Export of extracellular polysaccharides modulates adherence of the Cyanobacterium synechocystis
The field of cyanobacterial biofuel production is advancing rapidly, yet we know little of the basic biology of these organisms outside of their photosynthetic pathways. We aimed to gain a greater understanding of how the cyanobacterium Synechocystis PCC 6803 (Synechocystis, hereafter) modulates its...
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| description | The field of cyanobacterial biofuel production is advancing rapidly, yet we know little of the basic biology of these organisms outside of their photosynthetic pathways. We aimed to gain a greater understanding of how the cyanobacterium Synechocystis PCC 6803 (Synechocystis, hereafter) modulates its cell surface. Such understanding will allow for the creation of mutants that autoflocculate in a regulated way, thus avoiding energy intensive centrifugation in the creation of biofuels. We constructed mutant strains lacking genes predicted to function in carbohydrate transport or synthesis. Strains with gene deletions of slr0977 (predicted to encode a permease component of an ABC transporter), slr0982 (predicted to encode an ATP binding component of an ABC transporter) and slr1610 (predicted to encode a methyltransferase) demonstrated flocculent phenotypes and increased adherence to glass. Upon bioinformatic inspection, the gene products of slr0977, slr0982, and slr1610 appear to function in O-antigen (OAg) transport and synthesis. However, the analysis provided here demonstrated no differences between OAg purified from wild-type and mutants. However, exopolysaccharides (EPS) purified from mutants were altered in composition when compared to wild-type. Our data suggest that there are multiple means to modulate the cell surface of Synechocystis by disrupting different combinations of ABC transporters and/or glycosyl transferases. Further understanding of these mechanisms may allow for the development of industrially and ecologically useful strains of cyanobacteria. Additionally, these data imply that many cyanobacterial gene products may possess as-yet undiscovered functions, and are meritorious of further study. |
| doi_str_mv | 10.1371/journal.pone.0074514 |
| format | Article |
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We aimed to gain a greater understanding of how the cyanobacterium Synechocystis PCC 6803 (Synechocystis, hereafter) modulates its cell surface. Such understanding will allow for the creation of mutants that autoflocculate in a regulated way, thus avoiding energy intensive centrifugation in the creation of biofuels. We constructed mutant strains lacking genes predicted to function in carbohydrate transport or synthesis. Strains with gene deletions of slr0977 (predicted to encode a permease component of an ABC transporter), slr0982 (predicted to encode an ATP binding component of an ABC transporter) and slr1610 (predicted to encode a methyltransferase) demonstrated flocculent phenotypes and increased adherence to glass. Upon bioinformatic inspection, the gene products of slr0977, slr0982, and slr1610 appear to function in O-antigen (OAg) transport and synthesis. However, the analysis provided here demonstrated no differences between OAg purified from wild-type and mutants. However, exopolysaccharides (EPS) purified from mutants were altered in composition when compared to wild-type. Our data suggest that there are multiple means to modulate the cell surface of Synechocystis by disrupting different combinations of ABC transporters and/or glycosyl transferases. Further understanding of these mechanisms may allow for the development of industrially and ecologically useful strains of cyanobacteria. Additionally, these data imply that many cyanobacterial gene products may possess as-yet undiscovered functions, and are meritorious of further study.