Aureolib - a proteome signature library: towards an understanding of staphylococcus aureus pathophysiology
Gel-based proteomics is a powerful approach to study the physiology of Staphylococcus aureus under various growth restricting conditions. We analyzed 679 protein spots from a reference 2-dimensional gel of cytosolic proteins of S. aureus COL by mass spectrometry resulting in 521 different proteins....
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| Veröffentlicht in: | PloS one 2013-08, Vol.8 (8), p.e70669 |
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| creator | Fuchs, Stephan Zühlke, Daniela Pané-Farré, Jan Kusch, Harald Wolf, Carmen Reiß, Swantje Binh, Le Thi Nguyen Albrecht, Dirk Riedel, Katharina Hecker, Michael Engelmann, Susanne |
| description | Gel-based proteomics is a powerful approach to study the physiology of Staphylococcus aureus under various growth restricting conditions. We analyzed 679 protein spots from a reference 2-dimensional gel of cytosolic proteins of S. aureus COL by mass spectrometry resulting in 521 different proteins. 4,692 time dependent protein synthesis profiles were generated by exposing S. aureus to nine infection-related stress and starvation stimuli (H2O2, diamide, paraquat, NO, fermentation, nitrate respiration, heat shock, puromycin, mupirocin). These expression profiles are stored in an online resource called Aureolib (http://www.aureolib.de). Moreover, information on target genes of 75 regulators and regulatory elements were included in the database. Cross-comparisons of this extensive data collection of protein synthesis profiles using the tools implemented in Aureolib lead to the identification of stress and starvation specific marker proteins. Altogether, 226 protein synthesis profiles showed induction ratios of 2.5-fold or higher under at least one of the tested conditions with 157 protein synthesis profiles specifically induced in response to a single stimulus. The respective proteins might serve as marker proteins for the corresponding stimulus. By contrast, proteins whose synthesis was increased or repressed in response to more than four stimuli are rather exceptional. The only protein that was induced by six stimuli is the universal stress protein SACOL1759. Most strikingly, cluster analyses of synthesis profiles of proteins differentially synthesized under at least one condition revealed only in rare cases a grouping that correlated with known regulon structures. The most prominent examples are the GapR, Rex, and CtsR regulon. In contrast, protein synthesis profiles of proteins belonging to the CodY and σ(B) regulon are widely distributed. In summary, Aureolib is by far the most comprehensive protein expression database for S. aureus and provides an essential tool to decipher more complex adaptation processes in S. aureus during host pathogen interaction. |
| doi_str_mv | 10.1371/journal.pone.0070669 |
| format | Article |
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We analyzed 679 protein spots from a reference 2-dimensional gel of cytosolic proteins of S. aureus COL by mass spectrometry resulting in 521 different proteins. 4,692 time dependent protein synthesis profiles were generated by exposing S. aureus to nine infection-related stress and starvation stimuli (H2O2, diamide, paraquat, NO, fermentation, nitrate respiration, heat shock, puromycin, mupirocin). These expression profiles are stored in an online resource called Aureolib (http://www.aureolib.de). Moreover, information on target genes of 75 regulators and regulatory elements were included in the database. Cross-comparisons of this extensive data collection of protein synthesis profiles using the tools implemented in Aureolib lead to the identification of stress and starvation specific marker proteins. Altogether, 226 protein synthesis profiles showed induction ratios of 2.5-fold or higher under at least one of the tested conditions with 157 protein synthesis profiles specifically induced in response to a single stimulus. The respective proteins might serve as marker proteins for the corresponding stimulus. By contrast, proteins whose synthesis was increased or repressed in response to more than four stimuli are rather exceptional. The only protein that was induced by six stimuli is the universal stress protein SACOL1759. Most strikingly, cluster analyses of synthesis profiles of proteins differentially synthesized under at least one condition revealed only in rare cases a grouping that correlated with known regulon structures. The most prominent examples are the GapR, Rex, and CtsR regulon. In contrast, protein synthesis profiles of proteins belonging to the CodY and σ(B) regulon are widely distributed. In summary, Aureolib is by far the most comprehensive protein expression database for S. aureus and provides an essential tool to decipher more complex adaptation processes in S. aureus during host pathogen interaction.