Human voltage-gated proton channel hv1: a new potential biomarker for diagnosis and prognosis of colorectal cancer
Solid tumors exist in a hypoxic microenvironment, and possess high-glycolytic metabolites. To avoid the acidosis, tumor cells must exhibit a dynamic cytosolic pH regulation mechanism(s). The voltage-gated proton channel Hv1 mediates NADPH oxidase function by compensating cellular loss of electrons w...
Gespeichert in:
| Veröffentlicht in: | PloS one 2013-08, Vol.8 (8), p.e70550-e70550 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e70550 |
|---|---|
| container_issue | 8 |
| container_start_page | e70550 |
| container_title | PloS one |
| container_volume | 8 |
| creator | Wang, Yifan Wu, Xingye Li, Qiang Zhang, Shangrong Li, Shu Jie |
| description | Solid tumors exist in a hypoxic microenvironment, and possess high-glycolytic metabolites. To avoid the acidosis, tumor cells must exhibit a dynamic cytosolic pH regulation mechanism(s). The voltage-gated proton channel Hv1 mediates NADPH oxidase function by compensating cellular loss of electrons with protons. Here, we showed for the first time, that Hv1 expression is increased in colorectal tumor tissues and cell lines, associated with poor prognosis. Immunohistochemistry showed that Hv1 is strongly expressed in adenocarcinomas but not or lowly expressed in normal colorectal or hyperplastic polyps. Hv1 expression in colorectal cancer is significantly associated with the tumor size, tumor classification, lymph node status, clinical stage and p53 status. High Hv1 expression is associated significantly with shorter overall and recurrence-free survival. Furthermore, real-time RT-PCR and immunocytochemistry showed that Hv1 is highly expressed in colorectal cancer cell lines, SW620, HT29, LS174T and Colo205, but not in SW480. Inhibitions of Hv1 expression and activity in the highly metastatic SW620 cells by small interfering RNA (siRNA) and Zn(2+) respectively, markedly decrease the cell invasion and migration, restraint proton extrusion and the intracellular pH recovery. Our results suggest that Hv1 may be used as a potential biomarker for diagnosis and prognosis of colorectal carcinoma, and a potential target for anticancer drugs in colorectal cancer therapy. |
| doi_str_mv | 10.1371/journal.pone.0070550 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1430247316</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478419542</galeid><doaj_id>oai_doaj_org_article_158edd4622584721b802255c4052c07b</doaj_id><sourcerecordid>A478419542</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-2746b39ef74ffa664c49c16eb55d716f99925fb3467f1dc475db752d799a51c93</originalsourceid><addsrcrecordid>eNqNk11v0zAUhiMEYmPwDxBEQkJw0eJvx1wgTROwSpMm8XVrOY6Turh2ZzsD_j3umk0t2gXKRWzned-T8yanqp5DMIeYw3erMEav3HwTvJkDwAGl4EF1DAVGM4YAfri3PqqepLQCgOKGscfVEcKCACrgcRXPx7Xy9XVwWQ1mNqhsunoTQw6-1kvlvXH18hq-r1Xtza96E7Lx2SpXtzasVfxpYt2HWHdWDT4km2rlb_TTLvS1Di5Eo3PRaOW1iU-rR71yyTyb7ifV908fv52dzy4uPy_OTi9mmtMmzxAnrMXC9Jz0vWKMaCI0ZKaltOOQ9UIIRPsWE8Z72GnCaddyijouhKJQC3xSvdz5blxIcoorSUgwQIRjyAqx2BFdUCu5ibZ09EcGZeXNQYiDVDFb7YyEtDFdRxhCtCEcwbYBZUl1iRFpwNvi9WGqNrZr0-kSU1TuwPTwibdLOYRriTkmqEHF4M1kEMPVaFKWa5u0cU55E8bteyPAIMaMFvTVP-j93U3UoEoD1veh1NVbU3lKeEOgoGRbdn4PVa7OrK0u_1Zvy_mB4O2BoDDZ_M6DGlOSi69f_p-9_HHIvt5jl0a5vEzBjdkGnw5BsgN1DClF09-FDIHcjsZtGnI7GnIajSJ7sf-B7kS3s4D_AsIfCF8</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1430247316</pqid></control><display><type>article</type><title>Human voltage-gated proton channel hv1: a new potential biomarker for diagnosis and prognosis of colorectal cancer</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Wang, Yifan ; Wu, Xingye ; Li, Qiang ; Zhang, Shangrong ; Li, Shu Jie</creator><contributor>Srivastava, Rakesh K.</contributor><creatorcontrib>Wang, Yifan ; Wu, Xingye ; Li, Qiang ; Zhang, Shangrong ; Li, Shu Jie ; Srivastava, Rakesh K.