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0074514</identifier><identifier>PMID: 24040267</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>ABC transporter ; ATP-Binding Cassette Transporters - deficiency ; ATP-Binding Cassette Transporters - genetics ; Bacterial Adhesion ; Biodiesel fuels ; Biofuels ; Biological Transport ; Biomass ; Biomass energy ; Carbohydrates ; Cell surface ; Centrifugation ; Cloning ; Cyanobacteria ; E coli ; Exopolysaccharides ; Exports ; Gene Expression Regulation, Bacterial ; Genes ; Genes, Bacterial ; Genetic engineering ; Genomes ; Glass ; Gram-negative bacteria ; Industrial Microbiology ; Infectious diseases ; Inspection ; International trade ; Laboratories ; Metabolic Engineering ; Methyltransferase ; Methyltransferases - deficiency ; Methyltransferases - genetics ; Multigene Family ; Mutagenesis ; Mutants ; O Antigens - genetics ; O Antigens - metabolism ; Permease ; Photosynthesis ; Polysaccharides ; Proteins ; Saccharides ; Strains (organisms) ; Synechocystis ; Synechocystis - genetics ; Synechocystis - metabolism ; Transferases ; Transport</subject><ispartof>PloS one, 2013-09, Vol.8 (9), p.e74514-e74514</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Fisher et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Fisher et al 2013 Fisher et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c785t-b06ee231f3182cb782e8d2abd71dc62371851a802f2996f909352ade4bfc84363</citedby><cites>FETCH-LOGICAL-c785t-b06ee231f3182cb782e8d2abd71dc62371851a802f2996f909352ade4bfc84363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769361/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769361/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24040267$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1211509$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><contributor>Neilan, Brett</contributor><creatorcontrib>Fisher, Michael L</creatorcontrib><creatorcontrib>Allen, Rebecca</creatorcontrib><creatorcontrib>Luo, Yingqin</creatorcontrib><creatorcontrib>Curtiss, 3rd, Roy</creatorcontrib><title>Export of extracellular polysaccharides modulates adherence of the Cyanobacterium synechocystis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The field of cyanobacterial biofuel production is advancing rapidly, yet we know little of the basic biology of these organisms outside of their photosynthetic pathways. We aimed to gain a greater understanding of how the cyanobacterium Synechocystis PCC 6803 (Synechocystis, hereafter) modulates its cell surface. Such understanding will allow for the creation of mutants that autoflocculate in a regulated way, thus avoiding energy intensive centrifugation in the creation of biofuels. We constructed mutant strains lacking genes predicted to function in carbohydrate transport or synthesis. Strains with gene deletions of slr0977 (predicted to encode a permease component of an ABC transporter), slr0982 (predicted to encode an ATP binding component of an ABC transporter) and slr1610 (predicted to encode a methyltransferase) demonstrated flocculent phenotypes and increased adherence to glass. Upon bioinformatic inspection, the gene products of slr0977, slr0982, and slr1610 appear to function in O-antigen (OAg) transport and synthesis. However, the analysis provided here demonstrated no differences between OAg purified from wild-type and mutants. However, exopolysaccharides (EPS) purified from mutants were altered in composition when compared to wild-type. Our data suggest that there are multiple means to modulate the cell surface of Synechocystis by disrupting different combinations of ABC transporters and/or glycosyl transferases. Further understanding of these mechanisms may allow for the development of industrially and ecologically useful strains of cyanobacteria. Additionally, these data imply that many cyanobacterial gene products may possess as-yet undiscovered functions, and are meritorious of further study.</description><subject>ABC transporter</subject><subject>ATP-Binding Cassette Transporters - deficiency</subject><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>Bacterial Adhesion</subject><subject>Biodiesel fuels</subject><subject>Biofuels</subject><subject>Biological Transport</subject><subject>Biomass</subject><subject>Biomass energy</subject><subject>Carbohydrates</subject><subject>Cell surface</subject><subject>Centrifugation</subject><subject>Cloning</subject><subject>Cyanobacteria</subject><subject>E coli</subject><subject>Exopolysaccharides</subject><subject>Exports</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Genetic engineering</subject><subject>Genomes</subject><subject>Glass</subject><subject>Gram-negative bacteria</subject><subject>Industrial Microbiology</subject><subject>Infectious diseases</subject><subject>Inspection</subject><subject>International trade</subject><subject>Laboratories</subject><subject>Metabolic Engineering</subject><subject>Methyltransferase</subject><subject>Methyltransferases - deficiency</subject><subject>Methyltransferases - genetics</subject><subject>Multigene