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0070669</identifier><identifier>PMID: 23967085</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adaptation, Biological ; Antibacterial agents ; Bacillus subtilis ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biology ; Cluster Analysis ; Computational Biology - methods ; Computer Science ; Data collection ; Databases, Genetic ; Dehydrogenases ; Fermentation ; Gene expression ; Gene Expression Regulation, Bacterial ; Health aspects ; Heat shock ; Hydrogen peroxide ; Hydrogen Peroxide - metabolism ; Infection ; Mass spectrometry ; Mass spectroscopy ; Medicine ; Metabolism ; Mupirocin ; Neutrophils ; Nosocomial infections ; Online databases ; Paraquat ; Pathogens ; Physiological aspects ; Physiology ; Protein biosynthesis ; Protein folding ; Protein synthesis ; Proteins ; Proteome - metabolism ; Proteomics ; Proteomics - methods ; Puromycin ; Regulators ; Regulatory sequences ; Signal Transduction ; Spots ; Staphylococcus aureus ; Staphylococcus aureus - genetics ; Staphylococcus aureus - metabolism ; Staphylococcus aureus - pathogenicity ; Staphylococcus infections ; Starvation ; Stimuli ; Stress ; Stress, Physiological ; Stresses ; Studies ; Universal stress protein ; User-Computer Interface</subject><ispartof>PloS one, 2013-08, Vol.8 (8), p.e70669</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Fuchs et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Fuchs et al 2013 Fuchs et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-afd363823a6f445b74f70dec45a0882adc993978c8d60e6347672347d8e43273</citedby><cites>FETCH-LOGICAL-c692t-afd363823a6f445b74f70dec45a0882adc993978c8d60e6347672347d8e43273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742771/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742771/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79472,79473</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23967085$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Horsburgh, Malcolm James</contributor><creatorcontrib>Fuchs, Stephan</creatorcontrib><creatorcontrib>Zühlke, Daniela</creatorcontrib><creatorcontrib>Pané-Farré, Jan</creatorcontrib><creatorcontrib>Kusch, Harald</creatorcontrib><creatorcontrib>Wolf, Carmen</creatorcontrib><creatorcontrib>Reiß, Swantje</creatorcontrib><creatorcontrib>Binh, Le Thi Nguyen</creatorcontrib><creatorcontrib>Albrecht, Dirk</creatorcontrib><creatorcontrib>Riedel, Katharina</creatorcontrib><creatorcontrib>Hecker, Michael</creatorcontrib><creatorcontrib>Engelmann, Susanne</creatorcontrib><title>Aureolib - a proteome signature library: towards an understanding of staphylococcus aureus pathophysiology</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Gel-based proteomics is a powerful approach to study the physiology of Staphylococcus aureus under various growth restricting conditions. We analyzed 679 protein spots from a reference 2-dimensional gel of cytosolic proteins of S. aureus COL by mass spectrometry resulting in 521 different proteins. 4,692 time dependent protein synthesis profiles were generated by exposing S. aureus to nine infection-related stress and starvation stimuli (H2O2, diamide, paraquat, NO, fermentation, nitrate respiration, heat shock, puromycin, mupirocin). These expression profiles are stored in an online resource called Aureolib (http://www.aureolib.de). Moreover, information on target genes of 75 regulators and regulatory elements were included in the database. Cross-comparisons of this extensive data collection of protein synthesis profiles using the tools implemented in Aureolib lead to the identification of stress and starvation specific marker proteins. 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In summary, Aureolib is by far the most comprehensive protein expression database for S. aureus and provides an essential tool to decipher more complex adaptation processes in S. aureus during host pathogen interaction.</description><subject>Adaptation, Biological</subject><subject>Antibacterial agents</subject><subject>Bacillus subtilis</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biology</subject><subject>Cluster Analysis</subject><subject>Computational Biology - methods</subject><subject>Computer Science</subject><subject>Data collection</subject><subject>Databases, Genetic</subject><subject>Dehydrogenases</subject><subject>Fermentation</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Health aspects</subject><subject>Heat shock</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Infection</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medicine</subject><subject>Metabolism</subject><subject>Mupirocin</subject><subject>Neutrophils</subject><subject>Nosocomial infections</subject><subject>Online databases</subject><subject>Paraquat</subject><subject>Pathogens</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Protein