Measurement of the infection and dissemination of bluetongue virus in culicoides biting midges using a semi-quantitative rt-PCR assay and isolation of infectious virus

Culicoides biting midges (Diptera: Ceratopogonidae) are the biological vectors of globally significant arboviruses of livestock including bluetongue virus (BTV), African horse sickness virus (AHSV) and the recently emerging Schmallenberg virus (SBV). From 2006-2009 outbreaks of BTV in northern Europ...

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Veröffentlicht in:	PloS one 2013-08, Vol.8 (8), p.e70800-e70800
Hauptverfasser:	Veronesi, Eva, Antony, Frank, Gubbins, Simon, Golding, Nick, Blackwell, Alison, Mertens, Peter Pc, Brownlie, Joe, Darpel, Karin E, Mellor, Philip S, Carpenter, Simon
Format:	Artikel
Sprache:	eng
Schlagworte:	African horse sickness Analysis Animals Assaying Biology Biting Bluetongue Bluetongue - transmission Bluetongue - virology Bluetongue virus - genetics Bluetongue virus - isolation & purification Bluetongue virus - physiology Cell culture Ceratopogonidae Ceratopogonidae - virology Colonies & territories Culicoides Culicoides chiopterus Culicoides variipennis Diagnostic software Diagnostic systems Diptera Economic impact Embryos Enzyme-linked immunosorbent assay Genetic vectors Horses Infections Infectious diseases Insect Vectors - virology Laboratories Livestock Measurement Medicine Outbreaks Polymerase chain reaction Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Ribonucleic acid RNA RNA, Viral - genetics Species Studies Time series Vectors Vectors (Biology) Veterinary Science Viral genetics Viral Load Virus Replication Viruses West Nile virus
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