AFLP polymorphisms allow high resolution genetic analysis of American Tegumentary Leishmaniasis agents circulating in Panama and other members of the Leishmania genus
American Tegumentary Leishmaniasis is caused by parasites of the genus Leishmania, and causes significant health problems throughout the Americas. In Panama, Leishmania parasites are endemic, causing thousands of new cases every year, mostly of the cutaneous form. In the last years, the burden of th...
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| description | American Tegumentary Leishmaniasis is caused by parasites of the genus Leishmania, and causes significant health problems throughout the Americas. In Panama, Leishmania parasites are endemic, causing thousands of new cases every year, mostly of the cutaneous form. In the last years, the burden of the disease has increased, coincident with increasing disturbances in its natural sylvatic environments. The study of genetic variation in parasites is important for a better understanding of the biology, population genetics, and ultimately the evolution and epidemiology of these organisms. Very few attempts have been made to characterize genetic polymorphisms of parasites isolated from Panamanian patients of cutaneous leishmaniasis. Here we present data on the genetic variability of local isolates of Leishmania, as well as specimens from several other species, by means of Amplified Fragment Length Polymorphisms (AFLP), a technique seldom used to study genetic makeup of parasites. We demonstrate that this technique allows detection of very high levels of genetic variability in local isolates of Leishmania panamensis in a highly reproducible manner. The analysis of AFLP fingerprints generated by unique selective primer combinations in L. panamensis suggests a predominant clonal mode of reproduction. Using fluorescently labeled primers, many taxon-specific fragments were identified which may show potential as species diagnostic fragments. The AFLP permitted a high resolution genetic analysis of the Leishmania genus, clearly separating certain groups among L. panamensis specimens and highly related species such as L. panamensis and L. guyanensis. The phylogenetic networks reconstructed from our AFLP data are congruent with established taxonomy for the genus Leishmania, even when using single selective primer combinations. Results of this study demonstrate that AFLP polymorphisms can be informative for genetic characterization in Leishmania parasites, at both intra and inter-specific levels. |
| doi_str_mv | 10.1371/journal.pone.0073177 |
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In Panama, Leishmania parasites are endemic, causing thousands of new cases every year, mostly of the cutaneous form. In the last years, the burden of the disease has increased, coincident with increasing disturbances in its natural sylvatic environments. The study of genetic variation in parasites is important for a better understanding of the biology, population genetics, and ultimately the evolution and epidemiology of these organisms. Very few attempts have been made to characterize genetic polymorphisms of parasites isolated from Panamanian patients of cutaneous leishmaniasis. Here we present data on the genetic variability of local isolates of Leishmania, as well as specimens from several other species, by means of Amplified Fragment Length Polymorphisms (AFLP), a technique seldom used to study genetic makeup of parasites. We demonstrate that this technique allows detection of very high levels of genetic variability in local isolates of Leishmania panamensis in a highly reproducible manner. The analysis of AFLP fingerprints generated by unique selective primer combinations in L. panamensis suggests a predominant clonal mode of reproduction. Using fluorescently labeled primers, many taxon-specific fragments were identified which may show potential as species diagnostic fragments. The AFLP permitted a high resolution genetic analysis of the Leishmania genus, clearly separating certain groups among L. panamensis specimens and highly related species such as L. panamensis and L. guyanensis. The phylogenetic networks reconstructed from our AFLP data are congruent with established taxonomy for the genus Leishmania, even when using single selective primer combinations. Results of this study demonstrate that AFLP polymorphisms can be informative for genetic characterization in Leishmania parasites, at both intra and inter-specific levels.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0073177</identifier><identifier>PMID: 24039881</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amplified fragment length polymorphism ; Amplified Fragment Length Polymorphism Analysis ; Analysis ; Biological evolution ; Chromosomes ; Cutaneous leishmaniasis ; Deoxyribonucleic acid ; Diagnostic systems ; DNA ; Epidemiology ; Evolution, Molecular ; Fragmentation ; Fragments ; Gene expression ; Genetic analysis ; Genetic aspects ; Genetic diversity ; Genetic polymorphisms ; Genetic variability ; Genetics ; Genomes ; Health problems ; High resolution ; Humans ; Leishmania ; Leishmania - classification ; Leishmania - genetics ; Leishmaniasis ; Leishmaniasis, Cutaneous - epidemiology ; Multilocus Sequence Typing ; Neglected Diseases - epidemiology ; Panama - epidemiology ; Parasites ; Parasitic diseases ; Phylogeny ; Polymorphism ; Population genetics ; Primers ; Species ; Studies ; Taxonomy ; Tegumentary leishmaniasis ; Tropical diseases ; Variability ; Vector-borne diseases</subject><ispartof>PloS one, 2013-09, Vol.8 (9), p.e73177-e73177</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Restrepo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Restrepo