</creatorcontrib><description>Solid tumors exist in a hypoxic microenvironment, and possess high-glycolytic metabolites. To avoid the acidosis, tumor cells must exhibit a dynamic cytosolic pH regulation mechanism(s). The voltage-gated proton channel Hv1 mediates NADPH oxidase function by compensating cellular loss of electrons with protons. Here, we showed for the first time, that Hv1 expression is increased in colorectal tumor tissues and cell lines, associated with poor prognosis. Immunohistochemistry showed that Hv1 is strongly expressed in adenocarcinomas but not or lowly expressed in normal colorectal or hyperplastic polyps. Hv1 expression in colorectal cancer is significantly associated with the tumor size, tumor classification, lymph node status, clinical stage and p53 status. High Hv1 expression is associated significantly with shorter overall and recurrence-free survival. Furthermore, real-time RT-PCR and immunocytochemistry showed that Hv1 is highly expressed in colorectal cancer cell lines, SW620, HT29, LS174T and Colo205, but not in SW480. Inhibitions of Hv1 expression and activity in the highly metastatic SW620 cells by small interfering RNA (siRNA) and Zn(2+) respectively, markedly decrease the cell invasion and migration, restraint proton extrusion and the intracellular pH recovery. Our results suggest that Hv1 may be used as a potential biomarker for diagnosis and prognosis of colorectal carcinoma, and a potential target for anticancer drugs in colorectal cancer therapy.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0070550</identifier><identifier>PMID: 23940591</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acidification ; Acidosis ; Adult ; Aged ; Antineoplastic drugs ; Antitumor agents ; Apoptosis ; Bioindicators ; Biology ; Biomarkers ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Biophysics ; Biotechnology ; Blotting, Western ; Breast cancer ; Cancer ; Cancer metastasis ; Cell Line ; Cell migration ; Cell Movement - genetics ; Cell Movement - physiology ; Chemotherapy ; Colorectal cancer ; Colorectal carcinoma ; Colorectal Neoplasms - diagnosis ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - metabolism ; Diagnosis ; Drugs ; Electric potential ; Ethics ; Extrusion ; Female ; Fluorescent Antibody Technique ; Gastrointestinal diseases ; Glycolysis ; Health aspects ; Humans ; Hydrogen-Ion Concentration ; Hypoxia ; Immunocytochemistry ; Immunohistochemistry ; Ion Channels - genetics ; Ion Channels - metabolism ; Lymph nodes ; Male ; Medical diagnosis ; Medical prognosis ; Medicine ; Metabolites ; Metastases ; Metastasis ; Middle Aged ; NAD(P)H oxidase ; Neutrophils ; Oxidases ; p53 Protein ; Pathology ; Patients ; pH effects ; Physics ; Polyclonal antibodies ; Polymerase chain reaction ; Polyps ; Prognosis ; Proteins ; Protons ; Real-Time Polymerase Chain Reaction ; Ribonucleic acid ; RNA ; Rodents ; siRNA ; Solid tumors ; Surgery ; Tissues ; Tumor cell lines ; Tumor cells ; Tumor proteins ; Tumors ; Voltage</subject><ispartof>PloS one, 2013-08, Vol.8 (8), p.e70550-e70550</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Wang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Wang et al 2013 Wang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-2746b39ef74ffa664c49c16eb55d716f99925fb3467f1dc475db752d799a51c93</citedby><cites>FETCH-LOGICAL-c758t-2746b39ef74ffa664c49c16eb55d716f99925fb3467f1dc475db752d799a51c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734282/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734282/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23940591$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Srivastava, Rakesh K.