Family</subject><subject>Mutagenesis</subject><subject>Mutants</subject><subject>O Antigens - genetics</subject><subject>O Antigens - metabolism</subject><subject>Permease</subject><subject>Photosynthesis</subject><subject>Polysaccharides</subject><subject>Proteins</subject><subject>Saccharides</subject><subject>Strains (organisms)</subject><subject>Synechocystis</subject><subject>Synechocystis - genetics</subject><subject>Synechocystis - metabolism</subject><subject>Transferases</subject><subject>Transport</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk02P0zAQhiMEYpfCP0BQgYTg0OKPxE4uSKtqgUorrcTX1Zo4k8ZVGhfbQdt_j7PNrhq0B-SDrckzrzPveJLkJSVLyiX9uLW966Bd7m2HS0JkmtH0UXJOC84WghH--OR8ljzzfktIxnMhniZnLCUpYUKeJ-ryZm9dmNt6jjfBgca27Vtw871tDx60bsCZCv18Z6sYD_EEVYMOO41DUmhwvjpAZ0vQAZ3pd3N_6FA3Vh98MP558qSG1uOLcZ8lPz9f_lh9XVxdf1mvLq4WWuZZWJREIDJOa05zpkuZM8wrBmUlaaUFi_XmGYWcsJoVhagLUvCMQYVpWes85YLPktdH3X1rvRq98YqmnMZahZSRWB-JysJW7Z3ZgTsoC0bdBqzbKHDB6BZVRSmkVNI64zLuDISkRKMsoaREcIhan8bb-nKHlcYuWtdORKdfOtOojf2juBQFFzQKvDkK2GiS8tqEaJm2XXQuKMoozWKFs-T9eIuzv3v0Qe2MHxoEHdr-tjjOhCgKHtG3_6APWzBSG4hVmq62Q8cHUXWRypzzjIvByuUDVFwV7kz8R6xNjE8SPkwSIhPiY9pA771af__2_-z1ryn77oRtENrQeNv2wdjOT8H0CGpnvXdY33eCEjUMy50bahgWNQ5LTHt12sX7pLvp4H8BbRsO2w</recordid><startdate>20130910</startdate><enddate>20130910</enddate><creator>Fisher, Michael L</creator><creator>Allen, Rebecca</creator><creator>Luo, Yingqin</creator><creator>Curtiss, 3rd, Roy</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130910</creationdate><title>Export of extracellular polysaccharides modulates adherence of the Cyanobacterium synechocystis</title><author>Fisher, Michael L ; Allen, Rebecca ; Luo, Yingqin ; Curtiss, 3rd, Roy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c785t-b06ee231f3182cb782e8d2abd71dc62371851a802f2996f909352ade4bfc84363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>ABC transporter</topic><topic>ATP-Binding Cassette Transporters - 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However, exopolysaccharides (EPS) purified from mutants were altered in composition when compared to wild-type. Our data suggest that there are multiple means to modulate the cell surface of Synechocystis by disrupting different combinations of ABC transporters and/or glycosyl transferases. Further understanding of these mechanisms may allow for the development of industrially and ecologically useful strains of cyanobacteria. Additionally, these data imply that many cyanobacterial gene products may possess as-yet undiscovered functions, and are meritorious of further study.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24040267</pmid><doi>10.1371/journal.pone.0074514</doi><tpages>e74514</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-09, Vol.8 (9), p.e74514-e74514 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1431404677 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | ABC transporter ATP-Binding Cassette Transporters - deficiency ATP-Binding Cassette Transporters - genetics Bacterial Adhesion Biodiesel fuels Biofuels Biological Transport Biomass Biomass energy Carbohydrates Cell surface Centrifugation Cloning Cyanobacteria E coli Exopolysaccharides Exports Gene Expression Regulation, Bacterial Genes Genes, Bacterial Genetic engineering Genomes Glass Gram-negative bacteria Industrial Microbiology Infectious diseases Inspection International trade Laboratories Metabolic Engineering Methyltransferase Methyltransferases - deficiency Methyltransferases - genetics Multigene Family Mutagenesis Mutants O Antigens - genetics O Antigens - metabolism Permease Photosynthesis Polysaccharides Proteins Saccharides Strains (organisms) Synechocystis Synechocystis - genetics Synechocystis - metabolism Transferases Transport |
| title | Export of extracellular polysaccharides modulates adherence of the Cyanobacterium synechocystis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T15%3A22%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Export%20of%20extracellular%20polysaccharides%20modulates%20adherence%20of%20the%20Cyanobacterium%20synechocystis&rft.jtitle=PloS%20one&rft.au=Fisher,%20Michael%20L&rft.date=2013-09-10&rft.volume=8&rft.issue=9&rft.spage=e74514&rft.epage=e74514&rft.pages=e74514-e74514&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0074514&rft_dat=%3Cgale_plos_%3EA478335366%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1431404677&rft_id=info:pmid/24040267&rft_galeid=A478335366&rft_doaj_id=oai_doaj_org_article_d11a4171f5374172a6710ce7bab1063a&rfr_iscdi=true |