Nanosecond pulsed electric field suppresses development of eyes and germ cells through blocking synthesis of retinoic acid in Medaka (Oryzias latipes)
Application of nanosecond pulsed electric fields (nsPEFs) has attracted rising attention in various scientific fields including medical, pharmacological, and biological sciences, although its effects and molecular mechanisms leading to the effects remain poorly understood. Here, we show that a singl...
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| creator | Shiraishi, Eri Hosseini, Hamid Kang, Dong K Kitano, Takeshi Akiyama, Hidenori |
| description | Application of nanosecond pulsed electric fields (nsPEFs) has attracted rising attention in various scientific fields including medical, pharmacological, and biological sciences, although its effects and molecular mechanisms leading to the effects remain poorly understood. Here, we show that a single, high-intensity (10-30 kV/cm), 60-ns PEF exposure affects gene expression and impairs development of eyes and germ cells in medaka (Oryzias latipes). Exposure of early blastula stage embryos to nsPEF down-regulated the expression of several transcription factors which are essential for eye development, causing abnormal eye formation. Moreover, the majority of the exposed genetic female embryos showed a fewer number of germ cells similar to that of the control (unexposed) genetic male at 9 days post-fertilization (dpf). However, all-trans retinoic acid (atRA) treatment following the exposure rescued proliferation of germ cells and resumption of normal eye development, suggesting that the phenotypes induced by nsPEF are caused by a decrease of retinoic acid levels. These results confirm that nsPEFs induce novel effects during embryogenesis in medaka. |
| doi_str_mv | 10.1371/journal.pone.0070670 |
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Here, we show that a single, high-intensity (10-30 kV/cm), 60-ns PEF exposure affects gene expression and impairs development of eyes and germ cells in medaka (Oryzias latipes). Exposure of early blastula stage embryos to nsPEF down-regulated the expression of several transcription factors which are essential for eye development, causing abnormal eye formation. Moreover, the majority of the exposed genetic female embryos showed a fewer number of germ cells similar to that of the control (unexposed) genetic male at 9 days post-fertilization (dpf). However, all-trans retinoic acid (atRA) treatment following the exposure rescued proliferation of germ cells and resumption of normal eye development, suggesting that the phenotypes induced by nsPEF are caused by a decrease of retinoic acid levels. These results confirm that nsPEFs induce novel effects during embryogenesis in medaka.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0070670</identifier><identifier>PMID: 23936463</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Animals ; Biological effects ; Biology ; Blastula ; Cell Proliferation ; Dehydrogenases ; Deoxyribonucleic acid ; DNA ; DNA binding proteins ; Electric fields ; Electroporation - methods ; Embryogenesis ; Embryonic Development ; Embryonic growth stage ; Embryos ; Enzymes ; Exposure ; Eye ; Eye - embryology ; Eye - metabolism ; Female ; Fertilization ; Gene expression ; Gene Expression Regulation, Developmental ; Genes ; Genetic engineering ; Genomes ; Germ cells ; Male ; Medicine ; Metabolism ; Molecular modelling ; Mutation ; Nuclear Proteins - genetics ; Oryzias - embryology ; Oryzias latipes ; Ovum - cytology ; Ovum - metabolism ; Pharmacology ; Physics ; Retinoic acid ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Rodents ; Science ; Spermatozoa - cytology ; Spermatozoa - metabolism ; Studies ; Time Factors ; Transcription factors ; Tretinoin ; Tretinoin - metabolism ; Zebrafish</subject><ispartof>PloS one, 2013-08, Vol.8 (8), p.e70670-e70670</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Shiraishi et al. 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These results confirm that nsPEFs induce novel effects during embryogenesis in medaka.</description><subject>Acids</subject><subject>Animals</subject><subject>Biological effects</subject><subject>Biology</subject><subject>Blastula</subject><subject>Cell Proliferation</subject><subject>Dehydrogenases</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA binding proteins</subject><subject>Electric fields</subject><subject>Electroporation - methods</subject><subject>Embryogenesis</subject><subject>Embryonic Development</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Enzymes</subject><subject>Exposure</subject><subject>Eye</subject><subject>Eye - embryology</subject><subject>Eye - metabolism</subject><subject>Female</subject><subject>Fertilization</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genomes</subject><subject>Germ cells</subject><subject>Male</subject><subject>Medicine</subject><subject>Metabolism</subject><subject>Molecular modelling</subject><subject>Mutation</subject><subject>Nuclear Proteins - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shiraishi, Eri</au><au>Hosseini, Hamid</au><au>Kang, Dong K</au><au>Kitano, Takeshi</au><au>Akiyama, Hidenori</au><au>Scarfi, Maria Rosaria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanosecond pulsed electric field suppresses development of eyes and germ cells through blocking synthesis of retinoic acid in Medaka (Oryzias latipes)</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-08-06</date><risdate>2013</risdate><volume>8</volume><issue>8</issue><spage>e70670</spage><epage>e70670</epage><pages>e70670-e70670</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Application of nanosecond pulsed electric fields (nsPEFs) has attracted rising attention in various scientific fields including medical, pharmacological, and biological sciences, although its effects and molecular mechanisms leading to the effects remain poorly understood. Here, we show that a single, high-intensity (10-30 kV/cm), 60-ns PEF exposure affects gene expression and impairs development of eyes and germ cells in medaka (Oryzias latipes). Exposure of early blastula stage embryos to nsPEF down-regulated the expression of several transcription factors which are essential for eye development, causing abnormal eye formation. Moreover, the majority of the exposed genetic female embryos showed a fewer number of germ cells similar to that of the control (unexposed) genetic male at 9 days post-fertilization (dpf). However, all-trans retinoic acid (atRA) treatment following the exposure rescued proliferation of germ cells and resumption of normal eye development, suggesting that the phenotypes induced by nsPEF are caused by a decrease of retinoic acid levels. These results confirm that nsPEFs induce novel effects during embryogenesis in medaka.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23936463</pmid><doi>10.1371/journal.pone.0070670</doi><tpages>e70670</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acids Animals Biological effects Biology Blastula Cell Proliferation Dehydrogenases Deoxyribonucleic acid DNA DNA binding proteins Electric fields Electroporation - methods Embryogenesis Embryonic Development Embryonic growth stage Embryos Enzymes Exposure Eye Eye - embryology Eye - metabolism Female Fertilization Gene expression Gene Expression Regulation, Developmental Genes Genetic engineering Genomes Germ cells Male Medicine Metabolism Molecular modelling Mutation Nuclear Proteins - genetics Oryzias - embryology Oryzias latipes Ovum - cytology Ovum - metabolism Pharmacology Physics Retinoic acid RNA, Messenger - genetics RNA, Messenger - metabolism Rodents Science Spermatozoa - cytology Spermatozoa - metabolism Studies Time Factors Transcription factors Tretinoin Tretinoin - metabolism Zebrafish |
| title | Nanosecond pulsed electric field suppresses development of eyes and germ cells through blocking synthesis of retinoic acid in Medaka (Oryzias latipes) |
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