et al 2013 Restrepo et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-c82dd4e0be65f681b3d02487031946db490f2b66c1230d3cf6c740fe57fc555c3</citedby><cites>FETCH-LOGICAL-c692t-c82dd4e0be65f681b3d02487031946db490f2b66c1230d3cf6c740fe57fc555c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3767818/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3767818/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24039881$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Restrepo, Carlos M</creatorcontrib><creatorcontrib>De La Guardia, Carolina</creatorcontrib><creatorcontrib>Sousa, Octavio E</creatorcontrib><creatorcontrib>Calzada, José E</creatorcontrib><creatorcontrib>Fernández, Patricia L</creatorcontrib><creatorcontrib>Lleonart, Ricardo</creatorcontrib><title>AFLP polymorphisms allow high resolution genetic analysis of American Tegumentary Leishmaniasis agents circulating in Panama and other members of the Leishmania genus</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>American Tegumentary Leishmaniasis is caused by parasites of the genus Leishmania, and causes significant health problems throughout the Americas. In Panama, Leishmania parasites are endemic, causing thousands of new cases every year, mostly of the cutaneous form. In the last years, the burden of the disease has increased, coincident with increasing disturbances in its natural sylvatic environments. The study of genetic variation in parasites is important for a better understanding of the biology, population genetics, and ultimately the evolution and epidemiology of these organisms. Very few attempts have been made to characterize genetic polymorphisms of parasites isolated from Panamanian patients of cutaneous leishmaniasis. Here we present data on the genetic variability of local isolates of Leishmania, as well as specimens from several other species, by means of Amplified Fragment Length Polymorphisms (AFLP), a technique seldom used to study genetic makeup of parasites. We demonstrate that this technique allows detection of very high levels of genetic variability in local isolates of Leishmania panamensis in a highly reproducible manner. The analysis of AFLP fingerprints generated by unique selective primer combinations in L. panamensis suggests a predominant clonal mode of reproduction. Using fluorescently labeled primers, many taxon-specific fragments were identified which may show potential as species diagnostic fragments. The AFLP permitted a high resolution genetic analysis of the Leishmania genus, clearly separating certain groups among L. panamensis specimens and highly related species such as L. panamensis and L. guyanensis. The phylogenetic networks reconstructed from our AFLP data are congruent with established taxonomy for the genus Leishmania, even when using single selective primer combinations. Results of this study demonstrate that AFLP polymorphisms can be informative for genetic characterization in Leishmania parasites, at both intra and inter-specific levels.</description><subject>Amplified fragment length polymorphism</subject><subject>Amplified Fragment Length Polymorphism Analysis</subject><subject>Analysis</subject><subject>Biological evolution</subject><subject>Chromosomes</subject><subject>Cutaneous leishmaniasis</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnostic systems</subject><subject>DNA</subject><subject>Epidemiology</subject><subject>Evolution, Molecular</subject><subject>Fragmentation</subject><subject>Fragments</subject><subject>Gene expression</subject><subject>Genetic analysis</subject><subject>Genetic aspects</subject><subject>Genetic diversity</subject><subject>Genetic polymorphisms</subject><subject>Genetic variability</subject><subject>Genetics</subject><subject>Genomes</subject><subject>Health problems</subject><subject>High resolution</subject><subject>Humans</subject><subject>Leishmania</subject><subject>Leishmania - classification</subject><subject>Leishmania - genetics</subject><subject>Leishmaniasis</subject><subject>Leishmaniasis, Cutaneous - epidemiology</subject><subject>Multilocus Sequence Typing</subject><subject>Neglected Diseases - epidemiology</subject><subject>Panama - epidemiology</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Phylogeny</subject><subject>Polymorphism</subject><subject>Population genetics</subject><subject>Primers</subject><subject>Species</subject><subject>Studies</subject><subject>Taxonomy</subject><subject>Tegumentary leishmaniasis</subject><subject>Tropical diseases</subject><subject>Variability</subject><subject>Vector-borne 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polymorphisms allow high resolution genetic analysis of American Tegumentary Leishmaniasis agents circulating in Panama and other members of the Leishmania genus</title><author>Restrepo, Carlos M ; De La Guardia, Carolina ; Sousa, Octavio E ; Calzada, José E ; Fernández, Patricia L ; Lleonart, Ricardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-c82dd4e0be65f681b3d02487031946db490f2b66c1230d3cf6c740fe57fc555c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amplified fragment length polymorphism</topic><topic>Amplified Fragment Length Polymorphism Analysis</topic><topic>Analysis</topic><topic>Biological evolution</topic><topic>Chromosomes</topic><topic>Cutaneous leishmaniasis</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnostic systems</topic><topic>DNA</topic><topic>Epidemiology</topic><topic>Evolution, Molecular</topic><topic>Fragmentation</topic><topic>Fragments</topic><topic>Gene expression</topic><topic>Genetic analysis</topic><topic>Genetic