate sodium and water intake/excretion and avoid extracellular volume expansion.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24073217</pmid><doi>10.1371/journal.pone.0074689</doi><tpages>e74689</tpages><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1434423911 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Amygdala Animals Artificial neural networks Behavior Blood proteins Brain Brain stem Central nervous system Diuresis Dorsal raphe nucleus Electrophysiological recording Excretion Firing Firing rate Forebrain Hypernatremia - physiopathology Hypothalamus Immunoreactivity In vivo methods and tests Infusion Kidney - drug effects Kidney - immunology Kidney - metabolism Kidney Function Tests Kidneys Laboratory animals Male Metabolism Neural networks Neurons Nuclei (cytology) Osmoregulation Oxytocin Oxytocin - immunology Oxytocin - pharmacology Physiology Plasma Proto-Oncogene Proteins c-fos - immunology Proto-Oncogene Proteins c-fos - metabolism Raphe nuclei Raphe Nuclei - drug effects Raphe Nuclei - immunology Raphe Nuclei - metabolism Rats Rats, Wistar Regulation Rodents Serotonin Serotonin - immunology Serotonin - pharmacology Serotonin Receptor Agonists - immunology Serotonin Receptor Agonists - pharmacology Sodium Sodium chloride Sodium, Dietary - administration & dosage Urine Water intake Water intakes |
| title | Body sodium overload modulates the firing rate and fos immunoreactivity of serotonergic cells of dorsal raphe nucleus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T20%3A01%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Body%20sodium%20overload%20modulates%20the%20firing%20rate%20and%20fos%20immunoreactivity%20of%20serotonergic%20cells%20of%20dorsal%20raphe%20nucleus&rft.jtitle=PloS%20one&rft.au=Godino,%20Andrea&rft.date=2013-09-20&rft.volume=8&rft.issue=9&rft.spage=e74689&rft.epage=e74689&rft.pages=e74689-e74689&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0074689&rft_dat=%3Cgale_plos_%3EA478186095%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1434423911&rft_id=info:pmid/24073217&rft_galeid=A478186095&rft_doaj_id=oai_doaj_org_article_a327fb11bb1f47929e5a2a95dbb53baf&rfr_iscdi=true |