Annexin A2 promotes the migration and invasion of human hepatocellular carcinoma cells in vitro by regulating the shedding of CD147-harboring microvesicles from tumor cells
It has been reported that Annexin A2 (ANXA2) is up-regulated in hepatocellular carcinoma (HCC), but the roles of ANXA2 in the migration and invasion of HCC cells have not been determined. In this study, we found that ANXA2-specific siRNA (si-ANXA2) significantly inhibited the migration and invasion...
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| creator | Zhang, Wei Zhao, Pu Xu, Xiu-Li Cai, Lei Song, Zhen-Shun Cao, Da-Yong Tao, Kai-Shan Zhou, Wen-Ping Chen, Zhi-Nan Dou, Ke-Feng |
| description | It has been reported that Annexin A2 (ANXA2) is up-regulated in hepatocellular carcinoma (HCC), but the roles of ANXA2 in the migration and invasion of HCC cells have not been determined. In this study, we found that ANXA2-specific siRNA (si-ANXA2) significantly inhibited the migration and invasion of HCC cells co-cultured with fibroblasts in vitro. In addition, the production of MMP-2 by fibroblasts cultured in supernatant collected from si-ANXA2-transfected HCC cells was notably down-regulated. ANXA2 was also found to be co-localized and co-immunoprecipitated with CD147. Further investigation revealed that the expression of ANXA2 in HCC cells affected the shedding of CD147-harboring membrane microvesicles, acting as a vehicle for CD147 in tumor-stromal interactions and thereby regulating the production of MMP-2 by fibroblasts. Together, these results suggest that ANXA2 enhances the migration and invasion potential of HCC cells in vitro by regulating the trafficking of CD147-harboring membrane microvesicles. |
| doi_str_mv | 10.1371/journal.pone.0067268 |
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In this study, we found that ANXA2-specific siRNA (si-ANXA2) significantly inhibited the migration and invasion of HCC cells co-cultured with fibroblasts in vitro. In addition, the production of MMP-2 by fibroblasts cultured in supernatant collected from si-ANXA2-transfected HCC cells was notably down-regulated. ANXA2 was also found to be co-localized and co-immunoprecipitated with CD147. Further investigation revealed that the expression of ANXA2 in HCC cells affected the shedding of CD147-harboring membrane microvesicles, acting as a vehicle for CD147 in tumor-stromal interactions and thereby regulating the production of MMP-2 by fibroblasts. Together, these results suggest that ANXA2 enhances the migration and invasion potential of HCC cells in vitro by regulating the trafficking of CD147-harboring membrane microvesicles.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0067268</identifier><identifier>PMID: 23950866</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Angiogenesis ; Annexin A2 - genetics ; Annexin A2 - metabolism ; Annexins ; Basigin - metabolism ; Binding sites ; Biology ; Carcinoma, Hepatocellular - genetics ; Carcinoma, Hepatocellular - metabolism ; Carcinoma, Hepatocellular - pathology ; CD147 antigen ; Cell Line, Tumor ; Cell Membrane - metabolism ; Cell Movement - genetics ; Chemistry ; Coculture Techniques ; Engineering research ; Extracellular matrix ; Fibroblasts ; Gelatinase A ; Gene Expression Regulation, Neoplastic ; Hepatocellular carcinoma ; Humans ; Immunoglobulins ; Kinases ; Laboratories ; Liver cancer ; Liver Neoplasms - genetics ; Liver Neoplasms - metabolism ; Liver Neoplasms - pathology ; Medicine ; Membrane trafficking ; Metastasis ; Microvessels - metabolism ; Microvessels - pathology ; Neoplasm Invasiveness ; Neovascularization, Pathologic - genetics ; Neovascularization, Pathologic - metabolism ; Prostate cancer ; Protein Binding ; Protein Transport ; Proteins ; RNA Interference ; Shedding ; Signal transduction ; siRNA ; Surgery ; Tumor cells ; Tumors</subject><ispartof>PloS one, 2013-08, Vol.8 (8), p.e67268-e67268</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Together, these results suggest that ANXA2 enhances the migration and invasion potential of HCC cells in vitro by regulating the trafficking of CD147-harboring membrane microvesicles.