aspects</topic><topic>Genetic diversity</topic><topic>Genetic polymorphisms</topic><topic>Genetic variability</topic><topic>Genetics</topic><topic>Genomes</topic><topic>Health problems</topic><topic>High resolution</topic><topic>Humans</topic><topic>Leishmania</topic><topic>Leishmania - classification</topic><topic>Leishmania - genetics</topic><topic>Leishmaniasis</topic><topic>Leishmaniasis, Cutaneous - epidemiology</topic><topic>Multilocus Sequence Typing</topic><topic>Neglected Diseases - epidemiology</topic><topic>Panama - epidemiology</topic><topic>Parasites</topic><topic>Parasitic diseases</topic><topic>Phylogeny</topic><topic>Polymorphism</topic><topic>Population genetics</topic><topic>Primers</topic><topic>Species</topic><topic>Studies</topic><topic>Taxonomy</topic><topic>Tegumentary leishmaniasis</topic><topic>Tropical 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Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Restrepo, Carlos M</au><au>De La Guardia, Carolina</au><au>Sousa, Octavio E</au><au>Calzada, José E</au><au>Fernández, Patricia L</au><au>Lleonart, Ricardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AFLP polymorphisms allow high resolution genetic analysis of American Tegumentary Leishmaniasis agents circulating in Panama and other members of the Leishmania genus</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-09-09</date><risdate>2013</risdate><volume>8</volume><issue>9</issue><spage>e73177</spage><epage>e73177</epage><pages>e73177-e73177</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>American Tegumentary Leishmaniasis is caused by parasites of the genus Leishmania, and causes significant health problems throughout the Americas. In Panama, Leishmania parasites are endemic, causing thousands of new cases every year, mostly of the cutaneous form. In the last years, the burden of the disease has increased, coincident with increasing disturbances in its natural sylvatic environments. The study of genetic variation in parasites is important for a better understanding of the biology, population genetics, and ultimately the evolution and epidemiology of these organisms. Very few attempts have been made to characterize genetic polymorphisms of parasites isolated from Panamanian patients of cutaneous leishmaniasis. Here we present data on the genetic variability of local isolates of Leishmania, as well as specimens from several other species, by means of Amplified Fragment Length Polymorphisms (AFLP), a technique seldom used to study genetic makeup of parasites. We demonstrate that this technique allows detection of very high levels of genetic variability in local isolates of Leishmania panamensis in a highly reproducible manner. The analysis of AFLP fingerprints generated by unique selective primer combinations in L. panamensis suggests a predominant clonal mode of reproduction. Using fluorescently labeled primers, many taxon-specific fragments were identified which may show potential as species diagnostic fragments. The AFLP permitted a high resolution genetic analysis of the Leishmania genus, clearly separating certain groups among L. panamensis specimens and highly related species such as L. panamensis and L. guyanensis. The phylogenetic networks reconstructed from our AFLP data are congruent with established taxonomy for the genus Leishmania, even when using single selective primer combinations. Results of this study demonstrate that AFLP polymorphisms can be informative for genetic characterization in Leishmania parasites, at both intra and inter-specific levels.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24039881</pmid><doi>10.1371/journal.pone.0073177</doi><tpages>e73177</tpages><oa>free_for_read</oa></addata></record> |
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| source | PubMed (Medline); MEDLINE; Public Library of Science; DOAJ Directory of Open Access Journals; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Amplified fragment length polymorphism Amplified Fragment Length Polymorphism Analysis Analysis Biological evolution Chromosomes Cutaneous leishmaniasis Deoxyribonucleic acid Diagnostic systems DNA Epidemiology Evolution, Molecular Fragmentation Fragments Gene expression Genetic analysis Genetic aspects Genetic diversity Genetic polymorphisms Genetic variability Genetics Genomes Health problems High resolution Humans Leishmania Leishmania - classification Leishmania - genetics Leishmaniasis Leishmaniasis, Cutaneous - epidemiology Multilocus Sequence Typing Neglected Diseases - epidemiology Panama - epidemiology Parasites Parasitic diseases Phylogeny Polymorphism Population genetics Primers Species Studies Taxonomy Tegumentary leishmaniasis Tropical diseases Variability Vector-borne diseases |
| title | AFLP polymorphisms allow high resolution genetic analysis of American Tegumentary Leishmaniasis agents circulating in Panama and other members of the Leishmania genus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T19%3A08%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=AFLP%20polymorphisms%20allow%20high%20resolution%20genetic%20analysis%20of%20American%20Tegumentary%20Leishmaniasis%20agents%20circulating%20in%20Panama%20and%20other%20members%20of%20the%20Leishmania%20genus&rft.jtitle=PloS%20one&rft.au=Restrepo,%20Carlos%20M&rft.date=2013-09-09&rft.volume=8&rft.issue=9&rft.spage=e73177&rft.epage=e73177&rft.pages=e73177-e73177&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0073177&rft_dat=%3Cgale_plos_%3EA478353180%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1431103368&rft_id=info:pmid/24039881&rft_galeid=A478353180&rft_doaj_id=oai_doaj_org_article_1972306396124b1fac433d1c6486da9a&rfr_iscdi=true |