ABC transporters in Dictyostelium discoideum development
ATP-binding cassette (ABC) transporters can translocate a broad spectrum of molecules across the cell membrane including physiological cargo and toxins. ABC transporters are known for the role they play in resistance towards anticancer agents in chemotherapy of cancer patients. There are 68 ABC tran...
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| Veröffentlicht in: | PloS one 2013-08, Vol.8 (8), p.e70040-e70040 |
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| creator | Miranda, Edward Roshan Zhuchenko, Olga Toplak, Marko Santhanam, Balaji Zupan, Blaz Kuspa, Adam Shaulsky, Gad |
| description | ATP-binding cassette (ABC) transporters can translocate a broad spectrum of molecules across the cell membrane including physiological cargo and toxins. ABC transporters are known for the role they play in resistance towards anticancer agents in chemotherapy of cancer patients. There are 68 ABC transporters annotated in the genome of the social amoeba Dictyostelium discoideum. We have characterized more than half of these ABC transporters through a systematic study of mutations in their genes. We have analyzed morphological and transcriptional phenotypes for these mutants during growth and development and found that most of the mutants exhibited rather subtle phenotypes. A few of the genes may share physiological functions, as reflected in their transcriptional phenotypes. Since most of the abc-transporter mutants showed subtle morphological phenotypes, we utilized these transcriptional phenotypes to identify genes that are important for development by looking for transcripts whose abundance was unperturbed in most of the mutants. We found a set of 668 genes that includes many validated D. discoideum developmental genes. We have also found that abcG6 and abcG18 may have potential roles in intercellular signaling during terminal differentiation of spores and stalks. |
| doi_str_mv | 10.1371/journal.pone.0070040 |
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ABC transporters are known for the role they play in resistance towards anticancer agents in chemotherapy of cancer patients. There are 68 ABC transporters annotated in the genome of the social amoeba Dictyostelium discoideum. We have characterized more than half of these ABC transporters through a systematic study of mutations in their genes. We have analyzed morphological and transcriptional phenotypes for these mutants during growth and development and found that most of the mutants exhibited rather subtle phenotypes. A few of the genes may share physiological functions, as reflected in their transcriptional phenotypes. Since most of the abc-transporter mutants showed subtle morphological phenotypes, we utilized these transcriptional phenotypes to identify genes that are important for development by looking for transcripts whose abundance was unperturbed in most of the mutants. We found a set of 668 genes that includes many validated D. discoideum developmental genes. We have also found that abcG6 and abcG18 may have potential roles in intercellular signaling during terminal differentiation of spores and stalks.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0070040</identifier><identifier>PMID: 23967067</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>ABC transporters ; Amoeba ; Analysis ; Anticancer properties ; Antitumor agents ; ATP-Binding Cassette Transporters - genetics ; ATP-Binding Cassette Transporters - metabolism ; Biochemistry ; Biology ; Biophysics ; Cancer ; Cell Differentiation - genetics ; Chemotherapy ; Cloning ; Developmental biology ; Dictyostelium - cytology ; Dictyostelium - genetics ; Dictyostelium - growth & development ; Dictyostelium - metabolism ; Gene expression ; Genes ; Genetic aspects ; Genetics ; Genomes ; Genomics ; Information science ; Kinases ; Medicine ; Membranes ; Molecular biology ; Morphology ; Mutants ; Mutation ; Phenotype ; Physiology ; Proteins ; Protozoan Proteins - genetics ; Protozoan Proteins - metabolism ; Signaling ; Spores ; Spores, Protozoan - cytology ; Spores, Protozoan - genetics ; Spores, Protozoan - growth & development ; Spores, Protozoan - metabolism ; Toxins ; Transcription ; Transcription (Genetics) ; Transcription factors ; Transcription, Genetic</subject><ispartof>PloS one, 2013-08, Vol.8 (8), p.e70040-e70040</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Miranda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Miranda et al 2013 Miranda et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-298a6702b4059dc4993c3222065ff84b342ab22519a1958ec7fdeba6bff760313</citedby><cites>FETCH-LOGICAL-c692t-298a6702b4059dc4993c3222065ff84b342ab22519a1958ec7fdeba6bff760313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3743828/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3743828/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23870,27928,27929,53795,53797</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23967067$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>van Veen, Hendrik W.