biosynthesis</subject><subject>Protein folding</subject><subject>Protein synthesis</subject><subject>Proteins</subject><subject>Proteome - metabolism</subject><subject>Proteomics</subject><subject>Proteomics - methods</subject><subject>Puromycin</subject><subject>Regulators</subject><subject>Regulatory sequences</subject><subject>Signal Transduction</subject><subject>Spots</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - metabolism</subject><subject>Staphylococcus aureus - pathogenicity</subject><subject>Staphylococcus infections</subject><subject>Starvation</subject><subject>Stimuli</subject><subject>Stress</subject><subject>Stress, Physiological</subject><subject>Stresses</subject><subject>Studies</subject><subject>Universal stress protein</subject><subject>User-Computer Interface</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7rr6D0QLguDFjPlq0nohLIsfAwsLungbziRpJ0MnqUmqzr8343SXKShIIAnnPO_JyeEtiucYLTEV-O3Wj8FBvxy8M0uEBOK8eVCc44aSBSeIPjy5nxVPYtwiVNGa88fFGaENF6iuzovt5RiM7-26XJRQDsEn43emjLZzkHKqzKkAYf-uTP4nBB1LcOXotAkxgdPWdaVvy3wfNvveK6_UmJEszMcAaeNzPFrf-27_tHjUQh_Ns-m8KG4_fri9-ry4vvm0urq8XijekLSAVlNOa0KBt4xVa8FagbRRrAJU1wS0ahraiFrVmiPDKRNckLzr2jBKBL0oXh7LDr2PcppSlJhRxBrGEM7E6khoD1s5BLvLH5QerPwT8KGTEJJVvZGcVmSN9Lqt2oZhWgGrNMccFGZgMDu89n56bVzvjFbGpQD9rOg84-xGdv6HpIIRIQ7NvJoKBP99NDH9o-WJ6iB3ZV3rczG1s1HJSyZq0tSEs0wt_0Llpc3OquyT1ub4TPBmJshMMr9SB2OMcvX1y_-zN9_m7OsTdmOgT5vo-zFZ7-IcZEdQBR9jMO395DCSB5vfTUMebC4nm2fZi9Op34vufE1_A5_a-QU</recordid><startdate>20130813</startdate><enddate>20130813</enddate><creator>Fuchs, Stephan</creator><creator>Zühlke, Daniela</creator><creator>Pané-Farré, Jan</creator><creator>Kusch, Harald</creator><creator>Wolf, Carmen</creator><creator>Reiß, Swantje</creator><creator>Binh, Le Thi Nguyen</creator><creator>Albrecht, Dirk</creator><creator>Riedel, Katharina</creator><creator>Hecker, Michael</creator><creator>Engelmann, Susanne</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130813</creationdate><title>Aureolib - a proteome signature library: towards an understanding of staphylococcus aureus pathophysiology</title><author>Fuchs, Stephan ; Zühlke, Daniela ; Pané-Farré, Jan ; Kusch, Harald ; Wolf, Carmen ; Reiß, Swantje ; Binh, Le Thi Nguyen ; Albrecht, Dirk ; Riedel, Katharina ; Hecker, Michael ; Engelmann, Susanne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-afd363823a6f445b74f70dec45a0882adc993978c8d60e6347672347d8e43273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adaptation, Biological</topic><topic>Antibacterial agents</topic><topic>Bacillus subtilis</topic><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biology</topic><topic>Cluster Analysis</topic><topic>Computational Biology - methods</topic><topic>Computer Science</topic><topic>Data collection</topic><topic>Databases, Genetic</topic><topic>Dehydrogenases</topic><topic>Fermentation</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Health aspects</topic><topic>Heat shock</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Infection</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medicine</topic><topic>Metabolism</topic><topic>Mupirocin</topic><topic>Neutrophils</topic><topic>Nosocomial infections</topic><topic>Online databases</topic><topic>Paraquat</topic><topic>Pathogens</topic><topic>Physiological aspects</topic><topic>Physiology</topic><topic>Protein biosynthesis</topic><topic>Protein folding</topic><topic>Protein synthesis</topic><topic>Proteins</topic><topic>Proteome - metabolism</topic><topic>Proteomics</topic><topic>Proteomics - methods</topic><topic>Puromycin</topic><topic>Regulators</topic><topic>Regulatory sequences</topic><topic>Signal Transduction</topic><topic>Spots</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - metabolism</topic><topic>Staphylococcus aureus - pathogenicity</topic><topic>Staphylococcus infections</topic><topic>Starvation</topic><topic>Stimuli</topic><topic>Stress</topic><topic>Stress, Physiological</topic><topic>Stresses</topic><topic>Studies</topic><topic>Universal stress protein</topic><topic>User-Computer Interface</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fuchs, Stephan</creatorcontrib><creatorcontrib>Zühlke, Daniela</creatorcontrib><creatorcontrib>Pané-Farré, Jan</creatorcontrib><creatorcontrib>Kusch, Harald</creatorcontrib><creatorcontrib>Wolf, Carmen</creatorcontrib><creatorcontrib>Reiß, Swantje</creatorcontrib><creatorcontrib>Binh, Le Thi Nguyen</creatorcontrib><creatorcontrib>Albrecht, Dirk</creatorcontrib><creatorcontrib>Riedel, Katharina</creatorcontrib><creatorcontrib>Hecker, Michael</creatorcontrib><creatorcontrib>Engelmann, Susanne</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Agriculture & Environmental Science Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fuchs, Stephan</au><au>Zühlke, Daniela</au><au>Pané-Farré, Jan</au><au>Kusch, Harald</au><au>Wolf, Carmen</au><au>Reiß, Swantje</au><au>Binh, Le