</contributor><creatorcontrib>Wang, Yifan</creatorcontrib><creatorcontrib>Wu, Xingye</creatorcontrib><creatorcontrib>Li, Qiang</creatorcontrib><creatorcontrib>Zhang, Shangrong</creatorcontrib><creatorcontrib>Li, Shu Jie</creatorcontrib><title>Human voltage-gated proton channel hv1: a new potential biomarker for diagnosis and prognosis of colorectal cancer</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Solid tumors exist in a hypoxic microenvironment, and possess high-glycolytic metabolites. To avoid the acidosis, tumor cells must exhibit a dynamic cytosolic pH regulation mechanism(s). The voltage-gated proton channel Hv1 mediates NADPH oxidase function by compensating cellular loss of electrons with protons. Here, we showed for the first time, that Hv1 expression is increased in colorectal tumor tissues and cell lines, associated with poor prognosis. Immunohistochemistry showed that Hv1 is strongly expressed in adenocarcinomas but not or lowly expressed in normal colorectal or hyperplastic polyps. Hv1 expression in colorectal cancer is significantly associated with the tumor size, tumor classification, lymph node status, clinical stage and p53 status. High Hv1 expression is associated significantly with shorter overall and recurrence-free survival. Furthermore, real-time RT-PCR and immunocytochemistry showed that Hv1 is highly expressed in colorectal cancer cell lines, SW620, HT29, LS174T and Colo205, but not in SW480. Inhibitions of Hv1 expression and activity in the highly metastatic SW620 cells by small interfering RNA (siRNA) and Zn(2+) respectively, markedly decrease the cell invasion and migration, restraint proton extrusion and the intracellular pH recovery. Our results suggest that Hv1 may be used as a potential biomarker for diagnosis and prognosis of colorectal carcinoma, and a potential target for anticancer drugs in colorectal cancer therapy.</description><subject>Acidification</subject><subject>Acidosis</subject><subject>Adult</subject><subject>Aged</subject><subject>Antineoplastic drugs</subject><subject>Antitumor agents</subject><subject>Apoptosis</subject><subject>Bioindicators</subject><subject>Biology</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Biophysics</subject><subject>Biotechnology</subject><subject>Blotting, Western</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Cancer metastasis</subject><subject>Cell Line</subject><subject>Cell migration</subject><subject>Cell Movement - genetics</subject><subject>Cell Movement - physiology</subject><subject>Chemotherapy</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Colorectal Neoplasms - diagnosis</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Diagnosis</subject><subject>Drugs</subject><subject>Electric potential</subject><subject>Ethics</subject><subject>Extrusion</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Gastrointestinal diseases</subject><subject>Glycolysis</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hypoxia</subject><subject>Immunocytochemistry</subject><subject>Immunohistochemistry</subject><subject>Ion Channels - genetics</subject><subject>Ion Channels - metabolism</subject><subject>Lymph nodes</subject><subject>Male</subject><subject>Medical diagnosis</subject><subject>Medical prognosis</subject><subject>Medicine</subject><subject>Metabolites</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Middle Aged</subject><subject>NAD(P)H oxidase</subject><subject>Neutrophils</subject><subject>Oxidases</subject><subject>p53 Protein</subject><subject>Pathology</subject><subject>Patients</subject><subject>pH effects</subject><subject>Physics</subject><subject>Polyclonal antibodies</subject><subject>Polymerase chain reaction</subject><subject>Polyps</subject><subject>Prognosis</subject><subject>Proteins</subject><subject>Protons</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Rodents</subject><subject>siRNA</subject><subject>Solid tumors</subject><subject>Surgery</subject><subject>Tissues</subject><subject>Tumor cell lines</subject><subject>Tumor cells</subject><subject>Tumor