A simple approach for COnsumption and RElease (CORE) analysis of metabolic activity in single mammalian embryos
Non-invasive assay of the consumption and release of metabolites by individual human embryos could allow selection at the cleavage stage of development and facilitate Single Embryo Transfer in clinical IVF but will require simple, high throughput, sensitive methods applicable to small volume samples...
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| Veröffentlicht in: | PloS one 2013-08, Vol.8 (8), p.e67834-e67834 |
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| creator | Guerif, Fabrice McKeegan, Paul Leese, Henry J Sturmey, Roger G |
| description | Non-invasive assay of the consumption and release of metabolites by individual human embryos could allow selection at the cleavage stage of development and facilitate Single Embryo Transfer in clinical IVF but will require simple, high throughput, sensitive methods applicable to small volume samples. A rapid, simple, non-invasive method has therefore been devised using a standard fluorescence plate reader, and used to measure the consumption of pyruvate and glucose, and release of lactate by single bovine embryos at all stages of preimplantation development in culture; amino acid profiles have been determined using HPLC. Early embryos with an 'intermediate' level (6.14±0.27 pmol/embryo/h) of pyruvate uptake were associated with the highest rate (68.3%) of blastocyst development indicating that a mid "optimum" range of pyruvate consumption correlates with high viability in this bovine model. |
| doi_str_mv | 10.1371/journal.pone.0067834 |
| format | Article |
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A rapid, simple, non-invasive method has therefore been devised using a standard fluorescence plate reader, and used to measure the consumption of pyruvate and glucose, and release of lactate by single bovine embryos at all stages of preimplantation development in culture; amino acid profiles have been determined using HPLC. Early embryos with an 'intermediate' level (6.14±0.27 pmol/embryo/h) of pyruvate uptake were associated with the highest rate (68.3%) of blastocyst development indicating that a mid "optimum" range of pyruvate consumption correlates with high viability in this bovine model.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0067834</identifier><identifier>PMID: 23967049</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amino acids ; Amino Acids - metabolism ; Analysis ; Animals ; Biology ; Biomarkers ; Carbohydrate Metabolism ; Cattle ; Chemistry ; Consumption ; Consumption data ; Developmental stages ; Embryo transfer ; Embryo, Mammalian - metabolism ; Embryonic development ; Embryos ; Energy ; Fluorescence ; Gene expression ; Glucose ; Glucose - metabolism ; Growth factors ; High performance liquid chromatography ; Humans ; Laboratories ; Lactic acid ; Lactic Acid - metabolism ; Life Sciences ; Liquid chromatography ; Medical schools ; Medicine ; Metabolism ; Metabolites ; Metabolome ; Other ; Polyvinyl alcohol ; Pyruvates - metabolism ; Pyruvic acid ; Viability</subject><ispartof>PloS one, 2013-08, Vol.8 (8), p.e67834-e67834</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Guerif et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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A rapid, simple, non-invasive method has therefore been devised using a standard fluorescence plate reader, and used to measure the consumption of pyruvate and glucose, and release of lactate by single bovine embryos at all stages of preimplantation development in culture; amino acid profiles have been determined using HPLC. Early embryos with an 'intermediate' level (6.14±0.27 pmol/embryo/h) of pyruvate uptake were associated with the highest rate (68.3%) of blastocyst development indicating that a mid "optimum" range of pyruvate consumption correlates with high viability in this bovine model.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23967049</pmid><doi>10.1371/journal.pone.0067834</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-4340-0170</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Amino acids Amino Acids - metabolism Analysis Animals Biology Biomarkers Carbohydrate Metabolism Cattle Chemistry Consumption Consumption data Developmental stages Embryo transfer Embryo, Mammalian - metabolism Embryonic development Embryos Energy Fluorescence Gene expression Glucose Glucose - metabolism Growth factors High performance liquid chromatography Humans Laboratories Lactic acid Lactic Acid - metabolism Life Sciences Liquid chromatography Medical schools Medicine Metabolism Metabolites Metabolome Other Polyvinyl alcohol Pyruvates - metabolism Pyruvic acid Viability |
| title | A simple approach for COnsumption and RElease (CORE) analysis of metabolic activity in single mammalian embryos |
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