</contributor><creatorcontrib>Miranda, Edward Roshan</creatorcontrib><creatorcontrib>Zhuchenko, Olga</creatorcontrib><creatorcontrib>Toplak, Marko</creatorcontrib><creatorcontrib>Santhanam, Balaji</creatorcontrib><creatorcontrib>Zupan, Blaz</creatorcontrib><creatorcontrib>Kuspa, Adam</creatorcontrib><creatorcontrib>Shaulsky, Gad</creatorcontrib><title>ABC transporters in Dictyostelium discoideum development</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>ATP-binding cassette (ABC) transporters can translocate a broad spectrum of molecules across the cell membrane including physiological cargo and toxins. 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We have also found that abcG6 and abcG18 may have potential roles in intercellular signaling during terminal differentiation of spores and stalks.</description><subject>ABC transporters</subject><subject>Amoeba</subject><subject>Analysis</subject><subject>Anticancer properties</subject><subject>Antitumor agents</subject><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>ATP-Binding Cassette Transporters - metabolism</subject><subject>Biochemistry</subject><subject>Biology</subject><subject>Biophysics</subject><subject>Cancer</subject><subject>Cell Differentiation - genetics</subject><subject>Chemotherapy</subject><subject>Cloning</subject><subject>Developmental biology</subject><subject>Dictyostelium - cytology</subject><subject>Dictyostelium - genetics</subject><subject>Dictyostelium - growth & development</subject><subject>Dictyostelium - metabolism</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetics</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Information science</subject><subject>Kinases</subject><subject>Medicine</subject><subject>Membranes</subject><subject>Molecular biology</subject><subject>Morphology</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Phenotype</subject><subject>Physiology</subject><subject>Proteins</subject><subject>Protozoan Proteins - 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ABC transporters are known for the role they play in resistance towards anticancer agents in chemotherapy of cancer patients. There are 68 ABC transporters annotated in the genome of the social amoeba Dictyostelium discoideum. We have characterized more than half of these ABC transporters through a systematic study of mutations in their genes. We have analyzed morphological and transcriptional phenotypes for these mutants during growth and development and found that most of the mutants exhibited rather subtle phenotypes. A few of the genes may share physiological functions, as reflected in their transcriptional phenotypes. Since most of the abc-transporter mutants showed subtle morphological phenotypes, we utilized these transcriptional phenotypes to identify genes that are important for development by looking for transcripts whose abundance was unperturbed in most of the mutants. We found a set of 668 genes that includes many validated D. discoideum developmental genes. We have also found that abcG6 and abcG18 may have potential roles in intercellular signaling during terminal differentiation of spores and stalks.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23967067</pmid><doi>10.1371/journal.pone.0070040</doi><oa>free_for_read</oa></addata></record> |
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| subjects | ABC transporters Amoeba Analysis Anticancer properties Antitumor agents ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism Biochemistry Biology Biophysics Cancer Cell Differentiation - genetics Chemotherapy Cloning Developmental biology Dictyostelium - cytology Dictyostelium - genetics Dictyostelium - growth & development Dictyostelium - metabolism Gene expression Genes Genetic aspects Genetics Genomes Genomics Information science Kinases Medicine Membranes Molecular biology Morphology Mutants Mutation Phenotype Physiology Proteins Protozoan Proteins - genetics Protozoan Proteins - metabolism Signaling Spores Spores, Protozoan - cytology Spores, Protozoan - genetics Spores, Protozoan - growth & development Spores, Protozoan - metabolism Toxins Transcription Transcription (Genetics) Transcription factors Transcription, Genetic |
| title | ABC transporters in Dictyostelium discoideum development |
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