proteins</subject><subject>Tumors</subject><subject>Voltage</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBEQkJw0eJvx1wgTROwSpMm8XVrOY6Turh2ZzsD_j3umk0t2gXKRWzned-T8yanqp5DMIeYw3erMEav3HwTvJkDwAGl4EF1DAVGM4YAfri3PqqepLQCgOKGscfVEcKCACrgcRXPx7Xy9XVwWQ1mNqhsunoTQw6-1kvlvXH18hq-r1Xtza96E7Lx2SpXtzasVfxpYt2HWHdWDT4km2rlb_TTLvS1Di5Eo3PRaOW1iU-rR71yyTyb7ifV908fv52dzy4uPy_OTi9mmtMmzxAnrMXC9Jz0vWKMaCI0ZKaltOOQ9UIIRPsWE8Z72GnCaddyijouhKJQC3xSvdz5blxIcoorSUgwQIRjyAqx2BFdUCu5ibZ09EcGZeXNQYiDVDFb7YyEtDFdRxhCtCEcwbYBZUl1iRFpwNvi9WGqNrZr0-kSU1TuwPTwibdLOYRriTkmqEHF4M1kEMPVaFKWa5u0cU55E8bteyPAIMaMFvTVP-j93U3UoEoD1veh1NVbU3lKeEOgoGRbdn4PVa7OrK0u_1Zvy_mB4O2BoDDZ_M6DGlOSi69f_p-9_HHIvt5jl0a5vEzBjdkGnw5BsgN1DClF09-FDIHcjsZtGnI7GnIajSJ7sf-B7kS3s4D_AsIfCF8</recordid><startdate>20130805</startdate><enddate>20130805</enddate><creator>Wang, Yifan</creator><creator>Wu, Xingye</creator><creator>Li, Qiang</creator><creator>Zhang, Shangrong</creator><creator>Li, Shu Jie</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130805</creationdate><title>Human voltage-gated proton channel hv1: a new potential biomarker for diagnosis and prognosis of colorectal cancer</title><author>Wang, Yifan ; Wu, Xingye ; Li, Qiang ; Zhang, Shangrong ; Li, Shu Jie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-2746b39ef74ffa664c49c16eb55d716f99925fb3467f1dc475db752d799a51c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acidification</topic><topic>Acidosis</topic><topic>Adult</topic><topic>Aged</topic><topic>Antineoplastic drugs</topic><topic>Antitumor agents</topic><topic>Apoptosis</topic><topic>Bioindicators</topic><topic>Biology</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Biophysics</topic><topic>Biotechnology</topic><topic>Blotting, Western</topic><topic>Breast cancer</topic><topic>Cancer</topic><topic>Cancer metastasis</topic><topic>Cell Line</topic><topic>Cell migration</topic><topic>Cell Movement - genetics</topic><topic>Cell Movement - physiology</topic><topic>Chemotherapy</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Colorectal Neoplasms - diagnosis</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - metabolism</topic><topic>Diagnosis</topic><topic>Drugs</topic><topic>Electric potential</topic><topic>Ethics</topic><topic>Extrusion</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Gastrointestinal diseases</topic><topic>Glycolysis</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hypoxia</topic><topic>Immunocytochemistry</topic><topic>Immunohistochemistry</topic><topic>Ion Channels - genetics</topic><topic>Ion Channels - metabolism</topic><topic>Lymph nodes</topic><topic>Male</topic><topic>Medical diagnosis</topic><topic>Medical prognosis</topic><topic>Medicine</topic><topic>Metabolites</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Middle Aged</topic><topic>NAD(P)H oxidase</topic><topic>Neutrophils</topic><topic>Oxidases</topic><topic>p53 Protein</topic><topic>Pathology</topic><topic>Patients</topic><topic>pH effects</topic><topic>Physics</topic><topic>Polyclonal antibodies</topic><topic>Polymerase chain reaction</topic><topic>Polyps</topic><topic>Prognosis</topic><topic>Proteins</topic><topic>Protons</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Rodents</topic><topic>siRNA</topic><topic>Solid tumors</topic><topic>Surgery</topic><topic>Tissues</topic><topic>Tumor cell lines</topic><topic>Tumor cells</topic><topic>Tumor proteins</topic><topic>Tumors</topic><topic>Voltage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yifan</creatorcontrib><creatorcontrib>Wu, Xingye</creatorcontrib><creatorcontrib>Li, Qiang</creatorcontrib><creatorcontrib>Zhang, Shangrong</creatorcontrib><creatorcontrib>Li, Shu Jie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yifan</au><au>Wu, Xingye</au><au>Li, Qiang</au><au>Zhang, Shangrong</au><au>Li, Shu Jie</au><au>Srivastava, Rakesh K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human voltage-gated proton channel hv1: a new potential biomarker for diagnosis and prognosis of colorectal