Thi Nguyen</au><au>Albrecht, Dirk</au><au>Riedel, Katharina</au><au>Hecker, Michael</au><au>Engelmann, Susanne</au><au>Horsburgh, Malcolm James</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aureolib - a proteome signature library: towards an understanding of staphylococcus aureus pathophysiology</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-08-13</date><risdate>2013</risdate><volume>8</volume><issue>8</issue><spage>e70669</spage><pages>e70669-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Gel-based proteomics is a powerful approach to study the physiology of Staphylococcus aureus under various growth restricting conditions. We analyzed 679 protein spots from a reference 2-dimensional gel of cytosolic proteins of S. aureus COL by mass spectrometry resulting in 521 different proteins. 4,692 time dependent protein synthesis profiles were generated by exposing S. aureus to nine infection-related stress and starvation stimuli (H2O2, diamide, paraquat, NO, fermentation, nitrate respiration, heat shock, puromycin, mupirocin). These expression profiles are stored in an online resource called Aureolib (http://www.aureolib.de). Moreover, information on target genes of 75 regulators and regulatory elements were included in the database. Cross-comparisons of this extensive data collection of protein synthesis profiles using the tools implemented in Aureolib lead to the identification of stress and starvation specific marker proteins. Altogether, 226 protein synthesis profiles showed induction ratios of 2.5-fold or higher under at least one of the tested conditions with 157 protein synthesis profiles specifically induced in response to a single stimulus. The respective proteins might serve as marker proteins for the corresponding stimulus. By contrast, proteins whose synthesis was increased or repressed in response to more than four stimuli are rather exceptional. The only protein that was induced by six stimuli is the universal stress protein SACOL1759. Most strikingly, cluster analyses of synthesis profiles of proteins differentially synthesized under at least one condition revealed only in rare cases a grouping that correlated with known regulon structures. The most prominent examples are the GapR, Rex, and CtsR regulon. In contrast, protein synthesis profiles of proteins belonging to the CodY and σ(B) regulon are widely distributed. In summary, Aureolib is by far the most comprehensive protein expression database for S. aureus and provides an essential tool to decipher more complex adaptation processes in S. aureus during host pathogen interaction.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23967085</pmid><doi>10.1371/journal.pone.0070669</doi><tpages>e70669</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-08, Vol.8 (8), p.e70669 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1430494401 |
| source | Open Access: DOAJ - Directory of Open Access Journals; Open Access: PubMed Central; PLoS; MEDLINE; Full-Text Journals in Chemistry (Open access); EZB Electronic Journals Library |
| subjects | Adaptation, Biological Antibacterial agents Bacillus subtilis Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Biology Cluster Analysis Computational Biology - methods Computer Science Data collection Databases, Genetic Dehydrogenases Fermentation Gene expression Gene Expression Regulation, Bacterial Health aspects Heat shock Hydrogen peroxide Hydrogen Peroxide - metabolism Infection Mass spectrometry Mass spectroscopy Medicine Metabolism Mupirocin Neutrophils Nosocomial infections Online databases Paraquat Pathogens Physiological aspects Physiology Protein biosynthesis Protein folding Protein synthesis Proteins Proteome - metabolism Proteomics Proteomics - methods Puromycin Regulators Regulatory sequences Signal Transduction Spots Staphylococcus aureus Staphylococcus aureus - genetics Staphylococcus aureus - metabolism Staphylococcus aureus - pathogenicity Staphylococcus infections Starvation Stimuli Stress Stress, Physiological Stresses Studies Universal stress protein User-Computer Interface |
| title | Aureolib - a proteome signature library: towards an understanding of staphylococcus aureus pathophysiology |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T19%3A33%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Aureolib%20-%20a%20proteome%20signature%20library:%20towards%20an%20understanding%20of%20staphylococcus%20aureus%20pathophysiology&rft.jtitle=PloS%20one&rft.au=Fuchs,%20Stephan&rft.date=2013-08-13&rft.volume=8&rft.issue=8&rft.spage=e70669&rft.pages=e70669-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0070669&rft_dat=%3Cgale_plos_%3EA478298264%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1430494401&rft_id=info:pmid/23967085&rft_galeid=A478298264&rft_doaj_id=oai_doaj_org_article_6352b0dbf5f94135a45d616ac14ae147&rfr_iscdi=true |