</description><subject>Angiogenesis</subject><subject>Annexin A2 - genetics</subject><subject>Annexin A2 - metabolism</subject><subject>Annexins</subject><subject>Basigin - metabolism</subject><subject>Binding sites</subject><subject>Biology</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>CD147 antigen</subject><subject>Cell Line, Tumor</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Movement - genetics</subject><subject>Chemistry</subject><subject>Coculture Techniques</subject><subject>Engineering research</subject><subject>Extracellular matrix</subject><subject>Fibroblasts</subject><subject>Gelatinase A</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Hepatocellular carcinoma</subject><subject>Humans</subject><subject>Immunoglobulins</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Liver cancer</subject><subject>Liver Neoplasms - 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genetics</topic><topic>Annexin A2 - metabolism</topic><topic>Annexins</topic><topic>Basigin - metabolism</topic><topic>Binding sites</topic><topic>Biology</topic><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>CD147 antigen</topic><topic>Cell Line, Tumor</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Movement - genetics</topic><topic>Chemistry</topic><topic>Coculture Techniques</topic><topic>Engineering research</topic><topic>Extracellular matrix</topic><topic>Fibroblasts</topic><topic>Gelatinase A</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Hepatocellular carcinoma</topic><topic>Humans</topic><topic>Immunoglobulins</topic><topic>Kinases</topic><topic>Laboratories</topic><topic>Liver cancer</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - metabolism</topic><topic>Liver Neoplasms - pathology</topic><topic>Medicine</topic><topic>Membrane trafficking</topic><topic>Metastasis</topic><topic>Microvessels - 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In this study, we found that ANXA2-specific siRNA (si-ANXA2) significantly inhibited the migration and invasion of HCC cells co-cultured with fibroblasts in vitro. In addition, the production of MMP-2 by fibroblasts cultured in supernatant collected from si-ANXA2-transfected HCC cells was notably down-regulated. ANXA2 was also found to be co-localized and co-immunoprecipitated with CD147. Further investigation revealed that the expression of ANXA2 in HCC cells affected the shedding of CD147-harboring membrane microvesicles, acting as a vehicle for CD147 in tumor-stromal interactions and thereby regulating the production of MMP-2 by fibroblasts. Together, these results suggest that ANXA2 enhances the migration and invasion potential of HCC cells in vitro by regulating the trafficking of CD147-harboring membrane microvesicles.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23950866</pmid><doi>10.1371/journal.pone.0067268</doi><tpages>e67268</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-08, Vol.8 (8), p.e67268-e67268 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1430420663 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Angiogenesis Annexin A2 - genetics Annexin A2 - metabolism Annexins Basigin - metabolism Binding sites Biology Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - pathology CD147 antigen Cell Line, Tumor Cell Membrane - metabolism Cell Movement - genetics Chemistry Coculture Techniques Engineering research Extracellular matrix Fibroblasts Gelatinase A Gene Expression Regulation, Neoplastic Hepatocellular carcinoma Humans Immunoglobulins Kinases Laboratories Liver cancer Liver Neoplasms - genetics Liver Neoplasms - metabolism Liver Neoplasms - pathology Medicine Membrane trafficking Metastasis Microvessels - metabolism Microvessels - pathology Neoplasm Invasiveness Neovascularization, Pathologic - genetics Neovascularization, Pathologic - metabolism Prostate cancer Protein Binding Protein Transport Proteins RNA Interference Shedding Signal transduction siRNA Surgery Tumor cells Tumors |
| title | Annexin A2 promotes the migration and invasion of human hepatocellular carcinoma cells in vitro by regulating the shedding of CD147-harboring microvesicles from tumor cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T20%3A43%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Annexin%20A2%20promotes%20the%20migration%20and%20invasion%20of%20human%20hepatocellular%20carcinoma%20cells%20in%20vitro%20by%20regulating%20the%20shedding%20of%20CD147-harboring%20microvesicles%20from%20tumor%20cells&rft.jtitle=PloS%20one&rft.au=Zhang,%20Wei&rft.date=2013-08-12&rft.volume=8&rft.issue=8&rft.spage=e67268&rft.epage=e67268&rft.pages=e67268-e67268&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0067268&rft_dat=%3Cgale_plos_%3EA478310911%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1430420663&rft_id=info:pmid/23950866&rft_galeid=A478310911&rft_doaj_id=oai_doaj_org_article_4b2c63386e92468dbcc5df34507a1ef8&rfr_iscdi=true |