cancer</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-08-05</date><risdate>2013</risdate><volume>8</volume><issue>8</issue><spage>e70550</spage><epage>e70550</epage><pages>e70550-e70550</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Solid tumors exist in a hypoxic microenvironment, and possess high-glycolytic metabolites. To avoid the acidosis, tumor cells must exhibit a dynamic cytosolic pH regulation mechanism(s). The voltage-gated proton channel Hv1 mediates NADPH oxidase function by compensating cellular loss of electrons with protons. Here, we showed for the first time, that Hv1 expression is increased in colorectal tumor tissues and cell lines, associated with poor prognosis. Immunohistochemistry showed that Hv1 is strongly expressed in adenocarcinomas but not or lowly expressed in normal colorectal or hyperplastic polyps. Hv1 expression in colorectal cancer is significantly associated with the tumor size, tumor classification, lymph node status, clinical stage and p53 status. High Hv1 expression is associated significantly with shorter overall and recurrence-free survival. Furthermore, real-time RT-PCR and immunocytochemistry showed that Hv1 is highly expressed in colorectal cancer cell lines, SW620, HT29, LS174T and Colo205, but not in SW480. Inhibitions of Hv1 expression and activity in the highly metastatic SW620 cells by small interfering RNA (siRNA) and Zn(2+) respectively, markedly decrease the cell invasion and migration, restraint proton extrusion and the intracellular pH recovery. Our results suggest that Hv1 may be used as a potential biomarker for diagnosis and prognosis of colorectal carcinoma, and a potential target for anticancer drugs in colorectal cancer therapy.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23940591</pmid><doi>10.1371/journal.pone.0070550</doi><tpages>e70550</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-08, Vol.8 (8), p.e70550-e70550 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1430247316 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acidification Acidosis Adult Aged Antineoplastic drugs Antitumor agents Apoptosis Bioindicators Biology Biomarkers Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Biophysics Biotechnology Blotting, Western Breast cancer Cancer Cancer metastasis Cell Line Cell migration Cell Movement - genetics Cell Movement - physiology Chemotherapy Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - diagnosis Colorectal Neoplasms - genetics Colorectal Neoplasms - metabolism Diagnosis Drugs Electric potential Ethics Extrusion Female Fluorescent Antibody Technique Gastrointestinal diseases Glycolysis Health aspects Humans Hydrogen-Ion Concentration Hypoxia Immunocytochemistry Immunohistochemistry Ion Channels - genetics Ion Channels - metabolism Lymph nodes Male Medical diagnosis Medical prognosis Medicine Metabolites Metastases Metastasis Middle Aged NAD(P)H oxidase Neutrophils Oxidases p53 Protein Pathology Patients pH effects Physics Polyclonal antibodies Polymerase chain reaction Polyps Prognosis Proteins Protons Real-Time Polymerase Chain Reaction Ribonucleic acid RNA Rodents siRNA Solid tumors Surgery Tissues Tumor cell lines Tumor cells Tumor proteins Tumors Voltage |
| title | Human voltage-gated proton channel hv1: a new potential biomarker for diagnosis and prognosis of colorectal cancer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T09%3A39%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20voltage-gated%20proton%20channel%20hv1:%20a%20new%20potential%20biomarker%20for%20diagnosis%20and%20prognosis%20of%20colorectal%20cancer&rft.jtitle=PloS%20one&rft.au=Wang,%20Yifan&rft.date=2013-08-05&rft.volume=8&rft.issue=8&rft.spage=e70550&rft.epage=e70550&rft.pages=e70550-e70550&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0070550&rft_dat=%3Cgale_plos_%3EA478419542%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1430247316&rft_id=info:pmid/23940591&rft_galeid=A478419542&rft_doaj_id=oai_doaj_org_article_158edd4622584721b802255c4052c07b&rfr_iscdi=true |